##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	650383
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63919721148923	31.0	31.0	34.0	30.0	34.0
2	31.78988226937051	33.0	31.0	34.0	30.0	34.0
3	31.734153567974563	33.0	31.0	34.0	30.0	34.0
4	35.443615531156254	37.0	35.0	37.0	33.0	37.0
5	33.3301393179096	37.0	35.0	37.0	30.0	37.0
6	34.289823073481315	37.0	35.0	37.0	30.0	37.0
7	35.403709814063404	37.0	35.0	37.0	32.0	37.0
8	35.272614751615585	37.0	35.0	37.0	33.0	37.0
9	37.13776497848191	39.0	37.0	39.0	34.0	39.0
10	36.719208527898175	39.0	35.0	39.0	32.0	39.0
11	36.81517659594424	39.0	37.0	39.0	33.0	39.0
12	36.73562193353762	39.0	35.0	39.0	33.0	39.0
13	36.80952607924869	39.0	35.0	39.0	33.0	39.0
14	37.81550255772368	40.0	37.0	41.0	33.0	41.0
15	37.94424977282617	40.0	37.0	41.0	33.0	41.0
16	38.00001230044451	40.0	37.0	41.0	33.0	41.0
17	37.877679767152586	40.0	36.0	41.0	33.0	41.0
18	37.86853899932809	39.0	36.0	41.0	33.0	41.0
19	37.76589794013681	39.0	36.0	41.0	33.0	41.0
20	37.899740614376455	40.0	36.0	41.0	34.0	41.0
21	37.79652143429333	40.0	36.0	41.0	33.0	41.0
22	37.916716765352106	40.0	36.0	41.0	34.0	41.0
23	37.99825333687996	40.0	36.0	41.0	34.0	41.0
24	37.87701708070475	40.0	36.0	41.0	34.0	41.0
25	37.577859507397946	39.0	35.0	41.0	33.0	41.0
26	37.66184848004945	39.0	35.0	41.0	33.0	41.0
27	37.28385428278414	40.0	35.0	41.0	33.0	41.0
28	37.347290750219486	40.0	35.0	41.0	33.0	41.0
29	37.59670071327203	40.0	35.0	41.0	33.0	41.0
30	37.35040891290209	39.0	35.0	41.0	33.0	41.0
31	37.28676795057682	39.0	35.0	41.0	33.0	41.0
32	37.38723336864586	39.0	35.0	41.0	33.0	41.0
33	37.39503492557462	40.0	35.0	41.0	33.0	41.0
34	37.27044372316004	40.0	35.0	41.0	33.0	41.0
35	37.248222970157585	40.0	35.0	41.0	33.0	41.0
36	37.08228997375392	40.0	35.0	41.0	32.0	41.0
37	37.07465293527045	40.0	35.0	41.0	32.0	41.0
38	36.918598733361726	39.0	35.0	41.0	32.0	41.0
39	36.86645868665079	39.0	35.0	41.0	32.0	41.0
40	36.77416383884572	39.0	35.0	41.0	31.0	41.0
41	36.636887803032984	39.0	35.0	41.0	31.0	41.0
42	36.812564289041994	39.0	35.0	41.0	32.0	41.0
43	36.7171651165544	39.0	35.0	41.0	31.0	41.0
44	36.778404417089625	39.0	35.0	41.0	32.0	41.0
45	36.77554610129724	39.0	35.0	41.0	32.0	41.0
46	36.64570568418916	39.0	35.0	41.0	31.0	41.0
47	36.59449278348296	39.0	35.0	41.0	31.0	41.0
48	36.5764326558351	39.0	35.0	41.0	31.0	41.0
49	36.5470607319072	39.0	35.0	41.0	31.0	41.0
50	36.4738807748665	39.0	35.0	41.0	31.0	41.0
51	35.61756380471199	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	23.0
9	29.0
10	57.0
11	37.0
12	53.0
13	37.0
14	58.0
15	66.0
16	93.0
17	108.0
18	219.0
19	390.0
20	601.0
21	994.0
22	1523.0
23	2159.0
24	3008.0
25	4105.0
26	5266.0
27	6251.0
28	6976.0
29	8023.0
30	9705.0
31	12453.0
32	16954.0
33	24721.0
34	45090.0
35	54304.0
36	52744.0
37	77489.0
38	130511.0
39	186279.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1579546205851	20.859555062171058	22.324538002992085	13.657952314251757
2	33.1858305029498	25.25465764019047	24.48188221401851	17.07762964284122
3	32.47855494377928	24.58858857011945	26.517605779978876	16.415250706122393
4	30.50894626704573	24.621338503620173	26.925519270952652	17.944195958381446
