##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544720
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.549708106917315	31.0	31.0	34.0	30.0	34.0
2	31.70176604494052	31.0	31.0	34.0	30.0	34.0
3	31.674631003084155	31.0	31.0	34.0	30.0	34.0
4	35.40613159054193	37.0	35.0	37.0	33.0	37.0
5	33.34667902775738	37.0	35.0	37.0	30.0	37.0
6	34.24909311205757	37.0	35.0	37.0	30.0	37.0
7	35.31835438390366	37.0	35.0	37.0	32.0	37.0
8	35.05865582317521	37.0	35.0	37.0	32.0	37.0
9	36.83889704802467	39.0	37.0	39.0	32.0	39.0
10	36.53144000587458	38.0	35.0	39.0	32.0	39.0
11	36.65500991334998	39.0	35.0	39.0	32.0	39.0
12	36.59306249082097	39.0	35.0	39.0	32.0	39.0
13	36.58863085621971	39.0	35.0	39.0	32.0	39.0
14	37.57104567484212	39.0	36.0	41.0	33.0	41.0
15	37.68055331179321	39.0	36.0	41.0	33.0	41.0
16	37.82712402702306	39.0	36.0	41.0	33.0	41.0
17	37.61221728594507	39.0	36.0	41.0	33.0	41.0
18	37.64507453370539	39.0	36.0	41.0	33.0	41.0
19	37.541057791158764	39.0	36.0	41.0	33.0	41.0
20	37.53082133940373	39.0	35.0	41.0	33.0	41.0
21	37.504953003377885	39.0	35.0	41.0	33.0	41.0
22	37.59265494198854	39.0	35.0	41.0	33.0	41.0
23	37.592998237626674	39.0	35.0	41.0	33.0	41.0
24	37.50476391540608	39.0	35.0	41.0	33.0	41.0
25	37.2858239095315	39.0	35.0	41.0	32.0	41.0
26	37.321484432368926	39.0	35.0	41.0	32.0	41.0
27	36.870964899397855	39.0	35.0	41.0	32.0	41.0
28	36.91312784549861	39.0	35.0	41.0	31.0	41.0
29	37.19753267733881	39.0	35.0	41.0	32.0	41.0
30	36.93751468644441	39.0	35.0	41.0	31.0	41.0
31	36.937072257306504	39.0	35.0	41.0	31.0	41.0
32	36.84667719195183	39.0	35.0	41.0	31.0	41.0
33	36.82794463210457	39.0	35.0	41.0	31.0	41.0
34	36.71802761051549	39.0	35.0	41.0	31.0	41.0
35	36.640938463797916	39.0	35.0	41.0	31.0	41.0
36	36.44589330298135	39.0	35.0	41.0	30.0	41.0
37	36.41720333382288	39.0	35.0	41.0	30.0	41.0
38	36.274443750917904	39.0	35.0	41.0	30.0	41.0
39	36.21184645322367	39.0	35.0	41.0	30.0	41.0
40	36.14128910265825	39.0	35.0	41.0	30.0	41.0
41	35.94716000881186	39.0	35.0	40.0	29.0	41.0
42	36.10165406080188	39.0	35.0	41.0	30.0	41.0
43	35.98366683800852	39.0	35.0	41.0	30.0	41.0
44	36.1278601850492	39.0	35.0	41.0	30.0	41.0
45	36.13061756498752	39.0	35.0	41.0	30.0	41.0
46	36.016072477603174	39.0	35.0	41.0	30.0	41.0
47	35.901086796886474	38.0	35.0	41.0	30.0	41.0
48	35.930542296959906	38.0	35.0	40.0	30.0	41.0
49	35.895524306065504	38.0	35.0	40.0	29.0	41.0
50	35.77934902335144	38.0	35.0	40.0	29.0	41.0
51	34.96276252019386	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	9.0
9	28.0
10	16.0
11	28.0
12	28.0
13	35.0
14	49.0
15	55.0
16	70.0
17	116.0
18	256.0
19	486.0
20	1123.0
21	1866.0
22	2646.0
23	3415.0
24	4143.0
25	5083.0
26	5831.0
27	6263.0
28	6524.0
29	7331.0
30	8797.0
31	11657.0
32	15630.0
33	22233.0
34	38669.0
35	46444.0
36	45512.0
37	63931.0
38	105492.0
39	140916.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.2191584667352	19.148553385225437	21.376670583051844	13.255617564987515
2	30.955903950653546	29.967506241738874	22.547180202672934	16.529409604934646
3	30.67539286238802	28.395689528565132	25.569834043178147	15.359083565868703
