##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1088728
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.612422937593227	31.0	31.0	34.0	30.0	34.0
2	31.755123410071203	31.0	31.0	34.0	30.0	34.0
3	31.71730220955096	31.0	31.0	34.0	30.0	34.0
4	35.43855214525575	37.0	35.0	37.0	33.0	37.0
5	33.43640192959123	37.0	35.0	37.0	30.0	37.0
6	34.320224151486876	37.0	35.0	37.0	30.0	37.0
7	35.4040871549184	37.0	35.0	37.0	32.0	37.0
8	35.29938699105745	37.0	35.0	37.0	33.0	37.0
9	37.06854696489849	39.0	37.0	39.0	33.0	39.0
10	36.58687018245145	38.0	35.0	39.0	32.0	39.0
11	36.77046608519299	39.0	37.0	39.0	33.0	39.0
12	36.83956598893388	39.0	37.0	39.0	33.0	39.0
13	36.91625640196633	39.0	37.0	39.0	33.0	39.0
14	38.00122344607652	40.0	37.0	41.0	33.0	41.0
15	38.014514185361264	40.0	37.0	41.0	33.0	41.0
16	38.13744755347525	40.0	37.0	41.0	34.0	41.0
17	37.96361533826631	40.0	37.0	41.0	33.0	41.0
18	37.92247742319477	39.0	37.0	41.0	33.0	41.0
19	37.81912011080821	39.0	37.0	41.0	33.0	41.0
20	37.80129012939871	40.0	36.0	41.0	33.0	41.0
21	37.83333945668707	39.0	36.0	41.0	33.0	41.0
22	37.958221888295334	40.0	37.0	41.0	33.0	41.0
23	37.936434995701404	40.0	37.0	41.0	33.0	41.0
24	37.85121260774041	40.0	36.0	41.0	33.0	41.0
25	37.6830999110889	39.0	36.0	41.0	33.0	41.0
26	37.73230963105569	40.0	36.0	41.0	33.0	41.0
27	37.30372875502421	40.0	36.0	41.0	33.0	41.0
28	37.352424113277145	40.0	36.0	41.0	32.0	41.0
29	37.64297602339611	40.0	36.0	41.0	33.0	41.0
30	37.3672707967463	40.0	36.0	41.0	32.0	41.0
31	37.335762467760546	39.0	36.0	41.0	32.0	41.0
32	37.26070974568487	40.0	36.0	41.0	32.0	41.0
33	37.19708228317817	40.0	36.0	41.0	32.0	41.0
34	37.08687844897899	40.0	36.0	41.0	31.0	41.0
35	36.9923938761564	40.0	36.0	41.0	31.0	41.0
36	36.776076301886235	40.0	36.0	41.0	31.0	41.0
37	36.75529608864657	40.0	36.0	41.0	31.0	41.0
38	36.675742701574684	40.0	35.0	41.0	30.0	41.0
39	36.540293810758975	39.0	35.0	41.0	30.0	41.0
40	36.59536725426369	39.0	35.0	41.0	30.0	41.0
41	36.477529741129096	39.0	35.0	41.0	30.0	41.0
42	36.61337083275161	39.0	35.0	41.0	30.0	41.0
43	36.443087713368264	39.0	35.0	41.0	30.0	41.0
44	36.62748546928158	39.0	35.0	41.0	30.0	41.0
45	36.62984877765613	39.0	35.0	41.0	31.0	41.0
46	36.4712728982813	39.0	35.0	41.0	30.0	41.0
47	36.35813077279173	39.0	35.0	41.0	30.0	41.0
48	36.38464244512863	39.0	35.0	41.0	30.0	41.0
49	36.39748219941069	39.0	35.0	41.0	30.0	41.0
50	36.29604180291129	39.0	35.0	41.0	30.0	41.0
51	35.48352297359855	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	37.0
10	55.0
11	37.0
12	34.0
13	32.0
14	55.0
15	63.0
16	85.0
17	152.0
18	259.0
19	627.0
20	1411.0
21	2939.0
22	5272.0
23	6954.0
24	8442.0
25	10014.0
26	11354.0
27	11809.0
28	11569.0
29	12485.0
30	15113.0
31	19946.0
32	26634.0
33	37481.0
34	60666.0
35	75863.0
36	88062.0
37	129857.0
38	226761.0
39	324576.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41295530196707	19.65642474520725	22.13822001454909	14.792399938276594
2	29.585626529307596	30.103386704484496	23.83037820282017	16.480608563387733
3	28.22054728086354	28.758238972452254	28.393409556840645	14.62780418984356
