##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551337_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1241374
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635965470518958	31.0	31.0	34.0	30.0	34.0
2	31.769983099372148	33.0	31.0	34.0	30.0	34.0
3	31.7186142129608	33.0	31.0	34.0	30.0	34.0
4	35.39207201053027	37.0	35.0	37.0	33.0	37.0
5	33.363204803709436	37.0	35.0	37.0	30.0	37.0
6	34.3101329655688	37.0	35.0	37.0	30.0	37.0
7	35.47380080459233	37.0	35.0	37.0	32.0	37.0
8	35.50198409182084	37.0	35.0	37.0	33.0	37.0
9	37.48296162155805	39.0	37.0	39.0	35.0	39.0
10	36.83286422947476	39.0	37.0	39.0	33.0	39.0
11	36.87359570927053	39.0	37.0	39.0	33.0	39.0
12	36.977045596250605	39.0	37.0	39.0	33.0	39.0
13	37.157842841883266	39.0	37.0	39.0	34.0	39.0
14	38.2164955927867	40.0	38.0	41.0	34.0	41.0
15	38.30344924253287	40.0	38.0	41.0	34.0	41.0
16	38.32142448609363	40.0	38.0	41.0	34.0	41.0
17	38.204762625929014	40.0	37.0	41.0	34.0	41.0
18	38.17213990304292	40.0	37.0	41.0	34.0	41.0
19	38.0766296055822	40.0	37.0	41.0	34.0	41.0
20	38.273961755280844	40.0	37.0	41.0	34.0	41.0
21	38.145244704657905	40.0	37.0	41.0	34.0	41.0
22	38.318494668004966	40.0	37.0	41.0	34.0	41.0
23	38.38582006711918	40.0	38.0	41.0	34.0	41.0
24	38.252235829008825	40.0	37.0	41.0	34.0	41.0
25	37.93062123099082	40.0	37.0	41.0	33.0	41.0
26	38.0891931037705	40.0	37.0	41.0	34.0	41.0
27	37.73177463036925	40.0	37.0	41.0	34.0	41.0
28	37.775232927385304	40.0	37.0	41.0	33.0	41.0
29	37.998704661125494	40.0	37.0	41.0	34.0	41.0
30	37.71245732551189	40.0	36.0	41.0	33.0	41.0
31	37.69451430431119	40.0	37.0	41.0	33.0	41.0
32	37.81928089359049	40.0	37.0	41.0	33.0	41.0
33	37.83809150183587	40.0	37.0	41.0	33.0	41.0
34	37.77873791460108	40.0	37.0	41.0	33.0	41.0
35	37.76201692640574	40.0	37.0	41.0	33.0	41.0
36	37.54959746216692	40.0	37.0	41.0	33.0	41.0
37	37.545949891007865	40.0	37.0	41.0	33.0	41.0
38	37.39335768269675	40.0	36.0	41.0	33.0	41.0
39	37.37446974078723	40.0	36.0	41.0	33.0	41.0
40	37.33218353211844	40.0	36.0	41.0	33.0	41.0
41	37.17886309846992	40.0	36.0	41.0	32.0	41.0
42	37.35370887419907	40.0	36.0	41.0	33.0	41.0
43	37.250565099639594	40.0	36.0	41.0	32.0	41.0
44	37.3169085223309	40.0	36.0	41.0	33.0	41.0
45	37.30052667447522	40.0	36.0	41.0	33.0	41.0
46	37.18369161912526	40.0	36.0	41.0	32.0	41.0
47	37.16409961864837	40.0	36.0	41.0	32.0	41.0
48	37.112940983136426	40.0	36.0	41.0	32.0	41.0
49	37.08106018009077	39.0	36.0	41.0	32.0	41.0
50	37.00854778656553	39.0	35.0	41.0	32.0	41.0
51	36.066936314116454	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	25.0
9	51.0
10	73.0
11	84.0
12	45.0
13	65.0
14	62.0
15	74.0
16	103.0
17	192.0
18	321.0
19	502.0
20	837.0
21	1357.0
22	1974.0
23	3082.0
24	4551.0
25	6757.0
26	8740.0
27	10632.0
28	11496.0
29	13319.0
30	15999.0
31	20684.0
32	27772.0
33	39360.0
34	65541.0
35	84487.0
36	100229.0
37	151892.0
38	272262.0
39	398676.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.24337065219668	20.95371741312449	25.75613795681237	14.046773977866462
2	31.30805059554977	24.777464325819615	26.716203174869136	17.19828190376148
