FastQCFastQC Report
Sat 18 Jun 2016
SRR3551354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964400
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.31584404811281624No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC25490.26430941518042306No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC22440.23268353380340107No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT17200.1783492326835338Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11740.12173372044794692No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC11170.11582330982994608No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10840.11240149315636665No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC9660.10016590626296142No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT253.8903367E-545.00000410
TAGGCCG302.1651376E-645.0000041
CGCATCC207.0327224E-445.041
CCGGACA207.0327224E-445.030
CGACACT207.0327224E-445.014
CGAGATT207.0327224E-445.016
AACTCGT207.0327224E-445.012
ACCCGGT207.0327224E-445.012
CGGTATT207.0327224E-445.043
GATTCGC207.0327224E-445.038
CTTACCG207.0327224E-445.033
TGTTACG850.042.352941
CGCACGG451.927765E-840.02
TAATGCG451.927765E-840.01
AATCGGC451.927765E-840.020
CTAACGG1200.039.3750042
ACGGGTC1050.038.571435
GCGATAT356.2472445E-638.5714269
CGTTTTT18850.038.5543751
CGAATAT1650.038.18181614