##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	640532
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.621207059132097	31.0	31.0	34.0	30.0	34.0
2	31.77786746017373	31.0	31.0	34.0	30.0	34.0
3	31.730689489361968	33.0	31.0	34.0	30.0	34.0
4	35.46231257766981	37.0	35.0	37.0	33.0	37.0
5	33.39980828436362	37.0	35.0	37.0	30.0	37.0
6	34.303457126263794	37.0	35.0	37.0	30.0	37.0
7	35.39122635559191	37.0	35.0	37.0	32.0	37.0
8	35.26947755927885	37.0	35.0	37.0	33.0	37.0
9	37.076439584595306	39.0	37.0	39.0	33.0	39.0
10	36.58177421268571	38.0	35.0	39.0	32.0	39.0
11	36.720190404226486	39.0	35.0	39.0	32.0	39.0
12	36.74485583858418	39.0	35.0	39.0	33.0	39.0
13	36.8119094752487	39.0	37.0	39.0	33.0	39.0
14	37.86960526562295	40.0	37.0	41.0	33.0	41.0
15	37.95958515733796	40.0	37.0	41.0	33.0	41.0
16	38.07179813030419	40.0	37.0	41.0	33.0	41.0
17	37.85828654930589	40.0	37.0	41.0	33.0	41.0
18	37.87998101578063	39.0	37.0	41.0	33.0	41.0
19	37.74726321245465	39.0	37.0	41.0	33.0	41.0
20	37.68853546739273	39.0	36.0	41.0	33.0	41.0
21	37.721401897172974	39.0	36.0	41.0	33.0	41.0
22	37.82449744899552	40.0	36.0	41.0	33.0	41.0
23	37.75774044075862	40.0	36.0	41.0	33.0	41.0
24	37.710412594530794	40.0	36.0	41.0	33.0	41.0
25	37.51498129679704	39.0	35.0	41.0	33.0	41.0
26	37.496958778015774	39.0	36.0	41.0	33.0	41.0
27	37.0678639006326	40.0	35.0	41.0	32.0	41.0
28	37.12709591402147	40.0	35.0	41.0	32.0	41.0
29	37.3507350140196	40.0	35.0	41.0	32.0	41.0
30	37.12711620965073	39.0	35.0	41.0	32.0	41.0
31	37.11698244584189	39.0	35.0	41.0	32.0	41.0
32	36.97372340491966	39.0	35.0	41.0	31.0	41.0
33	36.91521266697058	40.0	35.0	41.0	31.0	41.0
34	36.72007955886669	40.0	35.0	41.0	31.0	41.0
35	36.647480531807936	40.0	35.0	41.0	30.0	41.0
36	36.48111257517189	40.0	35.0	41.0	30.0	41.0
37	36.31965147720957	40.0	35.0	41.0	30.0	41.0
38	36.318855264061746	39.0	35.0	41.0	29.0	41.0
39	36.262338181386724	39.0	35.0	41.0	30.0	41.0
40	36.140341153915806	39.0	35.0	41.0	29.0	41.0
41	36.03637601243966	39.0	35.0	41.0	28.0	41.0
42	36.11405675282421	39.0	35.0	41.0	29.0	41.0
43	35.99952383331356	39.0	35.0	41.0	29.0	41.0
44	36.12673215389707	39.0	35.0	41.0	29.0	41.0
45	36.134177527430325	39.0	35.0	41.0	29.0	41.0
46	36.03107416959652	39.0	35.0	41.0	28.0	41.0
47	35.94076486420663	39.0	35.0	41.0	28.0	41.0
48	35.90476978511612	39.0	35.0	41.0	28.0	41.0
49	35.85986024117452	39.0	35.0	41.0	28.0	41.0
50	35.75460398543711	39.0	35.0	41.0	28.0	41.0
51	34.88855826094559	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	16.0
10	23.0
11	29.0
12	24.0
13	24.0
14	31.0
15	40.0
16	74.0
17	128.0
18	227.0
19	506.0
20	1137.0
21	2350.0
22	3868.0
23	5019.0
24	5767.0
25	6787.0
26	7749.0
27	7714.0
28	7766.0
29	7823.0
30	9366.0
31	12116.0
32	15908.0
33	22870.0
34	37705.0
35	47714.0
36	51761.0
37	75860.0
38	129080.0
39	180989.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.098549330868714	20.467205385523286	23.377754741371234	13.056490542236766
2	31.39171813430086	29.156857112525213	24.200664447677866	15.250760305496055
3	29.89655473887331	27.88494563893763	27.487307425702383	14.73119219648667
4	26.760411657809446	24.190516633048777	32.67346518206741	16.375606527074368
5	28.059644170783038	31.465406880530562	23.98194001236472	16.493008936321683
6	24.061405206921744	37.69757014481712	24.56489293274965	13.676131715511481
7	75.65601718571438	5.459836510900314	15.112125545640186	3.7720207577451244
8	77.02940680559284	10.297221684474781	9.190485409003765	3.4828861009286034
9	71.03891764970368	7.930907433196157	10.400729393691494	10.629445523408666
10	40.93331792947112	27.439534636833134	18.38674726633486	13.24040016736088
11	31.143955337126012	24.110739198041628	27.11433620802708	17.630969256805283
12	25.652270300312864	21.88181074481837	29.498448164962877	22.967470789905892
13	25.651021338512365	23.707324536479053	32.213066638356864	18.428587486651722
14	19.853809021251084	30.371784703964828	29.275196243122902	20.499210031661182
