FastQCFastQC Report
Sat 18 Jun 2016
SRR3551351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544720
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG70051.2859817888089293No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC56741.0416360699074754No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC55221.0137318255250403No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32160.5903950653546777No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC31640.5808488764870025No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCT14280.26215303275077106No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTACGT14100.2588485827581143No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTC9990.18339697459245116No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC9950.18266265237186077No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTA9880.18137758848582758No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT9820.180276105154942No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT9380.17219856072844764No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC9080.1666911440740197No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7660.14062270524306067No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC7200.13217799970627112No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT6850.12575268027610514No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6520.11969452195623441No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6310.11583933029813483No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6120.11235129975033045No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC5810.10666030254075488No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTT5510.10115288588632691No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG302.1633914E-645.0000042
TCTATTG302.1633914E-645.00000418
AGACTTA351.2103555E-745.020
CGGGTAT406.8030204E-945.06
AATTTCG207.0300145E-445.039
ATTAGCG207.0300145E-445.01
TTAATCG351.2103555E-745.020
CGTTGAT351.2103555E-745.025
ACCGAAT207.0300145E-445.039
TACGGGT253.888095E-545.04
CGACACG207.0300145E-445.040
ACGGGTA406.8030204E-945.05
TCGATAG406.8030204E-945.01
CTGAACG253.888095E-545.034
CCCCGTA207.0300145E-445.040
TACGCCT253.888095E-545.029
TACGATA207.0300145E-445.01
TGTTCGT207.0300145E-445.033
ACAGCGA253.888095E-545.045
CGAATGT207.0300145E-445.045