##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551353_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	656912
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68112928367879	33.0	31.0	34.0	30.0	34.0
2	31.820000243563825	33.0	31.0	34.0	30.0	34.0
3	31.75988564678374	33.0	31.0	34.0	30.0	34.0
4	35.44262397398738	37.0	35.0	37.0	33.0	37.0
5	33.447177095257814	37.0	35.0	37.0	30.0	37.0
6	34.3449990257447	37.0	35.0	37.0	30.0	37.0
7	35.45294499110992	37.0	35.0	37.0	32.0	37.0
8	35.39810202888667	37.0	35.0	37.0	33.0	37.0
9	37.33306439827557	39.0	37.0	39.0	34.0	39.0
10	36.747926662932024	39.0	35.0	39.0	32.0	39.0
11	36.7920086708722	39.0	37.0	39.0	33.0	39.0
12	36.75178410502472	39.0	35.0	39.0	33.0	39.0
13	36.82852649974426	39.0	37.0	39.0	33.0	39.0
14	37.81473317582873	40.0	37.0	41.0	33.0	41.0
15	37.96471216844874	40.0	37.0	41.0	33.0	41.0
16	38.01260747253818	40.0	37.0	41.0	33.0	41.0
17	37.91266257885379	40.0	36.0	41.0	33.0	41.0
18	37.8821257641815	39.0	36.0	41.0	33.0	41.0
19	37.78044091141584	39.0	36.0	41.0	33.0	41.0
20	37.975336118079746	40.0	36.0	41.0	34.0	41.0
21	37.812050929196	39.0	36.0	41.0	33.0	41.0
22	37.95600171712497	40.0	36.0	41.0	34.0	41.0
23	38.0433802396668	40.0	36.0	41.0	34.0	41.0
24	37.89651277492267	40.0	36.0	41.0	34.0	41.0
25	37.57416670726064	39.0	35.0	41.0	33.0	41.0
26	37.69055672601505	39.0	35.0	41.0	33.0	41.0
27	37.33673307840319	40.0	35.0	41.0	33.0	41.0
28	37.42443736756217	40.0	35.0	41.0	33.0	41.0
29	37.645587232384244	40.0	35.0	41.0	33.0	41.0
30	37.36925189370875	39.0	35.0	41.0	33.0	41.0
31	37.338483693401855	39.0	35.0	41.0	33.0	41.0
32	37.41167157853715	39.0	35.0	41.0	33.0	41.0
33	37.416583956450786	40.0	35.0	41.0	33.0	41.0
34	37.29982098058796	40.0	35.0	41.0	33.0	41.0
35	37.269760637650094	40.0	35.0	41.0	33.0	41.0
36	37.04206042818521	39.0	35.0	41.0	32.0	41.0
37	37.02239873833938	39.0	35.0	41.0	32.0	41.0
38	36.85670987894878	39.0	35.0	41.0	32.0	41.0
39	36.77944534427747	39.0	35.0	41.0	31.0	41.0
40	36.67078999926931	39.0	35.0	41.0	31.0	41.0
41	36.561012738388094	39.0	35.0	41.0	31.0	41.0
42	36.706709574494	39.0	35.0	41.0	31.0	41.0
43	36.61887741432643	39.0	35.0	41.0	31.0	41.0
44	36.64713081813089	39.0	35.0	41.0	31.0	41.0
45	36.65986007258202	39.0	35.0	41.0	31.0	41.0
46	36.53317643763548	39.0	35.0	41.0	31.0	41.0
47	36.519345056872154	39.0	35.0	41.0	31.0	41.0
48	36.462271963368	39.0	35.0	41.0	31.0	41.0
49	36.41460043354361	39.0	35.0	41.0	31.0	41.0
50	36.31296733809095	38.0	35.0	41.0	31.0	41.0
51	35.416281023942325	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	20.0
9	27.0
10	27.0
11	39.0
12	24.0
13	43.0
14	33.0
15	64.0
16	82.0
17	125.0
18	200.0
19	356.0
20	557.0
21	823.0
22	1268.0
23	1991.0
24	2852.0
25	4101.0
26	5250.0
27	6582.0
28	7131.0
29	8113.0
30	9826.0
31	12406.0
32	16805.0
33	25105.0
34	46248.0
35	56530.0
36	54920.0
37	80307.0
38	134076.0
39	180915.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.56435565189858	22.487030226270793	24.97716589132182	12.971448230508805
2	33.334906349708945	24.738321114548068	24.79631975059064	17.13045278515235
