##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551330_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	840803
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55839120459846	31.0	31.0	34.0	30.0	34.0
2	31.706838581689173	31.0	31.0	34.0	30.0	34.0
3	31.67340625568653	31.0	31.0	34.0	30.0	34.0
4	35.384926076619614	37.0	35.0	37.0	33.0	37.0
5	33.365598124649885	37.0	35.0	37.0	30.0	37.0
6	34.238879975452036	37.0	35.0	37.0	30.0	37.0
7	35.30935308270784	37.0	35.0	37.0	32.0	37.0
8	35.090201866549	37.0	35.0	37.0	32.0	37.0
9	36.74190268112745	39.0	37.0	39.0	32.0	39.0
10	36.439622598872745	38.0	35.0	39.0	32.0	39.0
11	36.60039985585208	39.0	35.0	39.0	32.0	39.0
12	36.64901290789876	39.0	35.0	39.0	32.0	39.0
13	36.694465885587945	39.0	35.0	39.0	32.0	39.0
14	37.80074167194932	40.0	37.0	41.0	33.0	41.0
15	37.82551322961502	40.0	37.0	41.0	33.0	41.0
16	37.99257019777522	40.0	37.0	41.0	33.0	41.0
17	37.666851807141505	40.0	36.0	41.0	32.0	41.0
18	37.688542976178724	39.0	36.0	41.0	33.0	41.0
19	37.57502173517459	39.0	36.0	41.0	32.0	41.0
20	37.31065659851356	39.0	35.0	41.0	31.0	41.0
21	37.441878775408746	39.0	35.0	41.0	32.0	41.0
22	37.49513976520065	39.0	36.0	41.0	32.0	41.0
23	37.31425316037169	40.0	35.0	41.0	32.0	41.0
24	37.376940852970314	39.0	35.0	41.0	32.0	41.0
25	37.32362872159115	39.0	35.0	41.0	32.0	41.0
26	37.176678722602084	39.0	35.0	41.0	31.0	41.0
27	36.640879016844615	39.0	35.0	41.0	30.0	41.0
28	36.6391069013788	39.0	35.0	41.0	30.0	41.0
29	36.893676640069074	39.0	35.0	41.0	30.0	41.0
30	36.71188970543635	39.0	35.0	41.0	30.0	41.0
31	36.619325811159094	39.0	35.0	41.0	30.0	41.0
32	36.43784453671074	39.0	35.0	41.0	30.0	41.0
33	36.21922138717393	39.0	35.0	41.0	27.0	41.0
34	36.0445062636551	40.0	35.0	41.0	27.0	41.0
35	35.89023944966895	40.0	35.0	41.0	25.0	41.0
36	35.81160033919955	39.0	35.0	41.0	25.0	41.0
37	35.502821707344054	39.0	35.0	41.0	23.0	41.0
38	35.56448300017959	39.0	35.0	41.0	24.0	41.0
39	35.28435792926524	39.0	35.0	41.0	23.0	41.0
40	35.48549065595627	39.0	35.0	41.0	23.0	41.0
41	35.248033130233836	39.0	35.0	41.0	23.0	41.0
42	35.281075352966155	39.0	35.0	41.0	23.0	41.0
43	35.26077927885605	39.0	35.0	41.0	23.0	41.0
44	35.48822613620551	39.0	35.0	41.0	23.0	41.0
45	35.47145288492072	39.0	35.0	41.0	23.0	41.0
46	35.32113229852891	39.0	35.0	41.0	23.0	41.0
47	35.130301628324354	39.0	35.0	41.0	22.0	41.0
48	35.243071206929564	39.0	35.0	41.0	23.0	41.0
49	35.24445559780353	39.0	35.0	41.0	23.0	41.0
50	35.0833108349994	39.0	35.0	41.0	22.0	41.0
51	34.3369647824758	38.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	33.0
10	33.0
11	37.0
12	44.0
13	49.0
14	37.0
15	83.0
16	102.0
17	146.0
18	379.0
19	834.0
20	2319.0
21	4916.0
22	8278.0
23	10942.0
24	12458.0
25	13175.0
26	13483.0
27	12412.0
28	11240.0
29	11170.0
30	13212.0
31	16374.0
32	21590.0
33	28905.0
34	46896.0
35	58302.0
36	63980.0
37	92984.0
38	159675.0
39	236615.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.71783640163035	17.737448605678143	20.189271446462488	14.355443546229022
2	29.48181678704762	33.570884023962805	22.162979913249593	14.784319275739977
3	27.557465898670674	32.7433417816064	26.006567531276648	13.692624788446285
4	24.529051394916525	22.099588131821605	37.137712401121306	16.23364807214056
5	30.94946140772571	29.51190706978924	23.30641065743105	16.232220865054
6	22.77679789439381	40.278043727246455	24.188424636924463	12.756733741435273
7	71.40864150104127	4.644369727510487	20.337344181692977	3.60964458975527
8	72.13972833113107	13.906824785354003	10.252342106296005	3.7011047772189207
9	66.68066122504321	7.058371580501022	11.076197397012141	15.184769797443634
