##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1210726
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.528277248526916	31.0	31.0	34.0	30.0	34.0
2	31.66749867434911	31.0	31.0	34.0	30.0	34.0
3	31.64341312567831	31.0	31.0	34.0	30.0	34.0
4	35.37870170459708	37.0	35.0	37.0	33.0	37.0
5	33.34356906517247	37.0	35.0	37.0	30.0	37.0
6	34.24956926670444	37.0	35.0	37.0	30.0	37.0
7	35.33266073413803	37.0	35.0	37.0	32.0	37.0
8	35.12174265688521	37.0	35.0	37.0	32.0	37.0
9	36.86633557055849	39.0	37.0	39.0	32.0	39.0
10	36.50786965837027	38.0	35.0	39.0	32.0	39.0
11	36.69176510622552	39.0	35.0	39.0	32.0	39.0
12	36.709018390618525	39.0	35.0	39.0	33.0	39.0
13	36.79002268060651	39.0	35.0	39.0	33.0	39.0
14	37.790200260009286	39.0	37.0	41.0	33.0	41.0
15	37.86545841090387	40.0	37.0	41.0	33.0	41.0
16	37.957380943334826	40.0	37.0	41.0	33.0	41.0
17	37.77892768471149	39.0	36.0	41.0	33.0	41.0
18	37.78619357311233	39.0	37.0	41.0	33.0	41.0
19	37.71747612589471	39.0	37.0	41.0	33.0	41.0
20	37.800328893572946	39.0	36.0	41.0	33.0	41.0
21	37.74745648478682	39.0	36.0	41.0	33.0	41.0
22	37.894654116620934	40.0	37.0	41.0	33.0	41.0
23	37.923137026874784	40.0	36.0	41.0	33.0	41.0
24	37.77285033938315	40.0	36.0	41.0	33.0	41.0
25	37.53049988188905	39.0	36.0	41.0	33.0	41.0
26	37.59707233511133	39.0	36.0	41.0	33.0	41.0
27	37.21666917205049	39.0	36.0	41.0	32.0	41.0
28	37.23352104439815	39.0	36.0	41.0	32.0	41.0
29	37.498207686958075	40.0	36.0	41.0	32.0	41.0
30	37.23122242357065	39.0	36.0	41.0	32.0	41.0
31	37.177248196536624	39.0	35.0	41.0	32.0	41.0
32	37.25409134684479	39.0	36.0	41.0	32.0	41.0
33	37.267688147442115	40.0	36.0	41.0	32.0	41.0
34	37.2230802014659	40.0	36.0	41.0	32.0	41.0
35	37.17955755472337	40.0	36.0	41.0	32.0	41.0
36	36.97698323154867	40.0	35.0	41.0	31.0	41.0
37	36.9789787284654	40.0	35.0	41.0	31.0	41.0
38	36.81843290719783	39.0	35.0	41.0	31.0	41.0
39	36.75021350825868	39.0	35.0	41.0	31.0	41.0
40	36.704432712273466	39.0	35.0	41.0	31.0	41.0
41	36.604792496402986	39.0	35.0	41.0	30.0	41.0
42	36.7038966702623	39.0	35.0	41.0	31.0	41.0
43	36.63483562754909	39.0	35.0	41.0	31.0	41.0
44	36.74322018359232	39.0	35.0	41.0	31.0	41.0
45	36.710946159576984	39.0	35.0	41.0	31.0	41.0
46	36.545030832740025	39.0	35.0	41.0	31.0	41.0
47	36.44695166371252	39.0	35.0	41.0	31.0	41.0
48	36.46753931112407	39.0	35.0	41.0	31.0	41.0
49	36.47053916410484	39.0	35.0	41.0	31.0	41.0
50	36.37420770678089	39.0	35.0	41.0	31.0	41.0
51	35.449797889861124	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	19.0
9	43.0
10	61.0
11	51.0
12	43.0
13	51.0
14	73.0
15	84.0
16	111.0
17	193.0
18	372.0
19	653.0
20	1311.0
21	2304.0
22	3732.0
23	5404.0
24	6904.0
25	8694.0
26	10300.0
27	11570.0
28	13088.0
29	15045.0
30	19016.0
31	25231.0
32	33418.0
33	46857.0
34	78019.0
35	93261.0
36	103943.0
37	152742.0
38	252556.0
39	325480.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.375606041333874	20.875821614469334	22.586200345908157	12.16237199828863
2	29.60925923784572	29.278383383193223	23.581470952139462	17.530886426821592
3	30.101195481058475	27.92060301009477	26.256229733234438	15.721971775612317
4	28.586236687739426	23.632019135626063	30.16950160482223	17.612242571812285
5	26.83976391024889	32.81551730118954	22.21716556842754	18.127553220134036
