##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556101
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.547058897574363	31.0	31.0	34.0	30.0	34.0
2	31.688031490682448	31.0	31.0	34.0	30.0	34.0
3	31.639311923553453	31.0	31.0	34.0	30.0	34.0
4	35.36548756430936	37.0	35.0	37.0	33.0	37.0
5	33.404960609673424	37.0	35.0	37.0	30.0	37.0
6	34.264041963600135	37.0	35.0	37.0	30.0	37.0
7	35.301607082166726	37.0	35.0	37.0	32.0	37.0
8	35.171788937621045	37.0	35.0	37.0	32.0	37.0
9	36.95767135826046	39.0	37.0	39.0	33.0	39.0
10	36.54973826696949	38.0	35.0	39.0	32.0	39.0
11	36.659801007370966	39.0	35.0	39.0	32.0	39.0
12	36.55356490997139	39.0	35.0	39.0	32.0	39.0
13	36.56067512915819	39.0	35.0	39.0	32.0	39.0
14	37.51402532993107	39.0	36.0	41.0	33.0	41.0
15	37.63464730327764	39.0	36.0	41.0	33.0	41.0
16	37.721816720343966	39.0	36.0	41.0	33.0	41.0
17	37.54069134923332	39.0	36.0	41.0	33.0	41.0
18	37.502137201695376	39.0	36.0	41.0	33.0	41.0
19	37.38884303390931	39.0	36.0	41.0	32.0	41.0
20	37.44780894118155	39.0	35.0	41.0	33.0	41.0
21	37.33844751223249	39.0	35.0	41.0	33.0	41.0
22	37.48123812041338	39.0	35.0	41.0	33.0	41.0
23	37.50648713093485	39.0	35.0	41.0	33.0	41.0
24	37.415480281459665	39.0	35.0	41.0	33.0	41.0
25	37.14498805073179	39.0	35.0	41.0	32.0	41.0
26	37.19207302270631	39.0	35.0	41.0	33.0	41.0
27	36.78040140190361	39.0	35.0	41.0	32.0	41.0
28	36.80692895714987	39.0	35.0	41.0	31.0	41.0
29	36.989550459359	39.0	35.0	41.0	32.0	41.0
30	36.70541861999889	39.0	35.0	41.0	31.0	41.0
31	36.68503383378199	39.0	35.0	41.0	31.0	41.0
32	36.65978482326052	39.0	35.0	41.0	31.0	41.0
33	36.591050906220275	39.0	35.0	41.0	31.0	41.0
34	36.43428801602587	39.0	35.0	41.0	30.0	41.0
35	36.35678590759592	39.0	35.0	41.0	30.0	41.0
36	36.175513081256824	39.0	35.0	41.0	30.0	41.0
37	36.20451141069698	39.0	35.0	41.0	30.0	41.0
38	35.986754204721805	39.0	35.0	41.0	29.0	41.0
39	35.91617529909135	39.0	35.0	41.0	29.0	41.0
40	35.81181476026837	38.0	35.0	40.0	29.0	41.0
41	35.64509144921516	38.0	35.0	40.0	28.0	41.0
42	35.77487722553997	38.0	35.0	40.0	29.0	41.0
43	35.711946211209835	38.0	35.0	40.0	29.0	41.0
44	35.80794855610761	38.0	35.0	40.0	29.0	41.0
45	35.78475492761207	38.0	35.0	40.0	29.0	41.0
46	35.66892704742484	38.0	35.0	40.0	28.0	41.0
47	35.57529117912034	38.0	35.0	40.0	28.0	41.0
48	35.54440470346214	38.0	35.0	40.0	28.0	41.0
49	35.52808572543477	38.0	35.0	40.0	28.0	41.0
50	35.43680914078558	38.0	35.0	40.0	28.0	41.0
51	34.56026872816269	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	36.0
10	46.0
11	34.0
12	29.0
13	30.0
14	37.0
15	46.0
16	85.0
17	139.0
18	246.0
19	448.0
20	863.0
21	1513.0
22	2393.0
23	3394.0
24	4458.0
25	5588.0
26	6692.0
27	7539.0
28	7761.0
29	8435.0
30	10155.0
31	12571.0
32	16698.0
33	23903.0
34	43294.0
35	49598.0
36	46368.0
37	65879.0
38	105126.0
39	132645.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.557918435679845	19.784715366453216	22.972265829408688	15.685100368458249
2	32.67266198046757	27.543198088117087	22.61243910728447	17.171700824130866
3	30.586889791602605	26.568015522360145	27.106406929676442	15.738687756360806
4	27.536005150143588	23.303860269986927	31.481151805157698	17.678982774711788
