##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551349_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	865373
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6947489695195	33.0	31.0	34.0	30.0	34.0
2	31.825732949837814	33.0	31.0	34.0	30.0	34.0
3	31.78391514410549	33.0	31.0	34.0	30.0	34.0
4	35.47369978032594	37.0	35.0	37.0	33.0	37.0
5	33.31904392672293	37.0	35.0	37.0	30.0	37.0
6	34.29728799026547	37.0	35.0	37.0	30.0	37.0
7	35.4811162354268	37.0	35.0	37.0	32.0	37.0
8	35.45447107778958	37.0	35.0	37.0	33.0	37.0
9	37.392379933277326	39.0	37.0	39.0	34.0	39.0
10	36.8170615445594	39.0	37.0	39.0	32.0	39.0
11	36.87298540629301	39.0	37.0	39.0	33.0	39.0
12	36.94612149905301	39.0	37.0	39.0	33.0	39.0
13	37.07824256130016	39.0	37.0	39.0	33.0	39.0
14	38.14234671060918	40.0	38.0	41.0	33.0	41.0
15	38.24641975194511	40.0	38.0	41.0	34.0	41.0
16	38.312587751177816	40.0	38.0	41.0	34.0	41.0
17	38.16097567176235	40.0	37.0	41.0	34.0	41.0
18	38.15926427101377	40.0	37.0	41.0	34.0	41.0
19	38.06439766435976	40.0	37.0	41.0	34.0	41.0
20	38.21899805055161	40.0	37.0	41.0	34.0	41.0
21	38.13524572640931	40.0	37.0	41.0	34.0	41.0
22	38.27802346502607	40.0	37.0	41.0	34.0	41.0
23	38.32155729379123	40.0	37.0	41.0	34.0	41.0
24	38.2057736952736	40.0	37.0	41.0	34.0	41.0
25	37.8978093839304	40.0	37.0	41.0	33.0	41.0
26	38.01901029960491	40.0	37.0	41.0	34.0	41.0
27	37.631910170527625	40.0	37.0	41.0	33.0	41.0
28	37.7177771897205	40.0	37.0	41.0	33.0	41.0
29	37.96709973618312	40.0	37.0	41.0	33.0	41.0
30	37.717681277322036	40.0	36.0	41.0	33.0	41.0
31	37.696844019861956	40.0	36.0	41.0	33.0	41.0
32	37.80548503362134	40.0	37.0	41.0	33.0	41.0
33	37.80759972867192	40.0	37.0	41.0	33.0	41.0
34	37.73611956924933	40.0	37.0	41.0	33.0	41.0
35	37.72947850233368	40.0	37.0	41.0	33.0	41.0
36	37.55310715726051	40.0	37.0	41.0	33.0	41.0
37	37.55094739493837	40.0	37.0	41.0	33.0	41.0
38	37.419107136460234	40.0	36.0	41.0	33.0	41.0
39	37.35406350787464	40.0	36.0	41.0	33.0	41.0
40	37.24459510523208	40.0	36.0	41.0	32.0	41.0
41	37.114101087045704	40.0	36.0	41.0	32.0	41.0
42	37.314729024362904	40.0	36.0	41.0	33.0	41.0
43	37.25635650754068	40.0	36.0	41.0	33.0	41.0
44	37.32424168537729	40.0	36.0	41.0	33.0	41.0
45	37.308787077942114	40.0	36.0	41.0	33.0	41.0
46	37.186561170732155	40.0	36.0	41.0	32.0	41.0
47	37.1338266851404	40.0	35.0	41.0	32.0	41.0
48	37.1285283918033	40.0	35.0	41.0	32.0	41.0
49	37.11089899962213	39.0	35.0	41.0	32.0	41.0
50	37.0371065424967	39.0	35.0	41.0	32.0	41.0
51	36.161197541407	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	28.0
10	36.0
11	42.0
12	32.0
13	37.0
14	51.0
15	54.0
16	101.0
17	121.0
18	220.0
19	393.0
20	727.0
21	1179.0
22	1861.0
23	2531.0
24	3493.0
25	4596.0
26	5879.0
27	6864.0
28	7866.0
29	8816.0
30	10971.0
31	14702.0
32	19548.0
33	28018.0
34	47857.0
35	60178.0
36	68699.0
37	103022.0
38	185017.0
39	282343.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.78556529958758	20.956512394077468	24.170848870949293	13.087073435385665
2	30.45126205693961	26.736447751432042	26.623317344081688	16.188972847546665
