##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1083211
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71946370559383	33.0	31.0	34.0	30.0	34.0
2	31.904666773140228	33.0	31.0	34.0	30.0	34.0
3	31.797523289553006	33.0	31.0	34.0	30.0	34.0
4	35.48643616063722	37.0	35.0	37.0	33.0	37.0
5	33.52153089287313	37.0	35.0	37.0	30.0	37.0
6	34.40843289072951	37.0	35.0	37.0	30.0	37.0
7	35.457694761223806	37.0	35.0	37.0	32.0	37.0
8	35.49046123054511	37.0	35.0	37.0	33.0	37.0
9	37.54077829711847	39.0	37.0	39.0	35.0	39.0
10	36.95388525411946	39.0	37.0	39.0	33.0	39.0
11	36.988470390348695	39.0	37.0	39.0	33.0	39.0
12	37.01460195659018	39.0	37.0	39.0	33.0	39.0
13	37.15017388117366	39.0	37.0	39.0	34.0	39.0
14	38.2116485153862	40.0	38.0	41.0	33.0	41.0
15	38.32377810048089	40.0	38.0	41.0	34.0	41.0
16	38.36649738601251	40.0	38.0	41.0	34.0	41.0
17	38.220843399854694	40.0	38.0	41.0	34.0	41.0
18	38.072704209983094	39.0	37.0	41.0	34.0	41.0
19	37.859775242311976	39.0	37.0	41.0	34.0	41.0
20	37.89839098753613	39.0	35.0	41.0	34.0	41.0
21	37.7294312927029	39.0	35.0	41.0	33.0	41.0
22	37.85717094822708	39.0	35.0	41.0	34.0	41.0
23	37.9431975856966	40.0	35.0	41.0	34.0	41.0
24	37.81580135356823	40.0	35.0	41.0	34.0	41.0
25	37.48364815349918	39.0	35.0	41.0	33.0	41.0
26	37.59485455742233	39.0	35.0	41.0	33.0	41.0
27	37.25396067802118	39.0	35.0	41.0	33.0	41.0
28	37.26815828125822	39.0	35.0	41.0	33.0	41.0
29	37.43681332630485	40.0	35.0	41.0	33.0	41.0
30	37.128819777494876	39.0	35.0	41.0	33.0	41.0
31	37.008111992954284	39.0	35.0	41.0	32.0	41.0
32	36.898525771987174	39.0	35.0	41.0	32.0	41.0
33	36.66789849807655	40.0	35.0	41.0	31.0	41.0
34	36.346219711579735	40.0	35.0	41.0	30.0	41.0
35	36.214225114035955	40.0	35.0	41.0	29.0	41.0
36	35.96136394479007	39.0	35.0	41.0	27.0	41.0
37	35.931247928612244	39.0	35.0	41.0	27.0	41.0
38	35.76553229241579	39.0	35.0	41.0	26.0	41.0
39	35.67327602840074	39.0	35.0	41.0	25.0	41.0
40	35.59922397390721	39.0	35.0	41.0	25.0	41.0
41	35.41553769302564	39.0	35.0	41.0	24.0	41.0
42	35.54160546744817	39.0	35.0	41.0	24.0	41.0
43	35.450502256716376	39.0	35.0	41.0	23.0	41.0
44	35.49956472007762	39.0	35.0	41.0	24.0	41.0
45	35.49927761073327	39.0	35.0	41.0	23.0	41.0
46	35.400229502839245	39.0	35.0	41.0	23.0	41.0
47	35.37910804081569	39.0	35.0	41.0	23.0	41.0
48	35.33134264700045	39.0	35.0	41.0	23.0	41.0
49	35.27943309290618	39.0	35.0	41.0	24.0	41.0
50	35.211081682146876	39.0	35.0	41.0	24.0	41.0
51	34.3905693350603	38.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	24.0
9	52.0
10	70.0
11	78.0
12	59.0
13	54.0
14	89.0
15	89.0
16	156.0
17	242.0
18	453.0
19	810.0
20	1354.0
21	2146.0
22	3428.0
23	5071.0
24	8168.0
25	13357.0
26	18830.0
27	21161.0
28	20043.0
29	18384.0
30	18506.0
31	20909.0
32	25876.0
33	36496.0
34	61929.0
35	74678.0
36	84962.0
37	121666.0
38	214227.0
39	309759.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.36355243807532	20.024168883070796	23.033554866041797	20.578723812812093
