##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551343_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560691
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.698143897440836	33.0	31.0	34.0	30.0	34.0
2	31.831754032078276	33.0	31.0	34.0	30.0	34.0
3	31.76177074360031	33.0	31.0	34.0	30.0	34.0
4	35.46286992300572	37.0	35.0	37.0	33.0	37.0
5	33.41971424545784	37.0	35.0	37.0	30.0	37.0
6	34.33305153819127	37.0	35.0	37.0	30.0	37.0
7	35.41166703228694	37.0	35.0	37.0	32.0	37.0
8	35.23944917967294	37.0	35.0	37.0	32.0	37.0
9	37.15247792456094	39.0	37.0	39.0	34.0	39.0
10	36.755514178041025	39.0	35.0	39.0	32.0	39.0
11	36.87351500202429	39.0	37.0	39.0	33.0	39.0
12	36.81560431681621	39.0	35.0	39.0	33.0	39.0
13	36.87782575429247	39.0	37.0	39.0	33.0	39.0
14	37.887317613444836	40.0	37.0	41.0	33.0	41.0
15	38.01817935369036	40.0	37.0	41.0	33.0	41.0
16	38.097306716177	40.0	37.0	41.0	34.0	41.0
17	37.990916565452274	40.0	37.0	41.0	33.0	41.0
18	37.949585422273586	39.0	37.0	41.0	33.0	41.0
19	37.8453943437651	39.0	37.0	41.0	33.0	41.0
20	37.98412137879866	40.0	36.0	41.0	34.0	41.0
21	37.84936444494383	40.0	36.0	41.0	33.0	41.0
22	38.00481727011848	40.0	36.0	41.0	34.0	41.0
23	38.09191158766593	40.0	36.0	41.0	34.0	41.0
24	37.96430297614907	40.0	36.0	41.0	34.0	41.0
25	37.62467562347175	39.0	35.0	41.0	33.0	41.0
26	37.723649924824905	40.0	36.0	41.0	33.0	41.0
27	37.3716271529238	40.0	35.0	41.0	33.0	41.0
28	37.41590466049928	40.0	35.0	41.0	33.0	41.0
29	37.64149593983139	40.0	36.0	41.0	33.0	41.0
30	37.374059865416065	39.0	35.0	41.0	33.0	41.0
31	37.31804148809237	39.0	35.0	41.0	33.0	41.0
32	37.38888086307788	40.0	35.0	41.0	33.0	41.0
33	37.395495914862195	40.0	35.0	41.0	33.0	41.0
34	37.25738062497882	40.0	35.0	41.0	33.0	41.0
35	37.22764053640954	40.0	35.0	41.0	33.0	41.0
36	37.02178383458982	40.0	35.0	41.0	32.0	41.0
37	37.01894448100647	40.0	35.0	41.0	32.0	41.0
38	36.86640948401169	39.0	35.0	41.0	31.0	41.0
39	36.81593426682433	39.0	35.0	41.0	31.0	41.0
40	36.71247799590149	39.0	35.0	41.0	31.0	41.0
41	36.568068686674124	39.0	35.0	41.0	31.0	41.0
42	36.70893415446297	39.0	35.0	41.0	31.0	41.0
43	36.621829135834176	39.0	35.0	41.0	31.0	41.0
44	36.70262943403764	39.0	35.0	41.0	31.0	41.0
45	36.68869484261385	39.0	35.0	41.0	31.0	41.0
46	36.57333540220906	39.0	35.0	41.0	31.0	41.0
47	36.51243019773815	39.0	35.0	41.0	31.0	41.0
48	36.474309378962744	39.0	35.0	41.0	31.0	41.0
49	36.435295019895094	39.0	35.0	41.0	31.0	41.0
50	36.356973805536384	39.0	35.0	41.0	31.0	41.0
51	35.47614639792685	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	26.0
10	47.0
11	47.0
12	32.0
13	44.0
14	53.0
15	65.0
16	91.0
17	109.0
18	185.0
19	293.0
20	478.0
21	809.0
22	1292.0
23	1836.0
24	2625.0
25	3768.0
26	4990.0
27	5792.0
28	6064.0
29	6635.0
30	8062.0
31	10501.0
32	14059.0
33	20783.0
34	37798.0
35	45783.0
36	45652.0
37	68658.0
38	114881.0
39	159177.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68123083837622	21.45941347373152	23.704321988403596	14.155033699488667
2	33.447478201005545	25.557392574519653	23.847181424349596	17.147947800125202
3	32.49008812340487	24.573606496269782	26.627679060302377	16.30862632002297
4	29.843532355611202	24.659571849735414	26.94122074368948	18.5556750509639
5	24.778353852656814	33.42001209222192	23.692729150280638	18.108904904840635
6	26.843484200745156	35.03444856436076	24.141817864028496	13.980249370865593
7	76.33866068832921	7.292965287475632	11.462998335981851	4.905375688213294
8	76.53003169303591	8.153332227554928	10.546629070200877	4.77000700920828
9	69.04034486018146	9.982860434713595	13.502802791555421	7.473991913549531
10	40.45418956252196	27.163268181583085	19.452960721680927	12.929581534214032
11	32.04510149083898	25.49140257289666	24.614448956733746	17.849046979530613
12	30.27032714989183	22.760129911127517	28.49840643063648	18.47113650834417
13	25.92194274564778	25.48944070798354	28.84726168245968	19.741354863908995
