##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1164149
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.620945428806795	31.0	31.0	34.0	30.0	34.0
2	31.763797417684508	33.0	31.0	34.0	30.0	34.0
3	31.73022525467101	33.0	31.0	34.0	30.0	34.0
4	35.414747596742345	37.0	35.0	37.0	33.0	37.0
5	33.311866436341056	37.0	35.0	37.0	30.0	37.0
6	34.30276708565656	37.0	35.0	37.0	30.0	37.0
7	35.479686019573094	37.0	35.0	37.0	32.0	37.0
8	35.4823428959695	37.0	35.0	37.0	33.0	37.0
9	37.45497784218343	39.0	37.0	39.0	34.0	39.0
10	36.81614724575634	39.0	37.0	39.0	32.0	39.0
11	36.85503230256608	39.0	37.0	39.0	33.0	39.0
12	36.95655624838401	39.0	37.0	39.0	33.0	39.0
13	37.14162276478355	39.0	37.0	39.0	34.0	39.0
14	38.19859657140108	40.0	38.0	41.0	34.0	41.0
15	38.289784211471215	40.0	38.0	41.0	34.0	41.0
16	38.29959738830682	40.0	38.0	41.0	34.0	41.0
17	38.21549990593987	40.0	37.0	41.0	34.0	41.0
18	38.18316641598283	40.0	37.0	41.0	34.0	41.0
19	38.06753173348085	40.0	37.0	41.0	34.0	41.0
20	38.28154213936532	40.0	37.0	41.0	34.0	41.0
21	38.16191913578073	40.0	37.0	41.0	34.0	41.0
22	38.349061846894166	40.0	37.0	41.0	34.0	41.0
23	38.42715236623491	40.0	38.0	41.0	34.0	41.0
24	38.28522809365468	40.0	37.0	41.0	34.0	41.0
25	37.96883130939425	40.0	37.0	41.0	34.0	41.0
26	38.10141657124647	40.0	37.0	41.0	34.0	41.0
27	37.72633571819415	40.0	37.0	41.0	34.0	41.0
28	37.81161432084725	40.0	37.0	41.0	33.0	41.0
29	38.06074823755378	40.0	37.0	41.0	34.0	41.0
30	37.77548750202938	40.0	37.0	41.0	33.0	41.0
31	37.68660970374067	40.0	37.0	41.0	33.0	41.0
32	37.79119339534716	40.0	37.0	41.0	33.0	41.0
33	37.819329828054656	40.0	37.0	41.0	34.0	41.0
34	37.72957413526963	40.0	37.0	41.0	33.0	41.0
35	37.72199950349998	40.0	37.0	41.0	33.0	41.0
36	37.53205904055237	40.0	37.0	41.0	33.0	41.0
37	37.51207190832101	40.0	37.0	41.0	33.0	41.0
38	37.341866032612664	40.0	36.0	41.0	33.0	41.0
39	37.335554984800055	40.0	36.0	41.0	33.0	41.0
40	37.2907239537207	40.0	36.0	41.0	33.0	41.0
41	37.18513609512184	40.0	36.0	41.0	32.0	41.0
42	37.38240637581615	40.0	37.0	41.0	33.0	41.0
43	37.28592989385379	40.0	36.0	41.0	33.0	41.0
44	37.344560704858225	40.0	36.0	41.0	33.0	41.0
45	37.32424371794332	40.0	36.0	41.0	33.0	41.0
46	37.202901003222095	40.0	36.0	41.0	32.0	41.0
47	37.17935676618715	40.0	36.0	41.0	32.0	41.0
48	37.16773969655087	40.0	36.0	41.0	32.0	41.0
49	37.16152571535087	40.0	36.0	41.0	32.0	41.0
50	37.11789384348567	39.0	36.0	41.0	32.0	41.0
51	36.24786947375293	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	36.0
10	28.0
11	39.0
12	26.0
13	32.0
14	43.0
15	50.0
16	93.0
17	138.0
18	253.0
19	405.0
20	668.0
21	1098.0
22	1667.0
23	2721.0
24	4074.0
25	6140.0
26	8447.0
27	9924.0
28	10456.0
29	11828.0
30	14822.0
31	19459.0
32	26111.0
33	37289.0
34	61781.0
35	79848.0
36	96261.0
37	145555.0
38	260426.0
39	364295.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.65948430999812	23.14695112051808	26.049071038157486	12.144493531326317
2	30.487592224019433	24.407614489210573	26.97695913495609	18.1278341518139
3	31.812164937649733	23.088453453982265	28.93641621476289	16.162965393605113
