##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800635
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63971472643589	31.0	31.0	34.0	30.0	34.0
2	31.7785207991157	33.0	31.0	34.0	30.0	34.0
3	31.732715906749018	33.0	31.0	34.0	30.0	34.0
4	35.39950789061183	37.0	35.0	37.0	33.0	37.0
5	33.41839789666952	37.0	35.0	37.0	30.0	37.0
6	34.30092738888507	37.0	35.0	37.0	30.0	37.0
7	35.436628426186715	37.0	35.0	37.0	32.0	37.0
8	35.38331574312889	37.0	35.0	37.0	33.0	37.0
9	37.2980446770376	39.0	37.0	39.0	34.0	39.0
10	36.70046275768609	39.0	35.0	39.0	32.0	39.0
11	36.737424669168846	39.0	37.0	39.0	32.0	39.0
12	36.76714108176635	39.0	35.0	39.0	33.0	39.0
13	36.88671367102363	39.0	37.0	39.0	33.0	39.0
14	37.92808208484515	40.0	37.0	41.0	33.0	41.0
15	38.04840283025349	40.0	37.0	41.0	33.0	41.0
16	38.100992337332244	40.0	37.0	41.0	33.0	41.0
17	37.950682895451735	40.0	37.0	41.0	33.0	41.0
18	37.938407638936596	40.0	37.0	41.0	33.0	41.0
19	37.86341591361857	40.0	37.0	41.0	33.0	41.0
20	37.9978404641316	40.0	37.0	41.0	34.0	41.0
21	37.88359739456806	40.0	37.0	41.0	33.0	41.0
22	38.00897287777826	40.0	37.0	41.0	34.0	41.0
23	38.05484271859212	40.0	37.0	41.0	34.0	41.0
24	37.94839720971479	40.0	36.0	41.0	33.0	41.0
25	37.65892198067784	40.0	36.0	41.0	33.0	41.0
26	37.75242900947373	40.0	36.0	41.0	33.0	41.0
27	37.380993836142565	40.0	36.0	41.0	33.0	41.0
28	37.40831714826356	40.0	36.0	41.0	32.0	41.0
29	37.63962354880813	40.0	36.0	41.0	33.0	41.0
30	37.399450436216256	40.0	36.0	41.0	32.0	41.0
31	37.4253811037489	39.0	36.0	41.0	33.0	41.0
32	37.487017180113284	40.0	36.0	41.0	33.0	41.0
33	37.52093775565645	40.0	36.0	41.0	33.0	41.0
34	37.42643027097242	40.0	36.0	41.0	33.0	41.0
35	37.392709536805164	40.0	36.0	41.0	33.0	41.0
36	37.22075477589663	40.0	36.0	41.0	32.0	41.0
37	37.199813897718684	40.0	36.0	41.0	32.0	41.0
38	37.05301417000256	40.0	35.0	41.0	32.0	41.0
39	36.942959026272895	40.0	35.0	41.0	31.0	41.0
40	36.90340667095493	39.0	35.0	41.0	31.0	41.0
41	36.74611277298644	39.0	35.0	41.0	31.0	41.0
42	36.87803555927483	39.0	35.0	41.0	31.0	41.0
43	36.80714557819731	39.0	35.0	41.0	31.0	41.0
44	36.88252574518975	39.0	35.0	41.0	31.0	41.0
45	36.870640179357636	39.0	35.0	41.0	32.0	41.0
46	36.72609741018067	39.0	35.0	41.0	31.0	41.0
47	36.662370493420845	39.0	35.0	41.0	31.0	41.0
48	36.62922805023513	39.0	35.0	41.0	31.0	41.0
49	36.5728777782635	39.0	35.0	41.0	31.0	41.0
50	36.47129341085513	39.0	35.0	41.0	31.0	41.0
51	35.49982326528318	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	18.0
9	44.0
10	33.0
11	37.0
12	36.0
13	38.0
14	41.0
15	62.0
16	101.0
17	185.0
18	263.0
19	453.0
20	908.0
21	1628.0
22	2589.0
23	3317.0
24	4263.0
25	5335.0
26	6318.0
27	7091.0
28	7838.0
29	9055.0
30	11186.0
31	14870.0
32	19856.0
33	28383.0
34	49988.0
35	59675.0
36	65335.0
37	98720.0
38	167170.0
39	235734.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.265120810356784	22.197255928107065	24.651682726835574	11.885940534700582
2	31.063343471119797	26.591393081741366	25.73707119973521	16.608192247403622