4	27.33459391981201	23.504919958877956	31.414855338522546	17.745630782787487
5	27.653289763548244	32.182405639594656	23.153363195770304	17.010941401086797
6	24.90600675576443	37.37204435306212	24.29890585989132	13.423043031282125
7	72.09410339256866	6.743097371126449	16.513988838302247	4.648810398002643
8	71.58852254369216	12.362681744749596	10.656484065207813	5.392311646350419
9	65.41415773241297	8.92733881627258	12.916911440740197	12.74159201057424
10	42.94022617124394	24.05823175209282	19.471288001174916	13.530254075488324
11	33.71420179174622	23.319136437068586	24.878102511381993	18.0885592598032
12	28.807460713761195	21.13397708914672	28.17888089293582	21.879681304156264
13	25.573138493170806	25.21313702452636	30.73156851226318	18.482155970039653
14	19.53352180936995	30.95002937288882	28.213026876193275	21.303421941547953
15	18.396423850785727	25.417829343515937	34.83716404758408	21.348582758114258
16	20.83106917315318	25.806652959318548	29.202158907328535	24.160118960199735
17	20.11822587751505	28.51538405052137	28.15354677632545	23.212843295638123
18	20.520450873843444	25.954618886767513	29.97705243060655	23.547877808782495
19	22.260978117197826	26.85177705977383	26.991114701130858	23.89613012189749
20	25.873109120281978	25.945256278454988	30.713026876193272	17.46860772506976
21	22.815574974298723	30.126303421941547	29.011602291085325	18.046519312674402
22	21.593846379791454	24.157365251872523	31.01354824496989	23.235240123366133
23	23.330518431487736	26.219525627845496	28.652151564106333	21.797804376560435
24	21.75301072110442	26.869033631957702	29.02280070494933	22.355154941988545
25	19.006645616096343	31.48241298281686	27.362681744749594	22.148259656337203
26	19.37747833749449	26.986158026141872	30.26215303275077	23.374210603612866
27	22.972169187839626	27.33330885592598	28.33547510647672	21.359046849757675
28	17.441254222352768	27.939859010133645	31.73244969892789	22.886437068585696
29	19.977970333382288	23.902371860772504	30.806469378763403	25.3131884270818
30	18.44507269789984	28.9868189161404	31.37171390806286	21.196394477896902
31	22.21104420619768	25.50007343222206	27.103649581436333	25.185232780143927
32	23.59469085034513	26.352805110882656	26.623219268615067	23.429284770157143
33	23.262593626083124	24.70865765898076	28.27140549273021	23.757343222205904
34	19.66111029519753	25.98362461448083	28.034219415479512	26.321045674842118
35	21.539506535467766	24.007930679982376	28.387428403583492	26.06513438096637
36	22.10089587310912	27.511565574974302	28.040093993244238	22.347444558672343
37	19.720039653399912	26.36198413864004	30.456197679541784	23.46177852841827
38	19.318365398736965	26.77632545160817	28.965156410632986	24.940152739021883
39	22.419408136290205	26.353723013658392	27.66522249963284	23.561646350418563
40	20.1797253634895	24.920142458510792	29.69048318402115	25.209648993978558
41	20.36936407695697	26.290754883242766	26.93659127625202	26.403289763548244
42	20.74790718167132	23.895579380232046	29.278161257159642	26.078352180937
43	23.604971361433396	23.23322073725951	28.17722866793949	24.9845792333676