3	30.720395303913246	23.05646807489121	30.22046538754638	16.002671233649167
4	28.66589762633985	25.558292666029736	28.29050713161384	17.485302576016576
5	23.85534093673623	33.70499140468545	25.29447209301951	17.145195565558808
6	25.62144849175188	34.49065309890492	25.125546370392804	14.762352038950388
7	80.7141119437011	5.36107571126832	9.487954476249703	4.436857868780883
8	82.89516294041924	4.648075438989378	8.302493849557022	4.1542677710343545
9	76.44811313915065	8.234826893426154	10.51778110384139	4.799278863581805
10	33.6327327622457	40.186277463520256	16.11657727646946	10.064412497764575
11	22.26500635586052	25.16727432667351	34.17382674359218	18.39389257387379
12	22.912031345911867	21.897510339349786	35.60780232226549	19.582655992472855
13	22.75663901451134	23.72234314557901	35.21388397050365	18.307133869405998
14	18.649657556868437	27.7422436751535	32.247171279566025	21.360927488412035
15	17.73824810250577	28.151709315645405	35.10207238108741	19.007970200761413
16	20.32377027390617	27.95201123915919	32.24652683236478	19.477691654569856
17	20.10788046148864	27.721460252913303	30.40687174050689	21.763787545091166
18	20.220417054006287	27.121560464453097	32.724303876188806	19.93371860535181
19	21.265629858527728	27.91527774868815	30.5342306186532	20.28486177413092
20	22.25227852363591	27.975130782503904	31.108755298564333	18.66383539529586
21	22.25203685593544	27.819416227502753	32.039659280764695	17.88888763579711
22	20.835461351695784	25.937308176262754	31.029810516411654	22.19741995562981
23	19.094003902127803	27.292016749182764	31.4537762189316	22.160203129757832
24	19.339457729902513	26.87393162737418	32.696914870135835	21.089695772587472
25	19.37949401227994	29.50939845687118	29.18556373824488	21.925543792604003
26	19.303046463032093	29.67784084409694	29.336444939236685	21.68266775363428
27	19.943224201570196	28.92287094783683	30.892946042046958	20.240958808546015
28	18.767510838796365	28.482713509385565	32.03820927456189	20.71156637725617
29	20.3714593667984	26.223442733616135	31.454501222033006	21.950596677552454
30	21.341110736973707	26.802075764435212	30.9025321941655	20.954281304425578
31	21.617981365809175	27.990194735833036	28.638750287987342	21.753073610370443
32	21.858279615973913	29.28368082463464	28.587516735488254	20.270522823903192
33	20.662105054560513	27.96022794097508	29.255164035979487	22.12250296848492
34	20.769324957667877	26.195328724461763	29.311553166088544	23.723793151781816
35	20.520407226186467	26.352332173865413	29.80793862284855	23.31932197709957
36	21.830971165821097	27.120835461351696	29.378736786818475	21.669456586008728
37	20.304356301968625	28.534752620886213	30.47582759104025	20.685063486104912
38	19.45521655842639	29.039274223561957	29.3859868178325	22.119522400179157
39	20.580824151303315	27.001854396821585	28.918198705627795	23.499122746247302
40	21.15140159210681	25.00245695495475	30.51336664051285	23.332774812425587
41	19.180762606595593	26.341134903743757	30.26090444942459	24.21719804023606