4	27.818002678351313	25.928639718798884	27.773420756277762	18.479936846572045
5	23.328199397156204	34.175865804119574	25.237834675801807	17.25810012292241
6	25.36118658350435	34.19974590881408	25.37286893688007	15.066198570801504
7	80.34529944190993	5.14160987983497	9.699531589169426	4.813559089085675
8	81.64719464604617	4.97436324731628	8.706703351546924	4.671738755090629
9	75.41526041769568	8.268185601671265	11.072379910131778	5.244174070501285
10	34.45048700810635	36.96202118457345	17.70220135051441	10.885290456805787
11	23.061566861286657	25.55557750769017	32.4881952396128	18.89466039141038
12	23.287311160341158	22.81941572771183	33.91971302642531	19.9735600855217
13	21.643707119964883	24.944487346551	34.16220775862884	19.249597774855282
14	18.405633643116133	28.138923797555126	31.999168491318553	21.456274068010195
15	17.012856601689304	28.922929968586498	34.751221707874166	19.312991721850036
16	19.723420283829647	28.212282104782123	31.71389572984214	20.35040188154609
17	20.166318916221204	28.182904422028454	29.445801181807486	22.20497547994286
18	20.167092013135775	27.59784185701315	32.39834419820831	19.83672193164277
19	20.475299983077768	28.357538425064142	30.493175701735776	20.673985890122314
20	21.91600903320795	28.755082038467584	31.241018117096697	18.087890811227773
21	21.176155285964253	28.142875181785147	31.779265368951915	18.901704163298685
22	20.10163647436883	26.41594847394964	30.417240404793546	23.065174646887986
23	18.4185185916923	27.518126975155244	31.52757937343072	22.535775059721736
24	19.551449170166364	27.07368214893454	31.966183022963556	21.40868565793554
25	18.658951732123636	29.877532858766358	29.175990358622478	22.287525050487524
26	18.037983110409407	30.907813346916935	29.527062257494528	21.52714128517913
27	18.327464955087365	30.20549774985848	30.372658482719995	21.09437881233416
28	16.321965659035055	29.94384739410505	31.61219053574757	22.121996411112324
29	17.87795204909337	28.08944559502263	31.1999580809673	22.8326442749167
30	19.26454431520364	28.321804167679566	30.197079583455384	22.216571933661413
31	19.083381938222686	30.510699231799364	27.38309271407698	23.022826115900973
32	18.19672567686782	31.91155084100059	26.72183715314792	23.16988632898366
33	18.40743753591679	28.79536897768241	28.083003120734546	24.71419036566625
34	17.222022266909132	28.46250780613134	29.551887258417953	24.76358266854157
35	17.399748657603105	28.626919749963275	27.731845322205317	26.241486270228297
36	17.818938984614512	29.313172111130104	27.953036939429577	24.914851964825807
37	17.389182999770647	30.025709767392318	28.822341470035195	23.76276576280184
38	16.69519966945812	30.991651412319214	27.90544852935492	24.40770038886775
39	18.888132017465118	27.23251061505014	29.676699460292454	24.202657907192293
40	19.24667718651135	25.548619635459037	30.02347637630578	25.18122680172383
41	16.605176828739275	26.730513018522544	27.718273176371756	28.94603697636643
42	18.10378224780505	27.53187092030316	27.745073869410184	26.619272962481606