3	32.07125713420687	24.773594738916053	27.583596899833946	15.571551227043138
4	29.054407752495166	24.89978309931093	28.080261343952262	17.965547804241638
5	26.23111652431957	34.10078659722455	23.037695883740305	16.630400994715576
6	25.945921585258613	35.41397755649876	23.83966220346602	14.80043865477661
7	80.14024010455607	5.585799418285525	10.227959504167567	4.0460009729908375
8	81.64456251812801	6.56456811109198	7.867936716306148	3.9229326544738514
9	74.41034097435441	8.822438416728971	9.992338563833169	6.77488204508345
10	35.99973652979698	37.249948865980336	15.961903133099831	10.788411471122856
11	26.069914360628303	25.06260306249444	31.264552973111016	17.60292960376624
12	25.492013270578123	22.127452555140962	32.750848478055126	19.629685696225792
13	23.15001739134454	23.880569419198427	34.22813052868532	18.741282660771713
14	18.500923879067177	28.804688845156946	31.28512213808381	21.40926513769207
15	17.551622248440847	28.681736083746546	34.604731139057954	19.161910528754653
16	20.86441338012626	27.31954891127872	31.906588257317942	19.909449451277077
17	19.791350088343407	28.253365889622163	30.334549379285004	21.62073464274943
18	19.950587781222666	27.83146689346675	31.11802656195652	21.09991876335407
19	21.668806399090336	28.232681167542783	29.74821262045384	20.350299812913043
20	24.264334570179564	27.517036006438843	30.807871287872395	17.410758135509198
21	23.076754185767292	27.418350237412074	30.76731074345976	18.737584833360874
22	21.157003974008894	25.226000811210884	31.620353304297684	21.99664191048253
23	20.899658297635817	27.54788975389803	31.35098968883938	20.201462259626773
24	21.42925651713192	26.185818138536792	31.178925157128777	21.206000187202513
25	19.898124854831384	30.207552119143998	28.76898169922103	21.125341326803586
26	19.41729173431572	30.124813230826476	30.07200363311543	20.38589140174237
27	21.391700457490582	30.033869787941153	29.285637522779197	19.288792231789067
28	18.211453327062436	29.246232549432445	31.76341300225452	20.778901121250605
29	20.592276394109824	27.717527586370274	30.76234178787644	20.927854231643465
30	21.537880197325315	28.274512840127898	29.84262277653682	20.344984186009963
31	20.707255715165598	28.326975766519176	28.135728755114847	22.83003976320038
32	22.796643759396236	29.897396845059877	27.396509944266807	19.909449451277077
33	21.72785608055717	28.911579168751512	27.694762836372295	21.665801914319026
34	19.67105514038455	28.629619828675036	29.4617465532204	22.237578477720014
35	21.06536718848404	28.19212062313014	28.27913512439145	22.46337706399437
36	20.59874759207879	29.476191191543993	27.70354517647304	22.22151603990418
37	21.455719094540733	28.805497744903064	29.286330865418726	20.452452295137473
38	20.32533947788988	28.431786062195147	27.551818695522044	23.691055764392928
39	21.91921864906809	26.712989658794534	29.25085483369599	22.116936858441388
40	21.34062421637837	27.42979039096436	30.873045495988432	20.356539896668835
41	18.67761069504133	27.867751824935606	30.312246857713376	23.142390622309687
42	20.597476463906318	27.973717691677464	29.160720290556792	22.268085553859436
