##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	975291
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.732085090501194	33.0	31.0	34.0	30.0	34.0
2	31.88410023264851	33.0	31.0	34.0	30.0	34.0
3	31.83122063056052	33.0	31.0	34.0	30.0	34.0
4	35.48973075728167	37.0	35.0	37.0	33.0	37.0
5	33.53066725725963	37.0	35.0	37.0	30.0	37.0
6	34.40182366083559	37.0	35.0	37.0	30.0	37.0
7	35.46649359011823	37.0	35.0	37.0	32.0	37.0
8	35.3737182030799	37.0	35.0	37.0	33.0	37.0
9	37.29905740953213	39.0	37.0	39.0	34.0	39.0
10	36.82100111658982	39.0	37.0	39.0	32.0	39.0
11	36.87608416359835	39.0	37.0	39.0	33.0	39.0
12	36.875698637637385	39.0	37.0	39.0	33.0	39.0
13	36.986299473695546	39.0	37.0	39.0	33.0	39.0
14	38.070355411871944	40.0	37.0	41.0	33.0	41.0
15	38.14359919244615	40.0	37.0	41.0	33.0	41.0
16	38.21211515332347	40.0	37.0	41.0	34.0	41.0
17	38.02498741401284	40.0	37.0	41.0	33.0	41.0
18	37.9993253295683	40.0	37.0	41.0	33.0	41.0
19	37.898877360705676	40.0	37.0	41.0	33.0	41.0
20	37.95489858924157	40.0	36.0	41.0	34.0	41.0
21	37.888794216290314	40.0	36.0	41.0	33.0	41.0
22	37.989159133017736	40.0	36.0	41.0	34.0	41.0
23	38.02135465209871	40.0	36.0	41.0	34.0	41.0
24	37.9206749575255	40.0	36.0	41.0	33.0	41.0
25	37.67126221814822	40.0	36.0	41.0	33.0	41.0
26	37.735977262170984	40.0	36.0	41.0	33.0	41.0
27	37.36244669539656	40.0	36.0	41.0	33.0	41.0
28	37.402913591943324	40.0	36.0	41.0	33.0	41.0
29	37.6413921588531	40.0	36.0	41.0	33.0	41.0
30	37.3670771082682	40.0	35.0	41.0	33.0	41.0
31	37.32361110683888	39.0	35.0	41.0	33.0	41.0
32	37.33086945332214	40.0	36.0	41.0	33.0	41.0
33	37.262123817404245	40.0	36.0	41.0	33.0	41.0
34	37.15106978327494	40.0	36.0	41.0	32.0	41.0
35	37.11817498572221	40.0	36.0	41.0	32.0	41.0
36	36.93096009293637	40.0	35.0	41.0	31.0	41.0
37	36.88777298262775	40.0	35.0	41.0	31.0	41.0
38	36.704626619132135	40.0	35.0	41.0	31.0	41.0
39	36.62513444705221	39.0	35.0	41.0	31.0	41.0
40	36.56128991244665	39.0	35.0	41.0	30.0	41.0
41	36.47580465727665	39.0	35.0	41.0	30.0	41.0
42	36.60591249175887	39.0	35.0	41.0	31.0	41.0
43	36.53832650972889	39.0	35.0	41.0	31.0	41.0
44	36.61370401244346	39.0	35.0	41.0	31.0	41.0
45	36.58607123412397	39.0	35.0	41.0	31.0	41.0
46	36.45440181443282	39.0	35.0	41.0	31.0	41.0
47	36.38076122921262	39.0	35.0	41.0	31.0	41.0
48	36.35846942092155	39.0	35.0	41.0	31.0	41.0
49	36.31384581627432	39.0	35.0	41.0	30.0	41.0
50	36.235361548501935	39.0	35.0	41.0	30.0	41.0
51	35.31268103571139	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	27.0
9	51.0
10	53.0
11	53.0
12	66.0
13	63.0
14	78.0
15	96.0
16	118.0
17	207.0
18	321.0
19	541.0
20	1235.0
21	2294.0
22	3868.0
23	4913.0
24	6158.0
25	7899.0
26	9873.0
27	10347.0
28	10777.0
29	11389.0
30	13652.0
31	17220.0
32	23097.0
33	33003.0
34	59229.0
35	72101.0
36	77757.0
37	113956.0
38	199125.0
39	295640.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38017166158613	20.008694840821867	22.697123217583265	14.914010280008736
2	32.24442756059474	27.205316156921374	24.241277731466816	16.30897855101708
