##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	895592
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.696593984760916	33.0	31.0	34.0	30.0	34.0
2	31.837393589938277	33.0	31.0	34.0	30.0	34.0
3	31.774484363415485	33.0	31.0	34.0	30.0	34.0
4	35.46368882258886	37.0	35.0	37.0	33.0	37.0
5	33.40204803079974	37.0	35.0	37.0	30.0	37.0
6	34.36298671716585	37.0	35.0	37.0	30.0	37.0
7	35.52165941634137	37.0	35.0	37.0	32.0	37.0
8	35.58423255232293	37.0	35.0	37.0	33.0	37.0
9	37.551881883714906	39.0	37.0	39.0	35.0	39.0
10	36.89043336697961	39.0	37.0	39.0	33.0	39.0
11	36.92764674092667	39.0	37.0	39.0	33.0	39.0
12	37.02793906153695	39.0	37.0	39.0	33.0	39.0
13	37.192118732637184	39.0	37.0	39.0	34.0	39.0
14	38.240875309292626	40.0	38.0	41.0	34.0	41.0
15	38.36694164306961	40.0	38.0	41.0	34.0	41.0
16	38.3833330355787	40.0	38.0	41.0	34.0	41.0
17	38.26952674878739	40.0	38.0	41.0	34.0	41.0
18	38.23580045377806	40.0	37.0	41.0	34.0	41.0
19	38.137243298287615	40.0	37.0	41.0	34.0	41.0
20	38.3215403889271	40.0	37.0	41.0	34.0	41.0
21	38.20809922375367	40.0	37.0	41.0	34.0	41.0
22	38.3625624168148	40.0	37.0	41.0	34.0	41.0
23	38.424789413036294	40.0	38.0	41.0	34.0	41.0
24	38.301058964349835	40.0	37.0	41.0	34.0	41.0
25	37.9792952594485	40.0	37.0	41.0	34.0	41.0
26	38.13058289935596	40.0	37.0	41.0	34.0	41.0
27	37.777988191051286	40.0	37.0	41.0	34.0	41.0
28	37.842432714896965	40.0	37.0	41.0	33.0	41.0
29	38.07935309828583	40.0	37.0	41.0	34.0	41.0
30	37.831674467838035	40.0	37.0	41.0	33.0	41.0
31	37.81016020687992	40.0	37.0	41.0	33.0	41.0
32	37.93826429892183	40.0	37.0	41.0	34.0	41.0
33	37.95149577039545	40.0	37.0	41.0	34.0	41.0
34	37.83708876363344	40.0	37.0	41.0	34.0	41.0
35	37.81991018231516	40.0	37.0	41.0	33.0	41.0
36	37.63134775656772	40.0	37.0	41.0	33.0	41.0
37	37.635819658951846	40.0	37.0	41.0	33.0	41.0
38	37.49202538655995	40.0	37.0	41.0	33.0	41.0
39	37.44882602792343	40.0	37.0	41.0	33.0	41.0
40	37.35349913800034	40.0	36.0	41.0	33.0	41.0
41	37.206636504122415	40.0	36.0	41.0	32.0	41.0
42	37.38453112578049	40.0	36.0	41.0	33.0	41.0
43	37.311895371999746	40.0	36.0	41.0	33.0	41.0
44	37.368450142475595	40.0	36.0	41.0	33.0	41.0
45	37.35842995471152	40.0	36.0	41.0	33.0	41.0
46	37.25449758372116	40.0	36.0	41.0	32.0	41.0
47	37.227863804053634	40.0	36.0	41.0	32.0	41.0
48	37.18823526784518	40.0	36.0	41.0	32.0	41.0
49	37.14646178170417	40.0	36.0	41.0	32.0	41.0
50	37.077027262414134	39.0	35.0	41.0	32.0	41.0
51	36.20343973595119	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	19.0
9	24.0
10	40.0
11	44.0
12	39.0
13	37.0
14	49.0
15	42.0
16	74.0
17	105.0
18	214.0
19	320.0
20	544.0
21	909.0
22	1381.0
23	2117.0
24	3134.0
25	4675.0
26	6293.0
27	7627.0
28	8225.0
29	9319.0
30	11094.0
31	14419.0
32	19276.0
33	27316.0
34	46077.0
35	59658.0
36	71444.0
37	107651.0
38	195192.0
39	298140.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.45543059786153	23.45699827600068	27.746563167156474	13.341007958981322
2	32.3981232525525	24.99397046869557	26.252244325541096	16.355661953210838