43	22.065167274689642	27.339193619398806	27.595961510238936	22.99967759567262
44	20.958245750676298	27.43221709020272	28.760430473333464	22.849106685787515
45	20.850315413122434	27.021296019173235	28.01878496324706	24.10960360445727
46	21.22945827984002	28.572303503807028	27.877920850315412	22.320317366037536
47	19.492403853598393	27.690140552108744	31.00709173963135	21.810363854661517
48	20.453838980416535	25.679215783251845	30.139257869150065	23.727687367181552
49	19.50234176476502	25.834293420293907	31.26293517361877	23.400429641322297
50	19.250427272401613	26.74372784914713	30.10563075113275	23.90021412731851
51	18.988343754658395	25.617623845440058	28.82144462561231	26.572587774289236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	955.0
2	1585.0
3	3749.5
4	5914.0
5	4041.0
6	2168.0
7	2155.0
8	2142.0
9	2175.5
10	2209.0
11	2135.0
12	2061.0
13	2072.5
14	2084.0
15	2124.5
16	2165.0
17	2253.0
18	2341.0
19	2657.5
20	2974.0
21	3855.5
22	4737.0
23	5536.0
24	6335.0
25	7729.0
26	11124.5
27	13126.0
28	15514.5
29	17903.0
30	19290.5
31	20678.0
32	23905.5
33	27133.0
34	30196.5
35	33260.0
36	35251.0
37	37242.0
38	39921.0
39	42600.0
40	45165.5
41	47731.0
42	52974.0
43	58217.0
44	60828.5
45	63440.0
46	74454.0
47	85468.0
48	81895.5
49	78323.0
50	73886.0
51	69449.0
52	59998.0
53	50547.0
54	44623.0
55	38699.0
56	35043.5
57	31388.0
58	29731.0
59	28074.0
60	26509.0
61	24944.0
62	23124.5
63	21305.0
64	17112.0
65	12919.0
66	9737.0
67	6555.0
68	5281.0
69	4007.0
70	3384.0
71	2761.0
72	2120.5
73	1480.0
74	1284.0
75	792.0
76	496.0
77	342.0
78	188.0
79	165.5
80	143.0
81	89.0
82	35.0
83	20.5
84	6.0
85	4.5
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	865373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.4905404683063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2908976458198	20.148063876909045
2	10.696871213954568	5.881255419829577
3	3.9246041853645943	3.23668470539549
4	1.9091320260423834	2.0993228488503095
5	1.097051167507987	1.5079264758090496
6	0.7418378379256421	1.2236113862649427
7	0.5492857374659376	1.057011325612958
8	0.3894925919963774	0.8565889489905544
9	0.3576844516044043	0.8849645002543342
>10	6.1665339671246615	43.77081431584904
>50	0.8159175599950586	13.695351038900752
>100	0.05299831186356324	2.3980963837039777
>500	0.0038466516675166873	0.7778245007408295
>1k	0.0038466516675166873	2.46248427288917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG	3657	0.4225923387949474	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	3298	0.3811073375295971	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	3292	0.3804139948900648	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3254	0.3760228248396934	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2814	0.3251776979406568	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCC	1302	0.15045535277851285	No Hit
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	1126	0.1301173020188982	TruSeq Adapter, Index 20 (95% over 22bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1117	0.12907728805959973	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	1098	0.12688170303441407	TruSeq Adapter, Index 23 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCT	935	0.10804589466045277	No Hit
