##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1220977
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67130339064536	31.0	31.0	34.0	30.0	34.0
2	31.819723876862547	33.0	31.0	34.0	30.0	34.0
3	31.765344474138335	33.0	31.0	34.0	30.0	34.0
4	35.45351304733832	37.0	35.0	37.0	33.0	37.0
5	33.40538191956114	37.0	35.0	37.0	30.0	37.0
6	34.31756781659278	37.0	35.0	37.0	30.0	37.0
7	35.42743966512064	37.0	35.0	37.0	32.0	37.0
8	35.35585191203438	37.0	35.0	37.0	33.0	37.0
9	37.24106350897683	39.0	37.0	39.0	34.0	39.0
10	36.72762468089079	39.0	35.0	39.0	32.0	39.0
11	36.82250853210175	39.0	37.0	39.0	33.0	39.0
12	36.87884702168837	39.0	37.0	39.0	33.0	39.0
13	37.01739836213131	39.0	37.0	39.0	33.0	39.0
14	38.07956824739532	40.0	37.0	41.0	33.0	41.0
15	38.16241501682669	40.0	37.0	41.0	33.0	41.0
16	38.215118712309895	40.0	37.0	41.0	34.0	41.0
17	38.066938197853034	40.0	37.0	41.0	33.0	41.0
18	38.03095062396753	40.0	37.0	41.0	33.0	41.0
19	37.93200199512358	40.0	37.0	41.0	33.0	41.0
20	38.05147189504798	40.0	37.0	41.0	34.0	41.0
21	37.93611837078012	40.0	37.0	41.0	33.0	41.0
22	38.06908156337097	40.0	37.0	41.0	34.0	41.0
23	38.11803088837873	40.0	37.0	41.0	34.0	41.0
24	38.007511197999634	40.0	37.0	41.0	34.0	41.0
25	37.695753482661836	40.0	36.0	41.0	33.0	41.0
26	37.80379728692678	40.0	36.0	41.0	33.0	41.0
27	37.4233601451952	40.0	36.0	41.0	33.0	41.0
28	37.45833869106461	40.0	36.0	41.0	33.0	41.0
29	37.716154358354004	40.0	36.0	41.0	33.0	41.0
30	37.458826824747725	40.0	36.0	41.0	33.0	41.0
31	37.436909130966434	40.0	36.0	41.0	33.0	41.0
32	37.50103318899537	40.0	36.0	41.0	33.0	41.0
33	37.49085855016106	40.0	36.0	41.0	33.0	41.0
34	37.364423736073654	40.0	36.0	41.0	33.0	41.0
35	37.3278702219616	40.0	36.0	41.0	33.0	41.0
36	37.12769200402629	40.0	36.0	41.0	32.0	41.0
37	37.14715019201836	40.0	36.0	41.0	32.0	41.0
38	36.97583328760493	40.0	35.0	41.0	31.0	41.0
39	36.90098257379132	40.0	35.0	41.0	31.0	41.0
40	36.80921262235079	39.0	35.0	41.0	31.0	41.0
41	36.66984554172601	39.0	35.0	41.0	31.0	41.0
42	36.8207550183173	39.0	35.0	41.0	31.0	41.0
43	36.766254401188554	39.0	35.0	41.0	31.0	41.0
44	36.8627787419419	40.0	35.0	41.0	31.0	41.0
45	36.85477285812919	39.0	35.0	41.0	31.0	41.0
46	36.72458449258258	39.0	35.0	41.0	31.0	41.0
47	36.67156629486059	39.0	35.0	41.0	31.0	41.0
48	36.6353567675722	39.0	35.0	41.0	31.0	41.0
49	36.59618485851904	39.0	35.0	41.0	31.0	41.0
50	36.52643415887441	39.0	35.0	41.0	31.0	41.0
51	35.65691655125363	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	32.0
9	46.0
10	49.0
11	63.0
12	52.0
13	50.0
14	77.0
15	71.0
16	104.0
17	206.0
18	371.0
19	578.0
20	1230.0
21	2223.0
22	3466.0
23	4935.0
24	6648.0
25	8675.0
26	10535.0
27	12328.0
28	13140.0
29	14590.0
30	17046.0
31	21792.0
32	29344.0
33	41849.0
34	70962.0
35	87516.0
36	100489.0
37	147572.0
38	255935.0
39	368888.0
40	110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.22041365234562	21.71695289919466	24.361146852070103	13.70148659638961
2	32.669329561490514	26.593375632792426	24.16400964145926	16.573285164257804
3	31.317297541231326	25.220458698239195	28.00495013419581	15.457293626333666
