FastQCFastQC Report
Sat 18 Jun 2016
SRR3551356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800635
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG32860.41042422577079446No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC29580.3694567437096804No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC24970.3118774472762245No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24130.3013857750410612No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC16140.201589987947067No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8880.11091196362886957No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC8420.10516652407151825No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT8110.1012945974133032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGG302.1646738E-645.0000042
CGATCGA302.1646738E-645.00000441
CTCGTAG253.889741E-545.01
CCGATCG351.2113014E-745.040
CGTTGCG207.0320023E-445.041
TCGTGCA207.0320023E-445.036
TATAGCG207.0320023E-445.01
TTACGAG253.889741E-545.01
CCGCCTA207.0320023E-445.027
CGATATT207.0320023E-445.010
CGATAGA351.2113014E-745.010
CGAATAT1250.041.39999814
TGTTACG603.6379788E-1241.2500041
CCACGCT603.6379788E-1241.25000412
AACACGT501.0804797E-940.541
GTACGAG451.9270374E-840.01
CTACGAA1300.039.8076911
GCTACGA1300.039.8076910
TCGTTGA403.4564619E-739.37524
CTATCGT403.4564619E-739.37532