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	907	0.10481029567596863	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1555710658871955E-4	0.0	0.0	0.13554848602856803	0.0
2	1.1555710658871955E-4	0.0	0.0	0.7834771826715186	0.0
3	1.1555710658871955E-4	0.0	0.0	1.0123958108237718	0.0
4	1.1555710658871955E-4	0.0	0.0	1.4386859770295584	0.0
5	1.1555710658871955E-4	0.0	0.0	2.680000416005584	0.0
6	1.1555710658871955E-4	0.0	0.0	3.2774306570692637	0.0
7	1.1555710658871955E-4	0.0	0.0	3.8918477928014856	0.0
8	1.1555710658871955E-4	0.0	0.0	4.729636815569703	0.0
9	1.1555710658871955E-4	0.0	0.0	5.038752075694527	0.0
10	1.1555710658871955E-4	0.0	0.0	6.20807443726578	0.0
11	1.1555710658871955E-4	0.0	0.0	7.370001144015355	0.0
12	1.1555710658871955E-4	0.0	0.0	8.694863371055025	0.0
13	1.1555710658871955E-4	0.0	0.0	9.090299789801623	0.0
14	1.1555710658871955E-4	0.0	0.0	9.25577756643667	0.0
15	1.1555710658871955E-4	0.0	0.0	9.603488900162127	0.0
16	1.1555710658871955E-4	0.0	0.0	10.171914307472038	0.0
17	1.1555710658871955E-4	0.0	0.0	10.918875444461522	0.0
18	1.1555710658871955E-4	0.0	0.0	11.678663420282351	0.0
19	2.311142131774391E-4	0.0	0.0	12.282449302208413	0.0
20	2.311142131774391E-4	0.0	0.0	12.777842618154253	0.0
21	2.311142131774391E-4	0.0	0.0	13.342223526733559	0.0
22	2.311142131774391E-4	0.0	0.0	14.016845914998504	0.0
23	2.311142131774391E-4	0.0	0.0	14.591742520277384	0.0
24	3.4667131976615865E-4	0.0	0.0	15.067028899676787	0.0
25	3.4667131976615865E-4	0.0	0.0	15.481416683903936	0.0
26	3.4667131976615865E-4	0.0	0.0	15.862292907220354	0.0
27	3.4667131976615865E-4	0.0	0.0	16.258538225713075	0.0
28	3.4667131976615865E-4	0.0	0.0	16.661485856387937	0.0
29	3.4667131976615865E-4	0.0	0.0	17.10453180304909	0.0
30	4.622284263548782E-4	0.0	0.0	17.586636051737226	0.0
31	4.622284263548782E-4	0.0	0.0	18.039966580884773	0.0
32	4.622284263548782E-4	0.0	0.0	18.474345744551773	0.0
33	5.777855329435977E-4	0.0	0.0	18.884920144261493	0.0
34	5.777855329435977E-4	0.0	0.0	19.315023694984706	0.0
35	5.777855329435977E-4	0.0	0.0	19.73969606169825	0.0
36	5.777855329435977E-4	0.0	0.0	20.180777537547392	0.0
37	5.777855329435977E-4	0.0	0.0	20.610996645377195	0.0
38	5.777855329435977E-4	0.0	0.0	21.0115175768137	0.0
39	5.777855329435977E-4	0.0	0.0	21.428794288705564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCA	20	7.0323196E-4	45.0	35
ACCGCAC	25	3.8900038E-5	45.0	35
TAGAACG	20	7.0323196E-4	45.0	1
CGCGCAT	20	7.0323196E-4	45.0	42
ATCGTTG	45	3.8380676E-10	45.0	23
TATTCGC	25	3.8900038E-5	45.0	20
TGTTCGC	20	7.0323196E-4	45.0	28
TCGTCTC	20	7.0323196E-4	45.0	14
CTCGCGC	25	3.8900038E-5	45.0	33
TATCGTG	20	7.0323196E-4	45.0	25
GTGCGAT	20	7.0323196E-4	45.0	16
TCGCGCA	25	3.8900038E-5	45.0	34
ATGGGCG	160	0.0	42.1875	5
CGAATAT	150	0.0	42.000004	14
GCTACGA	145	0.0	41.89655	10
CGTAAGG	105	0.0	40.714287	2
AAGTACG	45	1.9274012E-8	40.0	1
CGACAGG	45	1.9274012E-8	40.0	2
CGTTTTT	1815	0.0	39.545456	1
TTAGCGG	205	0.0	39.512196	2
>>END_MODULE