4	28.360566988567353	24.929789832240903	29.018974149390203	17.690669029801544
5	25.333237235427042	33.14001819854101	24.395463632812085	17.131280933219873
6	25.774605090841185	35.47568873123736	24.6926027271603	14.057103450761153
7	77.76542883281175	5.951627262430005	12.010873259692852	4.272070645065386
8	79.02417490255755	7.5234013417124155	9.33916036092408	4.113263394805963
9	72.06867942639377	8.553559976969263	11.376381373277301	8.001379223359653
10	38.34740539748087	31.164141503074998	17.782398849445975	12.706054249998159
11	28.919217970526883	25.160015299223492	28.52592636880138	17.39484036144825
12	27.129995077712355	22.318028922739742	30.221535704603774	20.33044029494413
13	24.966236055224627	23.058255806620437	31.571110676122483	20.404397462032453
14	18.86947911385718	28.42920055005131	30.440131140881444	22.261189195210065
15	18.976524537317246	26.99190893849761	34.10137946906453	19.930187055120612
16	21.671579399120542	27.167178415318226	30.826297301259565	20.33494488430167
17	20.984998079406903	28.025343638741763	29.51537989659101	21.47427838526033
18	21.171406177184338	27.448182889603984	29.89270068150342	21.487710251708265
19	23.009606241559013	27.777591224077113	28.022067573754462	21.190734960609415
20	25.608672399234383	26.908942592694213	29.7165302868113	17.765854721260105
21	24.46630853816247	27.643927772595223	29.08654298975329	18.803220699489014
22	22.43719578665282	24.93322970047757	30.50884660399008	22.120727908879527
23	22.802067524613488	26.871677353463664	29.98762466451047	20.338630457412386
24	22.015975730910576	25.973462235570366	29.86714737460247	22.143414658916587
25	21.009404763562294	29.545437792849498	27.79970466274139	21.645452780846814
26	20.564105630163386	28.336160304411955	29.76657217949232	21.33316188593233
27	22.133832168828732	29.02650909886099	28.412984028364168	20.4266747039461
28	19.677602444599692	29.11127728040741	30.28566467673019	20.9254555982627
29	20.818573978052	26.19467852383788	31.581020772709067	21.405726725401053
30	21.291146352470193	27.49060793118953	29.20775739428343	22.010488322056844
31	21.55077450271381	27.977267385053118	27.535735726389603	22.936222385843468
32	22.94760671167434	28.530430958158913	28.507662306497174	20.01430002366957
33	22.574708614494785	27.84687999855853	26.979951301293966	22.598460085652718
34	20.963703656989445	27.370376346155577	29.064675255963053	22.601244740891925
35	21.687140707810222	26.610411170726394	28.336324107661326	23.36612401380206
36	20.534375340403628	29.567469329889096	27.465300329162627	22.432855000544645
37	21.032091513599354	27.984065219901765	28.884737386535537	22.09910587996334
38	20.94740523367762	27.991518267747878	27.63180633214221	23.429270166432293
39	22.96284041386529	25.57836879810185	28.655822345547872	22.802968442484996
40	22.558246387933597	26.05552766350226	29.385320116595153	22.00090583196899
41	19.1136278570358	27.543516380734445	28.572774098119787	24.770081664109973
42	20.44657679874396	27.590527913302214	29.048540635900594	22.91435465205323
43	21.55167542058532	26.727284789148364	28.167442957565946	23.55359683270037