15	19.243222821030017	26.391655686210836	33.6245495931507	20.74057189960845
16	21.714605983775986	25.837116646787354	29.720919485677534	22.727357883759126
17	21.779864237852287	29.25521285431485	29.07442563369199	19.89049727414087
18	21.60891259140839	26.461753667264087	29.63989308886988	22.289440652457646
19	22.120830809389695	27.74162727232988	27.966752636870602	22.170789281409828
20	25.866623369324248	26.888898603036225	30.32042114991913	16.924056877720396
21	22.9198541212617	30.387240606246056	29.298302036432215	17.394603236060025
22	21.91131746735526	24.422979648167463	32.263181230602065	21.40252165387522
23	23.55947868334447	26.706394059937676	30.05392392573673	19.680203330981122
24	21.26295017266897	28.12849319003578	29.972585288478953	20.635971348816295
25	20.256443081688346	32.22758581928772	27.842324817495456	19.673646281528477
26	19.54734501945258	28.619959658533844	31.18126807091605	20.651427251097527
27	23.029138278805743	29.504068493065137	28.224819368899603	19.241973859229518
28	18.70320296253739	30.166798848457223	31.16893457313608	19.96106361586931
29	20.772888786196475	26.786015374719764	32.14265641685349	20.29843942223027
30	21.05640311491073	31.083068449351476	28.76483922739223	19.09568920834556
31	22.009673209144896	26.93776423348092	26.53153940786721	24.521023149506973
32	24.21752543198466	29.665809046230322	26.56057776972891	19.556087752056104
33	23.921677605490434	28.15878051369799	26.15778758906659	21.761754291744985
34	21.509932368718506	28.06307881573442	27.167104844098343	23.259883971448733
35	24.455452654980547	26.847370623169493	26.917468604222737	21.779708117627223
36	22.548444105837024	31.328333322925317	25.07587442938058	21.047348141857082
37	22.790586574909607	28.564380858411447	27.95800990426708	20.68702266241187
38	20.32560434139122	29.367151055684964	25.840239051288616	24.467005551635204
39	23.053180793465433	29.81880686679198	26.96602199421731	20.161990345525282
40	21.444205753967015	28.196717728388276	27.414399280598	22.944677237046704
41	21.502750838365607	27.003646968457467	26.66642728232157	24.82717491085535
42	21.08544147677243	26.744955755528217	28.18953619803538	23.980066569663965
43	23.349965341310035	27.981740178476645	26.318123060206204	22.35017142000712
44	21.830759431222795	27.293562226399303	28.24183647343146	22.63384186894644
45	20.934473219136592	27.111369923750882	27.10481287429824	24.849343982814283
46	23.30890572211849	29.936989877164606	25.92345113124715	20.830653269469753
47	20.554944952008643	28.768742233018802	29.68407511256268	20.99223770240987
48	21.714918224226114	27.755365852135412	27.84419826019621	22.685517663442266
49	21.495725428237776	26.057402284351134	30.28623082063035	22.16064146678074
50	21.54989914633461	26.7471414386791	28.605752718053118	23.097206696933174
51	20.646275283670448	26.11329332492366	27.57910611803938	25.661325273366515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	166.0
1	434.0
2	702.0
3	4009.5
4	7317.0
5	4966.0
6	2615.0
7	2496.5
8	2378.0
9	2378.0
10	2378.0
11	2346.0
12	2314.0
13	2323.0
14	2332.0
15	2251.0
16	2170.0
17	2128.0
18	2086.0
19	2223.0
20	2360.0
21	2668.0
22	2976.0
23	3091.0
24	3206.0
25	4112.5
26	5665.0
27	6311.0
28	8321.5
29	10332.0
30	12375.0
31	14418.0
32	15770.5
33	17123.0
34	19669.5
35	22216.0
36	23426.0
37	24636.0
38	25863.0
39	27090.0
40	29838.0
41	32586.0
42	35214.0
43	37842.0
44	39412.5
45	40983.0
46	42113.5
47	43244.0
48	54901.0
49	66558.0
50	59380.0
51	52202.0
52	51012.5
53	49823.0
54	44483.5
55	39144.0
56	34481.5
57	29819.0
58	26983.5
59	24148.0
60	22137.0
61	20126.0
62	17458.5
63	14791.0
64	12587.5
65	10384.0
66	8886.5
67	7389.0
68	6058.0
69	4727.0
70	3723.5
71	2720.0
72	2171.0
73	1622.0
74	1419.5
75	867.5
76	518.0
77	399.0
78	280.0
79	214.0
80	148.0
81	102.0
82	56.0
83	51.5
84	47.0
85	29.0
86	11.0
87	6.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	640532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.61826760932332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16540526452074	24.84383571490701