3	32.7674026353606	23.513803979832915	28.023540443773292	15.695252941033196
4	29.845550088900797	25.409491682295343	26.546173612295103	18.198784616508757
5	24.66966656112234	34.32286211851816	23.790553377012447	17.216917943347056
6	26.308394427259664	34.89447597242857	24.465986311712985	14.331143288598778
7	78.87677497138125	7.218013980563606	9.567795990939427	4.337415057115717
8	80.12701853520716	6.021202231044645	9.276584991597048	4.575194242151156
9	73.39674111600945	8.938183500986433	11.864450641790683	5.8006247412134355
10	42.538269966144625	25.395334534914877	18.3499464159583	13.716449082982196
11	31.83226977129357	25.670561658182528	24.887960640085733	17.609207930438174
12	29.689973695106804	23.292769807828144	27.76673283483937	19.250523662225685
13	25.29943127846652	25.759310227245045	29.271043914557808	19.67021457973062
14	20.81131110407482	28.281718099227902	29.341220741895413	21.56575005480186
15	20.990634970894124	25.95066005796819	33.72399956158511	19.334705409552573
16	23.528874491560515	25.317241883235504	31.46494507635726	19.688938548846725
17	23.567083566748668	25.421213191416808	28.29191733443749	22.719785907397032
18	23.315451689115132	26.296825145529386	29.888934895389337	20.498788269966145
19	25.592925688676715	26.490610614511535	27.276408407823272	20.640055288988478
20	26.521208320140293	27.24702852132401	27.53458606327788	18.697177095257814
21	25.350427454514456	27.677375356212096	28.171962150181457	18.800235039091994
22	23.014802591519107	25.605865016927687	27.43061475509657	23.948717636456635
23	22.523869254938255	26.44235453150498	29.000992522590547	22.03278369096622
24	22.68096792264413	25.44556957400687	30.338919063740654	21.53454343960835
25	22.248489904279417	26.630507587013174	28.17592006235234	22.945082446355066
26	21.218976057675913	28.0535292398373	28.423898482597366	22.30359621988942
27	21.455080741408285	28.645846018949268	29.475637528314298	20.423435711328153
28	20.910867817911683	27.10530482012811	30.636219158730544	21.347608203229658
29	21.775367172467543	26.410082324573153	29.352485568843317	22.462064934115986
30	22.49509827800375	27.445533039432984	28.491182989502402	21.56818569306087
31	23.85159656087878	26.978651630659815	26.842712570329052	22.32703923813235
32	24.766787636700197	27.557115717173687	26.853977397276957	20.822119248849162
33	24.61790924811847	27.69518596098107	26.62457071875685	21.062334072143603
34	22.1834888082422	27.453144408992376	27.996900650315414	22.366466132450007
35	24.2839223518523	25.9442665075383	27.713301020532434	22.058510120076967
36	23.741383929658767	28.521324012957592	27.19176997832282	20.545522079060817
37	23.05164162018657	28.606571352022797	28.9714604087001	19.370326619090534
38	24.07476191636018	28.383862678714955	26.81287600165623	20.72849940326863
39	24.195326010180967	27.092822174050713	26.92141413157318	21.790437684195144
40	24.065171590715348	26.666585478724702	27.96797744598972	21.300265484570232
41	22.206627371702755	26.450574810629124	27.421633339016488	23.92116447865163