43	19.286964125726175	27.640619886286032	28.124578554806988	24.9478374331808
44	18.36800959327371	28.1386660985836	28.114356495603225	25.37896781253946
45	18.900931066384114	27.348389252578492	28.047612461978666	25.703067219058727
46	19.275797170293494	27.746963661868023	28.30075875167182	24.67648041616666
47	16.970250371730767	27.022400053601388	30.519632796145512	25.487716778522334
48	17.05202684536086	26.293713261790373	29.48454192719317	27.1697179656556
49	18.747686077984863	25.14557844399643	30.962101930251194	25.14463354776751
50	18.113574808722937	24.834449885710505	29.919795490096202	27.13217981547036
51	17.16850678048944	24.2662236535014	27.932335122050528	30.632934443958632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	438.0
1	943.5
2	1449.0
3	5242.0
4	9035.0
5	6138.5
6	3242.0
7	3182.5
8	3123.0
9	3187.0
10	3251.0
11	3226.0
12	3201.0
13	3209.5
14	3218.0
15	3148.5
16	3079.0
17	3195.5
18	3312.0
19	3795.5
20	4279.0
21	4194.0
22	4109.0
23	5245.0
24	6381.0
25	7717.5
26	11389.0
27	13724.0
28	16175.0
29	18626.0
30	21959.5
31	25293.0
32	29555.5
33	33818.0
34	38082.5
35	42347.0
36	44724.5
37	47102.0
38	52595.5
39	58089.0
40	63264.0
41	68439.0
42	75239.0
43	82039.0
44	87351.0
45	92663.0
46	105198.5
47	117734.0
48	125702.5
49	133671.0
50	126468.0
51	119265.0
52	98765.0
53	78265.0
54	64534.5
55	50804.0
56	42900.0
57	34996.0
58	30620.5
59	26245.0
60	24388.0
61	22531.0
62	19758.5
63	16986.0
64	14117.5
65	11249.0
66	8206.5
67	5164.0
68	4150.5
69	3137.0
70	2587.0
71	2037.0
72	1649.5
73	1262.0
74	916.5
75	455.0
76	339.0
77	336.5
78	334.0
79	214.0
80	94.0
81	53.5
82	13.0
83	70.5
84	128.0
85	67.5
86	7.0
87	5.5
88	4.0
89	2.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1164149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.10205628140579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74234791008938	23.030768903698842
2	12.1192270429441	7.781042172394531
3	4.817188112371521	4.639249317044084
4	2.4482624865245506	3.143770405362624
5	1.4733518524836742	2.3648812045372196
6	0.9554814663963317	1.8403751886057103
7	0.6494784770444846	1.4594716236550633
8	0.49377053216104255	1.2680839530826782
9	0.3832626077776118	1.1073166024891719
>10	4.082200813148326	31.239501993983648
>50	0.7722460298998476	15.91687991586819
>100	0.05805245243515673	3.2772543914778107
>500	0.002430102658771931	0.5617679839284213
>1k	0.002430102658771931	1.911536699517586
>5k	2.7001140653021456E-4	0.45809964435452966
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5285	0.45397968816706447	No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	4684	0.4023539942052091	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	3756	0.32263911234730264	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	3390	0.29119983782144726	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC	2253	0.1935319276140769	No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	2234	0.191899834127762	TruSeq Adapter, Index 19 (95% over 21bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1754	0.15066799868401726	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1569	0.13477656210674063	No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1405	0.12068901833012784	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7179931434893643E-4	0.0	0.0	0.33715615440978774	0.0