3	30.80619015247757	25.668749122056905	28.015535875959074	15.509524849506457
4	28.093256269154537	24.452906875999062	29.9664407853656	17.487396069480802
5	26.408220725916674	32.65322862612287	23.862211381013463	17.076339266946995
6	25.97296601732201	35.87544640522675	24.48992146959215	13.66166610785909
7	76.87643995484424	6.028559681161828	13.0079125102149	4.087087853779026
8	78.91214006896404	8.545757112492579	9.065704492300247	3.4763983262431415
9	72.80575746110648	8.248409961744752	10.881982915868187	8.063849661280582
10	40.14032734845293	30.863608912621977	17.06383017991553	11.932233559009568
11	30.511406339236185	24.100396702112498	28.172001997352584	17.21619496129873
12	27.595866259403607	21.886698431545046	29.448133941562055	21.06930136748929
13	26.099287289639705	22.86650856000927	31.300196556719996	19.734007593631027
14	20.24995616692864	28.112737634203533	30.284192102664743	21.353114096203083
15	20.21776064784767	26.26539156005746	32.93222228032454	20.584625511770334
16	23.123047377654466	26.547871353267897	29.699648617694617	20.629432651383024
17	22.813191139875176	28.389988218900818	28.966329023850317	19.830491617373685
18	22.346663713701858	27.2057262909224	29.53682541928512	20.910784576090624
19	23.515750683642114	27.79970285791625	27.86645216658413	20.81809429185751
20	25.793634925370988	27.17650424334891	29.868623826119588	17.161237005160512
21	25.005972576390022	28.194456833909058	29.15673373382919	17.642836855871735
22	23.533386445686467	24.441628190970697	31.587700491443066	20.43728487189977
23	24.091783888090838	25.991934714869714	30.48956670368126	19.42671469335819
24	22.071258732009216	26.9966604838966	30.017707535494537	20.91437324859965
25	22.140263777682765	29.60449752945531	27.76996814284147	20.485270550020456
26	20.049195573423727	28.6828238956373	30.20595904196799	21.062021488970984
27	22.932335067174822	28.865641126597087	28.66118932708289	19.5408344791452
28	19.709297020068885	29.931169261276892	30.640188415560072	19.719345303094155
29	21.949551467203122	26.413449934429828	30.887089084181028	20.749909514186022
30	21.841481157931327	28.90306585419121	29.418296692987017	19.83715629489045
31	23.51595575064263	27.30118497966248	27.485847813626908	21.697011456067983
32	25.128295042197664	27.980674485871397	27.765969336331413	19.125061135599527
33	23.565787031767954	27.98487835938197	27.64067339901629	20.808661209833783
34	22.04080628243263	27.080532887107537	29.002420815941086	21.876240014518743
35	24.41486694740339	26.295433875633016	28.30427021268524	20.985428964278356
36	21.89520871206645	30.845665550076852	27.24673969102555	20.01238604683115
37	23.6822650880609	28.925110556746652	27.63062511599102	19.761999239201426
38	21.58114860077659	28.68323402963833	27.397053802403594	22.338563567181488
39	23.433826416936075	27.119290550205015	27.711318980693967	21.735564052164943
40	22.372502155766842	26.502038878652627	29.561946126848294	21.563512838732237
