##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551368_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732624
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.641767127476033	31.0	31.0	34.0	30.0	34.0
2	31.777767313110136	33.0	31.0	34.0	30.0	34.0
3	31.73502233069078	33.0	31.0	34.0	30.0	34.0
4	35.43995009718491	37.0	35.0	37.0	33.0	37.0
5	33.34783736268536	37.0	35.0	37.0	30.0	37.0
6	34.29634983292931	37.0	35.0	37.0	30.0	37.0
7	35.41588318155016	37.0	35.0	37.0	32.0	37.0
8	35.33788546375767	37.0	35.0	37.0	33.0	37.0
9	37.225516772587305	39.0	37.0	39.0	34.0	39.0
10	36.70032240276049	39.0	35.0	39.0	32.0	39.0
11	36.801381063137434	39.0	37.0	39.0	33.0	39.0
12	36.79764927165913	39.0	35.0	39.0	33.0	39.0
13	36.88438271200506	39.0	37.0	39.0	33.0	39.0
14	37.900023750245694	40.0	37.0	41.0	33.0	41.0
15	38.04824029788814	40.0	37.0	41.0	33.0	41.0
16	38.08374418528467	40.0	37.0	41.0	33.0	41.0
17	37.991998624123696	40.0	37.0	41.0	33.0	41.0
18	37.950644532529644	40.0	37.0	41.0	33.0	41.0
19	37.86777937932691	40.0	37.0	41.0	33.0	41.0
20	38.04502173010985	40.0	37.0	41.0	34.0	41.0
21	37.89797495031558	40.0	36.0	41.0	33.0	41.0
22	38.02650199829653	40.0	37.0	41.0	34.0	41.0
23	38.1265969992793	40.0	37.0	41.0	34.0	41.0
24	37.98416786782852	40.0	36.0	41.0	34.0	41.0
25	37.63946035073926	39.0	36.0	41.0	33.0	41.0
26	37.7656710672869	40.0	36.0	41.0	33.0	41.0
27	37.3942800126668	40.0	36.0	41.0	33.0	41.0
28	37.435504433379194	40.0	36.0	41.0	33.0	41.0
29	37.635235809910675	40.0	36.0	41.0	33.0	41.0
30	37.387534396907554	39.0	36.0	41.0	33.0	41.0
31	37.34756164144227	39.0	35.0	41.0	33.0	41.0
32	37.48503871017056	40.0	36.0	41.0	33.0	41.0
33	37.49080838192579	40.0	36.0	41.0	33.0	41.0
34	37.38149719364913	40.0	36.0	41.0	33.0	41.0
35	37.36824209963092	40.0	36.0	41.0	33.0	41.0
36	37.16520889296556	40.0	35.0	41.0	32.0	41.0
37	37.16278745987027	40.0	35.0	41.0	32.0	41.0
38	37.0048073773177	39.0	35.0	41.0	32.0	41.0
39	36.949061182816834	39.0	35.0	41.0	32.0	41.0
40	36.83708832907467	39.0	35.0	41.0	31.0	41.0
41	36.657842221931034	39.0	35.0	41.0	31.0	41.0
42	36.83779128174889	39.0	35.0	41.0	31.0	41.0
43	36.75274629277774	39.0	35.0	41.0	31.0	41.0
44	36.79875898141475	39.0	35.0	41.0	31.0	41.0
45	36.80464330952849	39.0	35.0	41.0	32.0	41.0
46	36.69070355325515	39.0	35.0	41.0	31.0	41.0
47	36.675948371879706	39.0	35.0	41.0	31.0	41.0
48	36.60680922273909	39.0	35.0	41.0	31.0	41.0
49	36.53849723732774	39.0	35.0	41.0	31.0	41.0
50	36.4577081831881	39.0	35.0	41.0	31.0	41.0
51	35.58377694424425	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	8.0
8	11.0
9	47.0
10	44.0
11	57.0
12	37.0
13	50.0
14	57.0
15	67.0
16	105.0
17	155.0
18	229.0
19	386.0
20	647.0
21	1027.0
22	1574.0
23	2208.0
24	3130.0
25	4583.0
26	6000.0
27	7260.0
28	7909.0
29	8927.0
30	10816.0
31	13804.0
32	18590.0
33	26534.0
34	47635.0
35	57764.0
36	60713.0
37	89257.0
38	151203.0
39	211729.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08748280154622	21.391054620105265	23.62275874118238	12.898703837166131
2	33.59636047959117	24.14799405970866	24.27998536766472	17.975660093035444