14	18.996737953703555	28.95569930674828	30.02866106286707	22.018901676681097
15	19.16296141725121	26.698662899886035	34.41200233283573	19.72637335002702
16	20.95646265055084	25.796918445275562	32.92740564767403	20.31921325649957
17	21.86302259176623	26.192145049590593	29.1085464186156	22.836285940027572
18	21.27606828003303	27.541908109814493	29.963206115311287	21.21881749484119
19	24.338361058051582	27.63875289598014	27.562775218435824	20.460110827532453
20	26.397427460044838	26.610378978795808	29.412992182860076	17.579201378299278
21	24.416478951864754	26.476615461992438	30.047209603863806	19.05969598227901
22	22.611384880442166	25.594846359224597	28.77645619423176	23.017312566101474
23	22.704840990848794	26.51210738178426	29.368582695281358	21.414468932085587
24	22.729453477940613	25.27827270278995	29.89204392437189	22.100229894897545
25	21.577125368518487	27.713660465390028	27.94872755225249	22.760486613838996
26	20.23520976794705	29.54996602406673	28.492699187252875	21.722125020733348
27	21.50953020469385	29.289394693333758	29.38356420916334	19.81751089280905
28	20.53038126169316	28.57919959478572	29.844424112389888	21.04599503113123
29	21.14622849305589	28.324870561503573	29.225188205268143	21.303712740172394
30	21.750661237651396	28.568498513441448	28.3325396698003	21.34830057910685
31	24.00716259044643	28.42991951003316	26.013080288429812	21.5498376110906
32	23.71859009686262	29.98407322393261	26.11099518272988	20.186341496474885
33	24.221897622754778	29.294031828582945	25.957791368151085	20.52627918051119
34	21.2999673617019	28.58562024359228	27.017555123945275	23.096857270760545
35	23.3488677364181	27.79231341327041	26.690637088877832	22.168181761433658
36	23.128068758014663	29.64324378311762	25.970989368475685	21.257698090392037
37	22.694853314927474	30.658241348621612	27.00007669108297	19.646828645367947
38	23.28198597801641	28.41297613123806	25.989894612183896	22.315143278561635
39	23.951873670167704	26.663884385517157	27.375862997622573	22.008378946692563
40	23.976129454548047	26.055706262451157	28.344310859279	21.6238534237218
41	20.855515783203227	26.773213766584448	28.265479560042877	24.10579089016945
42	22.407707632189567	27.374257835420934	28.716708490059588	21.50132604232991
43	23.547016092642828	26.94139909504522	27.834047630513066	21.677537181798886
44	22.509011202248654	27.686729410673617	26.96797344705016	22.836285940027572
45	22.992165024942437	27.009172610225598	26.064802181593784	23.933860183238185
46	22.486717282781424	28.496087863011894	26.774997280141825	22.24219757406486
47	21.995894351790916	27.374257835420934	28.896665007999054	21.73318280478909
48	21.88121443005149	26.09137653359872	29.597585836048733	22.42982320030106
49	21.31548392965109	25.83490728404772	30.57602137362647	22.27358741267472
50	20.510940963917736	26.817801605518905	28.928946603387608	23.742310827175753
51	20.593339290268613	25.174650565106273	27.761815331439244	26.47019481318587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	373.0
1	969.0
2	1565.0
3	3377.0
4	5189.0
5	3671.0
6	2153.0
7	2071.5
8	1990.0
9	1982.5
10	1975.0
11	1910.5
12	1846.0
13	1848.0
14	1850.0
15	1835.0
16	1820.0
17	1646.5
18	1473.0
19	1655.5
20	1838.0
21	2730.5
22	3623.0
23	3753.5
24	3884.0
25	4250.5
26	5581.5
27	6546.0
28	7446.0
29	8346.0
30	9942.0
31	11538.0
32	13498.0
33	15458.0
34	16098.0
35	16738.0
36	16929.0
37	17120.0
38	19078.5
39	21037.0
40	23374.0
41	25711.0
42	28869.0
43	32027.0
44	35061.0
45	38095.0
46	42095.0
47	46095.0
48	48060.5
49	50026.0
50	47857.0
51	45688.0
52	40541.5
53	35395.0
54	31973.5
55	28552.0
56	27057.5
57	25563.0
58	24365.0
59	23167.0
60	23563.5
61	23960.0
62	21467.5
63	18975.0
64	16309.5
65	13644.0
66	11123.5
67	8603.0
68	7308.5
69	6014.0
70	4767.0
71	3520.0
72	2896.5
73	2273.0
74	1717.5
75	933.5
76	705.0
77	532.5
78	360.0
79	214.0
80	68.0
81	49.0
82	30.0
83	42.0
84	54.0
85	38.0
86	22.0