42	22.84872250773315	25.63037362690893	28.182618067564608	23.33828579779331
43	23.725400053584043	24.524289402537935	28.30531334486202	23.444997199016
44	21.770495895949534	27.067552427113522	27.908760990817644	23.2531906861193
45	22.686904790900456	26.531864237523443	27.467758238546413	23.31347273302969
46	22.14725868913949	28.27030104488881	26.8509328494532	22.731507416518497
47	22.245597583846845	26.02844825486519	29.50258177655455	22.22337238473342
48	22.54137540492486	25.258634337628177	29.146826363348517	23.05316389409845
49	23.119535036656355	25.00197895608544	29.93977884404608	21.938707163212122
50	21.926224517134717	26.120393599142655	28.88255961224639	23.070822271476242
51	21.977525147965025	25.49808802396668	27.173046009206715	25.351340818861583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	778.5
2	1283.0
3	3648.5
4	6014.0
5	4108.5
6	2203.0
7	2171.5
8	2140.0
9	2041.0
10	1942.0
11	2037.5
12	2133.0
13	2078.0
14	2023.0
15	1957.0
16	1891.0
17	1842.0
18	1793.0
19	1900.5
20	2008.0
21	3470.5
22	4933.0
23	4620.5
24	4308.0
25	5053.5
26	6497.5
27	7196.0
28	8543.0
29	9890.0
30	11381.5
31	12873.0
32	14351.0
33	15829.0
34	16884.5
35	17940.0
36	18595.0
37	19250.0
38	21870.0
39	24490.0
40	27344.0
41	30198.0
42	33339.0
43	36480.0
44	39495.0
45	42510.0
46	46532.0
47	50554.0
48	54436.5
49	58319.0
50	55676.0
51	53033.0
52	48084.5
53	43136.0
54	38468.0
55	33800.0
56	32034.5
57	30269.0
58	29700.0
59	29131.0
60	27649.5
61	26168.0
62	24250.5
63	22333.0
64	19584.0
65	16835.0
66	14278.0
67	11721.0
68	9842.0
69	7963.0
70	7007.0
71	6051.0
72	5524.5
73	4998.0
74	3858.0
75	2321.0
76	1924.0
77	1720.0
78	1516.0
79	1089.5
80	663.0
81	459.0
82	255.0
83	175.0
84	95.0
85	55.0
86	15.0
87	9.5
88	4.0
89	3.5
90	3.0
91	3.0
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	656912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.757598260932156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50536490917436	21.42595352342188
2	10.09234403600861	5.8046315059730045
3	3.5935094008135513	3.100220990864374
4	1.7980635762576556	2.0683195989453047
5	1.032775598272853	1.4850072874412281
6	0.7037986104936288	1.2143734618306827
7	0.5515866966777626	1.1103616040393618
8	0.4431329850442154	1.0194752288055362
9	0.40282386404731474	1.042582216697013
>10	5.998255778640398	42.61867993158601
>50	0.8258900866977694	14.742313240300836
>100	0.04656671260058524	2.3866059011741636
>500	0.0037467469908516854	0.840276144115431
>1k	0.0021409982804866776	1.14119936480524
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3411	0.5192476313417931	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1435	0.2184463063545802	Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1361	0.20718147940667853	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	1207	0.18373846116374798	No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	931	0.1417237011958984	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	910	0.13852692598095329	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	881	0.13411233163650535	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	793	0.1207163212119736	Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	698	0.10625471904912683	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14933507075529143	0.0