6	24.8822607262089	36.43004280076582	24.094964508897966	14.592731964127308
7	73.29412269993377	6.438285788857264	14.716624570712117	5.550966940496859
8	72.50178818328837	10.496016439722942	10.744462413461015	6.257732963527668
9	66.09241066930089	8.897801814778902	12.983697384874858	12.02609013104534
10	39.64769898391543	25.26987939467724	20.432120892753606	14.650300728653717
11	29.849032729122854	24.231494161354426	26.087735788279097	19.83173732124362
12	26.606019859158884	20.69692069056087	30.14174966094723	22.555309789333013
13	24.90860855387594	23.29048851680727	32.22620146920113	19.574701460115666
14	19.011733455794293	28.74341510796002	29.001689895153817	23.243161541091876
15	18.303645911626578	25.587127062605415	34.51879285651749	21.590434169250518
16	20.941319505817173	26.535401073405545	28.41138292231273	24.111896498464557
17	20.381490114195948	28.709138153471553	28.57128697987819	22.338084752454314
18	20.22976296866508	26.332877959174912	30.010340902896278	23.42701816926373
19	21.481573865597998	27.116292208146188	26.998676826961677	24.403457099294144
20	23.442050472196023	26.73858494820463	30.92359460356844	18.89576997603091
21	22.873135622758575	29.273592869071948	28.57095659959396	19.282314908575515
22	21.028787686066046	24.62340777351771	29.18282088598081	25.164983654435435
23	21.617112377201778	26.5803327920603	29.49734291656411	22.30521191417381
24	21.946997091001595	25.09865981237704	29.69796634416045	23.25637675246092
25	19.752611243171454	30.79548964835974	26.37467106512952	23.077228043339286
26	18.82349928885644	26.528297897294685	29.869185926460652	24.779016887388227
27	22.389541481722535	27.857252590594406	28.140553684318338	21.612652243364725
28	17.729362382570457	28.412043682881183	30.32981863774297	23.52877529680539
29	20.18161004223912	24.085631265868578	28.712111576029585	27.02064711586271
30	19.60327935470123	27.90870931986263	29.71068598510315	22.77732534033299
31	20.85855924461852	27.59377431392404	24.881765155782563	26.665901285674877
32	21.824838980908975	26.49013897446656	26.331308652824838	25.35371339179963
33	20.171285658357053	25.327200373990483	26.023889798352396	28.477624169300075
34	19.28768358819419	24.621260301670237	26.781369195011916	29.30968691512365
35	19.695372858929268	23.178407005383548	29.393851292530265	27.73236884315692
36	18.502947818086007	27.898797911335844	26.376157776408537	27.222096494169612
37	18.6310527732947	25.804186909341997	30.896751205475066	24.66800911188824
38	18.13490418145807	27.482931728566168	25.92708837507413	28.45507571490164
39	20.420640177876745	23.850317908428497	27.15387296547691	28.575168948217843
40	19.734440327539012	22.76394493882183	30.536471505526436	26.96514322811272
41	18.317026313137738	24.503397135272557	27.18096414878346	29.998612402806252
42	18.56679380801271	23.967933289613008	31.57617825998616	25.889094642388123
43	21.05736558065161	23.610544417151363	27.33442579080651	27.99766421139052
44	19.750628961466095	24.237275816328385	28.217449695471974	27.794645526733547
45	19.6187246329888	23.61442638549102	27.3842306186536	29.382618362866573
46	21.837889002135906	25.972680854297337	26.688036764718028	25.501393378848725
47	17.664938227146358	23.847757461225743	33.31158329795511	25.175721013672785
48	19.311966539084814	24.097029385674382	29.29300271077023	27.298001364470576