3	31.72918995547284	25.64277105047868	26.576779681128105	16.05125931292037
4	28.867586353332044	25.67412116632423	27.97804242882212	17.480250051521605
5	25.70634558818937	34.425799521629706	22.904694398820936	16.963160491359982
6	25.959144928712835	36.31342621793951	23.35471219719348	14.372716656154177
7	80.6221311833732	6.06518575880395	9.496212381422247	3.8164706764006073
8	82.06611002516753	7.048904931710455	7.114852585760054	3.770132457361969
9	75.70515903001991	8.359864357666103	8.979622424700393	6.955354187613581
10	40.21920100919895	32.50382508883574	15.295609110268723	11.981364791696592
11	29.164975300854945	25.100576417468634	27.96380373078869	17.77064455088773
12	28.089204194170875	21.855277373584716	29.56141063031219	20.494107801932216
13	25.518244893116087	24.365909559287317	30.508908553835397	19.606936993761202
14	19.718473461689783	28.827368276430583	29.530685018766356	21.92347324311328
15	19.706607879995254	26.905144041916728	33.140319871102314	20.247928206985705
16	22.36574718816939	26.59663891785895	30.08262191885191	20.95499197511975
17	22.44980546691064	27.708506373066378	27.510163807477817	22.331524352545166
18	22.21511675107883	27.01405759178652	29.35370050022794	21.417125156906707
19	24.308205361993917	27.644682033635803	27.080879551855713	20.966233052514564
20	26.616123452009965	27.068639267581357	28.132794594290782	18.182442686117895
21	25.270316686130386	28.21610346787238	27.826787487431854	18.686792358565388
22	23.65572326965471	25.177265545473283	29.00884922592692	22.158161958945087
23	23.369950102106454	27.24799690245867	28.54296901834169	20.839083977093182
24	22.228730944812554	26.622743197586917	29.33633928069595	21.812186576904583
25	21.836042641153583	29.64559380991338	27.13646043453009	21.38190311440294
26	21.489317853953423	29.550294453777315	27.67865506754014	21.281732624729123
27	22.65564208409575	29.089535181449723	27.467447713377506	20.787375021077022
28	21.173943182598812	28.843480487363156	29.565282556970406	20.417293773067627
29	22.742573082615674	25.635651701461963	29.620988340504724	22.00078687541764
30	22.03438520674215	27.738732381172444	28.219225989370937	22.00765642271447
31	23.880794619270954	27.31744178058666	26.419154795880768	22.382608804261615
32	24.47956934183492	27.97979104086132	26.57503106908891	20.965608548214853
33	23.676581713265097	27.42947785195501	26.186339592948098	22.707600841831795
34	22.247466073803917	27.15806828330013	28.241957945880458	22.352507697015493
35	23.66296751953137	26.046575530672527	27.32943226314113	22.96102468665497
36	23.12227169684063	29.143867055524673	25.901190929699553	21.832670317935136
37	22.434192859417838	28.253448824995157	27.906973839514887	21.40538447607212
38	21.87451210601585	28.378599486657464	26.402043378068658	23.34484502925803
39	22.23147876373129	27.578984181306087	28.960137890549376	21.229399164413245
40	22.154040230566988	26.89789979204007	28.801014194982734	22.147045782410213
41	20.186601884753976	27.90522522747569	28.10019546984581	23.807977417924523
