##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469409
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.715829905263853	33.0	31.0	34.0	30.0	34.0
2	31.85532233084581	33.0	31.0	34.0	30.0	34.0
3	31.8081374664738	33.0	31.0	34.0	30.0	34.0
4	35.49769177838516	37.0	35.0	37.0	33.0	37.0
5	33.446316538455804	37.0	35.0	37.0	30.0	37.0
6	34.36836958814168	37.0	35.0	37.0	30.0	37.0
7	35.49284525861243	37.0	35.0	37.0	32.0	37.0
8	35.41626172484976	37.0	35.0	37.0	33.0	37.0
9	37.33918821326391	39.0	37.0	39.0	34.0	39.0
10	36.84043339603629	39.0	37.0	39.0	33.0	39.0
11	36.88114203178891	39.0	37.0	39.0	33.0	39.0
12	36.903210206877155	39.0	37.0	39.0	33.0	39.0
13	36.996974919526465	39.0	37.0	39.0	33.0	39.0
14	38.048079606483896	40.0	37.0	41.0	33.0	41.0
15	38.16003740874163	40.0	37.0	41.0	33.0	41.0
16	38.25236627333519	40.0	37.0	41.0	34.0	41.0
17	38.09445281194012	40.0	37.0	41.0	33.0	41.0
18	38.09294453238008	40.0	37.0	41.0	34.0	41.0
19	38.015236179962464	40.0	37.0	41.0	34.0	41.0
20	38.092367210684074	40.0	37.0	41.0	34.0	41.0
21	38.02665479358087	40.0	37.0	41.0	34.0	41.0
22	38.15709115078748	40.0	37.0	41.0	34.0	41.0
23	38.178496790645255	40.0	37.0	41.0	34.0	41.0
24	38.085443611008735	40.0	37.0	41.0	34.0	41.0
25	37.83097256337224	40.0	36.0	41.0	33.0	41.0
26	37.916152012424135	40.0	37.0	41.0	34.0	41.0
27	37.540379498475744	40.0	36.0	41.0	33.0	41.0
28	37.602681243861966	40.0	36.0	41.0	33.0	41.0
29	37.889987196666446	40.0	37.0	41.0	33.0	41.0
30	37.63314082175672	40.0	36.0	41.0	33.0	41.0
31	37.61408281477347	40.0	36.0	41.0	33.0	41.0
32	37.638385714803086	40.0	37.0	41.0	33.0	41.0
33	37.63034155714952	40.0	37.0	41.0	33.0	41.0
34	37.53532846622029	40.0	37.0	41.0	33.0	41.0
35	37.509374553960406	40.0	37.0	41.0	33.0	41.0
36	37.34439902089649	40.0	36.0	41.0	33.0	41.0
37	37.28254890724294	40.0	36.0	41.0	33.0	41.0
38	37.174856042385215	40.0	36.0	41.0	32.0	41.0
39	37.110651904842044	40.0	36.0	41.0	32.0	41.0
40	37.067981227458354	40.0	36.0	41.0	32.0	41.0
41	36.98876672581906	40.0	35.0	41.0	31.0	41.0
42	37.082188453992146	40.0	36.0	41.0	32.0	41.0
43	36.99034530654504	40.0	36.0	41.0	32.0	41.0
44	37.128666046028094	40.0	36.0	41.0	32.0	41.0
45	37.132496394402324	40.0	36.0	41.0	33.0	41.0
46	36.99614834824215	40.0	35.0	41.0	32.0	41.0
47	36.928544190673804	39.0	35.0	41.0	32.0	41.0
48	36.923267342552016	39.0	35.0	41.0	32.0	41.0
49	36.936253885204586	39.0	35.0	41.0	32.0	41.0
50	36.82813069199781	39.0	35.0	41.0	32.0	41.0
51	36.01409431860063	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	16.0
10	26.0
11	19.0
12	16.0
13	23.0
14	22.0
15	26.0
16	45.0
17	70.0
18	102.0
19	236.0
20	484.0
21	939.0
22	1544.0
23	2030.0
24	2479.0
25	2952.0
26	3624.0
27	4018.0
28	4178.0
29	4673.0
30	5756.0
31	7680.0
32	10408.0
33	15322.0
34	26854.0
35	34091.0
36	36042.0
37	55825.0
38	100004.0
39	149838.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76718171147123	20.66279087107405	23.47249413624366	13.097533281211055
2	30.44360035704471	27.53014961366314	25.555752020093355	16.4704980091988
3	31.18304080237064	26.330130014550214	27.473269579407294	15.01355960367185
4	28.19524125016776	24.654618893118794	29.98280816942155	17.16733168729189
5	26.1153919076967	33.13954355370263	23.2157883636658	17.529276174934864
6	25.086012411351295	36.61284721852372	23.833586488541975	14.467553881583012
7	78.28354377525783	5.414041912276927	12.405173313677412	3.8972409987878374
8	79.21045399640825	8.43315743839594	8.34836997160259	4.008018593593221
9	73.18756138037405	8.189872797496427	10.16064881585142	8.461917006278107
10	37.371247675268265	35.67315496720344	16.345447147370418	10.61015021015788
11	27.495425098368376	23.35894710156814	31.351124499104195	17.794503300959292
12	24.954570534437984	21.963575474692647	32.348761953861136	20.733092037008237
13	23.60904882522491	23.818461086174317	34.29567818256574	18.276811906035036
14	17.876734361718672	30.394602574726942	31.06480702329951	20.663856040254874