3	31.2631198134865	23.374929655468115	29.06178259743276	16.300167933612627
4	28.09772753664615	25.959588741301843	27.96999079938186	17.972692922670145
5	24.052581979294143	33.540496118768374	25.140465747795872	17.26645615414162
6	26.192395644445238	33.4893567606678	24.97275545114293	15.345492143744027
7	82.0345648464926	4.756406935300896	9.0576959151042	4.151332303102306
8	84.32880150782945	4.221341861026003	7.827671528999812	3.622185102144727
9	77.42699800802151	8.070304335009693	9.728648759703079	4.774048897265719
10	33.30690760971514	40.852977695200494	15.490535869011781	10.349578826072586
11	22.761145700274234	25.127848395251405	34.50220636182547	17.608799542648885
12	23.903853540451454	21.777327175767535	35.32412080500942	18.994698478771582
13	22.769743365282405	23.196835166013095	35.241493894541264	18.791927574163235
14	18.064475788082074	27.770346318412848	33.105141626990864	21.060036266514217
15	17.356899123708118	28.255500272445488	36.201975899740056	18.185624704106335
16	20.69938096811941	28.00862446292508	33.12412348480112	18.167871084154392
17	20.55221574109639	27.491424666589246	30.65145736004788	21.30490223226648
18	20.02128201234491	27.72534814960384	32.14856765134124	20.104802186710018
19	21.15583881946243	28.337010603042458	31.05208621783133	19.455064359663776
20	22.833500075927432	28.22535261592332	31.02193856130917	17.91920874684008
21	22.816304745911083	27.00493081671118	32.31929271364639	17.859471723731343
22	21.100233141877105	25.5887725660792	31.24782266925118	22.06317162279252
23	19.688876184691242	26.935032916774603	32.657616414617365	20.718474483916783
24	20.077222663891593	26.338667607571303	33.00085306702159	20.583256661515513
25	19.173797890110674	29.27571930075302	29.647875371821097	21.902607437315204
26	18.759658415885806	30.71097106718238	30.129232954291684	20.40013756264013
27	19.57040706035784	29.977154775835423	30.898444827555405	19.55399333625133
28	17.846966029174	28.943983421022075	32.69770163199314	20.51134891781079
29	19.20830020813049	26.759618219010445	32.83861401173749	21.193467561121583
30	20.499401513189042	27.265428900660122	31.14967529857346	21.085494287577376
31	20.26201663257376	28.24131970808136	30.24513394492135	21.25152971442353
32	20.487565766554415	28.83321869779989	29.79872531241905	20.880490223226648
33	20.905054980392855	27.535194597539952	30.18316376207023	21.376586659996963
34	20.529660827698326	26.818796952183583	30.857131372321323	21.79441084779676
35	19.950044216562898	26.282838614011737	30.424233356260437	23.342883813164924
36	21.00409561496753	27.74019866189068	30.135709117544597	21.11999660559719
37	20.57946028995346	27.315005046941014	31.53299716835345	20.572537494752076
38	20.07286800239395	26.463501237170494	31.243244691779292	22.220386068656264
39	22.16567365496789	25.318783553225128	31.49391687286175	21.021625918945233
40	22.605941098178636	25.469968467784437	31.477503148755236	20.44658728528169
41	20.517378449115224	26.609773200296566	29.50953112578049	23.363317224807727