2	38.819860581179476	22.712010863996028	23.483698005282445	14.984430549542054
3	28.806483686003926	21.564312031543253	34.817131657636416	14.812072624816402
4	26.292938310264574	23.5348422421855	33.764335849617474	16.407883597932443
5	22.845595179517193	31.360925987642297	30.001449394439312	15.792029438401197
6	24.752241253089196	30.668355472756463	31.196599739108997	13.382803535045342
7	74.49702781821824	5.010473490391068	16.508141073161184	3.9843576182295046
8	76.52710321442451	4.843839288928935	15.357118788490883	3.27193870815566
9	70.60360354538497	7.351107032701846	17.174031652189647	4.8712577697235355
10	34.59787612939677	31.756509119645205	22.85436540064678	10.79124935031125
11	25.207554206890443	23.68929045218337	35.24133340595692	15.861821934969273
12	24.615148849116192	21.17629898514694	36.76578247451327	17.442769691223596
13	23.07694438110396	21.40727891426509	37.366681099065644	18.149095605565304
14	18.340655698658896	24.458669640540947	37.40120807488107	19.799466585919088
15	17.867156075778404	26.132674058886035	38.502193940054156	17.49797592528141
16	22.146931668899224	25.257498308270502	36.08410549745156	16.51146452537871
17	21.69632693907281	25.614584785420387	34.46872308349897	18.220365192007836
18	21.214241731297044	25.79562061315847	35.31777280696004	17.67236484858444
19	21.570405027275388	26.6002653222687	34.23017306877423	17.599156581681687
20	23.000689616335137	26.533611641683848	34.4668767211559	15.99882202082512
21	22.635294508641437	25.801528972656296	35.38738066729382	16.175795851408452
22	21.493411717569337	23.702307306702018	36.35496685318004	18.449314122548607
23	20.451601765491674	25.08052447768717	36.67835721756887	17.78951653925228
24	20.538103841264537	24.819079569908357	35.97729343590492	18.66552315292219
25	20.726248164023445	26.932887498372892	33.61690381652328	18.72396052108038
26	19.515219103203346	27.009603853727484	34.438996649775525	19.03618039329364
27	19.765585836923737	27.45614658639914	34.85775162918397	17.920515947493147
28	19.263006007139882	26.676058496451756	35.39005789269127	18.670877603717095
29	19.954191750268414	25.85110380156775	35.58447984741662	18.610224600747223
30	21.395000604683666	24.952940839780986	35.20366761415828	18.44839094137707
31	21.50412061915915	25.826270228053446	33.91804551467812	18.75156363810929
32	22.417239115924783	26.394118966664852	33.17950057744982	18.009141339960543
33	22.516481091864833	26.289614858047045	32.25973517624913	18.93416887383898
34	21.135032786779306	25.915080256755147	33.97214393133009	18.977743025135453
35	21.78578319459459	26.686398125572946	32.435508871309466	19.09230980852299
36	22.43302551395804	27.619641971878057	31.281624725007408	18.6657077891565
37	22.495432561153827	28.136346473586404	31.253375381158428	18.114845584101342
38	21.865638365932398	27.867331480200995	30.43008241238318	19.836947741483424
39	21.80221581944792	26.164062218718236	31.378097157432855	20.65562480440099
40	22.8275008285551	25.452474171698775	32.400704941142585	19.31932005860354