42	21.492440375451984	27.958308092951217	28.13379380117032	22.415457730426475
43	22.96627052277255	27.18841919226614	27.352788723950365	22.492521561010946
44	21.925346756012416	26.5331892810082	28.407951188743937	23.133512774235452
45	21.722008156026153	26.034585048118057	27.653549994691712	24.589856801164075
46	22.123439519881096	28.595177577797624	27.346668581813187	21.934714320508096
47	21.11811249820455	27.180425537229823	29.37218582749942	22.329276137066202
48	21.963441518294854	25.914930024293213	28.659126818088144	23.462501639323786
49	21.513173918202426	25.97925396716356	30.10985030631936	22.39772180831465
50	20.9245161652938	26.171601291474893	28.99648404079262	23.90739850243869
51	20.62013276961412	25.753433212387666	28.14353606824583	25.482897949752388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	237.0
1	629.0
2	1021.0
3	2831.5
4	4642.0
5	3213.5
6	1785.0
7	1764.0
8	1743.0
9	1717.5
10	1692.0
11	1683.0
12	1674.0
13	1741.5
14	1809.0
15	1854.0
16	1899.0
17	1816.5
18	1734.0
19	1896.0
20	2058.0
21	2645.5
22	3233.0
23	3435.0
24	3637.0
25	4632.5
26	7424.5
27	9221.0
28	11183.0
29	13145.0
30	15260.0
31	17375.0
32	19674.0
33	21973.0
34	25185.0
35	28397.0
36	29848.0
37	31299.0
38	33102.5
39	34906.0
40	37829.5
41	40753.0
42	44710.5
43	48668.0
44	51834.0
45	55000.0
46	63421.0
47	71842.0
48	69011.0
49	66180.0
50	63334.0
51	60488.0
52	55212.0
53	49936.0
54	46174.5
55	42413.0
56	39339.5
57	36266.0
58	34405.0
59	32544.0
60	31924.5
61	31305.0
62	27904.0
63	24503.0
64	21705.0
65	18907.0
66	15789.0
67	12671.0
68	10276.0
69	7881.0
70	6606.0
71	5331.0
72	4125.0
73	2919.0
74	2432.5
75	1383.0
76	820.0
77	771.5
78	723.0
79	512.5
80	302.0
81	199.5
82	97.0
83	56.5
84	16.0
85	13.0
86	10.0
87	6.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	800635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.076043370866344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.06969052439867	20.325901964761325
2	8.81797934683933	4.775119824768508
3	3.1392918962707794	2.5499881061171057
4	1.5967211535040895	1.7293156481382588
5	1.0092937879960926	1.366384118886409
6	0.7328750590922949	1.1906014131245526
7	0.5682128396132168	1.0769468842475407
8	0.49519607683072425	1.0726360362681242
9	0.42051787604144963	1.02473642249306
>10	7.444174672159434	49.85163225126494
>50	0.6697437693346722	10.888093914426644
>100	0.028390806065044787	1.491298750920955
>500	0.00558507660295963	1.0483449644170548
>1k	0.002327115251233179	1.6089997001655192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	3286	0.41042422577079446	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC	2958	0.3694567437096804	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC	2497	0.3118774472762245	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2413	0.3013857750410612	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1614	0.201589987947067	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	888	0.11091196362886957	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	842	0.10516652407151825	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	811	0.1012945974133032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.996034397696828E-4	0.0	0.0	0.10416731719197887	0.0
