FastQCFastQC Report
Sat 18 Jun 2016
SRR3551347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088728
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC150641.3836330102651901No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG142871.3122653224680545No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC103780.9532224761372905No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.739027562439838No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC58440.5367731885282642No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT42390.3893534473256865No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT32490.29842164434091895No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC31700.2911654701633466No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCCCTT29050.26682513906136335No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT24070.2210836866508439No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC21560.1980292598334938No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTA19680.18076140229699245No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC16910.1553188675224666No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT13240.12160980520387094No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC11170.10259679185251046No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA207.033121E-445.00000438
AGCCGGT207.033121E-445.00000413
ACGATAG207.033121E-445.0000041
CGTAATC207.033121E-445.00000443
CTCGACG207.033121E-445.0000041
ACGGTCG207.033121E-445.00000429
ACTCGTC253.89067E-545.042
GCATCGC253.89067E-545.022
AATCGCG551.8189894E-1245.01
AGTAACG253.89067E-545.01
AACGTCC302.165396E-644.99999640
ATAGGCG302.165396E-644.9999961
CGCCCCA302.165396E-644.99999614
CCGATGA20500.042.36585218
CGTTTTT38300.042.0039141
ACGGGTC750.042.05
TATAGCG700.041.7857131
CGATGAA21500.041.75581419
TTGTGCG1150.041.0869561
CGGCGAT4550.041.04395731
Produced by FastQC (version 0.11.5)