4	25.827387556855342	22.95366703161855	33.847021478275565	17.371923933250546
5	27.520464248186876	30.33512502663659	24.60531923492369	17.539091490252844
6	23.428533113872334	36.65598753775048	25.640012932523092	14.2754664158541
7	74.11538970247848	4.860718195913029	16.515603529990962	4.508288571617521
8	74.67181885650042	9.52423378474697	10.716634457826013	5.0873129009265865
9	69.11193613097119	6.781951047460891	12.253198227656496	11.852914593911427
10	36.38190622451154	30.85701846558553	19.513964920531116	13.247110389371816
11	27.002244821479742	22.468146313863517	31.046873048180995	19.482735816475742
12	23.636574057064756	19.470611576077772	33.835723890632	23.05709047622547
13	23.836807724243336	22.37133609129186	35.710756038239126	18.081100146225687
14	18.482302283031206	28.863407572874035	31.114199322512142	21.540090821582616
15	16.293785040891756	26.09852966030083	36.82894166403363	20.778743634773793
16	18.701457113255103	25.62834794365535	31.17573902756244	24.494455915527112
17	18.934848740916006	28.281352183465476	30.91975222461441	21.864046851004108
18	18.63018127576401	26.43993724787091	31.859472705763054	23.070408770602025
19	19.545653276116717	27.585494264866888	29.388699473146644	23.480152985869747
20	22.876696475152656	27.136529968917856	32.58049760821803	17.406275947711457
21	21.218706600730393	30.065636228699915	30.88585946168373	17.829797708885966
22	19.495870410240208	23.76176602420439	32.37916173736691	24.36320182818849
23	20.141945462962283	27.039536045734103	31.648676253389276	21.169842237914338
24	20.45717571330948	26.494037078131544	30.372599951503037	22.67618725705594
25	17.76458399159386	32.484054786870544	28.010944882468348	21.74041633906724
26	17.705891646030963	26.749932030773525	32.86183509563454	22.682341227560972
27	21.693848233902315	27.725382280973758	30.032753819135728	20.5480156659882
28	17.701850232564976	27.479866412914888	33.02312423304994	21.795159121470192
29	19.50367768625405	22.869532151281128	35.40700707614758	22.219783086317243
30	19.147390349104644	27.410886833074926	32.668214650491215	20.77350816732921
31	21.852749263360547	24.775150450801302	28.05971739497836	25.31238289085979
32	22.458410181422725	25.588668611443815	30.34697371611642	21.60594749101704
33	21.01929958630622	24.348138377997074	28.795254645788482	25.837307389908222
34	19.00676753054941	22.98710054301901	31.455606910082224	26.55052501634935
35	19.84958593882035	22.203984833677463	31.535608526647614	26.410820700854575
36	20.413271267019862	24.951870439632305	32.15816990102211	22.476688392325723
37	18.77126334584947	23.711156505573477	35.00837674791132	22.50920340066573
38	19.993607218699253	24.53597225385955	28.60650226686555	26.86391826057564
39	20.148191283773357	23.89917408204804	30.949970975303287	25.002663658875314
40	19.09981189057322	22.739839519145278	30.365251927019422	27.795096663262083
41	17.70910640674255	23.847646060356674	30.748726954758215	27.694520578142566
42	19.40723486490657	23.403733531240125	32.67675672895342	24.512274874899884
