FastQCFastQC Report
Sat 18 Jun 2016
SRR3551360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640532
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG57960.9048728244646637No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC49990.7804450050895193No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC48600.758744293805774No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41070.6411857643333979No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC18870.2945988646937234No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAAGCGT14570.22746716791666927No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT11270.17594749364590684No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCAACCGTCGAATGC10460.1633017554158106No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTA8510.13285831152854188No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT8480.13238995085335317No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7500.11709016879718732No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTC6590.10288322831646195No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT6500.1014781462908957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACG302.1639898E-645.0000041
TCGTTGC207.030946E-445.038
CGACGTA207.030946E-445.045
CGTTGCA207.030946E-445.039
CCCGTCG502.1827873E-1145.040
CGCTCAC207.030946E-445.034
GACGGGT750.045.04
TTAGTCG207.030946E-445.01
CGTGTAA253.8888647E-545.026
ATGCGCC207.030946E-445.022
TGTCGTG207.030946E-445.01
AGGTGCG253.8888647E-545.01
TCGGTCG207.030946E-445.01
ATACCGA253.8888647E-545.020
TACCGTG207.030946E-445.032
CCGATGA15750.043.71428318
AACCGTC2200.042.95454439
GCAACCG1950.042.69230737
GTCGAAT2600.042.4038543
ACCCGTC750.042.039