10	39.93039986774548	31.36049704865468	17.379219626951855	11.329883456647991
11	30.43067163176154	21.995758816274442	30.54330205767582	17.030267494288196
12	23.522156795349208	19.81807867003329	31.75333579923002	24.906428735387482
13	26.90570799580877	20.975900419004216	35.10905646150168	17.009335123685336
14	18.127432942080368	32.13095100754874	29.689237550294184	20.052378500076713
15	16.59116344732357	25.33411512565964	34.831464683165976	23.243256743850818
16	19.073433372621174	25.703048157535118	29.54164055075921	25.681877919084496
17	18.860660582800016	31.30424130265948	30.301152588656322	19.53394552588418
18	18.841869022826987	25.62288669284006	30.179126382755534	25.35611790157742
19	19.55392642509601	26.926402498563874	28.260365388800945	25.259305687539175
20	27.50620537747843	25.32019985656569	31.540325141561105	15.633269624394774
21	22.11921222926179	31.92709826201857	29.43055626585538	16.52313324286426
22	20.103044351649554	22.203298513444885	36.163286762773204	21.530370372132353
23	24.757523462689836	26.25299862155582	30.94743953101975	18.042038384734592
24	20.715078323935572	29.042237004387474	29.479557042493905	20.763127629183053
25	18.65514276233553	35.01985601859175	27.24514541456203	19.079855804510686
26	18.873981182274562	26.369910668729773	33.93220528470998	20.82390286428569
27	25.041775540762817	28.407367718716515	27.397499771052196	19.153356969468472
28	17.294181871377717	32.10383407290412	31.125602548991854	19.47638150672631
29	20.470669110362355	23.802840855705796	33.78924670820632	21.93724332572553
30	18.359591961493955	33.56077464043302	29.11514349972586	18.964489898347175
31	20.85744223081982	26.80187868026161	26.722549753033707	25.618129335884866
32	28.559603141282796	27.560914982463192	25.48956176417068	18.38992011208333
33	24.805691701861196	25.88525492891914	26.182827606466674	23.126225762752988
34	20.33591697460642	25.036066712416584	29.186622787977683	25.441393524999317
35	25.81948446901355	26.494434487032038	26.29510123060931	21.3909798133451
36	18.989466022361956	34.236438261994785	25.5172733684347	21.256822347208562
37	21.27454350186667	28.996328509769825	28.905225124077816	20.82390286428569
38	19.683802269972873	28.50227698997268	25.539038276504723	26.274882463549726
39	22.664167468479537	28.234675661242882	27.951969724180337	21.149187146097244
40	21.7864351102458	24.91998720270979	28.25227788197711	25.041299805067297
41	18.359829829341713	28.74930274987125	28.338386042866166	24.552481377920866
42	23.390615875538025	26.404639374502707	29.126680090342212	21.078064659617056
43	21.538457878956187	28.845639228213983	26.281185961515362	23.33471693131447
44	20.964363828387864	26.298193512630185	29.641426112894457	23.09601654608749
45	20.382420138843464	25.591488136935762	28.107654230539143	25.918437493681633
46	24.462567331467657	28.64464089685693	25.72528880130066	21.16750297037475
47	18.43000084443086	28.872280427163084	31.729430080530157	20.968288647875898
48	20.838769604770675	27.245621150257556	28.881795141073475	23.033814103898298
49	21.62694471832284	24.08840120694146	32.456354223284166	21.82829985145153
50	20.567362390476724	26.064488352206165	30.07862721707701	23.289522040240104
51	19.333303996298774	24.469465499052692	29.304129504771037	26.8931009998775
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	283.0
1	765.0
2	1247.0
3	6782.5
4	12318.0
5	8109.5
6	3901.0
7	3811.5
8	3722.0
9	3525.0
10	3328.0
11	3330.5
12	3333.0
13	3176.5
14	3020.0
15	2997.0
16	2974.0
17	2866.5
18	2759.0
19	3028.5
20	3298.0
21	3616.0
22	3934.0
23	4127.0
24	4320.0
25	5329.5
26	8627.5
27	10916.0
28	12202.0
29	13488.0
30	14801.0
31	16114.0
32	19193.5
33	22273.0
