FastQCFastQC Report
Sat 18 Jun 2016
SRR3551361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220977
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84510.6921506301920511No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC66770.546857148005245No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG53550.43858320017494185No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC48220.39492963421915406No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC29830.2443125464279835TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT15900.13022358324522085TruSeq Adapter, Index 16 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT15410.12621040363577693No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT15390.12604660038641186No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC14600.11957637203649207No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14070.11523558592831805No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC13860.11351565180998495TruSeq Adapter, Index 13 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13020.10663591533665254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG650.045.0000041
CGATCGG207.03346E-445.010
GCGATCG253.89095E-544.9999969
TACGAAT1700.041.02941512
CGAATAT1700.041.02941514
CGTTTTT39100.040.8567771
CGATGAA9250.039.40540319
CGGTCTA403.4584627E-739.37531
CGAAAGG1900.039.0789452
CCGATGA9300.038.70967518
ACACGAC1050.038.5714326
ACGCACG356.2486106E-638.5714261
CACGACC1000.038.24999627
GTATGCG659.094947E-1238.0769271
TAGGGTA3150.037.857145
CGGGTAT1200.037.4999966
ATTAGCG601.5643309E-1037.4999961
GCGCATT301.1399637E-437.49999645
CGAGGTA301.1399637E-437.4999964
CGGACGG1700.037.0588262
Produced by FastQC (version 0.11.5)