44	21.24921272063274	27.01189293492015	28.535754563763284	23.20313978068383
45	21.440371112641763	25.443968231997815	28.049750322897154	25.065910332463265
46	21.269606225178688	28.65352910005676	27.227048502961154	22.8498161718034
47	20.812840864324226	26.8138548064378	30.16330364945449	22.210000679783484
48	20.73462481275241	25.927187817624738	29.462471447046095	23.875715922576756
49	20.35009668486794	26.122031782744475	30.251593600862257	23.27627793152533
50	19.625840617800336	25.449783247350275	30.39827941066867	24.526096724180718
51	19.72248453492572	25.357070608209654	28.39283622869227	26.527608628172356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	483.0
1	953.0
2	1423.0
3	7919.0
4	14415.0
5	9439.5
6	4464.0
7	4272.0
8	4080.0
9	4022.0
10	3964.0
11	3875.5
12	3787.0
13	3748.0
14	3709.0
15	3607.5
16	3506.0
17	3515.5
18	3525.0
19	4061.0
20	4597.0
21	5421.0
22	6245.0
23	6510.0
24	6775.0
25	8207.5
26	12461.5
27	15283.0
28	18172.0
29	21061.0
30	24082.0
31	27103.0
32	29903.0
33	32703.0
34	36709.5
35	40716.0
36	44185.0
37	47654.0
38	50220.5
39	52787.0
40	56109.0
41	59431.0
42	64897.0
43	70363.0
44	75118.5
45	79874.0
46	96426.0
47	112978.0
48	107748.0
49	102518.0
50	99539.0
51	96560.0
52	85417.0
53	74274.0
54	67287.5
55	60301.0
56	56418.0
57	52535.0
58	50483.0
59	48431.0
60	48860.5
61	49290.0
62	44544.0
63	39798.0
64	33366.5
65	26935.0
66	21054.0
67	15173.0
68	12266.0
69	9359.0
70	7837.5
71	6316.0
72	5162.0
73	4008.0
74	3204.5
75	1842.5
76	1284.0
77	997.0
78	710.0
79	465.0
80	220.0
81	213.5
82	207.0
83	139.5
84	72.0
85	41.0
86	10.0
87	7.0
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1220977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.454587033387067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91469513202354	21.31666712713822
2	9.394427331157909	5.346291002465258
3	3.4307786449157183	2.9286416863212015
4	1.6599779333050653	1.8893594630692387
5	1.008682638948043	1.4350823969506816
6	0.723630475743109	1.2354363811226148
7	0.5534597263548304	1.102392756712668
8	0.4697521951844359	1.0693283777600129
9	0.43069300298963237	1.1029672384415397
>10	6.873902595946752	47.90213334611346
>50	0.4908570014530032	8.37596780327364
>100	0.04012886646788033	1.9578595907779286
>500	0.004652622198722807	0.8804658906357825
>1k	0.0034894666490421053	1.762586636812653
>5k	8.723666622605263E-4	1.6948203024051063
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8451	0.6921506301920511	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	6677	0.546857148005245	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	5355	0.43858320017494185	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	4822	0.39492963421915406	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2983	0.2443125464279835	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1590	0.13022358324522085	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	1541	0.12621040363577693	No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	1539	0.12604660038641186	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC	1460	0.11957637203649207	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1407	0.11523558592831805	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1386	0.11351565180998495	TruSeq Adapter, Index 13 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1302	0.10663591533665254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.457048740475865E-4	0.0	0.0	0.1332539433584744	0.0
