FastQCFastQC Report
Sat 18 Jun 2016
SRR3551339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975291
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80620.8266250790789621No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG51760.5307133973347442No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC44450.45576140864623993No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC42170.43238377058744515No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC17460.17902349145024407No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGTCGAT13740.1408810293543158No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12520.12837194232285545No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC12110.12416806881228269No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT10790.11063364677824361No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTACG207.03276E-445.023
TATAGCG302.165163E-644.9999961
CCGGTAT302.165163E-644.99999642
CGATCGA1950.042.69230741
TACGGGT800.042.18754
CCGATGA13350.040.95505518
TACGGCT4950.040.909097
CGTTTTT32400.040.902781
CGATGAA14000.040.33928719
CGAATGC1850.040.13513645
CGGTCTA451.927765E-840.031
GAGTACG356.2473173E-638.5714261
GTTACCG356.2473173E-638.57142638
ACGGCTG5350.037.8504688
CGAAAGG1250.037.82
TATTGCG301.13979506E-437.4999961
TGTTGCG601.546141E-1037.4999961
ATAAGCG301.13979506E-437.4999961
TACTAGC301.13979506E-437.49999635
TAAGACG301.13979506E-437.4999961