FastQCFastQC Report
Sat 18 Jun 2016
SRR3551368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732624
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.42736792679464497No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC21780.29728755814715324No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT10170.13881609120094346No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9460.12912489899320798No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC9400.12830592500382187No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC8590.11724977614710956No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8590.11724977614710956No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC8250.11260892354058834No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC7770.10605713162549957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.031609E-445.00000410
TCGCAAG207.031609E-445.0000042
ACGCATC207.031609E-445.00000425
GCGCGTA207.031609E-445.0000041
ATAGCGG406.8084773E-945.0000042
TTAAGCG207.031609E-445.0000041
CGTCGAC207.031609E-445.00000431
ATCGGTT207.031609E-445.00000424
AATAGCG207.031609E-445.0000041
CCACGTA207.031609E-445.00000427
TGCGTAG406.8084773E-945.0000041
GTCGACT253.889416E-545.032
CCCACCG253.889416E-545.036
GCCGATA253.889416E-545.09
TCTACGG453.8380676E-1045.02
CGGATTA253.889416E-545.06
TGCGACG302.164421E-644.9999961
ACTAACG302.164421E-644.9999961
CTACCGA302.164421E-644.99999625
AATCGAA302.164421E-644.99999629