FastQCFastQC Report
Sat 18 Jun 2016
SRR3551350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168062
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70930.6072451633560547No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC43880.3756649903857843No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG42910.36736063667853247No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC33780.28919697755769813No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC31860.2727594939309728No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC30230.25880475522703417No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC18940.1621489270261339No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT16230.13894810378216224No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC12410.10624436031649005No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12020.10290549645481148No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA207.033333E-445.00000414
TGTTACG406.8157533E-945.0000041
AGATTCG207.033333E-445.0000041
AAGTCGA207.033333E-445.00000427
CTAGACG453.8562575E-1045.01
CGCATCG351.2119563E-745.021
TAGTTCG253.8908453E-545.01
CCGGTCA253.8908453E-545.023
GCTCGCC253.8908453E-545.014
AGTCGAA253.8908453E-545.028
CGTAAGG1650.043.6363642
TACGGCT4750.041.2105267
CGTTTTT28600.041.1451031
GCGAATG556.184564E-1140.909091
TCGACGG556.184564E-1140.909092
CGATATT451.9283107E-840.010
TTACGAG403.4582808E-739.3750041
TGCAACG403.4582808E-739.3750041
AACGCGT356.2483778E-638.57142627
GATTACG356.2483778E-638.5714261