2	0.0	0.0	0.0	0.6796953016537983	0.0
3	0.0	0.0	0.0	0.9056007501765838	0.0
4	0.0	0.0	0.0	1.2140134447231896	0.0
5	0.0	0.0	0.0	2.057809874077502	0.0
6	0.0	0.0	0.0	2.6106997588718124	0.0
7	0.0	0.0	0.0	3.182618067564605	0.0
8	0.0	0.0	0.0	4.165398105073434	0.0
9	0.0	0.0	0.0	4.581131110407482	0.0
10	0.0	0.0	0.0	5.4678556640767715	0.0
11	0.0	0.0	0.0	6.661775093163163	0.0
12	0.0	0.0	0.0	7.582446355067345	0.0
13	0.0	0.0	0.0	7.945508683050393	0.0
14	0.0	0.0	0.0	8.096974937282315	0.0
15	0.0	0.0	0.0	8.356065957084054	0.0
16	0.0	0.0	0.0	8.883077185376427	0.0
17	0.0	0.0	0.0	9.464281364931681	0.0
18	0.0	0.0	0.0	10.169703095696228	1.5222739118786078E-4
19	0.0	0.0	0.0	10.590459604939474	1.5222739118786078E-4
20	0.0	0.0	0.0	11.011216114182721	1.5222739118786078E-4
21	0.0	0.0	0.0	11.501236086416446	1.5222739118786078E-4
22	0.0	0.0	0.0	12.004347614292325	1.5222739118786078E-4
23	1.5222739118786078E-4	0.0	0.0	12.503349002606132	1.5222739118786078E-4
24	1.5222739118786078E-4	0.0	0.0	12.906294907080401	1.5222739118786078E-4
25	1.5222739118786078E-4	0.0	0.0	13.27209732810483	1.5222739118786078E-4
26	1.5222739118786078E-4	0.0	0.0	13.605931996979809	1.5222739118786078E-4
27	1.5222739118786078E-4	0.0	0.0	13.921042696738681	1.5222739118786078E-4
28	1.5222739118786078E-4	0.0	0.0	14.267360011691064	1.5222739118786078E-4
29	1.5222739118786078E-4	0.0	0.0	14.622810970114719	1.5222739118786078E-4
30	1.5222739118786078E-4	0.0	0.0	15.01768882285603	1.5222739118786078E-4
31	1.5222739118786078E-4	0.0	0.0	15.388210293007283	1.5222739118786078E-4
32	1.5222739118786078E-4	0.0	0.0	15.76482085880605	1.5222739118786078E-4
33	1.5222739118786078E-4	0.0	0.0	16.10763694376111	1.5222739118786078E-4
34	1.5222739118786078E-4	0.0	0.0	16.471917090873664	1.5222739118786078E-4
35	3.0445478237572156E-4	0.0	0.0	16.84426528971917	1.5222739118786078E-4
36	3.0445478237572156E-4	0.0	0.0	17.215547896826365	1.5222739118786078E-4
37	3.0445478237572156E-4	0.0	0.0	17.59565969262245	1.5222739118786078E-4
38	3.0445478237572156E-4	0.0	0.0	17.952632924957985	1.5222739118786078E-4
39	3.0445478237572156E-4	0.0	0.0	18.33655040553377	1.5222739118786078E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGAA	20	7.0310757E-4	45.000004	24
TAACCGT	20	7.0310757E-4	45.000004	26
CGTAGCG	20	7.0310757E-4	45.000004	42
CCACGCG	20	7.0310757E-4	45.000004	14
ATCGCTA	20	7.0310757E-4	45.000004	19
ACGACAC	20	7.0310757E-4	45.000004	28
TCGCAAC	25	3.888975E-5	45.0	15
TGACCGC	25	3.888975E-5	45.0	31
TCCGTCC	25	3.888975E-5	45.0	35
TTAATCG	30	2.1640772E-6	44.999996	20
TAATCGT	30	2.1640772E-6	44.999996	21
TGCAACG	50	1.0804797E-9	40.5	1
CGTTAGG	95	0.0	40.26316	2
CGTTTTT	1835	0.0	40.095367	1
ATAGCGG	90	0.0	40.000004	2
ACTACGG	45	1.9261279E-8	40.000004	2
GGTATAC	40	3.4552068E-7	39.375004	18
CGTTATT	105	0.0	38.57143	1
CGTTGAT	35	6.244203E-6	38.571426	25
CGCGACC	70	0.0	38.571426	10
>>END_MODULE