##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	964400
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.698355454168397	33.0	31.0	34.0	30.0	34.0
2	31.82425238490253	33.0	31.0	34.0	30.0	34.0
3	31.77178763998341	33.0	31.0	34.0	30.0	34.0
4	35.47333575279967	37.0	35.0	37.0	33.0	37.0
5	33.56755080879303	37.0	35.0	37.0	30.0	37.0
6	34.41684155951887	37.0	35.0	37.0	30.0	37.0
7	35.5067015761095	37.0	35.0	37.0	32.0	37.0
8	35.47888324346744	37.0	35.0	37.0	33.0	37.0
9	37.38740356698465	39.0	37.0	39.0	34.0	39.0
10	36.77896412277063	39.0	37.0	39.0	32.0	39.0
11	36.829089589382	39.0	37.0	39.0	33.0	39.0
12	36.8616849854832	39.0	37.0	39.0	33.0	39.0
13	36.99275715470759	39.0	37.0	39.0	33.0	39.0
14	38.01870489423476	40.0	37.0	41.0	33.0	41.0
15	38.14844877644131	40.0	37.0	41.0	33.0	41.0
16	38.17100373289092	40.0	37.0	41.0	34.0	41.0
17	38.07785773537951	40.0	37.0	41.0	34.0	41.0
18	38.04274263790958	40.0	37.0	41.0	33.0	41.0
19	37.96166424720033	40.0	37.0	41.0	34.0	41.0
20	38.17142472003318	40.0	37.0	41.0	34.0	41.0
21	38.00882725010369	40.0	37.0	41.0	34.0	41.0
22	38.157442969722105	40.0	37.0	41.0	34.0	41.0
23	38.25388013272501	40.0	37.0	41.0	34.0	41.0
24	38.09999896308586	40.0	37.0	41.0	34.0	41.0
25	37.74746370800498	40.0	36.0	41.0	33.0	41.0
26	37.882056200746575	40.0	37.0	41.0	33.0	41.0
27	37.58131273330568	40.0	36.0	41.0	33.0	41.0
28	37.635018664454584	40.0	36.0	41.0	33.0	41.0
29	37.86649108253837	40.0	36.0	41.0	33.0	41.0
30	37.57734861053505	40.0	36.0	41.0	33.0	41.0
31	37.51180630443799	40.0	36.0	41.0	33.0	41.0
32	37.65096951472418	40.0	36.0	41.0	33.0	41.0
33	37.678899834093734	40.0	36.0	41.0	33.0	41.0
34	37.55519805060141	40.0	36.0	41.0	33.0	41.0
35	37.54639257569473	40.0	36.0	41.0	33.0	41.0
36	37.31933119037744	40.0	36.0	41.0	33.0	41.0
37	37.30675756947325	40.0	36.0	41.0	33.0	41.0
38	37.18768871837412	40.0	35.0	41.0	32.0	41.0
39	37.10956553297387	39.0	35.0	41.0	32.0	41.0
40	36.9989133139776	39.0	35.0	41.0	32.0	41.0
41	36.8309746992949	39.0	35.0	41.0	31.0	41.0
42	37.05508606387391	39.0	35.0	41.0	32.0	41.0
43	36.994288676897554	39.0	35.0	41.0	32.0	41.0
44	37.061514931563664	39.0	35.0	41.0	32.0	41.0
45	37.04799460804645	39.0	35.0	41.0	32.0	41.0
46	36.90972003318125	39.0	35.0	41.0	32.0	41.0
47	36.86033284944006	39.0	35.0	41.0	32.0	41.0
48	36.83033907092493	39.0	35.0	41.0	32.0	41.0
49	36.7819732476151	39.0	35.0	41.0	31.0	41.0
50	36.7178836582331	39.0	35.0	41.0	31.0	41.0
51	35.76625881377022	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	32.0
9	51.0
10	50.0
11	51.0
12	33.0
13	40.0
14	57.0
15	72.0
16	97.0
17	160.0
18	274.0
19	484.0
20	756.0
21	1162.0
22	1799.0
23	2495.0
24	3664.0
25	5114.0
26	6875.0
27	8172.0
28	9388.0
29	10721.0
30	13165.0
31	17329.0
32	23310.0
33	33560.0
34	59415.0
35	72280.0
36	80033.0
37	120145.0
38	209135.0
39	284388.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.1395686437163	22.990875155537122	25.905226047283286	13.964330153463292
2	30.070510161758605	25.460182496889256	27.35866860223973	17.110638739112403