5	26.430450583617006	30.837024209630986	24.67573336498226	18.05679184176975
6	24.84656564185283	34.92638927101372	26.10281225892419	14.124232828209266
7	73.47550175237951	6.06040988957042	15.72160452867375	4.742483829376319
8	73.08150857488118	9.100864770967863	12.533514595370265	5.284112058780689
9	67.11586564311159	7.804517524694256	14.627558662904761	10.45205816928939
10	41.27199915123332	24.721768168012645	20.72249465474797	13.283738026006068
11	33.774260431108736	22.538891316505456	26.241276314914018	17.44557193747179
12	29.835227773372104	19.544651061587732	30.17761162091059	20.44250954412957
13	26.79890883130942	22.193989940676246	31.798720016687614	19.20838121132672
14	19.054811985592547	28.411745348416922	30.88503707060408	21.64840559538645
15	18.79586621854663	23.949606276557674	36.68254507724316	20.57198242765253
16	21.65182224092386	23.920115230866333	32.15351168223039	22.27455084597942
17	21.58870421020642	25.326154781235783	30.08967795418458	22.995463054373218
18	22.768525861309367	23.85304108426347	30.52035511534775	22.858077939079415
19	23.275268341542272	25.822287677957785	28.535463881561085	22.366980098938864
20	26.673571887121224	25.38118075673304	29.868135464600854	18.07711189154488
21	23.733997960802082	27.968480545800134	29.43637936274166	18.861142130656123
22	21.703971041231718	23.14849280975938	31.737759867362225	23.40977628164668
23	22.66386861379498	25.345395890314887	30.963260271065867	21.02747522482427
24	23.341263547449113	23.589240084085443	30.13751099170834	22.9319853767571
25	20.975686071415083	27.085007939205287	28.760962487030234	23.178343502349392
26	20.097428344851025	25.89961176117288	31.09201386079147	22.910946033184622
27	21.9176012990446	26.636887903456387	28.889356429857166	22.55615436764185
28	19.228341614203178	26.89996960983706	31.095430506328885	22.776258269630876
29	20.922098683512527	25.436386555679636	30.51837705740504	23.1231377034028
30	21.181404097457115	27.008762796686213	29.262669910681694	22.547163195174978
31	24.28857347855875	25.50400017263051	25.800169393689277	24.40725695512146
32	25.115581522061643	27.285511085216534	26.242175432160703	21.35673196056112
33	23.296667332013428	26.099035966488103	26.393227129604153	24.211069571894313
34	20.981260598344544	26.848540103326552	27.749635407956468	24.42056389037243
35	20.273835148651052	28.317517860964102	26.70252346246455	24.706123527920287
36	21.25351330064143	30.083743780356446	27.312844249515827	21.349898669486297
37	21.970109746251133	27.257998097467905	27.567294430328303	23.20459772595266
38	21.745510258028666	28.552906756146818	25.121875342788453	24.579707643036066
39	22.277607844618156	25.808081625460122	26.90230731467845	25.012003215243272
40	22.149034078341884	25.888103060415286	27.870117119012555	24.092745742230278
41	18.179431434217886	27.456343362087104	28.28011458350192	26.084110620193098
42	21.022799815141493	26.390349954414756	28.9125536548217	23.67429657562205
43	22.552917545553775	24.217183569171787	28.160172342793842	25.06972654248059
44	21.81366334532756	24.603264514899273	27.843862895409288	25.73920924436388