41	20.925009070255012	26.526041556077256	32.22548515478517	20.323464218882563
42	21.81707903630964	27.044130829542905	31.39157560253727	19.747214531610187
43	21.606132138613805	27.251661956904055	31.23288075915034	19.909325145331795
44	21.732238686645537	27.057147684061555	30.447530536525203	20.763083092767705
45	20.956581866321518	26.13230478641742	30.851699253423387	22.059414093837674
46	20.81302719414777	27.333548126819245	30.934693240744416	20.91873143828857
47	20.60586533925523	26.413782725618557	32.71274017712154	20.267611758004673
48	20.93359465515029	25.87907619106527	32.48305270164354	20.704276452140906
49	20.68175083155544	25.95985454357461	32.6333465963695	20.72504802850045
50	19.5792878765079	26.232746897880467	32.791118258584895	21.39684696702674
51	19.775094602990553	25.5575321890195	31.850858235376116	22.81651497261383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	421.0
1	951.5
2	1482.0
3	19428.5
4	37375.0
5	24216.0
6	11057.0
7	10607.0
8	10157.0
9	10075.5
10	9994.0
11	9740.5
12	9487.0
13	9114.0
14	8741.0
15	8291.5
16	7842.0
17	7674.0
18	7506.0
19	7430.5
20	7355.0
21	7460.5
22	7566.0
23	8044.5
24	8523.0
25	9897.0
26	12362.5
27	13454.0
28	16488.5
29	19523.0
30	22298.5
31	25074.0
32	27543.5
33	30013.0
34	33839.5
35	37666.0
36	40425.0
37	43184.0
38	46373.5
39	49563.0
40	51837.0
41	54111.0
42	56986.5
43	59862.0
44	61250.5
45	62639.0
46	64783.0
47	66927.0
48	73923.5
49	80920.0
50	79968.0
51	79016.0
52	76196.0
53	73376.0
54	66098.5
55	58821.0
56	52568.0
57	46315.0
58	42902.0
59	39489.0
60	36736.5
61	33984.0
62	30617.0
63	27250.0
64	22443.5
65	17637.0
66	13909.5
67	10182.0
68	8122.5
69	6063.0
70	5117.0
71	4171.0
72	3252.5
73	2334.0
74	1856.5
75	1067.5
76	756.0
77	600.5
78	445.0
79	305.5
80	166.0
81	130.0
82	94.0
83	51.0
84	8.0
85	6.5
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1083211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.868659524937314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0256218580602	24.988602800242134
2	7.9359511448297475	5.21688152371891
3	3.0577153929054917	3.0150901852071157
4	1.7305163212033088	2.275190070559145
5	1.195813564751581	1.9652394457560645
6	0.8727497911971502	1.7211669442391557
7	0.7405896221962303	1.7039531697768895
8	0.6510136156362304	1.711835590275653
9	0.5735589460370086	1.69669023432951
>10	7.071861077047796	48.23099137990934
>50	0.1155178590926101	2.2929068061905498
>100	0.02315967162747648	1.2499910269089471
>500	0.0031067852174514925	0.7140683978664355
>1k	0.002541915177914858	1.077508046546505
>5k	0.0	0.0
>10k+	2.8243501976831756E-4	2.1398843784736354
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23051	2.128024918506182	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	1968	0.1816820545581609	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCC	1731	0.15980266079277258	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT	1397	0.1289684096634912	No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	1230	0.11355128409885054	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	1111	0.10256542815757966	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.231811715353703E-5	0.0	0.0	0.08336325978964394	0.0