3	32.024471173786814	23.99077146412277	28.436229780174198	15.548527581916217
4	29.47573620904189	25.939236831190378	26.485483201990874	18.099543757776857
5	24.769182911654916	33.72324761509747	24.113542098714227	17.394027374533387
6	26.143716300290336	34.01150974699295	24.26876814599751	15.576005806719204
7	80.60338034010785	5.952198257984239	8.859083367897139	4.585338034010784
8	82.25881377021983	5.403774367482372	7.961012028204065	4.3763998340937365
9	75.04043965159684	8.578287017834924	10.41974284529241	5.961530485275819
10	39.03090004147657	31.376088759850685	17.04313562836997	12.54987557030278
11	27.909581086686025	25.706345914558277	27.544483616756533	18.83958938199917
12	27.16528411447532	22.33513065118208	30.01503525508088	20.48454997926172
13	24.415491497304025	24.884280381584407	30.630754873496475	20.069473247615097
14	19.364371630029034	28.13759850684363	29.963396930734138	22.534632932393198
15	19.41642472003318	26.626503525508088	33.35120282040647	20.60586893405226
16	21.652944836167563	26.616756532559105	31.26762754043965	20.462671090833677
17	21.500725839900454	26.755910410618	28.54106180008295	23.202301949398592
18	21.71153048527582	27.035462463708004	29.966715055993365	21.286291995022815
19	22.91124014931564	27.58378266279552	27.96640398175031	21.53857320613853
20	24.816569888013273	27.174097884695147	28.786603069265865	19.222729158025714
21	24.49294898382414	26.635939444214017	28.965055993363748	19.906055578598092
22	22.65367067606802	25.32051016175861	28.97355868934052	23.05226047283285
23	21.977187888842803	25.99637080049772	29.24554126918291	22.780900041476563
24	22.09912899211945	25.92710493571132	30.11520116134384	21.858564910825386
25	20.99180837826628	28.24201576109498	28.046038987971794	22.720136872666945
26	20.265346329323933	28.898278722521773	27.89070924927416	22.945665698880134
27	21.21163417669017	29.38593944421402	28.134176690170055	21.26824968892576
28	19.50694732476151	28.11146827042721	29.778307756117794	22.603276648693488
29	21.62702198257984	25.913625051845706	28.680734135213605	23.778618830360845
30	22.578805474906677	27.375673994193278	27.497718788884278	22.54780174201576
31	22.287121526337618	27.875777685607634	26.4592492741601	23.377851513894647
32	23.173475736209042	27.886665284114475	26.331397760265453	22.608461219411033
33	23.183430111986727	27.59788469514724	27.097055163832433	22.121630029033597
34	21.498029863127332	26.313562836997097	28.623496474491912	23.56491082538366
35	23.344566569888016	25.404292824554126	27.596951472418084	23.654189133139774
36	22.706656988801328	27.486727498963088	27.786603069265865	22.020012442969723
37	22.529655744504353	27.402841144753214	28.91528411447532	21.15221899626711
38	22.729780174201576	27.065947739527168	27.4765657403567	22.72770634591456
39	23.982994608046454	25.24377851513895	29.186955620074656	21.58627125673994
40	23.877229365408546	25.279448361675655	29.51192451265035	21.33139776026545