42	21.42314804062564	26.592466212293097	30.144418440539887	21.83996730654137
43	21.99774004234071	25.98259028664838	30.115722337850272	21.903947333160637
44	20.571197598906647	26.41705151452894	30.929709063948764	22.082041822615654
45	20.837055266237304	25.378967208282344	30.296049987047674	23.48792753843268
46	21.033015033631386	26.917949244745376	29.75707688322361	22.29195883839963
47	20.08314053720891	25.699313973327143	31.623663453894185	22.593882035569766
48	20.173583506775405	24.90062439146397	31.62500334973961	23.30078875202101
49	20.709541844947253	24.511719622328023	32.25062305156812	22.5281154811566
50	19.58224280699247	25.129523265058197	31.375894380476826	23.91233954747251
51	19.385166459727195	24.473867564694636	30.20426712163575	25.93669885394242
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	196.0
1	614.0
2	1032.0
3	4705.5
4	8379.0
5	5491.5
6	2604.0
7	2599.0
8	2594.0
9	2542.5
10	2491.0
11	2539.5
12	2588.0
13	2700.5
14	2813.0
15	2820.5
16	2828.0
17	3016.5
18	3205.0
19	3576.5
20	3948.0
21	4251.0
22	4554.0
23	5073.0
24	5592.0
25	7437.5
26	11228.0
27	13173.0
28	16548.0
29	19923.0
30	20877.5
31	21832.0
32	25518.5
33	29205.0
34	32611.5
35	36018.0
36	38540.0
37	41062.0
38	43698.5
39	46335.0
40	49052.5
41	51770.0
42	55762.5
43	59755.0
44	63639.0
45	67523.0
46	74235.5
47	80948.0
48	83393.0
49	85838.0
50	80740.0
51	75642.0
52	64304.5
53	52967.0
54	45376.5
55	37786.0
56	34422.5
57	31059.0
58	28778.5
59	26498.0
60	24888.0
61	23278.0
62	20697.5
63	18117.0
64	14432.5
65	10748.0
66	8307.5
67	5867.0
68	4475.0
69	3083.0
70	2674.5
71	2266.0
72	1784.0
73	1302.0
74	1001.5
75	581.0
76	461.0
77	307.0
78	153.0
79	129.5
80	106.0
81	80.0
82	54.0
83	30.5
84	7.0
85	4.0
86	1.0
87	2.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	895592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.012268613699508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52415323897691	22.801507898419565
2	10.483644226286806	6.502431815921327
3	3.833136400582885	3.566227670634771
4	1.902755942279893	2.3603511355318783
5	1.1226040535462696	1.7407249227702408
6	0.7778219591139514	1.447321411780551
7	0.5991861392375671	1.300748504974869
8	0.48906252363028374	1.2133550681372898
9	0.4120893188649149	1.1501842184527686
>10	6.43642976863433	47.099953663454386
>50	0.3799500934631308	7.064306179564806
>100	0.034407808654331004	1.8708813992196875
>500	0.0029283241407941107	0.6482982629053817
>1k	0.0014641620703970553	0.6484117803739347
>5k	3.6604051759926384E-4	0.5852960678585446
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5156	0.575708581586258	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1601	0.1787644373777345	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCC	1556	0.17373982795737344	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1495	0.16692869074310623	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	1060	0.11835746634628268	No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	923	0.10306032211096124	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	900	0.10049218840722114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.116579871191346E-4	0.0	0.0	0.12170720595985672	0.0