42	20.614738185268905	27.607070874692074	30.628722689535948	21.149468250503073
43	21.243074206484106	27.381917133756627	29.5806098726089	21.794398787150367
44	21.304538358302977	26.80715078614503	28.57841391877065	23.309896936781342
45	21.179354489460874	25.792065888281858	28.352776842434267	24.675802779823
46	21.184268399370374	27.044549023904157	28.747822976798286	23.023359599927176
47	20.04238851466198	25.702407171408453	31.62189638255675	22.63330793137282
48	19.896904558980612	25.622334606653595	31.171266677085228	23.30949415728056
49	20.512432192071046	25.429242113980155	31.27010876657639	22.788216927372414
50	19.80305693529911	24.876548083011244	30.96456023728546	24.355834744404184
51	19.7250788239483	24.263276015125175	28.98264342575243	27.02900173517409
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	500.0
1	1102.0
2	1704.0
3	6899.0
4	12094.0
5	7793.5
6	3493.0
7	3410.5
8	3328.0
9	3352.5
10	3377.0
11	3547.5
12	3718.0
13	3735.5
14	3753.0
15	3664.5
16	3576.0
17	3845.0
18	4114.0
19	4426.5
20	4739.0
21	5168.0
22	5597.0
23	6716.5
24	7836.0
25	9223.0
26	13941.0
27	17272.0
28	18492.0
29	19712.0
30	24047.5
31	28383.0
32	32250.5
33	36118.0
34	41320.0
35	46522.0
36	49546.5
37	52571.0
38	55877.0
39	59183.0
40	65262.5
41	71342.0
42	77183.0
43	83024.0
44	88621.0
45	94218.0
46	104784.0
47	115350.0
48	121727.5
49	128105.0
50	121942.5
51	115780.0
52	97802.0
53	79824.0
54	68372.5
55	56921.0
56	50085.0
57	43249.0
58	40269.5
59	37290.0
60	34704.0
61	32118.0
62	27733.5
63	23349.0
64	18450.0
65	13551.0
66	10912.5
67	8274.0
68	6526.5
69	4779.0
70	3793.5
71	2808.0
72	2225.0
73	1642.0
74	1288.5
75	610.5
76	286.0
77	256.0
78	226.0
79	138.0
80	50.0
81	38.5
82	27.0
83	22.0
84	17.0
85	11.5
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1241374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.05879819863183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.09111613399645	21.970311099845624
2	11.355456247100252	6.826627355699591
3	4.164303114378274	3.7552184085909173
4	2.102545063806467	2.5279991110595224
5	1.1825177401314113	1.7772531058456122
6	0.8043619782791871	1.450689263024782
7	0.5749041631264882	1.2096649756080693
8	0.4665165156629284	1.1218340640512663
9	0.36985097330430367	1.000554819310947
>10	4.975705487160908	36.850976824948276
>50	0.8565893758376972	16.113171782418945
>100	0.050465912259790024	2.678340226693055
>500	0.0032384542604925685	0.6488791672417277
>1k	0.002158969506995046	1.4319308738781067
>5k	2.6987118837438074E-4	0.6365489217834525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7847	0.6321221485225242	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	3533	0.2846039952504241	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	3028	0.2439232656717476	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	2418	0.19478416657671258	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC	2160	0.17400074433651744	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2122	0.17093962013059724	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1629	0.13122556135379024	No Hit