49	19.415953733544995	22.393340854991138	32.47621674928927	25.714488662174595
50	19.539598554916637	22.158853448261624	30.235660256738516	28.065887740083223
51	19.153301407585204	21.507343527767635	28.011622778399076	31.327732286248082
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	521.0
1	1297.5
2	2074.0
3	5235.0
4	8396.0
5	5635.5
6	2875.0
7	2850.0
8	2825.0
9	2789.5
10	2754.0
11	2760.0
12	2766.0
13	2707.5
14	2649.0
15	2791.5
16	2934.0
17	2874.5
18	2815.0
19	2870.0
20	2925.0
21	3853.0
22	4781.0
23	4921.0
24	5061.0
25	6180.5
26	9531.5
27	11763.0
28	14312.5
29	16862.0
30	20215.5
31	23569.0
32	25154.0
33	26739.0
34	29315.5
35	31892.0
36	33897.5
37	35903.0
38	38952.5
39	42002.0
40	47343.5
41	52685.0
42	56933.0
43	61181.0
44	64748.0
45	68315.0
46	74501.5
47	80688.0
48	112827.5
49	144967.0
50	134470.0
51	123973.0
52	119009.5
53	114046.0
54	95666.5
55	77287.0
56	68219.0
57	59151.0
58	53505.5
59	47860.0
60	43883.0
61	39906.0
62	36660.0
63	33414.0
64	27823.5
65	22233.0
66	19286.0
67	16339.0
68	14110.5
69	11882.0
70	9590.0
71	7298.0
72	5994.5
73	4691.0
74	3707.0
75	2073.0
76	1423.0
77	1101.0
78	779.0
79	525.0
80	271.0
81	225.0
82	179.0
83	96.0
84	13.0
85	9.0
86	5.0
87	3.0
88	1.0
89	3.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1210726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.672785858655807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5653918341861	19.355211571426963
2	11.274296997208321	6.014338190268475
3	4.555435379458294	3.645184571077066
4	2.3524190747870595	2.5098228092644983
5	1.3956380696034247	1.861277768335996
6	0.881337960064579	1.4104643206744234
7	0.6240422397418741	1.165146151917367
8	0.44666716215062674	0.9531086052909729
9	0.3378947106879858	0.8111333934857794
>10	4.1668562292768545	27.301168350681426
>50	1.27881066535329	22.697185161250285
>100	0.10962115534521105	4.8761489317869495
>500	0.005011252815741742	0.843420302130931
>1k	0.0053244561167256005	3.0334226020875823
>5k	3.132033009838589E-4	0.6156901373519177
>10k+	9.396099029515767E-4	2.907277132969347
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG	12494	1.0319428177804062	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	11945	0.9865981237703658	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC	10362	0.8558501262878637	No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	7370	0.608725673686697	TruSeq Adapter, Index 16 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4692	0.38753607339728396	No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	3508	0.289743509266341	TruSeq Adapter, Index 16 (100% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT	2913	0.24059944198769995	No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	2840	0.23457000180057255	TruSeq Adapter, Index 16 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT	2568	0.21210414247319376	No Hit