2	9.231811715353703E-5	0.0	0.0	0.36816465120830566	0.0
3	9.231811715353703E-5	0.0	0.0	0.5614787885278122	0.0
4	9.231811715353703E-5	0.0	0.0	0.8184924266832593	0.0
5	9.231811715353703E-5	0.0	0.0	1.517248255418381	0.0
6	9.231811715353703E-5	0.0	0.0	2.191447464990662	0.0
7	9.231811715353703E-5	0.0	0.0	2.7065825587073986	0.0
8	9.231811715353703E-5	0.0	0.0	3.361764236146051	0.0
9	9.231811715353703E-5	0.0	0.0	3.7439612411616943	0.0
10	9.231811715353703E-5	0.0	0.0	4.44437879600558	0.0
11	9.231811715353703E-5	0.0	0.0	5.222343569258436	0.0
12	9.231811715353703E-5	0.0	0.0	5.865339255232822	0.0
13	9.231811715353703E-5	0.0	0.0	6.165557772216124	0.0
14	9.231811715353703E-5	0.0	0.0	6.350009370288891	0.0
15	9.231811715353703E-5	0.0	0.0	6.528367972629525	0.0
16	0.0015694079916101296	0.0	0.0	6.810307502416427	0.0
17	0.0015694079916101296	0.0	0.0	7.1492073104870615	0.0
18	0.0015694079916101296	0.0	0.0	7.520418459561434	0.0
19	0.0015694079916101296	0.0	0.0	7.785648410143545	0.0
20	0.0015694079916101296	0.0	0.0	8.034445735872328	0.0
21	0.0015694079916101296	0.0	0.0	8.333833389801248	0.0
22	0.0015694079916101296	0.0	0.0	8.662855159336454	0.0
23	0.0015694079916101296	0.0	0.0	8.96759726406028	0.0
24	0.0015694079916101296	0.0	0.0	9.214178954977378	0.0
25	0.0015694079916101296	0.0	0.0	9.448297700078747	0.0
26	0.0015694079916101296	0.0	0.0	9.673369269699071	0.0
27	0.0015694079916101296	0.0	0.0	9.911826966306657	0.0
28	0.0015694079916101296	0.0	0.0	10.144560939650724	0.0
29	0.0015694079916101296	0.0	0.0	10.397512580651416	0.0
30	0.0015694079916101296	0.0	0.0	10.709455498513217	0.0
31	0.0016617261087636666	0.0	0.0	10.985578986919446	0.0
32	0.0016617261087636666	0.0	0.0	11.265579836246124	0.0
33	0.0016617261087636666	0.0	0.0	11.553150771179391	0.0
34	0.0016617261087636666	0.0	0.0	11.840629387995506	0.0
35	0.0016617261087636666	0.0	0.0	12.124692234476939	0.0
36	0.0016617261087636666	0.0	0.0	12.393153319159426	0.0
37	0.0016617261087636666	0.0	0.0	12.671030851791572	0.0
38	0.0016617261087636666	0.0	0.0	12.98158899789607	0.0
39	0.0016617261087636666	0.0	0.0	13.39942079613298	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	20	7.0331054E-4	45.000004	2
TGCGTCG	20	7.0331054E-4	45.000004	2
CAATGCG	30	2.1653868E-6	44.999996	1
CGTTTTT	10300	0.0	43.82039	1
CGTTATT	690	0.0	42.06522	1
ACACGAC	50	1.0822987E-9	40.500004	26
TATTGCG	45	1.9281288E-8	40.000004	1
TATAGCG	45	1.9281288E-8	40.000004	1
CGAATGG	130	0.0	39.80769	2
CGTAAGG	165	0.0	39.545452	2
TACGTTG	40	3.4579716E-7	39.375004	1
CGAATAT	120	0.0	39.374996	14
GCTACGA	120	0.0	39.374996	10
CCGAATT	35	6.2479576E-6	38.571426	29
AATGCGG	125	0.0	37.8	2
CGGCATG	30	1.1398787E-4	37.499996	1
GCCGATT	60	1.5643309E-10	37.499996	9
TGCTACG	85	0.0	37.058823	1
AAGGGCG	310	0.0	37.01613	5
GCGCGAC	55	2.748493E-9	36.818184	9
>>END_MODULE