41	20.923994193280794	26.045831605143093	29.60783907092493	23.42233513065118
42	21.666321028618828	26.241082538365823	28.920883450850273	23.171712982165076
43	22.824761509746992	25.568540024885937	29.104002488593945	22.502695976773126
44	21.98372044794691	26.34674408958938	28.041476565740357	23.62805889672335
45	22.518871837411865	25.92700124429697	27.156574035669845	24.39755288262132
46	22.321858150145168	26.754769805060143	27.729780174201572	23.193591870593114
47	21.2326835338034	25.599854832019908	30.318954790543344	22.84850684363335
48	21.479883865615925	25.02561177934467	29.510680215678143	23.98382413936126
49	21.73714226462049	25.01150974699295	30.35338034010784	22.897967648278723
50	20.992741600995437	24.722729158025718	30.18716300290336	24.097366238075487
51	21.063355454168395	24.45831605143094	28.373496474491912	26.104832019908752
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	350.0
1	872.5
2	1395.0
3	3607.5
4	5820.0
5	4109.0
6	2398.0
7	2320.0
8	2242.0
9	2260.0
10	2278.0
11	2312.0
12	2346.0
13	2368.0
14	2390.0
15	2447.0
16	2504.0
17	2452.0
18	2400.0
19	2762.5
20	3125.0
21	3545.5
22	3966.0
23	4397.5
24	4829.0
25	6295.0
26	9815.0
27	11869.0
28	13656.0
29	15443.0
30	18235.5
31	21028.0
32	23750.0
33	26472.0
34	28842.5
35	31213.0
36	33371.0
37	35529.0
38	37697.0
39	39865.0
40	42970.0
41	46075.0
42	49113.0
43	52151.0
44	57441.5
45	62732.0
46	64917.0
47	67102.0
48	73279.0
49	79456.0
50	82006.5
51	84557.0
52	82085.0
53	79613.0
54	69482.5
55	59352.0
56	53274.0
57	47196.0
58	43048.5
59	38901.0
60	37750.0
61	36599.0
62	32824.0
63	29049.0
64	24885.5
65	20722.0
66	16609.5
67	12497.0
68	10679.0
69	8861.0
70	7352.5
71	5844.0
72	4715.5
73	3587.0
74	2944.5
75	1764.0
76	1226.0
77	1016.0
78	806.0
79	555.0
80	304.0
81	215.0
82	126.0
83	82.5
84	39.0
85	43.5
86	48.0
87	28.0
88	8.0
89	9.0
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	4.5
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	964400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.439047463183723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33120453180638	20.395774491438523
2	10.218755701942845	5.607858454405781
3	3.6594459135230912	3.012351303303415
4	1.7944522858000966	1.9695224576194954
5	1.039609659214295	1.4262949391182647
6	0.7053513177685099	1.1612500971881596
7	0.49923709602791144	0.9589013261304312
8	0.40004757374456773	0.8781539490806949
9	0.3801643231964061	0.9388212217195774
>10	5.886981116563664	41.579543644150554
>50	1.0241456100960267	17.480866711412553
>100	0.05370051800175299	2.403895418695455
>500	0.004219326414423447	0.8254707429434521
>1k	0.002685025900087648	1.3612952427936518
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3046	0.31584404811281624	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	2549	0.26430941518042306	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	2244	0.23268353380340107	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	1720	0.1783492326835338	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1174	0.12173372044794692	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	1117	0.11582330982994608	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1084	0.11240149315636665	No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	966	0.10016590626296142	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18799253421816672	0.0