3	32.23194981327393	23.099161370634867	27.668763240079496	17.00012557601171
4	29.6831662626395	25.025524689335864	26.110938216602243	19.180370831422394
5	24.455518792723144	34.294535805542814	23.28411299657123	17.965832405162814
6	27.061767018279497	33.89583196837668	24.264697853196182	14.777703160147635
7	78.4572986961934	7.006458974862959	9.658160256830243	4.87808207211339
8	79.69313044617704	6.5325460263382045	9.010215335560941	4.764108191923825
9	72.00760553844809	9.860037345213916	11.617692022101378	6.514665094236608
10	39.96920657799908	28.677739195003166	18.698541134333574	12.654513092664176
11	31.170832514359343	26.32619188014589	24.500835353469174	18.002140252025594
12	29.67907139269257	22.672202930834917	28.21952324794165	19.42920242853087
13	25.42190810019874	24.98457598986656	28.603076066304133	20.990439843630565
14	19.230328244774945	29.11384284435126	28.817374260193496	22.838454650680294
15	19.396033981960734	27.381712856799666	33.688631549062	19.533621612177598
16	20.85940400532879	26.666066085741118	32.257065015615105	20.217464893314986
17	22.03517766275743	26.007474502609796	28.31602568302431	23.641322151608467
18	22.66374019961126	27.535133984144665	28.918381052217786	20.882744764026295
19	25.238457926576253	27.16413876695276	27.365879359671535	20.23152394679945
20	26.717251960077746	26.191470658891873	28.309200899779423	18.782076481250957
21	25.724109502282204	26.377377754482517	28.00085719277556	19.897655550459717
22	23.0325514861648	26.03845901854157	27.645422481381992	23.28356701391164
23	22.76392801764616	26.744551093057282	28.611265806197995	21.88025508309856
24	22.856199087116995	25.27148987748149	29.75318853873201	22.119122496669505
25	22.463501059206358	27.615120443774703	26.780722444255172	23.140656052763763
26	21.311341151805017	29.431058769573475	26.776081591648648	22.481518486972853
27	21.56699753215838	30.411916617528227	27.749978160693615	20.27110768961978
28	20.774776693092228	28.42003537967634	29.012153574002493	21.79303435322894
29	21.087897748367514	27.48190067483457	29.210891262093515	22.219310314704405
30	21.751676166764945	27.890295704208434	28.080707156740704	22.27732097228592
31	21.84558518421455	28.30114765555046	25.91656838978794	23.93669877044705
32	22.45640328463168	28.337455502413246	26.709335211513686	22.496806001441392
33	22.634530127323156	28.49838389132761	26.723940247657733	22.143145733691497
34	21.050224944855753	26.8201696914106	27.516161086723883	24.613444277009762
35	21.72983686038131	26.296845312192886	26.74072921444015	25.23258861298565
36	21.351607372949836	28.59161043045273	25.980311865295157	24.07647033130228
37	21.106188167463802	28.16492498198257	27.538000393107513	23.190886457446112
38	20.82009325383826	26.925407849046714	27.68446024154273	24.570038655572297
39	22.84623490357946	24.850537246937037	29.010106139029023	23.293121710454475
40	22.860839939723515	25.03193998558606	28.991269737273146	23.115950337417285
41	20.005896612723582	26.204301251392252	28.091763305597418	25.69803883028675
