FastQCFastQC Report
Sat 18 Jun 2016
SRR3551330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840803
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC135681.6136954792026192No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG115231.3704756048682034No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC99621.1848197496916637No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73410.8730939352024196No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT36480.4338709543139118No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC36390.4328005489989927No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT26720.3177914446071196No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT22710.2700989411312757No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT22390.2662930555671186No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTC18340.218124816395755No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTA16360.19457589946753281No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAAAACCGATCGAATGC9040.10751626718743867No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA207.0322043E-445.00000441
CGTCTAC207.0322043E-445.00000442
CCTCGTA650.045.00000440
CGTAATC207.0322043E-445.00000411
CTCGACG207.0322043E-445.0000041
TTACGCG207.0322043E-445.0000041
ATCGCGT207.0322043E-445.00000415
TCGGTAA207.0322043E-445.00000422
ATGACCG253.88991E-545.018
TCGACAC351.2114106E-745.034
CCGACCG302.1648048E-644.99999640
TCGAATA600.044.99999644
GCGTACG302.1648048E-644.9999961
CGATCGA8350.044.19161641
CGATGAA43900.043.76992819
CCGATCG9050.043.75690540
CCGATGA43000.043.4825618
ATCGAAT9700.043.3762943
CCGCGCC1200.043.12499640
CGTAAGC1050.042.85714343