FastQCFastQC Report
Sat 18 Jun 2016
SRR3551366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349518
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG85920.636671759843144No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC82700.6128113889551677No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC79650.5902107270892274No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67370.49921527537980226No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC42260.31314884277201194No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATACGTT20620.1527952943198979No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC20370.1509427810521979No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18640.13812338923971373No Hit
GAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT18490.13701188127909372No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT17880.13249174890590565No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT15180.11248460561474542Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTA14980.11100259500058539No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC13560.10048031964004925No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCC13550.10040621910934126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAC253.8911676E-545.00000442
GCCAACG253.8911676E-545.0000041
CCGTGCG207.0337235E-445.01
TCCGTAC207.0337235E-445.044
ACGCGAT302.1657852E-644.99999632
CGCGATT302.1657852E-644.99999633
CGACGGT650.041.5384628
CGTTTTT33000.041.113641
GCGCGAC556.184564E-1140.9090929
TAGCGCG556.184564E-1140.9090921
GTACGAG1100.040.9090921
CGATCGA1650.040.9090941
TACGGCT10300.040.8495147
CCGATGA14300.039.80769318
CGCGCAA403.4588265E-739.37518
TCGTAAG800.039.3751
CGGGACG1450.038.7931066
CGATGAA15200.038.6348719
GCCGATA356.249098E-638.5714269
CGGTCTA700.038.57142631
Produced by FastQC (version 0.11.5)