FastQCFastQC Report
Sat 18 Jun 2016
SRR3551335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469409
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC33180.7068462683928088No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG32290.6878862569741951No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC28230.6013945194915309No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.4237243001305897No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC17950.38239573591473536No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC9510.20259517819215225No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCCAGT9460.20153000901133128No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC9230.19663023077955474No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT8870.1889610126776436No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT6590.14038929803220646No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTA5640.12015108359660766No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC5450.11610344070948789No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT5110.10886029027990515No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTC4830.10289534286730762No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT207.029017E-445.022
CTATGCG253.8872677E-545.01
GTACCTC253.8872677E-545.034
CGGCGAA351.2098826E-745.031
CGAACAC253.8872677E-545.034
TTGTGCG351.2098826E-745.01
GTACACG207.029017E-445.01
GCGAACA253.8872677E-545.033
TCCGACC207.029017E-445.029
CGACCCT207.029017E-445.012
GCACGAG253.8872677E-545.01
TAACGAA253.8872677E-545.024
GCGTTAG207.029017E-445.01
GCGCAAA207.029017E-445.030
CCGTCGA253.8872677E-545.041
CGGATCT207.029017E-445.06
CTACGCG207.029017E-445.01
TACGACC207.029017E-445.038
TACGAAG253.8872677E-545.01
CAAGGCG207.029017E-445.01