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	1515	0.1220421887360296	TruSeq Adapter, Index 16 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	1247	0.10045320749427651	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4166770046738534E-4	0.0	0.0	0.2010675267888646	0.0
2	2.4166770046738534E-4	0.0	0.0	0.9410540256199985	0.0
3	2.4166770046738534E-4	0.0	0.0	1.2653720796472296	0.0
4	2.4166770046738534E-4	0.0	0.0	1.6852294312592337	0.0
5	3.2222360062318043E-4	0.0	0.0	2.8549816574215345	0.0
6	3.2222360062318043E-4	0.0	0.0	3.7664716676843564	0.0
7	3.2222360062318043E-4	0.0	0.0	4.570983442540282	0.0
8	4.0277950077897555E-4	0.0	0.0	5.825158252065856	0.0
9	4.0277950077897555E-4	0.0	0.0	6.308896432501406	0.0
10	4.833354009347707E-4	0.0	0.0	7.4529513265140075	0.0
11	4.833354009347707E-4	0.0	0.0	9.066405450734429	0.0
12	4.833354009347707E-4	0.0	0.0	10.409997309432935	0.0
13	4.833354009347707E-4	0.0	0.0	10.95818020999312	0.0
14	4.833354009347707E-4	0.0	0.0	11.195578447752249	0.0
15	4.833354009347707E-4	0.0	0.0	11.522393734684309	0.0
16	5.638913010905657E-4	0.0	0.0	12.15266309750325	0.0
17	5.638913010905657E-4	0.0	0.0	13.08163373809988	0.0
18	5.638913010905657E-4	0.0	0.0	14.094463070758692	0.0
19	5.638913010905657E-4	0.0	0.0	14.708621253546474	0.0
20	6.444472012463609E-4	0.0	0.0	15.273559781339065	0.0
21	6.444472012463609E-4	0.0	0.0	15.948457112844316	0.0
22	6.444472012463609E-4	0.0	0.0	16.723485428243222	0.0
23	6.444472012463609E-4	0.0	0.0	17.42617454530222	0.0
24	6.444472012463609E-4	0.0	0.0	17.978063017269573	0.0
25	6.444472012463609E-4	0.0	0.0	18.420556576825355	0.0
26	6.444472012463609E-4	0.0	0.0	18.862969580480982	0.0
27	6.444472012463609E-4	0.0	0.0	19.327293788978984	0.0
28	6.444472012463609E-4	0.0	0.0	19.811193081214846	0.0
29	6.444472012463609E-4	0.0	0.0	20.324656388807885	0.0
30	6.444472012463609E-4	0.0	0.0	20.89523382961138	0.0
31	6.444472012463609E-4	0.0	0.0	21.431736124648978	0.0
32	6.444472012463609E-4	0.0	0.0	21.906452044267077	0.0
33	6.444472012463609E-4	0.0	0.0	22.397520811616804	0.0
34	6.444472012463609E-4	0.0	0.0	22.88786457586513	0.0
35	6.444472012463609E-4	0.0	0.0	23.408497358572035	0.0
36	6.444472012463609E-4	0.0	0.0	23.89940501412145	0.0
37	6.444472012463609E-4	0.0	0.0	24.39538769138068	0.0
38	6.444472012463609E-4	0.0	0.0	24.87219806440283	0.0
39	6.444472012463609E-4	0.0	0.0	25.352069561630902	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	30	2.1656433E-6	45.000004	1
GCGTAGT	25	3.8909864E-5	45.0	32
CGAATAT	375	0.0	41.399998	14
CGGTCTA	60	3.6379788E-12	41.250004	31
CGTTTTT	3160	0.0	40.79905	1
CACGCTT	45	1.9284926E-8	40.0	37
GTCGGGT	45	1.9284926E-8	40.0	4
TACGGGC	120	0.0	39.375004	4
CGCATCC	40	3.4585173E-7	39.375	12
GCGCAAC	40	3.4585173E-7	39.375	1
TCTACGG	40	3.4585173E-7	39.375	2
TACGAAT	395	0.0	39.303795	12
CTACGAA	400	0.0	38.8125	11
TCGATCA	35	6.2486924E-6	38.57143	17
TCGTACG	70	0.0	38.57143	30
GCTACGA	405	0.0	38.333336	10
TTACGGG	510	0.0	37.941177	3
AATCCGT	30	1.1399745E-4	37.500004	40
TGCGATA	30	1.1399745E-4	37.500004	45
ACGGATC	30	1.1399745E-4	37.500004	5
>>END_MODULE