43	21.170485190056652	23.68286661131155	31.706541946197763	23.440106252434035
44	20.261259010515023	23.52800699531931	31.785900610620832	24.424833383544833
45	20.219926372794674	22.294181834213873	30.396848432298977	27.08904336069248
46	22.796327457363088	24.760546252140113	29.517014350691817	22.926111939804983
47	17.213574005628587	23.80236385947638	35.38101343953678	23.603048695358254
48	19.397957983996005	23.040924822361507	31.187404016430182	26.373713177212306
49	19.27083716042942	21.689439419212146	34.419983687385646	24.61973973297279
50	19.612703999529728	21.45568039032706	32.47404310351162	26.457572506631593
51	18.92134674592736	20.96189314502796	30.167038966573838	29.949721142470846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	407.0
1	955.0
2	1503.0
3	7266.5
4	13030.0
5	8583.5
6	4137.0
7	4058.0
8	3979.0
9	3908.5
10	3838.0
11	3779.0
12	3720.0
13	3625.5
14	3531.0
15	3522.0
16	3513.0
17	3504.0
18	3495.0
19	3440.5
20	3386.0
21	3799.5
22	4213.0
23	4605.0
24	4997.0
25	6203.0
26	9040.5
27	10672.0
28	12618.0
29	14564.0
30	17308.5
31	20053.0
32	22569.0
33	25085.0
34	27667.5
35	30250.0
36	34746.5
37	39243.0
38	41855.5
39	44468.0
40	49145.5
41	53823.0
42	58627.5
43	63432.0
44	70271.0
45	77110.0
46	111595.5
47	146081.0
48	128673.5
49	111266.0
50	107115.5
51	102965.0
52	86769.0
53	70573.0
54	62509.5
55	54446.0
56	47855.0
57	41264.0
58	37232.5
59	33201.0
60	31049.5
61	28898.0
62	25321.5
63	21745.0
64	17925.0
65	14105.0
66	11815.5
67	9526.0
68	7558.0
69	5590.0
70	4627.5
71	3665.0
72	3085.5
73	2506.0
74	1989.0
75	1145.5
76	819.0
77	637.5
78	456.0
79	321.0
80	186.0
81	121.5
82	57.0
83	42.5
84	28.0
85	19.5
86	11.0
87	10.5
88	10.0
89	5.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1088728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.655124707184576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.09564903347949	22.714113613810945
2	10.8018158581649	6.62262024392178
3	4.040739986584532	3.7160816459416846
4	2.047386649887622	2.510515723045196
5	1.2329462880801092	1.8898061109178033
6	0.7909179200556646	1.4547412483472066
7	0.631705114942321	1.3555499353706253
8	0.4501121271415214	1.1038594711791565
9	0.3791614187658435	1.0460916518777972
>10	4.912990259957181	36.02479597665278
>50	0.5620380917166192	10.763276650836652
>100	0.04453875516653755	2.47219618347727
>500	0.005150740393376116	1.0031077424749413
>1k	0.0033328320192433695	2.343092501797717
>5k	6.05969458044249E-4	1.2901080132386051
>10k+	9.089541870663736E-4	3.690043287109902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	15064	1.3836330102651901	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG	14287	1.3122653224680545	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC	10378	0.9532224761372905	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8046	0.739027562439838	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	5844	0.5367731885282642	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT	4239	0.3893534473256865	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	3249	0.29842164434091895	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	