2	8.356795035098006	5.451683536221825
3	3.1705856683887057	3.102570354293641
4	1.7684514835226908	2.307352949745918
5	1.163772566914706	1.8980122512006505
6	0.8907430696437344	1.7432697490073674
7	0.7631072653122467	1.7423865896201631
8	0.6084290946090204	1.5876722423404261
9	0.5087836491947294	1.4936077102213585
>10	6.396709839950916	46.034799063006595
>50	0.17340542088866331	3.3817365589112796
>100	0.026566290108122666	1.6514798085311033
>500	0.0033811641955792484	0.7787888469346731
>1k	0.0033811641955792484	3.0696223153607596
>5k	4.8302345651132127E-4	0.9131823096972214
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG	5796	0.9048728244646637	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC	4999	0.7804450050895193	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC	4860	0.758744293805774	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4107	0.6411857643333979	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1887	0.2945988646937234	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAAGCGT	1457	0.22746716791666927	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT	1127	0.17594749364590684	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCAACCGTCGAATGC	1046	0.1633017554158106	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTA	851	0.13285831152854188	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	848	0.13238995085335317	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	750	0.11709016879718732	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTC	659	0.10288322831646195	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	650	0.1014781462908957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10694235416809778	0.0
2	0.0	0.0	0.0	0.5788937945332941	0.0
3	0.0	0.0	0.0	0.7292375712688828	0.0
4	0.0	0.0	0.0	1.0196211898859073	0.0
5	0.0	0.0	0.0	2.225649928496937	0.0
6	0.0	0.0	0.0	2.6502969406680696	0.0
7	0.0	0.0	0.0	3.1875066351095653	0.0
8	0.0	0.0	0.0	3.8035570432078334	0.0
9	0.0	0.0	0.0	4.02446716167186	0.0
10	0.0	0.0	0.0	5.499491048066295	0.0
11	0.0	0.0	0.0	6.281965616081632	0.0
12	0.0	0.0	0.0	7.724828736113106	0.0
13	0.0	0.0	0.0	8.033946781737681	0.0
14	0.0	0.0	0.0	8.184446678698333	0.0
15	0.0	0.0	0.0	8.512299151330456	0.0
16	0.0	0.0	0.0	8.936009442151212	0.0
17	0.0	0.0	0.0	9.420606620746504	0.0
18	0.0	0.0	0.0	9.925343308374913	0.0
19	0.0	0.0	0.0	10.516258360238051	0.0
20	0.0	0.0	0.0	10.853009685698764	0.0
21	0.0	0.0	0.0	11.228478826975078	0.0
22	0.0	0.0	0.0	11.637201576189792	0.0
23	0.0	0.0	0.0	12.037806073701235	0.0
24	0.0	0.0	0.0	12.364097344082731	0.0
25	0.0	0.0	0.0	12.649641235722806	0.0
26	0.0	0.0	0.0	12.93893201276439	0.0
27	0.0	0.0	0.0	13.238682844885188	0.0
28	0.0	0.0	0.0	13.535779633179919	0.0
29	0.0	0.0	0.0	13.850361886681696	0.0
30	0.0	0.0	0.0	14.189611135743414	0.0
31	0.0	0.0	0.0	14.515902406124908	0.0
32	0.0	0.0	0.0	14.826581654000112	0.0
33	0.0	0.0	0.0	15.139758825476322	0.0
34	0.0	0.0	0.0	15.437323974446242	0.0
35	0.0	0.0	0.0	15.754560271774087	0.0
36	0.0	0.0	0.0	16.06836192415055	0.0
37	1.5612022506291645E-4	0.0	0.0	16.397307238358113	0.0
38	1.5612022506291645E-4	0.0	0.0	16.707674245783192	0.0
39	1.5612022506291645E-4	0.0	0.0	17.039273603816827	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGACG	30	2.1639898E-6	45.000004	1
TCGTTGC	20	7.030946E-4	45.0	38
CGACGTA	20	7.030946E-4	45.0	45
CGTTGCA	20	7.030946E-4	45.0	39
CCCGTCG	50	2.1827873E-11	45.0	40
CGCTCAC	20	7.030946E-4	45.0	34
GACGGGT	75	0.0	45.0	4
TTAGTCG	20	7.030946E-4	45.0	1
CGTGTAA	25	3.8888647E-5	45.0	26
ATGCGCC	20	7.030946E-4	45.0	22
TGTCGTG	20	7.030946E-4	45.0	1
AGGTGCG	25	3.8888647E-5	45.0	1
TCGGTCG	20	7.030946E-4	45.0	1
ATACCGA	25	3.8888647E-5	45.0	20
TACCGTG	20	7.030946E-4	45.0	32
CCGATGA	1575	0.0	43.714283	18
AACCGTC	220	0.0	42.954544	39
GCAACCG	195	0.0	42.692307	37
GTCGAAT	260	0.0	42.40385	43
ACCCGTC	75	0.0	42.0	39
>>END_MODULE