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT	2359	0.194841772622377	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC	2146	0.17724902248733404	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1982	0.16370343083406155	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTA	1823	0.15057081453607174	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1757	0.14511953984634013	No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	1690	0.13958567008555198	TruSeq Adapter, Index 16 (100% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1609	0.13289546932997226	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1407	0.11621126497655125	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	1371	0.11323784241851584	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC	1348	0.11133815578421542	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6519014211307926E-4	0.0	0.0	0.24720704767222312	0.0
2	1.6519014211307926E-4	0.0	0.0	1.4562336977978503	0.0
3	1.6519014211307926E-4	0.0	0.0	1.8198997956597942	0.0
4	1.6519014211307926E-4	0.0	0.0	2.4606723569164286	0.0
5	1.6519014211307926E-4	0.0	0.0	4.795222040329521	0.0
6	1.6519014211307926E-4	0.0	0.0	5.6576797723019085	0.0
7	1.6519014211307926E-4	0.0	0.0	6.751651488445775	0.0
8	1.6519014211307926E-4	0.0	0.0	8.193100668524504	0.0
9	1.6519014211307926E-4	0.0	0.0	8.657119777720144	0.0
10	1.6519014211307926E-4	0.0	0.0	10.793689075810711	0.0
11	1.6519014211307926E-4	0.0	0.0	12.27337977378862	0.0
12	1.6519014211307926E-4	0.0	0.0	14.337430599491544	0.0
13	1.6519014211307926E-4	0.0	0.0	14.851502321747448	0.0
14	1.6519014211307926E-4	0.0	0.0	15.080373263645118	0.0
15	1.6519014211307926E-4	0.0	0.0	15.617654200867909	0.0
16	1.6519014211307926E-4	0.0	0.0	16.36257914672684	0.0
17	1.6519014211307926E-4	0.0	0.0	17.22685397026247	0.0
18	1.6519014211307926E-4	0.0	0.0	18.130691832834184	0.0
19	1.6519014211307926E-4	0.0	0.0	18.940701694685668	0.0
20	2.477852131696189E-4	0.0	0.0	19.591963829966485	0.0
21	2.477852131696189E-4	0.0	0.0	20.276098803527802	0.0
22	2.477852131696189E-4	0.0	0.0	21.043902584069393	0.0
23	2.477852131696189E-4	0.0	0.0	21.716143867398568	0.0
24	2.477852131696189E-4	0.0	0.0	22.28084636821213	0.0
25	3.303802842261585E-4	0.0	0.0	22.803012407431574	0.0
26	4.955704263392378E-4	0.0	0.0	23.269839749043136	0.0
27	4.955704263392378E-4	0.0	0.0	23.76334529860596	0.0
28	4.955704263392378E-4	0.0	0.0	24.21423179150361	0.0
29	6.60760568452317E-4	0.0	0.0	24.718722485516956	0.0
30	6.60760568452317E-4	0.0	0.0	25.263684764348003	0.0
31	6.60760568452317E-4	0.0	0.0	25.767432102721838	0.0
32	6.60760568452317E-4	0.0	0.0	26.254908212097536	0.0
33	6.60760568452317E-4	0.0	0.0	26.698113363386927	0.0
34	6.60760568452317E-4	0.0	0.0	27.18591985304685	0.0
35	6.60760568452317E-4	0.0	0.0	27.68173806459926	0.0
36	6.60760568452317E-4	0.0	0.0	28.145591983652782	0.0
37	7.433556395088567E-4	0.0	0.0	28.604490198442917	0.0
38	7.433556395088567E-4	0.0	0.0	29.06148872659875	0.0
39	7.433556395088567E-4	0.0	0.0	29.528811638636654	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	20	7.0334366E-4	45.0	19
TTAGCCG	25	3.8909297E-5	45.0	42
CGCATCG	20	7.0334366E-4	45.0	21
CGTCTAA	35	1.2119926E-7	45.0	19
TCTAGCG	25	3.8909297E-5	45.0	1
CTCGACG	35	1.2119926E-7	45.0	1
CGGTCTA	40	6.8157533E-9	45.0	31
ACGTACG	45	3.8562575E-10	45.0	1
ATCGGTA	20	7.0334366E-4	45.0	32
GCGCATC	30	2.1655997E-6	44.999996	21
GTTACCG	30	2.1655997E-6	44.999996	38
CTAACGG	100	0.0	42.75	2
CTCGAAT	190	0.0	42.631577	43
ACGGGTC	70	0.0	41.785713	5
TAACGGG	330	0.0	40.90909	3
GGTTACG	50	1.0822987E-9	40.5	1
ACCGGTA	45	1.9284926E-8	40.0	41
CGTTGAT	90	0.0	40.0	25
CGAATAT	305	0.0	39.836067	14
CCGATGA	1415	0.0	39.59364	18
>>END_MODULE