87	12.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.9757985750113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68968457831046	20.895035713939418
2	10.088015447202942	5.644405763451042
3	3.7051360604721224	3.10962420362337
4	1.7773315514095687	1.9888907793298576
5	1.028407646967401	1.4385262592280674
6	0.7046005854417832	1.182705843249262
7	0.4919113399316162	0.9633128793883567
8	0.4283253281359695	0.9586194483606003
9	0.3369542798795992	0.8483908556609685
>10	5.760723535429205	41.23400240812185
>50	0.9166605402023819	15.879079862117914
>100	0.061927805671659855	3.0791219426002785
>500	0.006450813090797901	1.2823959983658175
>1k	0.003870487854478741	1.4958880425632226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2840	0.506517850295439	No Hit
GCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	1168	0.20831438350178616	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCC	1136	0.20260714011817563	No Hit
CCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGC	1101	0.19636484266735152	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1029	0.18352354505422772	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCG	1015	0.1810266260738981	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTC	993	0.17710289624766584	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	906	0.16158632829847455	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGC	900	0.1605162201640476	No Hit
CTGTCTCTTATACACATCTGACGCAAGAGCGTTCGTATGCCGTCTTCTGCT	726	0.12948308426566504	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	723	0.12894803019845155	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	671	0.11967375970008436	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	583	0.10397884039515526	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	566	0.10094686734761213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13626043578370262	0.0
2	0.0	0.0	0.0	0.7151889365087009	0.0
3	0.0	0.0	0.0	0.9871747540088927	0.0
4	0.0	0.0	0.0	1.3499414115796402	0.0
5	0.0	0.0	0.0	2.4635672768066548	0.0
6	0.0	0.0	0.0	3.0995682113677585	0.0
7	0.0	0.0	0.0	3.7054277668091693	0.0
8	0.0	0.0	0.0	4.530124435740898	0.0
9	0.0	0.0	0.0	4.85882598436572	0.0
10	0.0	0.0	0.0	5.780902493530305	0.0
11	0.0	0.0	0.0	7.037744497414797	0.0
12	0.0	0.0	0.0	8.1456631192582	0.0
13	0.0	0.0	0.0	8.578700210989654	0.0
14	0.0	0.0	0.0	8.770249567052083	0.0
15	0.0	0.0	0.0	9.10590681855068	0.0
16	0.0	0.0	0.0	9.710161211790451	0.0
17	0.0	0.0	0.0	10.39431701240077	0.0
18	0.0	0.0	0.0	11.120742084320954	0.0
19	0.0	0.0	0.0	11.583385501104887	0.0
20	0.0	0.0	0.0	12.042461890774062	0.0
21	0.0	0.0	0.0	12.547731281579336	0.0
22	0.0	0.0	0.0	13.084033808282994	0.0
23	0.0	0.0	0.0	13.569327847245631	0.0
24	0.0	0.0	0.0	13.985956614249202	0.0
25	0.0	0.0	0.0	14.357284136895366	0.0
26	0.0	0.0	0.0	14.686164036875926	0.0
27	0.0	0.0	0.0	15.038407964458141	0.0
28	0.0	0.0	0.0	15.392257054241998	0.0
29	0.0	0.0	0.0	15.723990575914362	0.0
30	0.0	0.0	0.0	16.1220708019212	0.0
31	0.0	0.0	0.0	16.478416810685385	0.0
32	0.0	0.0	0.0	16.86294233365615	0.0
33	0.0	0.0	0.0	17.211619234123607	0.0
34	0.0	0.0	0.0	17.56653843204189	0.0
35	0.0	0.0	0.0	17.925381359786407	0.0
36	0.0	0.0	0.0	18.283332530752233	0.0
37	0.0	0.0	0.0	18.66500443203119	0.0
38	0.0	0.0	0.0	19.01350298114291	0.0
39	0.0	0.0	0.0	19.388040828192356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	25	3.88824E-5	45.0	16
AACGTAT	20	7.0301926E-4	45.0	23
GTACCGG	50	2.1827873E-11	45.0	2
CCGATTA	20	7.0301926E-4	45.0	11
TCCGATC	20	7.0301926E-4	45.0	15
CGTTGTA	25	3.88824E-5	45.0	16
TTCACGG	35	1.2104465E-7	45.0	2
CGACCTA	20	7.0301926E-4	45.0	44
CACGCAT	20	7.0301926E-4	45.0	24
CGACCGT	20	7.0301926E-4	45.0	23
TTCAATC	25	3.88824E-5	45.0	40
GTTAGGC	20	7.0301926E-4	45.0	40
CGCGATT	25	3.88824E-5	45.0	17
CGTTAGA	20	7.0301926E-4	45.0	36
ATATCCG	20	7.0301926E-4	45.0	18
CGTAGAC	20	7.0301926E-4	45.0	12
CTACCGT	20	7.0301926E-4	45.0	13
GTTCACG	20	7.0301926E-4	45.0	23
CGTAAGG	50	2.1827873E-11	45.0	2
GTATACG	35	1.2104465E-7	45.0	1
>>END_MODULE