44	21.002533411661037	23.924768688500514	30.14576296078719	24.926934939051257
45	20.003304449992658	24.33488764870025	28.315648406520783	27.34615949478631
46	22.83852254369217	27.5154207666324	27.182405639594652	22.463651050080777
47	19.148002643559995	25.24379497723601	33.218350712292555	22.38985166691144
48	21.028601850491995	24.378579820825376	29.19004259068879	25.40277573799383
49	20.40204141577324	23.238177412248497	32.495410486121315	23.864370685856954
50	21.24908209722426	23.602951975326775	30.00605815831987	25.141907769129094
51	20.384050521368778	22.972903510060213	27.704692318989572	28.938353649581437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	694.0
2	1130.0
3	3341.0
4	5552.0
5	3771.0
6	1990.0
7	1868.5
8	1747.0
9	1765.5
10	1784.0
11	1748.0
12	1712.0
13	1759.0
14	1806.0
15	1682.5
16	1559.0
17	1480.0
18	1401.0
19	1521.0
20	1641.0
21	2276.5
22	2912.0
23	2767.5
24	2623.0
25	3276.5
26	4758.0
27	5586.0
28	6877.5
29	8169.0
30	9053.0
31	9937.0
32	11154.5
33	12372.0
34	14130.0
35	15888.0
36	15339.5
37	14791.0
38	15854.0
39	16917.0
40	19672.0
41	22427.0
42	26361.5
43	30296.0
44	33576.5
45	36857.0
46	50149.0
47	63441.0
48	55999.5
49	48558.0
50	47135.5
51	45713.0
52	40321.5
53	34930.0
54	31515.0
55	28100.0
56	27187.5
57	26275.0
58	24104.5
59	21934.0
60	20991.5
61	20049.0
62	17850.0
63	15651.0
64	13830.5
65	12010.0
66	10010.0
67	8010.0
68	7261.5
69	6513.0
70	5625.5
71	4738.0
72	3594.5
73	2451.0
74	1897.0
75	1055.0
76	767.0
77	640.5
78	514.0
79	350.0
80	186.0
81	155.0
82	124.0
83	114.5
84	105.0
85	56.5
86	8.0
87	10.5
88	13.0
89	7.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.03078388917858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8326054513449	20.976165912708158
2	10.345805865067389	5.800020967262005
3	3.6082452846357684	3.034258313783187
4	1.8595371081795102	2.0849713125315175
5	1.0611572598480843	1.4872534911617286
6	0.70650519309808	1.18823366305888
7	0.569793785636493	1.1180236526601435
8	0.4287687714761998	0.9614979817342363
9	0.3375076644838178	0.8514543963678561
>10	5.401333685403245	38.70727472961711
>50	0.7630689988417038	13.083475813704021
>100	0.07039626969296833	3.3558495559836428
>500	0.010625852029127294	2.247285507285435
>1k	0.0026564630072818235	1.7159938540554291
>5k	0.0019923472554613676	3.388240848086664
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCG	7005	1.2859817888089293	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGC	5674	1.0416360699074754	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTC	5522	1.0137318255250403	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3216	0.5903950653546777	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	3164	0.5808488764870025	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCT	1428	0.26215303275077106	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTACGT	1410	0.2588485827581143	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTC	999	0.18339697459245116	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	995	0.18266265237186077	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTA	988	0.18137758848582758	