42	21.824564851820305	26.723257769333248	26.936463997903427	24.515713380943023
43	22.857564043765972	27.22556181615672	27.35263928017646	22.56423485990085
44	21.29004782808098	26.65637489353338	27.451325645897484	24.602251632488155
45	20.92410295049029	26.21044355631265	26.764069973137655	26.1013835200594
46	20.051213173469613	27.733598680905896	27.467295638690516	24.74789250693398
47	20.98716394767302	25.93021795627771	29.305755749197402	23.776862346851864
48	20.7881532682522	25.079849963965145	29.46804909476075	24.663947673021905
49	21.15887549411431	25.205016488676318	29.349297866299768	24.286810150909606
50	19.17163510886894	25.304931315381424	30.100706501561508	25.422727074188124
51	19.84496822380921	25.537519928367075	27.627541549280394	26.98997029854332
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	506.0
1	951.5
2	1397.0
3	3714.5
4	6032.0
5	4276.5
6	2521.0
7	2404.5
8	2288.0
9	2243.0
10	2198.0
11	2116.0
12	2034.0
13	2073.0
14	2112.0
15	2115.0
16	2118.0
17	2043.0
18	1968.0
19	2129.0
20	2290.0
21	2428.0
22	2566.0
23	3045.5
24	3525.0
25	4235.5
26	6316.0
27	7686.0
28	9079.0
29	10472.0
30	11935.0
31	13398.0
32	15310.5
33	17223.0
34	19594.5
35	21966.0
36	23969.0
37	25972.0
38	27385.5
39	28799.0
40	31377.0
41	33955.0
42	37011.0
43	40067.0
44	42897.5
45	45728.0
46	47747.0
47	49766.0
48	52839.5
49	55913.0
50	59329.0
51	62745.0
52	60657.5
53	58570.0
54	52103.5
55	45637.0
56	41487.5
57	37338.0
58	36106.5
59	34875.0
60	33678.0
61	32481.0
62	28859.5
63	25238.0
64	21390.0
65	17542.0
66	14431.0
67	11320.0
68	9365.0
69	7410.0
70	6190.0
71	4970.0
72	4099.0
73	3228.0
74	2483.5
75	1373.0
76	1007.0
77	827.0
78	647.0
79	450.0
80	253.0
81	177.0
82	101.0
83	83.0
84	65.0
85	35.5
86	6.0
87	3.0
88	0.0
89	1.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	732624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.93954061206343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5010610565099	20.070243599736653
2	10.459756059993937	5.635620463409664
3	3.8693135614206673	3.127125894860995
4	1.9324765736797482	2.0824012455402703
5	1.0974876649960286	1.4782906761199586
6	0.7152422404814669	1.1560978430948228
7	0.5229359651852135	0.986135827013095
8	0.37305044986229924	0.8039846195531152
9	0.2917621658677359	0.707394484481171
>10	4.726355450500494	33.74279386978487
>50	1.43121736257576	24.79368365814054
>100	0.07166324419189976	3.2870795250141263
>500	0.006142563787877122	1.2568042451222363
>1k	0.0015356409469692806	0.8723440481285129
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3131	0.42736792679464497	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	2178	0.29728755814715324	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	1017	0.13881609120094346	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	946	0.12912489899320798	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC	940	0.12830592500382187	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCC	859	0.11724977614710956	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	859	0.11724977614710956	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	825	0.11260892354058834	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	777	0.10605713162549957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3649566489768285E-4	0.0	0.0	0.14523138745113456	0.0
