FastQCFastQC Report
Sat 18 Jun 2016
SRR3551363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210726
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG124941.0319428177804062No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC119450.9865981237703658No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC103620.8558501262878637No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC73700.608725673686697TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46920.38753607339728396No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC35080.289743509266341TruSeq Adapter, Index 16 (100% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT29130.24059944198769995No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT28400.23457000180057255TruSeq Adapter, Index 16 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT25680.21210414247319376No Hit
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT23590.194841772622377TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC21460.17724902248733404No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC19820.16370343083406155No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTA18230.15057081453607174No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17570.14511953984634013No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC16900.13958567008555198TruSeq Adapter, Index 16 (100% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT16090.13289546932997226No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14070.11621126497655125No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA13710.11323784241851584No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC13480.11133815578421542No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG207.0334366E-445.019
TTAGCCG253.8909297E-545.042
CGCATCG207.0334366E-445.021
CGTCTAA351.2119926E-745.019
TCTAGCG253.8909297E-545.01
CTCGACG351.2119926E-745.01
CGGTCTA406.8157533E-945.031
ACGTACG453.8562575E-1045.01
ATCGGTA207.0334366E-445.032
GCGCATC302.1655997E-644.99999621
GTTACCG302.1655997E-644.99999638
CTAACGG1000.042.752
CTCGAAT1900.042.63157743
ACGGGTC700.041.7857135
TAACGGG3300.040.909093
GGTTACG501.0822987E-940.51
ACCGGTA451.9284926E-840.041
CGTTGAT900.040.025
CGAATAT3050.039.83606714
CCGATGA14150.039.5936418
Produced by FastQC (version 0.11.5)