FastQCFastQC Report
Sat 18 Jun 2016
SRR3551365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083211
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230512.128024918506182No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC19680.1816820545581609No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCC17310.15980266079277258No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT13970.1289684096634912No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC12300.11355128409885054No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC11110.10256542815757966No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT207.0331054E-445.0000042
TGCGTCG207.0331054E-445.0000042
CAATGCG302.1653868E-644.9999961
CGTTTTT103000.043.820391
CGTTATT6900.042.065221
ACACGAC501.0822987E-940.50000426
TATTGCG451.9281288E-840.0000041
TATAGCG451.9281288E-840.0000041
CGAATGG1300.039.807692
CGTAAGG1650.039.5454522
TACGTTG403.4579716E-739.3750041
CGAATAT1200.039.37499614
GCTACGA1200.039.37499610
CCGAATT356.2479576E-638.57142629
AATGCGG1250.037.82
CGGCATG301.1398787E-437.4999961
GCCGATT601.5643309E-1037.4999969
TGCTACG850.037.0588231
AAGGGCG3100.037.016135
GCGCGAC552.748493E-936.8181849