2	1.3649566489768285E-4	0.0	0.0	0.756595470527856	0.0
3	1.3649566489768285E-4	0.0	0.0	1.0006497193649129	0.0
4	1.3649566489768285E-4	0.0	0.0	1.3227794885234445	0.0
5	1.3649566489768285E-4	0.0	0.0	2.2101378060232806	0.0
6	1.3649566489768285E-4	0.0	0.0	2.7482037170499467	0.0
7	1.3649566489768285E-4	0.0	0.0	3.2387691366922184	0.0
8	1.3649566489768285E-4	0.0	0.0	4.010106139029024	0.0
9	2.729913297953657E-4	0.0	0.0	4.304390792548428	0.0
10	2.729913297953657E-4	0.0	0.0	5.055526436480378	0.0
11	2.729913297953657E-4	0.0	0.0	6.129474327895346	0.0
12	2.729913297953657E-4	0.0	0.0	7.013147262442945	0.0
13	2.729913297953657E-4	0.0	0.0	7.456758173360414	0.0
14	2.729913297953657E-4	0.0	0.0	7.675151237196706	0.0
15	2.729913297953657E-4	0.0	0.0	7.958243246194501	0.0
16	2.729913297953657E-4	0.0	0.0	8.501495992487278	0.0
17	2.729913297953657E-4	0.0	0.0	9.122960754766428	0.0
18	2.729913297953657E-4	0.0	0.0	9.850209657341283	0.0
19	2.729913297953657E-4	0.0	0.0	10.25915066937474	0.0
20	2.729913297953657E-4	0.0	0.0	10.71927755574483	0.0
21	2.729913297953657E-4	0.0	0.0	11.219261176265041	0.0
22	2.729913297953657E-4	0.0	0.0	11.771932123435759	0.0
23	2.729913297953657E-4	0.0	0.0	12.307131625499574	0.0
24	2.729913297953657E-4	0.0	0.0	12.727538273384438	0.0
25	2.729913297953657E-4	0.0	0.0	13.094984603289	0.0
26	2.729913297953657E-4	0.0	0.0	13.431992399921379	0.0
27	2.729913297953657E-4	0.0	0.0	13.778145406101903	0.0
28	2.729913297953657E-4	0.0	0.0	14.142452335713818	0.0
29	2.729913297953657E-4	0.0	0.0	14.525868658411408	0.0
30	4.0948699469304855E-4	0.0	0.0	14.959105898796654	0.0
31	4.0948699469304855E-4	0.0	0.0	15.365180501867261	0.0
32	5.459826595907314E-4	0.0	0.0	15.784222193103147	0.0
33	5.459826595907314E-4	0.0	0.0	16.157128349603617	0.0
34	5.459826595907314E-4	0.0	0.0	16.559654065386884	0.0
35	5.459826595907314E-4	0.0	0.0	16.947165258031404	0.0
36	5.459826595907314E-4	0.0	0.0	17.348189521500796	0.0
37	5.459826595907314E-4	0.0	0.0	17.743071480049792	0.0
38	5.459826595907314E-4	0.0	0.0	18.120754984821684	0.0
39	6.824783244884143E-4	0.0	0.0	18.51085959509926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.031609E-4	45.000004	10
TCGCAAG	20	7.031609E-4	45.000004	2
ACGCATC	20	7.031609E-4	45.000004	25
GCGCGTA	20	7.031609E-4	45.000004	1
ATAGCGG	40	6.8084773E-9	45.000004	2
TTAAGCG	20	7.031609E-4	45.000004	1
CGTCGAC	20	7.031609E-4	45.000004	31
ATCGGTT	20	7.031609E-4	45.000004	24
AATAGCG	20	7.031609E-4	45.000004	1
CCACGTA	20	7.031609E-4	45.000004	27
TGCGTAG	40	6.8084773E-9	45.000004	1
GTCGACT	25	3.889416E-5	45.0	32
CCCACCG	25	3.889416E-5	45.0	36
GCCGATA	25	3.889416E-5	45.0	9
TCTACGG	45	3.8380676E-10	45.0	2
CGGATTA	25	3.889416E-5	45.0	6
TGCGACG	30	2.164421E-6	44.999996	1
ACTAACG	30	2.164421E-6	44.999996	1
CTACCGA	30	2.164421E-6	44.999996	25
AATCGAA	30	2.164421E-6	44.999996	29
>>END_MODULE