2	0.0	0.0	0.0	0.8900871007880548	0.0
3	0.0	0.0	0.0	1.1518042306097056	0.0
4	0.0	0.0	0.0	1.4907714641227707	0.0
5	0.0	0.0	0.0	2.468477810037329	0.0
6	0.0	0.0	0.0	3.118726669431771	0.0
7	0.0	0.0	0.0	3.7718788884280383	0.0
8	0.0	0.0	0.0	4.746267109083368	0.0
9	0.0	0.0	0.0	5.160203235172128	0.0
10	1.0369141435089174E-4	0.0	0.0	6.070095396101203	0.0
11	1.0369141435089174E-4	0.0	0.0	7.207590211530485	0.0
12	1.0369141435089174E-4	0.0	0.0	8.187059311489008	0.0
13	1.0369141435089174E-4	0.0	0.0	8.593737038573206	0.0
14	1.0369141435089174E-4	0.0	0.0	8.772812111157196	0.0
15	1.0369141435089174E-4	0.0	0.0	9.047490667772708	0.0
16	1.0369141435089174E-4	0.0	0.0	9.542928245541269	0.0
17	1.0369141435089174E-4	0.0	0.0	10.162691829116548	0.0
18	1.0369141435089174E-4	0.0	0.0	10.84187059311489	0.0
19	1.0369141435089174E-4	0.0	0.0	11.26275404396516	0.0
20	1.0369141435089174E-4	0.0	0.0	11.663625051845708	0.0
21	1.0369141435089174E-4	0.0	0.0	12.154292824554126	0.0
22	1.0369141435089174E-4	0.0	0.0	12.687059311489008	0.0
23	1.0369141435089174E-4	0.0	0.0	13.21858150145168	0.0
24	1.0369141435089174E-4	0.0	0.0	13.62919950228121	0.0
25	1.0369141435089174E-4	0.0	0.0	14.010991289921195	0.0
26	1.0369141435089174E-4	0.0	0.0	14.344670261302364	0.0
27	1.0369141435089174E-4	0.0	0.0	14.691103276648693	0.0
28	1.0369141435089174E-4	0.0	0.0	15.035151389464952	0.0
29	1.0369141435089174E-4	0.0	0.0	15.420572376607216	0.0
30	1.0369141435089174E-4	0.0	0.0	15.840315221899626	0.0
31	2.0738282870178348E-4	0.0	0.0	16.236934881791786	0.0
32	2.0738282870178348E-4	0.0	0.0	16.63137702198258	0.0
33	2.0738282870178348E-4	0.0	0.0	17.001347988386563	0.0
34	2.0738282870178348E-4	0.0	0.0	17.380651182082122	0.0
35	2.0738282870178348E-4	0.0	0.0	17.766798009124845	0.0
36	2.0738282870178348E-4	0.0	0.0	18.148589796764828	0.0
37	2.0738282870178348E-4	0.0	0.0	18.53255910410618	0.0
38	2.0738282870178348E-4	0.0	0.0	18.89765657403567	0.0
39	3.1107424305267526E-4	0.0	0.0	19.28815844048113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTGT	25	3.8903367E-5	45.000004	10
TAGGCCG	30	2.1651376E-6	45.000004	1
CGCATCC	20	7.0327224E-4	45.0	41
CCGGACA	20	7.0327224E-4	45.0	30
CGACACT	20	7.0327224E-4	45.0	14
CGAGATT	20	7.0327224E-4	45.0	16
AACTCGT	20	7.0327224E-4	45.0	12
ACCCGGT	20	7.0327224E-4	45.0	12
CGGTATT	20	7.0327224E-4	45.0	43
GATTCGC	20	7.0327224E-4	45.0	38
CTTACCG	20	7.0327224E-4	45.0	33
TGTTACG	85	0.0	42.35294	1
CGCACGG	45	1.927765E-8	40.0	2
TAATGCG	45	1.927765E-8	40.0	1
AATCGGC	45	1.927765E-8	40.0	20
CTAACGG	120	0.0	39.375004	2
ACGGGTC	105	0.0	38.57143	5
GCGATAT	35	6.2472445E-6	38.571426	9
CGTTTTT	1885	0.0	38.554375	1
CGAATAT	165	0.0	38.181816	14
>>END_MODULE