15	17.327533132087368	27.3952991953712	36.158446045985485	19.118721626555946
16	19.989603948795185	27.04975831311287	31.813194889744338	21.147442848347602
17	19.86838769601776	28.693101325283493	29.87735642052027	21.561154558178476
18	19.719903112211313	27.65243103562139	31.858997164519643	20.768668687647658
19	21.54815949417246	27.457505075531145	29.897807668792037	21.096527761504362
20	24.23472920203916	27.617280452654295	31.785287457206827	16.36270288809972
21	22.56773943405431	29.36479701070921	30.45254777816361	17.614915777072873
22	21.84704596631083	24.480144181300314	31.903308202441792	21.769501649947063
23	20.73117473248276	28.015227658609014	30.856459931530928	20.397137677377298
24	20.435057700214525	27.142428031844297	31.76867081798602	20.653843449955154
25	19.290639932340454	30.828978566665743	28.922964834504665	20.95741666648914
26	18.223553446993986	30.36499087150012	31.252915900632498	20.158539780873397
27	20.69815448787731	30.32898815318837	30.074412718972155	18.898444639962165
28	17.423824426033587	30.010076500450566	32.849178435010835	19.716920638505016
29	20.276134458436033	25.24642688998294	33.477628251695215	20.999810399885813
30	19.491743873679457	28.2235747503776	32.06457481641809	20.22010655952485
31	21.727960051895042	27.79878528106619	27.77087784852868	22.702376818510082
32	23.941168575804895	29.19990882151812	27.97879887262494	18.880123730052045
33	21.84257225575138	27.5708390763705	27.690351058458617	22.896237609419504
34	20.005368452671338	27.044858534881094	29.89418609357724	23.055586918870326
35	21.096314727668194	26.74703723192355	30.1253278058154	22.03132023459286
36	21.306792157798423	28.2295396977902	29.9039856500408	20.55968249437058
37	20.454869846977797	28.3961321576706	31.17643675344955	19.97256124190205
38	20.201785649614727	29.866065627203568	26.630720757377897	23.301427965803807
39	23.22430971711237	26.225743434829752	27.903384894622814	22.646561953435064
40	20.06118331774636	26.037421523660605	30.02861044419685	23.872784714396186
41	19.285101052600183	27.21613773915711	29.277240104045728	24.22152110419698
42	20.986602302043632	28.02502721507257	29.904837785385453	21.083532697498345
43	22.628028009688776	26.363363292991828	29.405486473416577	21.603122223902822
44	20.834283109186234	26.24172097254207	30.279777337034442	22.644218581237258
45	20.53113596032458	26.508013267747316	28.678401990588164	24.28244878133994
46	23.016601726852276	27.655839577000012	28.071894659028697	21.255664037119015
47	19.41121708360939	26.620069065569684	32.03155457181264	21.937159279008284
48	20.616349494790256	25.507180305447914	29.889286315345466	23.987183884416364
49	20.42738848211261	24.586235031710086	32.37688242023481	22.609494065942492
50	20.155131239494768	25.54552639595747	30.52114467340848	23.778197691139283
51	19.62787249498838	25.460951963000284	27.973686060556997	26.937489481454342
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	583.0
2	919.0
3	2295.5
4	3672.0
5	2513.0
6	1354.0
7	1296.5
8	1239.0
9	1202.0
10	1165.0
11	1207.5
12	1250.0
13	1274.0
14	1298.0
15	1214.5
16	1131.0
17	1273.0
18	1415.0
19	1651.5
20	1888.0
21	2046.0
22	2204.0
23	2509.5
24	2815.0
25	3627.0
26	5759.0
27	7079.0
28	8138.0
29	9197.0
30	10502.0
31	11807.0
32	13558.0
33	15309.0
34	16073.5
35	16838.0
36	17721.0
37	18604.0
38	20422.5
39	22241.0
40	23968.5
41	25696.0
42	27945.0
43	30194.0
44	32708.5
45	35223.0
46	43184.5
47	51146.0
48	48108.0
49	45070.0
50	42524.5
51	39979.0
52	34112.0
53	28245.0
54	24564.5
55	20884.0
56	18956.0
57	17028.0
58	15944.5
59	14861.0
60	13168.0
61	11475.0
62	10511.5
63	9548.0
64	7592.5
65	5637.0
66	4725.5
67	3814.0
68	2822.5
69	1831.0
70	1469.0
71	1107.0
72	939.0
73	771.0
74	568.0
75	286.5
76	208.0
77	172.0
78	136.0
79	90.5
80	45.0
81	37.5
82	30.0
83	16.5
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	469409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.412530072470705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45043460197896	21.89775646639219