2	4.996034397696828E-4	0.0	0.0	0.5329519693743091	0.0
3	4.996034397696828E-4	0.0	0.0	0.6888282425824502	0.0
4	4.996034397696828E-4	0.0	0.0	0.958364298338194	0.0
5	4.996034397696828E-4	0.0	0.0	1.8146845941034304	0.0
6	4.996034397696828E-4	0.0	0.0	2.2527119099214996	0.0
7	4.996034397696828E-4	0.0	0.0	2.7077257426917383	0.0
8	4.996034397696828E-4	0.0	0.0	3.4496368507497173	0.0
9	4.996034397696828E-4	0.0	0.0	3.736908828617285	0.0
10	4.996034397696828E-4	0.0	0.0	4.734866699557227	0.0
11	4.996034397696828E-4	0.0	0.0	5.467535143979466	0.0
12	4.996034397696828E-4	0.0	0.0	6.371067964802938	0.0
13	4.996034397696828E-4	0.0	0.0	6.647848270435342	0.0
14	4.996034397696828E-4	0.0	0.0	6.755263009985824	0.0
15	4.996034397696828E-4	0.0	0.0	6.969093282207248	0.0
16	4.996034397696828E-4	0.0	0.0	7.330431470020671	0.0
17	4.996034397696828E-4	0.0	0.0	7.770582100457761	0.0
18	4.996034397696828E-4	0.0	0.0	8.30091115177328	0.0
19	4.996034397696828E-4	0.0	0.0	8.67598843418037	0.0
20	4.996034397696828E-4	0.0	0.0	8.976499903201834	0.0
21	4.996034397696828E-4	0.0	0.0	9.315106134505736	0.0
22	4.996034397696828E-4	0.0	0.0	9.695304352170465	0.0
23	4.996034397696828E-4	0.0	0.0	10.076002173274963	0.0
24	4.996034397696828E-4	0.0	0.0	10.372142112198443	0.0
25	4.996034397696828E-4	0.0	0.0	10.631436297438908	0.0
26	4.996034397696828E-4	0.0	0.0	10.883736034522597	0.0
27	6.245042997121036E-4	0.0	0.0	11.151398577379204	0.0
28	6.245042997121036E-4	0.0	0.0	11.422933046894029	0.0
29	6.245042997121036E-4	0.0	0.0	11.69759003790741	0.0
30	7.494051596545243E-4	0.0	0.0	12.005470657665478	0.0
31	8.743060195969449E-4	0.0	0.0	12.292118131233334	0.0
32	8.743060195969449E-4	0.0	0.0	12.57314506610378	0.0
33	8.743060195969449E-4	0.0	0.0	12.862665259450312	0.0
34	8.743060195969449E-4	0.0	0.0	13.142068483141506	0.0
35	8.743060195969449E-4	0.0	0.0	13.430464568748556	0.0
36	8.743060195969449E-4	0.0	0.0	13.71761164575618	0.0
37	8.743060195969449E-4	0.0	0.0	14.009255153721734	0.0
38	8.743060195969449E-4	0.0	0.0	14.29752633846884	0.0
39	8.743060195969449E-4	0.0	0.0	14.59516508771163	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGG	30	2.1646738E-6	45.000004	2
CGATCGA	30	2.1646738E-6	45.000004	41
CTCGTAG	25	3.889741E-5	45.0	1
CCGATCG	35	1.2113014E-7	45.0	40
CGTTGCG	20	7.0320023E-4	45.0	41
TCGTGCA	20	7.0320023E-4	45.0	36
TATAGCG	20	7.0320023E-4	45.0	1
TTACGAG	25	3.889741E-5	45.0	1
CCGCCTA	20	7.0320023E-4	45.0	27
CGATATT	20	7.0320023E-4	45.0	10
CGATAGA	35	1.2113014E-7	45.0	10
CGAATAT	125	0.0	41.399998	14
TGTTACG	60	3.6379788E-12	41.250004	1
CCACGCT	60	3.6379788E-12	41.250004	12
AACACGT	50	1.0804797E-9	40.5	41
GTACGAG	45	1.9270374E-8	40.0	1
CTACGAA	130	0.0	39.80769	11
GCTACGA	130	0.0	39.80769	10
TCGTTGA	40	3.4564619E-7	39.375	24
CTATCGT	40	3.4564619E-7	39.375	32
>>END_MODULE