FastQCFastQC Report
Sat 18 Jun 2016
SRR3551362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164149
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.45397968816706447No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC46840.4023539942052091TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT37560.32263911234730264TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC33900.29119983782144726TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC22530.1935319276140769No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC22340.191899834127762TruSeq Adapter, Index 19 (95% over 21bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC17540.15066799868401726No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15690.13477656210674063No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC14050.12068901833012784TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTC253.8908358E-545.00000414
ACGATAG351.211938E-745.0000041
GGGCGTA253.8908358E-545.0000047
TCGAGTG253.8908358E-545.0000041
TAGCGTA207.033324E-445.041
CTATACG207.033324E-445.01
TAACGCC406.8157533E-945.012
GCTTCGT207.033324E-445.024
CGTAGCG406.8157533E-945.02
TACGACG207.033324E-445.01
CGGTGAT207.033324E-445.014
ATGGTCG207.033324E-445.044
CGACTCG207.033324E-445.022
TCGGTAT207.033324E-445.01
AATCGGC207.033324E-445.037
GCTACCG302.165527E-644.9999961
ACGGTGC302.165527E-644.99999626
CGTAAGG2350.044.0425532
CGTTATT1550.043.5483861
TATTGCG1100.042.9545441
Produced by FastQC (version 0.11.5)