2	9.835258990407583	5.785597016518017
3	3.4586806368170526	3.0518564472436105
4	1.6866480106522985	1.9843434133993445
5	1.0337977536996021	1.5203303759771105
6	0.7363048173958019	1.2993952550495445
7	0.5834335903430322	1.201218061487779
8	0.45458706185333086	1.0696444501853755
9	0.41141436498004164	1.089066364399972
>10	6.954128916427016	49.00323082436112
>50	0.35439179092601236	6.238231537583584
>100	0.0314203031130279	1.7457849782881365
>500	0.005845637788470307	1.2865036168943476
>1k	0.0036535236177939415	2.827041192219888
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	3318	0.7068462683928088	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	3229	0.6878862569741951	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	2823	0.6013945194915309	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1989	0.4237243001305897	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	1795	0.38239573591473536	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	951	0.20259517819215225	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTAGCCAGT	946	0.20153000901133128	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC	923	0.19663023077955474	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	887	0.1889610126776436	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT	659	0.14038929803220646	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTA	564	0.12015108359660766	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	545	0.11610344070948789	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT	511	0.10886029027990515	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTC	483	0.10289534286730762	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19492596009024113	0.0
2	0.0	0.0	0.0	0.9929507103613266	0.0
3	0.0	0.0	0.0	1.2110973585934655	0.0
4	0.0	0.0	0.0	1.6341825572155626	0.0
5	0.0	0.0	0.0	3.124141207347963	0.0
6	0.0	0.0	0.0	3.7234053884778517	0.0
7	0.0	0.0	0.0	4.415765356011495	0.0
8	0.0	0.0	0.0	5.330106580828233	0.0
9	0.0	0.0	0.0	5.641349015464126	0.0
10	0.0	0.0	0.0	7.098926522499569	0.0
11	0.0	0.0	0.0	8.283181617736345	0.0
12	0.0	0.0	0.0	9.961888246710226	0.0
13	0.0	0.0	0.0	10.367717704603022	0.0
14	0.0	0.0	0.0	10.546666126980949	0.0
15	0.0	0.0	0.0	10.94354816375485	0.0
16	0.0	0.0	0.0	11.510857269460109	0.0
17	0.0	0.0	0.0	12.203430270829916	0.0
18	0.0	0.0	0.0	12.91709362197998	0.0
19	0.0	0.0	0.0	13.570894465167903	0.0
20	0.0	0.0	0.0	14.05085969804584	0.0
21	0.0	0.0	0.0	14.5866397959988	0.0
22	0.0	0.0	0.0	15.192934093722107	0.0
23	0.0	0.0	0.0	15.714440924652063	0.0
24	0.0	0.0	0.0	16.118140044183217	0.0
25	0.0	0.0	0.0	16.481362734843174	0.0
26	0.0	0.0	0.0	16.816891026801787	0.0
27	0.0	0.0	0.0	17.172657533196	0.0
28	0.0	0.0	0.0	17.518624483126654	0.0
29	0.0	0.0	0.0	17.88078200460579	0.0
30	0.0	0.0	0.0	18.31004518447665	0.0
31	0.0	0.0	0.0	18.70948362728452	0.0
32	0.0	0.0	0.0	19.089109923329122	0.0
33	0.0	0.0	0.0	19.439976651491556	0.0
34	0.0	0.0	0.0	19.796169225558096	0.0
35	0.0	0.0	0.0	20.158113713201068	0.0
36	0.0	0.0	0.0	20.528792588126773	0.0
37	0.0	0.0	0.0	20.893932583312207	0.0
38	0.0	0.0	0.0	21.2456514468193	0.0
39	0.0	0.0	0.0	21.59843547950721	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	7.029017E-4	45.0	22
CTATGCG	25	3.8872677E-5	45.0	1
GTACCTC	25	3.8872677E-5	45.0	34
CGGCGAA	35	1.2098826E-7	45.0	31
CGAACAC	25	3.8872677E-5	45.0	34
TTGTGCG	35	1.2098826E-7	45.0	1
GTACACG	20	7.029017E-4	45.0	1
GCGAACA	25	3.8872677E-5	45.0	33
TCCGACC	20	7.029017E-4	45.0	29
CGACCCT	20	7.029017E-4	45.0	12
GCACGAG	25	3.8872677E-5	45.0	1
TAACGAA	25	3.8872677E-5	45.0	24
GCGTTAG	20	7.029017E-4	45.0	1
GCGCAAA	20	7.029017E-4	45.0	30
CCGTCGA	25	3.8872677E-5	45.0	41
CGGATCT	20	7.029017E-4	45.0	6
CTACGCG	20	7.029017E-4	45.0	1
TACGACC	20	7.029017E-4	45.0	38
TACGAAG	25	3.8872677E-5	45.0	1
CAAGGCG	20	7.029017E-4	45.0	1
>>END_MODULE