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCT	982	0.180276105154942	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGCT	938	0.17219856072844764	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCC	908	0.1666911440740197	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	766	0.14062270524306067	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	720	0.13217799970627112	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	685	0.12575268027610514	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	652	0.11969452195623441	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	631	0.11583933029813483	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	612	0.11235129975033045	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTTCTGC	581	0.10666030254075488	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTACGTCGTATGCCGTCTT	551	0.10115288588632691	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1806432662652372	0.0
2	0.0	0.0	0.0	1.1741812307240418	0.0
3	0.0	0.0	0.0	1.4484505801145542	0.0
4	0.0	0.0	0.0	1.9821192539286239	0.0
5	0.0	0.0	0.0	3.9513878689969157	0.0
6	0.0	0.0	0.0	4.642568659127625	0.0
7	0.0	0.0	0.0	5.4679468350712295	0.0
8	0.0	0.0	0.0	6.4433103245704215	0.0
9	0.0	0.0	0.0	6.76457629607872	0.0
10	0.0	0.0	0.0	8.861433396974592	0.0
11	0.0	0.0	0.0	10.059480099867821	0.0
12	0.0	0.0	0.0	12.03517403436628	0.0
13	0.0	0.0	0.0	12.480540461154355	0.0
14	0.0	0.0	0.0	12.686334263474812	0.0
15	0.0	0.0	0.0	13.173006315171097	0.0
16	0.0	0.0	0.0	13.779556469378763	0.0
17	0.0	0.0	0.0	14.48156851226318	0.0
18	0.0	0.0	0.0	15.19349390512557	0.0
19	0.0	0.0	0.0	15.92653106182993	0.0
20	0.0	0.0	0.0	16.410449405199003	0.0
21	0.0	0.0	0.0	16.92117050961962	0.0
22	0.0	0.0	0.0	17.468791305624908	0.0
23	0.0	0.0	0.0	17.959134968424145	0.0
24	0.0	0.0	0.0	18.381920986929064	0.0
25	0.0	0.0	0.0	18.77074460273168	0.0
26	0.0	0.0	0.0	19.138640035247466	0.0
27	0.0	0.0	0.0	19.546923189895725	0.0
28	0.0	0.0	0.0	19.937399030694667	0.0
29	0.0	0.0	0.0	20.313004846526656	0.0
30	0.0	0.0	0.0	20.742950506682334	0.0
31	0.0	0.0	0.0	21.113416066970185	0.0
32	0.0	0.0	0.0	21.481311499485976	0.0
33	0.0	0.0	0.0	21.84994125422235	0.0
34	0.0	0.0	0.0	22.220773975620503	0.0
35	0.0	0.0	0.0	22.616573652518724	0.0
36	0.0	0.0	0.0	23.001725657218387	0.0
37	0.0	0.0	0.0	23.35107945366427	0.0
38	0.0	0.0	0.0	23.700616830665297	0.0
39	0.0	0.0	0.0	24.08338228814804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	30	2.1633914E-6	45.000004	2
TCTATTG	30	2.1633914E-6	45.000004	18
AGACTTA	35	1.2103555E-7	45.0	20
CGGGTAT	40	6.8030204E-9	45.0	6
AATTTCG	20	7.0300145E-4	45.0	39
ATTAGCG	20	7.0300145E-4	45.0	1
TTAATCG	35	1.2103555E-7	45.0	20
CGTTGAT	35	1.2103555E-7	45.0	25
ACCGAAT	20	7.0300145E-4	45.0	39
TACGGGT	25	3.888095E-5	45.0	4
CGACACG	20	7.0300145E-4	45.0	40
ACGGGTA	40	6.8030204E-9	45.0	5
TCGATAG	40	6.8030204E-9	45.0	1
CTGAACG	25	3.888095E-5	45.0	34
CCCCGTA	20	7.0300145E-4	45.0	40
TACGCCT	25	3.888095E-5	45.0	29
TACGATA	20	7.0300145E-4	45.0	1
TGTTCGT	20	7.0300145E-4	45.0	33
ACAGCGA	25	3.888095E-5	45.0	45
CGAATGT	20	7.0300145E-4	45.0	45
>>END_MODULE