5	24.63164012589505	33.230419614288806	23.996168411535972	18.141771848280168
6	26.95119644886167	34.5965377323823	24.442828302707788	14.009437516048237
7	76.44080487958634	7.1647014143973635	11.574564525825553	4.819929180190749
8	76.9778730378869	7.991598796401505	9.929995095197752	5.100533070513836
9	69.93187091298512	9.569130804464447	12.560137641974038	7.938860640576399
10	41.2527695219586	26.625388424974204	19.01556467496844	13.106277378098753
11	32.311883920705185	24.825987149110603	24.665927614959184	18.19620131522503
12	29.659446818259394	22.674178138112467	27.784705319788493	19.881669723839646
13	26.087859000004617	24.634407725909195	29.04503961511909	20.2326936589671
14	19.394572121350034	28.48352432336024	29.73109690751449	22.390806647775232
15	19.813402256824055	26.657677091805905	33.567605549345544	19.9613151020245
16	22.598991671061512	25.965623332713182	31.156564670355774	20.278820325869525
17	22.098209824057516	27.03468571595506	28.58592552388362	22.281178936103803
18	22.184774202277737	26.958422960009713	29.916218597349562	20.940584240362988
19	24.51355585862484	27.115714894146986	27.632794830123174	20.737934417105
20	25.382274751953847	27.196590316782572	29.276287971856586	18.144846959406998
21	24.96252208314178	27.419228362364944	28.87621601425622	18.74203354023706
22	22.717537205000745	25.55724857507038	29.784450085564966	21.940764134363906
23	22.48690387048862	26.497771313210833	29.67389984055549	21.34142497574506
24	22.545792248567384	25.45084973008212	30.033380331281723	21.969977690068777
25	21.12878104132488	28.654500502011892	28.05208623226622	22.16463222439701
26	20.076478013724223	28.63051463522263	28.556096945953385	22.736910405099763
27	21.529468021150613	29.21094186041148	29.602711017969412	19.656879100468494
28	19.129497542217432	29.410362816986297	30.264321176906527	21.19581846388974
29	20.831571550916923	27.481960629352244	29.871629486010548	21.814838333720285
30	22.637276804590524	27.7891642309224	28.3516020560193	21.221956908467778
31	22.444006070269364	28.646197701969456	26.679510380806388	22.230285846954796
32	23.411897297438585	28.17355312177594	27.135088094246008	21.279461486539468
33	23.919751900034285	27.453823362541762	27.03145684927189	21.59496788815206
34	20.890921195664706	27.397241317808124	28.352217078244664	23.359620408282506
35	22.33161075858379	26.636305069474446	27.625107052306102	23.40697711963566
36	22.55101993748299	29.64622384041403	26.706725114278818	21.09603110782416
37	22.16893737997457	28.80441216944477	28.723998013478212	20.302652437102445
38	21.491644154290626	28.49336467896609	26.984561404587758	23.03042976215553
39	22.74183058290269	26.020667821883414	29.14882461564955	22.088676979564347
40	22.906041517075323	25.18146999537196	28.398958767372456	23.513529720180262
41	19.447617788287825	26.376765690370135	28.182932210712764	25.992684310629276
42	20.487005349155805	26.492697379851567	28.98092354812472	24.03937372286791
43	22.078529112845814	26.412898246110366	28.4510819009722	23.05749074007162
44	21.44336490959942	27.047139916018715	27.495337362753947	24.014157811627918
