FastQCFastQC Report
Sat 18 Jun 2016
SRR3551357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895592
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51560.575708581586258No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC16010.1787644373777345No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC15560.17373982795737344No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC14950.16692869074310623No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT10600.11835746634628268No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC9230.10306032211096124No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC9000.10049218840722114No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATA207.03245E-445.00000424
TTAAGCG800.045.0000041
CTACGCG207.03245E-445.0000041
TCGACGT207.03245E-445.00000425
TACACCG207.03245E-445.00000418
ACGGTTG207.03245E-445.0000041
ACGAACC207.03245E-445.00000410
CCCGTCG253.8901133E-545.032
TACGCCC351.2115197E-745.038
TACGGGT302.164963E-644.9999964
GCTACGA1000.042.7510
CGTTTTT24250.042.4020581
CGTAAGG1700.041.029412
TATAGCG556.002665E-1140.9090921
TACGTAG556.002665E-1140.9090921
GTTAGCG451.9274012E-840.0000041
GCCGATT403.4570803E-739.3750049
TATTACG403.4570803E-739.3750041
CAATACG403.4570803E-739.37500435
TAGTACG403.4570803E-739.3750041