FastQCFastQC Report
Sat 18 Jun 2016
SRR3551371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511285
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG91271.7851100658145653No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC83791.6388120128695345No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC80501.5744643398495948No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40420.790557125673548No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC38160.7463547727783917No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT21120.4130768553742042No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGAGGTT18630.36437603293662046No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTA15570.3045268294591079No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT14900.2914225920963846No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC13720.26834348748740916No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC13420.2624759185190256No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT12220.2390056426454913Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC9990.1953900466471733No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT8490.16605220180525537No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8450.16526985927613758No Hit
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC7620.149036251796943No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC7470.1461024673127512No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5560.10874561154737573No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5300.10366038510810996No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5150.10072660062391817No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC207.029607E-445.00000433
CGATTGC207.029607E-445.00000410
CGCGACC207.029607E-445.00000410
AGACGTG207.029607E-445.00000424
CCCGTTA207.029607E-445.00000426
CGGATTA207.029607E-445.00000422
CGTAGTG207.029607E-445.00000416
TTCCACG207.029607E-445.00000417
GTATGCG351.2101555E-745.0000041
CCGAGAG207.029607E-445.00000434
AAATTCG207.029607E-445.00000430
ACTCGTT207.029607E-445.00000431
CGTACAT207.029607E-445.00000435
ATGCGTT207.029607E-445.00000427
AACTCGC207.029607E-445.00000439
TGGGCGC207.029607E-445.0000046
GGACCGT207.029607E-445.0000048
CCAACGA351.2101555E-745.00000437
ATCGGCT207.029607E-445.00000429
CGCAAAG207.029607E-445.00000424