45	21.655393821794235	26.373075557017945	27.41107931787885	24.560451303308973
46	22.00780155692876	28.1823171884874	26.956116626664596	22.85376462791924
47	20.495000638085557	26.161815422604835	30.800005535200025	22.54317840410958
48	20.73470555042183	25.5892297307894	30.155462243016807	23.520602475771966
49	20.91198570688348	25.322463840537036	31.0987525811714	22.66679787140808
50	20.11337934724616	25.171322128653422	30.220347087792888	24.49495143630753
51	19.85276367924746	24.107026167658134	28.138958121599117	27.90125203149529
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	363.0
1	1069.5
2	1776.0
3	3885.5
4	5995.0
5	4093.5
6	2192.0
7	2067.5
8	1943.0
9	1871.0
10	1799.0
11	1841.0
12	1883.0
13	1989.0
14	2095.0
15	1898.0
16	1701.0
17	1883.0
18	2065.0
19	1906.0
20	1747.0
21	3229.0
22	4711.0
23	4043.5
24	3376.0
25	4466.5
26	6940.5
27	8324.0
28	9088.5
29	9853.0
30	11212.5
31	12572.0
32	13938.0
33	15304.0
34	16621.5
35	17939.0
36	20049.5
37	22160.0
38	23585.0
39	25010.0
40	28370.5
41	31731.0
42	34024.5
43	36318.0
44	39007.0
45	41696.0
46	49082.0
47	56468.0
48	56623.5
49	56779.0
50	55812.5
51	54846.0
52	49571.0
53	44296.0
54	39141.5
55	33987.0
56	31619.0
57	29251.0
58	27971.5
59	26692.0
60	26063.5
61	25435.0
62	23357.5
63	21280.0
64	18503.5
65	15727.0
66	13042.5
67	10358.0
68	8859.5
69	7361.0
70	5837.5
71	4314.0
72	3356.0
73	2398.0
74	2064.5
75	1247.0
76	763.0
77	560.5
78	358.0
79	238.5
80	119.0
81	86.5
82	54.0
83	41.5
84	29.0
85	24.5
86	20.0
87	11.0
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	650383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.99761275383073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66915742094284	20.625605413725527
2	10.669226980436996	5.974257707619954
3	3.923338982961281	3.295323766408741
4	1.896676035288882	2.1240960462195644
5	1.1152373374563826	1.5611991551358522
6	0.7292241323138701	1.224992092036317
7	0.506886042915575	0.9934119397910326
8	0.43677631548375945	0.9782955312767438
9	0.3670307617141688	0.9248386621695137
>10	5.732003912593644	40.85505191406325
>50	0.883248263224603	15.142834605635565
>100	0.061738386157386804	3.0428311654852553
>500	0.004449613416748599	0.900547217502622
>1k	0.005005815093842174	2.3567147829300845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3251	0.4998593136659476	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	2049	0.31504513494356406	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCG	1830	0.2813726681047936	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGC	1587	0.24401006791382923	No Hit
CTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGCT	1549	0.2381673567728554	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTC	1379	0.21202891219481443	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	1246	0.19157942320140595	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCC	1228	0.18881182318726042	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1015	0.15606188968653856	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	959	0.1474515785314192	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	830	0.12761711176337634	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	784	0.12054435617167115	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	734	0.11285657835460028	No Hit
TCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	712	0.10947395611508912	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.075111126828346E-4	0.0	0.0	0.24585513458992625	0.0
2	3.075111126828346E-4	0.0	0.0	0.9663536716058077	0.0
3	3.075111126828346E-4	0.0	0.0	1.205597317273053	0.0
4	4.6126666902425185E-4	0.0	0.0	1.5546224301680702	0.0
5	4.6126666902425185E-4	0.0	0.0	2.6046191244236088	0.0
6	4.6126666902425185E-4	0.0	0.0	3.156294060576614	0.0
7	4.6126666902425185E-4	0.0	0.0	3.761629685892774	0.0
8	4.6126666902425185E-4	0.0	0.0	4.585144445657405	0.0
9	4.6126666902425185E-4	0.0	0.0	4.880355113832926	0.0
10	6.150222253656692E-4	0.0	0.0	5.925585385841881	0.0
11	6.150222253656692E-4	0.0	0.0	7.249420725941484	0.0
12	6.150222253656692E-4	0.0	0.0	8.494840732306963	0.0
13	6.150222253656692E-4	0.0	0.0	8.919513578921958	0.0
14	6.150222253656692E-4	0.0	0.0	9.107710379883853	0.0
15	6.150222253656692E-4	0.0	0.0	9.514086315294218	0.0
16	6.150222253656692E-4	0.0	0.0	10.166009874181828	0.0
17	6.150222253656692E-4	0.0	0.0	10.87251665557064	0.0
18	6.150222253656692E-4	0.0	0.0	11.649750992876506	0.0
19	6.150222253656692E-4	0.0	0.0	12.2025022179239	0.0
20	6.150222253656692E-4	0.0	0.0	12.703130309371556	0.0
21	7.687777817070864E-4	0.0	0.0	13.27110333449675	0.0
22	7.687777817070864E-4	0.0	0.0	13.882281670953883	0.0
23	7.687777817070864E-4	0.0	0.0	14.411354540324702	0.0
24	7.687777817070864E-4	0.0	0.0	14.888304276095777	0.0
25	7.687777817070864E-4	0.0	0.0	15.304981833781019	0.0
26	7.687777817070864E-4	0.0	0.0	15.675378969007493	0.0
27	7.687777817070864E-4	0.0	0.0	16.06130541542445	0.0
28	7.687777817070864E-4	0.0	0.0	16.444925528496285	0.0
29	7.687777817070864E-4	0.0	0.0	16.857297930603966	0.0
30	7.687777817070864E-4	0.0	0.0	17.296270043958714	0.0
31	7.687777817070864E-4	0.0	0.0	17.696803268228106	0.0
32	7.687777817070864E-4	0.0	0.0	18.123782448188223	0.0
33	7.687777817070864E-4	0.0	0.0	18.52154807244347	0.0
34	7.687777817070864E-4	0.0	0.0	18.92238880782554	0.0
35	7.687777817070864E-4	0.0	0.0	19.35167432113078	0.0
36	7.687777817070864E-4	0.0	0.0	19.763739212125778	0.0
37	7.687777817070864E-4	0.0	0.0	20.18487568094492	0.0
38	7.687777817070864E-4	0.0	0.0	20.576029816277487	0.0
39	7.687777817070864E-4	0.0	0.0	21.01146555183638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.031024E-4	45.000004	31
GGTACGA	20	7.031024E-4	45.000004	7
TCACCGC	20	7.031024E-4	45.000004	39
ATGTGCG	30	2.1640426E-6	45.000004	1
TAACGAT	20	7.031024E-4	45.000004	45
TAACGAC	20	7.031024E-4	45.000004	18
CGAATTG	20	7.031024E-4	45.000004	36
CGCAGTC	20	7.031024E-4	45.000004	42
AACGACC	20	7.031024E-4	45.000004	19
AATTCGC	20	7.031024E-4	45.000004	44
GCGATAT	40	6.8066583E-9	45.000004	9
ACGTACG	20	7.031024E-4	45.000004	38
TAGGACG	20	7.031024E-4	45.000004	1
GATTCGC	20	7.031024E-4	45.000004	29
CGATATT	20	7.031024E-4	45.000004	10
GGCAATC	25	3.8889317E-5	45.0	8
TACGACG	35	1.2108467E-7	45.0	1
TTCGGAT	25	3.8889317E-5	45.0	21
GCGTAAG	90	0.0	45.0	1
CGTAAGG	95	0.0	42.63158	2
>>END_MODULE