2	2.457048740475865E-4	0.0	0.0	0.6243360849549172	0.0
3	2.457048740475865E-4	0.0	0.0	0.8443238488521897	0.0
4	2.457048740475865E-4	0.0	0.0	1.193306671624445	0.0
5	2.457048740475865E-4	0.0	0.0	2.3950492105911905	0.0
6	2.457048740475865E-4	0.0	0.0	3.125529801134665	0.0
7	2.457048740475865E-4	0.0	0.0	3.730619004289188	0.0
8	2.457048740475865E-4	0.0	0.0	4.589685145584233	0.0
9	2.457048740475865E-4	0.0	0.0	4.963484160635295	0.0
10	2.457048740475865E-4	0.0	0.0	6.131974639980934	0.0
11	2.457048740475865E-4	0.0	0.0	7.0859647642830295	0.0
12	2.457048740475865E-4	0.0	0.0	8.245282261664224	0.0
13	2.457048740475865E-4	0.0	0.0	8.63382356915814	0.0
14	2.457048740475865E-4	0.0	0.0	8.813024323963514	0.0
15	2.457048740475865E-4	0.0	0.0	9.099106698979588	0.0
16	2.457048740475865E-4	0.0	0.0	9.542194488512068	0.0
17	2.457048740475865E-4	0.0	0.0	10.04261341532232	0.0
18	3.276064987301153E-4	0.0	0.0	10.603803347647007	0.0
19	3.276064987301153E-4	0.0	0.0	11.062616249118534	0.0
20	3.276064987301153E-4	0.0	0.0	11.460985751574354	0.0
21	3.276064987301153E-4	0.0	0.0	11.900797476119534	0.0
22	3.276064987301153E-4	0.0	0.0	12.392534830713437	0.0
23	3.276064987301153E-4	0.0	0.0	12.840782422600917	0.0
24	3.276064987301153E-4	0.0	0.0	13.20008485008317	0.0
25	4.0950812341264414E-4	0.0	0.0	13.525316201697493	0.0
26	4.91409748095173E-4	0.0	0.0	13.845633455830864	0.0
27	4.91409748095173E-4	0.0	0.0	14.158661465367489	0.0
28	4.91409748095173E-4	0.0	0.0	14.468249606667447	0.0
29	4.91409748095173E-4	0.0	0.0	14.799377875258912	0.0
30	4.91409748095173E-4	0.0	0.0	15.18505262588894	0.0
31	6.552129974602306E-4	0.0	0.0	15.529121351180244	0.0
32	7.371146221427595E-4	0.0	0.0	15.868767388738691	0.0
33	7.371146221427595E-4	0.0	0.0	16.19457205172579	0.0
34	7.371146221427595E-4	0.0	0.0	16.525454615443206	0.0
35	7.371146221427595E-4	0.0	0.0	16.868294816364273	0.0
36	7.371146221427595E-4	0.0	0.0	17.228825768216765	0.0
37	9.009178715078171E-4	0.0	0.0	17.593124194804652	0.0
38	9.009178715078171E-4	0.0	0.0	17.941451804579447	0.0
39	9.82819496190346E-4	0.0	0.0	18.294775413459877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	65	0.0	45.000004	1
CGATCGG	20	7.03346E-4	45.0	10
GCGATCG	25	3.89095E-5	44.999996	9
TACGAAT	170	0.0	41.029415	12
CGAATAT	170	0.0	41.029415	14
CGTTTTT	3910	0.0	40.856777	1
CGATGAA	925	0.0	39.405403	19
CGGTCTA	40	3.4584627E-7	39.375	31
CGAAAGG	190	0.0	39.078945	2
CCGATGA	930	0.0	38.709675	18
ACACGAC	105	0.0	38.57143	26
ACGCACG	35	6.2486106E-6	38.571426	1
CACGACC	100	0.0	38.249996	27
GTATGCG	65	9.094947E-12	38.076927	1
TAGGGTA	315	0.0	37.85714	5
CGGGTAT	120	0.0	37.499996	6
ATTAGCG	60	1.5643309E-10	37.499996	1
GCGCATT	30	1.1399637E-4	37.499996	45
CGAGGTA	30	1.1399637E-4	37.499996	4
CGGACGG	170	0.0	37.058826	2
>>END_MODULE