41	20.33854511115144	27.959860185319048	27.943762425778562	23.75783227775095
42	22.410952218363544	27.838973188515016	28.054293539056545	21.695781054064888
43	22.72378192765031	28.255361733062234	27.44380907852118	21.577047260766275
44	22.49779809308196	26.975846183344252	28.770284971357267	21.75607075221652
45	22.218291771379004	26.52992799072277	28.163491716831178	23.088288521067046
46	22.905676357107776	28.773053375864226	27.441348274514993	20.879921992513005
47	21.863525860486767	27.144001123767165	30.178274996898363	20.81419801884771
48	21.748073139196404	26.29512627513224	29.83683844103965	22.119962144631707
49	22.11052906260798	26.395916705885732	30.14741241332074	21.346141818185547
50	21.017624483359327	26.50388448165727	29.921223511751876	22.557267523231527
51	21.428578752392877	25.4655277245458	28.60961497645318	24.496278546608142
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	399.0
1	1341.5
2	2284.0
3	7783.5
4	13283.0
5	8616.0
6	3949.0
7	3791.0
8	3633.0
9	3633.5
10	3634.0
11	3575.0
12	3516.0
13	3428.0
14	3340.0
15	3276.0
16	3212.0
17	3263.0
18	3314.0
19	3447.0
20	3580.0
21	3786.5
22	3993.0
23	4343.0
24	4693.0
25	6021.5
26	9045.5
27	10741.0
28	13120.5
29	15500.0
30	18522.5
31	21545.0
32	23976.0
33	26407.0
34	29885.0
35	33363.0
36	34442.0
37	35521.0
38	38484.0
39	41447.0
40	44355.5
41	47264.0
42	51437.0
43	55610.0
44	57862.5
45	60115.0
46	73071.5
47	86028.0
48	82204.5
49	78381.0
50	76377.0
51	74373.0
52	68478.5
53	62584.0
54	57594.0
55	52604.0
56	50051.0
57	47498.0
58	45768.5
59	44039.0
60	41503.5
61	38968.0
62	34691.0
63	30414.0
64	24988.0
65	19562.0
66	16383.0
67	13204.0
68	10679.0
69	8154.0
70	6804.0
71	5454.0
72	4147.0
73	2840.0
74	2310.0
75	1306.5
76	833.0
77	677.5
78	522.0
79	316.0
80	110.0
81	168.0
82	226.0
83	117.5
84	9.0
85	9.0
86	9.0
87	5.0
88	1.0
89	2.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	975291.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.326954299471378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.282504517114	19.819590559718083
2	8.305935308239944	4.373399585487975
3	3.0525752579431806	2.410950279347015
4	1.640064483496528	1.7271161082079691
5	1.0870834440070662	1.4309798075042992
6	0.7456823176688854	1.1778926579515585
7	0.6302134416166779	1.1614120333448397
8	0.5495769438783656	1.1574949668423105
9	0.487544646433548	1.1552009063047155
>10	7.532486894746415	49.192415646723376
>50	0.6328985352721663	9.986745619365356
>100	0.044330603506073894	2.318132060771916
>500	0.005541325438259236	1.1123824394830621
>1k	0.002770662719129618	1.5968288995445847
>5k	7.916179197513196E-4	1.3794584294029766
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8062	0.8266250790789621	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG	5176	0.5307133973347442	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	4445	0.45576140864623993	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	4217	0.43238377058744515	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	1746	0.17902349145024407	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGTCGAT	1374	0.1408810293543158	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1252	0.12837194232285545	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	1211	0.12416806881228269	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	1079	0.11063364677824361	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08264200120784464	0.0
