FastQCFastQC Report
Sat 18 Jun 2016
SRR3551337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241374
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78470.6321221485225242No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC35330.2846039952504241TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC30280.2439232656717476TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT24180.19478416657671258TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC21600.17400074433651744No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21220.17093962013059724No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC16290.13122556135379024No Hit
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC15150.1220421887360296TruSeq Adapter, Index 16 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC12470.10045320749427651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG302.1656433E-645.0000041
GCGTAGT253.8909864E-545.032
CGAATAT3750.041.39999814
CGGTCTA603.6379788E-1241.25000431
CGTTTTT31600.040.799051
CACGCTT451.9284926E-840.037
GTCGGGT451.9284926E-840.04
TACGGGC1200.039.3750044
CGCATCC403.4585173E-739.37512
GCGCAAC403.4585173E-739.3751
TCTACGG403.4585173E-739.3752
TACGAAT3950.039.30379512
CTACGAA4000.038.812511
TCGATCA356.2486924E-638.5714317
TCGTACG700.038.5714330
GCTACGA4050.038.33333610
TTACGGG5100.037.9411773
AATCCGT301.1399745E-437.50000440
TGCGATA301.1399745E-437.50000445
ACGGATC301.1399745E-437.5000045
Produced by FastQC (version 0.11.5)