2	1.116579871191346E-4	0.0	0.0	0.6622435216035873	0.0
3	1.116579871191346E-4	0.0	0.0	0.9674048004001822	0.0
4	1.116579871191346E-4	0.0	0.0	1.3681453161707564	0.0
5	1.116579871191346E-4	0.0	0.0	2.433585829261539	0.0
6	1.116579871191346E-4	0.0	0.0	3.1477503148755237	0.0
7	1.116579871191346E-4	0.0	0.0	3.8528705035328588	0.0
8	1.116579871191346E-4	0.0	0.0	4.939414376189157	0.0
9	1.116579871191346E-4	0.0	0.0	5.400785178965422	0.0
10	1.116579871191346E-4	0.0	0.0	6.430495136178081	0.0
11	1.116579871191346E-4	0.0	0.0	7.882942232623784	0.0
12	1.116579871191346E-4	0.0	0.0	9.094319734879276	0.0
13	1.116579871191346E-4	0.0	0.0	9.607611501665938	0.0
14	1.116579871191346E-4	0.0	0.0	9.828694316161824	0.0
15	1.116579871191346E-4	0.0	0.0	10.120233320529884	0.0
16	1.116579871191346E-4	0.0	0.0	10.72966261422612	0.0
17	1.116579871191346E-4	0.0	0.0	11.545659184092756	0.0
18	1.116579871191346E-4	0.0	0.0	12.418043037454556	0.0
19	1.116579871191346E-4	0.0	0.0	12.992076749234027	0.0
20	1.116579871191346E-4	0.0	0.0	13.51284960115767	0.0
21	2.233159742382692E-4	0.0	0.0	14.130429927913603	0.0
22	2.233159742382692E-4	0.0	0.0	14.81991799837426	0.0
23	2.233159742382692E-4	0.0	0.0	15.448440807867868	0.0
24	2.233159742382692E-4	0.0	0.0	15.906461871030558	0.0
25	2.233159742382692E-4	0.0	0.0	16.31412518200252	0.0
26	2.233159742382692E-4	0.0	0.0	16.70995274633985	0.0
27	2.233159742382692E-4	0.0	0.0	17.126102064332866	0.0
28	2.233159742382692E-4	0.0	0.0	17.53979490660926	0.0
29	2.233159742382692E-4	0.0	0.0	17.98240716754951	0.0
30	2.233159742382692E-4	0.0	0.0	18.50128183369213	0.0
31	2.233159742382692E-4	0.0	0.0	18.97314848725759	0.0
32	3.349739613574038E-4	0.0	0.0	19.399570340065566	0.0
33	4.466319484765384E-4	0.0	0.0	19.821972505337254	0.0
34	4.466319484765384E-4	0.0	0.0	20.26871611180091	0.0
35	4.466319484765384E-4	0.0	0.0	20.734218260100583	0.0
36	4.466319484765384E-4	0.0	0.0	21.180068602667287	0.0
37	4.466319484765384E-4	0.0	0.0	21.647022304799506	0.0
38	5.58289935595673E-4	0.0	0.0	22.08025529482175	0.0
39	5.58289935595673E-4	0.0	0.0	22.552121948387214	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACATA	20	7.03245E-4	45.000004	24
TTAAGCG	80	0.0	45.000004	1
CTACGCG	20	7.03245E-4	45.000004	1
TCGACGT	20	7.03245E-4	45.000004	25
TACACCG	20	7.03245E-4	45.000004	18
ACGGTTG	20	7.03245E-4	45.000004	1
ACGAACC	20	7.03245E-4	45.000004	10
CCCGTCG	25	3.8901133E-5	45.0	32
TACGCCC	35	1.2115197E-7	45.0	38
TACGGGT	30	2.164963E-6	44.999996	4
GCTACGA	100	0.0	42.75	10
CGTTTTT	2425	0.0	42.402058	1
CGTAAGG	170	0.0	41.02941	2
TATAGCG	55	6.002665E-11	40.909092	1
TACGTAG	55	6.002665E-11	40.909092	1
GTTAGCG	45	1.9274012E-8	40.000004	1
GCCGATT	40	3.4570803E-7	39.375004	9
TATTACG	40	3.4570803E-7	39.375004	1
CAATACG	40	3.4570803E-7	39.375004	35
TAGTACG	40	3.4570803E-7	39.375004	1
>>END_MODULE