45	21.470200557093047	24.684544713999795	26.683102529936107	27.16215219897105
46	21.88037784503175	27.53906214878232	26.568195345809485	24.01236466037644
47	19.97263087101084	26.015418062546193	30.63580176982239	23.37614929662058
48	20.64984598121564	24.379743967372832	30.051015912577032	24.91939413883449
49	21.51839324151548	23.259443878000578	31.207100868367437	24.015062012116502
50	19.53224324358345	24.299362885518995	30.715463557878874	25.45293031301868
51	19.2409292556568	24.12061837687758	28.634366778696673	28.004085588768945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	281.0
1	884.0
2	1487.0
3	4824.5
4	8162.0
5	5292.0
6	2422.0
7	2383.5
8	2345.0
9	2271.0
10	2197.0
11	2210.5
12	2224.0
13	2087.0
14	1950.0
15	2069.0
16	2188.0
17	2216.5
18	2245.0
19	2189.0
20	2133.0
21	2652.5
22	3172.0
23	3043.0
24	2914.0
25	3208.0
26	4436.0
27	5370.0
28	5990.5
29	6611.0
30	7994.0
31	9377.0
32	10262.5
33	11148.0
34	11690.0
35	12232.0
36	13031.5
37	13831.0
38	15175.0
39	16519.0
40	19609.0
41	22699.0
42	24712.5
43	26726.0
44	30209.5
45	33693.0
46	45788.5
47	57884.0
48	53185.5
49	48487.0
50	47260.5
51	46034.0
52	42101.0
53	38168.0
54	34953.0
55	31738.0
56	29947.5
57	28157.0
58	26777.5
59	25398.0
60	25116.0
61	24834.0
62	22777.0
63	20720.0
64	17369.0
65	14018.0
66	11750.5
67	9483.0
68	7537.5
69	5592.0
70	4959.0
71	4326.0
72	3491.5
73	2657.0
74	1955.5
75	1053.0
76	852.0
77	636.0
78	420.0
79	363.0
80	306.0
81	251.0
82	196.0
83	156.5
84	117.0
85	66.5
86	16.0
87	12.0
88	8.0
89	6.0
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.875015408078955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08449817324774	24.25194551588227
2	9.597131050582595	6.118174002213464
3	3.419436200083704	3.2698374469383307
4	1.651274271021528	2.1053757132710222
5	1.0151451209785958	1.6178883186314459
6	0.6858815548073954	1.3117491076561727
7	0.548988911201891	1.2249321002397335
8	0.4562199007454751	1.1633613092587416
9	0.36755579315354564	1.0544261913088153
>10	5.770321629851357	43.58539722832958
>50	0.34768913917931027	6.756932011483567
>100	0.04673854002082528	2.4721457847397716
>500	0.005129839758383262	1.2819475753012293
>1k	0.0039898753676314265	3.785887694745892
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4708	0.8466087994806699	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC	4457	0.8014731136969723	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG	3916	0.704188627605417	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC	3438	0.6182330188221205	No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	1755	0.31559015358720804	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTT	1447	0.2602045311912764	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCC	1117	0.20086279290992107	No Hit
GAACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCT	969	0.17424892240797984	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCT	962	0.17299015826261777	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGCACATT	871	0.15662622437291066	No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	849	0.152670108487487	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTC	736	0.1323500587123562	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	734	0.13199041181368132	No Hit
TCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	698	0.12551676763753347	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGCACATTCGTA	695	0.12497729728952116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.798234493374405E-4	0.0	0.0	0.13774476219247941	0.0
2	1.798234493374405E-4	0.0	0.0	0.8246703386615022	0.0
3	1.798234493374405E-4	0.0	0.0	1.1204799128215917	0.0
4	1.798234493374405E-4	0.0	0.0	1.6791913699130194	0.0
5	1.798234493374405E-4	0.0	0.0	3.4479348175960842	0.0
6	1.798234493374405E-4	0.0	0.0	4.414485857784827	0.0
7	1.798234493374405E-4	0.0	0.0	5.209665150754989	0.0
8	1.798234493374405E-4	0.0	0.0	6.198694122110911	0.0
9	1.798234493374405E-4	0.0	0.0	6.586033831983758	0.0
10	1.798234493374405E-4	0.0	0.0	8.158769719889014	0.0
11	1.798234493374405E-4	0.0	0.0	9.552041805355502	0.0
12	1.798234493374405E-4	0.0	0.0	11.248316402955577	0.0
13	1.798234493374405E-4	0.0	0.0	11.787247280619887	0.0
14	1.798234493374405E-4	0.0	0.0	12.07442532921178	0.0
15	1.798234493374405E-4	0.0	0.0	12.528119891890142	0.0
16	1.798234493374405E-4	0.0	0.0	13.199400828266807	0.0
17	1.798234493374405E-4	0.0	0.0	13.864747590815337	0.0
18	1.798234493374405E-4	0.0	0.0	14.622163959424636	0.0
19	1.798234493374405E-4	0.0	0.0	15.28805019232118	0.0
20	1.798234493374405E-4	0.0	0.0	15.808459254703731	0.0
21	1.798234493374405E-4	0.0	0.0	16.354043599993528	0.0
22	1.798234493374405E-4	0.0	0.0	16.92192605300116	0.0
23	1.798234493374405E-4	0.0	0.0	17.437120235352932	0.0
24	1.798234493374405E-4	0.0	0.0	17.87570962828695	0.0
25	3.59646898674881E-4	0.0	0.0	18.27887380170149	0.0
26	3.59646898674881E-4	0.0	0.0	18.663695983283613	0.0
27	3.59646898674881E-4	0.0	0.0	19.037369111006814	0.0
28	3.59646898674881E-4	0.0	0.0	19.41787552980484	0.0
29	3.59646898674881E-4	0.0	0.0	19.805934533475035	0.0
30	3.59646898674881E-4	0.0	0.0	20.233195049100793	0.0
31	3.59646898674881E-4	0.0	0.0	20.630425048687197	0.0
32	3.59646898674881E-4	0.0	0.0	21.030712046912342	0.0
33	3.59646898674881E-4	0.0	0.0	21.44592439143249	0.0
34	3.59646898674881E-4	0.0	0.0	21.84423333171492	0.0
35	3.59646898674881E-4	0.0	0.0	22.225459044310295	0.0
36	3.59646898674881E-4	0.0	0.0	22.600750583077534	0.0
37	3.59646898674881E-4	0.0	0.0	22.987191175703693	0.0
38	3.59646898674881E-4	0.0	0.0	23.403482460919868	0.0
39	3.59646898674881E-4	0.0	0.0	23.875698838879988	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATAGT	20	7.0301414E-4	45.000004	13
TCGTGCG	35	1.2104101E-7	45.000004	1
TCGAGCA	20	7.0301414E-4	45.000004	17
TTGGACG	25	3.8882004E-5	45.0	1
TGGATCG	25	3.8882004E-5	45.0	35
GTACGAG	25	3.8882004E-5	45.0	1
CGGGACG	30	2.1634733E-6	44.999996	6
TACGTAG	30	2.1634733E-6	44.999996	1
CATACGA	125	0.0	43.199997	18
CGTTTTT	2075	0.0	40.987953	1
CGATGAA	710	0.0	40.880283	19
TACGGGA	105	0.0	40.714287	4
GTTACGG	45	1.9252184E-8	40.000004	2
CGAGGGT	45	1.9252184E-8	40.000004	4
CTAGGGT	45	1.9252184E-8	40.000004	4
GCATATC	90	0.0	40.000004	28
GATGAAT	765	0.0	40.0	20
CGTGCGG	80	0.0	39.375004	2
TAGACGG	40	3.4539335E-7	39.375004	2
CGAATGC	80	0.0	39.375004	45
>>END_MODULE