2	0.0	0.0	0.0	0.43074323458331926	0.0
3	0.0	0.0	0.0	0.5598329114079797	0.0
4	0.0	0.0	0.0	0.8054006445255826	0.0
5	0.0	0.0	0.0	1.6554033616633395	0.0
6	0.0	0.0	0.0	2.081224988234281	0.0
7	0.0	0.0	0.0	2.49412739377273	0.0
8	0.0	0.0	0.0	2.9841349915050994	0.0
9	0.0	0.0	0.0	3.18838172401878	0.0
10	0.0	0.0	0.0	4.2086925850848615	0.0
11	0.0	0.0	0.0	4.936065235914204	0.0
12	0.0	0.0	0.0	5.959349568487764	0.0
13	1.0253350025787175E-4	0.0	0.0	6.216195986633733	0.0
14	1.0253350025787175E-4	0.0	0.0	6.3298031049194545	0.0
15	1.0253350025787175E-4	0.0	0.0	6.571474565027258	0.0
16	1.0253350025787175E-4	0.0	0.0	6.877537063297006	0.0
17	1.0253350025787175E-4	0.0	0.0	7.241325922211935	0.0
18	1.0253350025787175E-4	0.0	0.0	7.605012247626606	0.0
19	1.0253350025787175E-4	0.0	0.0	8.030731340697288	0.0
20	1.0253350025787175E-4	0.0	0.0	8.305110987387355	0.0
21	1.0253350025787175E-4	0.0	0.0	8.595895994118678	0.0
22	1.0253350025787175E-4	0.0	0.0	8.954660711520972	0.0
23	1.0253350025787175E-4	0.0	0.0	9.255288934277052	0.0
24	1.0253350025787175E-4	0.0	0.0	9.502702270399297	0.0
25	1.0253350025787175E-4	0.0	0.0	9.727865836965583	0.0
26	1.0253350025787175E-4	0.0	0.0	9.958053545044505	0.0
27	1.0253350025787175E-4	0.0	0.0	10.204544079664428	0.0
28	2.050670005157435E-4	0.0	0.0	10.426324040722205	0.0
29	2.050670005157435E-4	0.0	0.0	10.665842297324593	0.0
30	2.050670005157435E-4	0.0	0.0	10.945348619027552	0.0
31	2.050670005157435E-4	0.0	0.0	11.200862101670168	0.0
32	2.050670005157435E-4	0.0	0.0	11.449198239294734	0.0
33	2.050670005157435E-4	0.0	0.0	11.693535570409242	0.0
34	2.050670005157435E-4	0.0	0.0	11.936950100021429	0.0
35	2.050670005157435E-4	0.0	0.0	12.193796518167398	0.0
36	2.050670005157435E-4	0.0	0.0	12.450848003313883	0.0
37	2.050670005157435E-4	0.0	0.0	12.709847624965267	0.0
38	2.050670005157435E-4	0.0	0.0	12.98525260665791	0.0
39	2.050670005157435E-4	0.0	0.0	13.256863848841013	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTACG	20	7.03276E-4	45.0	23
TATAGCG	30	2.165163E-6	44.999996	1
CCGGTAT	30	2.165163E-6	44.999996	42
CGATCGA	195	0.0	42.692307	41
TACGGGT	80	0.0	42.1875	4
CCGATGA	1335	0.0	40.955055	18
TACGGCT	495	0.0	40.90909	7
CGTTTTT	3240	0.0	40.90278	1
CGATGAA	1400	0.0	40.339287	19
CGAATGC	185	0.0	40.135136	45
CGGTCTA	45	1.927765E-8	40.0	31
GAGTACG	35	6.2473173E-6	38.571426	1
GTTACCG	35	6.2473173E-6	38.571426	38
ACGGCTG	535	0.0	37.850468	8
CGAAAGG	125	0.0	37.8	2
TATTGCG	30	1.13979506E-4	37.499996	1
TGTTGCG	60	1.546141E-10	37.499996	1
ATAAGCG	30	1.13979506E-4	37.499996	1
TACTAGC	30	1.13979506E-4	37.499996	35
TAAGACG	30	1.13979506E-4	37.499996	1
>>END_MODULE