3170	0.2911654701633466	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCCCTT	2905	0.26682513906136335	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	2407	0.2210836866508439	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC	2156	0.1980292598334938	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTA	1968	0.18076140229699245	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC	1691	0.1553188675224666	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT	1324	0.12160980520387094	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	1117	0.10259679185251046	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22705395654378321	0.0
2	0.0	0.0	0.0	1.2814954699429058	0.0
3	0.0	0.0	0.0	1.6105951165029282	0.0
4	0.0	0.0	0.0	2.3564196015901127	0.0
5	0.0	0.0	0.0	4.806342814734259	0.0
6	0.0	0.0	0.0	5.792723251353873	0.0
7	0.0	0.0	0.0	6.9254212255035235	0.0
8	0.0	0.0	0.0	8.311534194031935	0.0
9	0.0	0.0	0.0	8.861901227854892	0.0
10	0.0	0.0	0.0	11.411573873364146	0.0
11	0.0	0.0	0.0	12.982673358267629	0.0
12	0.0	0.0	0.0	15.296749968770897	0.0
13	0.0	0.0	0.0	15.89423620959505	0.0
14	0.0	0.0	0.0	16.158856941311328	0.0
15	0.0	0.0	0.0	16.69434422555496	0.0
16	0.0	0.0	0.0	17.410409211483493	0.0
17	0.0	0.0	0.0	18.322850151736706	0.0
18	0.0	0.0	0.0	19.293524186022587	0.0
19	0.0	0.0	0.0	20.212210947086877	0.0
20	0.0	0.0	0.0	20.8323842135042	0.0
21	0.0	0.0	0.0	21.479377769286728	0.0
22	0.0	0.0	0.0	22.259646119140868	0.0
23	9.185030604521975E-5	0.0	0.0	22.929326700516565	0.0
24	9.185030604521975E-5	0.0	0.0	23.4638036313937	0.0
25	9.185030604521975E-5	0.0	0.0	23.941884474359068	0.0
26	9.185030604521975E-5	0.0	0.0	24.37440756552601	0.0
27	9.185030604521975E-5	0.0	0.0	24.876185787451043	0.0
28	9.185030604521975E-5	0.0	0.0	25.345724551954206	0.0
29	9.185030604521975E-5	0.0	0.0	25.841348803374213	0.0
30	9.185030604521975E-5	0.0	0.0	26.371049518336996	0.0
31	9.185030604521975E-5	0.0	0.0	26.870439632304855	0.0
32	9.185030604521975E-5	0.0	0.0	27.339151744053613	0.0
33	9.185030604521975E-5	0.0	0.0	27.797944022749483	0.0
34	9.185030604521975E-5	0.0	0.0	28.2289975090197	0.0
35	9.185030604521975E-5	0.0	0.0	28.701659183928403	0.0
36	1.837006120904395E-4	0.0	0.0	29.16072701354241	0.0
37	1.837006120904395E-4	0.0	0.0	29.604088440822686	0.0
38	1.837006120904395E-4	0.0	0.0	30.043959556473244	0.0
39	1.837006120904395E-4	0.0	0.0	30.481626264778715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.033121E-4	45.000004	38
AGCCGGT	20	7.033121E-4	45.000004	13
ACGATAG	20	7.033121E-4	45.000004	1
CGTAATC	20	7.033121E-4	45.000004	43
CTCGACG	20	7.033121E-4	45.000004	1
ACGGTCG	20	7.033121E-4	45.000004	29
ACTCGTC	25	3.89067E-5	45.0	42
GCATCGC	25	3.89067E-5	45.0	22
AATCGCG	55	1.8189894E-12	45.0	1
AGTAACG	25	3.89067E-5	45.0	1
AACGTCC	30	2.165396E-6	44.999996	40
ATAGGCG	30	2.165396E-6	44.999996	1
CGCCCCA	30	2.165396E-6	44.999996	14
CCGATGA	2050	0.0	42.365852	18
CGTTTTT	3830	0.0	42.003914	1
ACGGGTC	75	0.0	42.0	5
TATAGCG	70	0.0	41.785713	1
CGATGAA	2150	0.0	41.755814	19
TTGTGCG	115	0.0	41.086956	1
CGGCGAT	455	0.0	41.043957	31
>>END_MODULE