2	1.7179931434893643E-4	0.0	0.0	1.388825657196802	0.0
3	1.7179931434893643E-4	0.0	0.0	1.801229911291424	0.0
4	1.7179931434893643E-4	0.0	0.0	2.332175692286812	0.0
5	2.5769897152340464E-4	0.0	0.0	3.7977097433404143	0.0
6	2.5769897152340464E-4	0.0	0.0	4.668045069832126	0.0
7	2.5769897152340464E-4	0.0	0.0	5.590435588571566	0.0
8	2.5769897152340464E-4	0.0	0.0	7.030886939730223	0.0
9	2.5769897152340464E-4	0.0	0.0	7.574717669301782	0.0
10	3.4359862869787287E-4	0.0	0.0	8.930729657457936	0.0
11	3.4359862869787287E-4	0.0	0.0	10.992321429645175	0.0
12	3.4359862869787287E-4	0.0	0.0	12.648037321683049	0.0
13	3.4359862869787287E-4	0.0	0.0	13.301218314837705	0.0
14	3.4359862869787287E-4	0.0	0.0	13.546891334356685	0.0
15	3.4359862869787287E-4	0.0	0.0	14.002760814981587	0.0
16	3.4359862869787287E-4	0.0	0.0	14.905909810513947	0.0
17	3.4359862869787287E-4	0.0	0.0	16.182722314755242	0.0
18	3.4359862869787287E-4	0.0	0.0	17.495011377409593	0.0
19	3.4359862869787287E-4	0.0	0.0	18.351688658410563	0.0
20	3.4359862869787287E-4	0.0	0.0	19.1452296913883	0.0
21	3.4359862869787287E-4	0.0	0.0	20.118644606489376	0.0
22	3.4359862869787287E-4	0.0	0.0	21.15553936824238	0.0
23	3.4359862869787287E-4	0.0	0.0	22.151631792837513	0.0
24	3.4359862869787287E-4	0.0	0.0	22.926360800894045	0.0
25	3.4359862869787287E-4	0.0	0.0	23.593457538510965	0.0
26	3.4359862869787287E-4	0.0	0.0	24.216315952683033	0.0
27	3.4359862869787287E-4	0.0	0.0	24.85644019794717	0.0
28	3.4359862869787287E-4	0.0	0.0	25.52869091499456	0.0
29	3.4359862869787287E-4	0.0	0.0	26.236504090112177	0.0
30	3.4359862869787287E-4	0.0	0.0	27.017761472113964	0.0
31	3.4359862869787287E-4	0.0	0.0	27.730385028033353	0.0
32	3.4359862869787287E-4	0.0	0.0	28.39241368587698	0.0
33	3.4359862869787287E-4	0.0	0.0	28.99998196107199	0.0
34	3.4359862869787287E-4	0.0	0.0	29.643885791251808	0.0
35	3.4359862869787287E-4	0.0	0.0	30.32309438053033	0.0
36	3.4359862869787287E-4	0.0	0.0	31.002990167066244	0.0
37	3.4359862869787287E-4	0.0	0.0	31.66673681805336	0.0
38	3.4359862869787287E-4	0.0	0.0	32.270525508332696	0.0
39	3.4359862869787287E-4	0.0	0.0	32.903176483422655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTTC	25	3.8908358E-5	45.000004	14
ACGATAG	35	1.211938E-7	45.000004	1
GGGCGTA	25	3.8908358E-5	45.000004	7
TCGAGTG	25	3.8908358E-5	45.000004	1
TAGCGTA	20	7.033324E-4	45.0	41
CTATACG	20	7.033324E-4	45.0	1
TAACGCC	40	6.8157533E-9	45.0	12
GCTTCGT	20	7.033324E-4	45.0	24
CGTAGCG	40	6.8157533E-9	45.0	2
TACGACG	20	7.033324E-4	45.0	1
CGGTGAT	20	7.033324E-4	45.0	14
ATGGTCG	20	7.033324E-4	45.0	44
CGACTCG	20	7.033324E-4	45.0	22
TCGGTAT	20	7.033324E-4	45.0	1
AATCGGC	20	7.033324E-4	45.0	37
GCTACCG	30	2.165527E-6	44.999996	1
ACGGTGC	30	2.165527E-6	44.999996	26
CGTAAGG	235	0.0	44.042553	2
CGTTATT	155	0.0	43.548386	1
TATTGCG	110	0.0	42.954544	1
>>END_MODULE