34	25108.5
35	27944.0
36	29701.0
37	31458.0
38	32634.5
39	33811.0
40	38049.0
41	42287.0
42	44167.0
43	46047.0
44	50812.5
45	55578.0
46	80926.0
47	106274.0
48	87187.5
49	68101.0
50	66478.5
51	64856.0
52	58507.0
53	52158.0
54	48713.0
55	45268.0
56	41631.5
57	37995.0
58	34842.5
59	31690.0
60	30024.5
61	28359.0
62	24670.0
63	20981.0
64	16803.0
65	12625.0
66	9829.0
67	7033.0
68	5515.0
69	3997.0
70	3249.5
71	2502.0
72	2183.0
73	1864.0
74	1538.0
75	886.5
76	561.0
77	459.0
78	357.0
79	245.0
80	133.0
81	104.5
82	76.0
83	69.0
84	62.0
85	32.5
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	840803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.867237594647992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.13065040068697	21.976815733517142
2	8.122974922121434	4.689756941044934
3	2.963526235971484	2.5664644791528506
4	1.609114497338315	1.8580276204663084
5	1.135144517750506	1.6384243249082986
6	0.8594449605721687	1.4885881125815803
7	0.6682409817481699	1.3503189833423679
8	0.5698878728023973	1.3160870901196269
9	0.48494479906184207	1.2599115061326318
>10	7.1089013236088165	46.49392595961249
>50	0.30110357318168424	5.238790682920207
>100	0.03685273212499152	1.9861103498390942
>500	0.00460659151562394	0.8430904851042573
>1k	0.0029314673281243256	2.1686550347412203
>5k	8.375620937498073E-4	2.0917686641466826
>10k+	8.375620937498073E-4	3.033264032370364
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	13568	1.6136954792026192	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	11523	1.3704756048682034	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	9962	1.1848197496916637	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7341	0.8730939352024196	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	3648	0.4338709543139118	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	3639	0.4328005489989927	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT	2672	0.3177914446071196	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	2271	0.2700989411312757	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	2239	0.2662930555671186	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTC	1834	0.218124816395755	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTA	1636	0.19457589946753281	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAAAACCGATCGAATGC	904	0.10751626718743867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3786784775982009E-4	0.0	0.0	0.08265907709653747	0.0
2	2.3786784775982009E-4	0.0	0.0	0.6646027666409373	0.0
3	2.3786784775982009E-4	0.0	0.0	0.8312291939966913	0.0
4	2.3786784775982009E-4	0.0	0.0	1.4243526723858027	0.0
5	2.3786784775982009E-4	0.0	0.0	3.5342404820154067	0.0
6	2.3786784775982009E-4	0.0	0.0	4.209785169653296	0.0
7	2.3786784775982009E-4	0.0	0.0	4.816467115364717	0.0
8	2.3786784775982009E-4	0.0	0.0	5.491179265535447	0.0
9	2.3786784775982009E-4	0.0	0.0	5.697410689543211	0.0
10	2.3786784775982009E-4	0.0	0.0	7.588222211386021	0.0
11	2.3786784775982009E-4	0.0	0.0	8.190979337609404	0.0
12	2.3786784775982009E-4	0.0	0.0	9.971420178091657	0.0
13	2.3786784775982009E-4	0.0	0.0	10.24544393871097	0.0
14	2.3786784775982009E-4	0.0	0.0	10.383764092183306	0.0
15	2.3786784775982009E-4	0.0	0.0	10.740327995975276	0.0
16	2.3786784775982009E-4	0.0	0.0	11.069893899046507	0.0
17	2.3786784775982009E-4	0.0	0.0	11.444654693192103	0.0
18	2.3786784775982009E-4	0.0	0.0	11.839277452625645	0.0
19	2.3786784775982009E-4	0.0	0.0	12.462729081604133	0.0
20	2.3786784775982009E-4	0.0	0.0	12.75804201459795	0.0
21	2.3786784775982009E-4	0.0	0.0	13.047646119245531	0.0
22	2.3786784775982009E-4	0.0	0.0	13.395527846594268	0.0
23	2.3786784775982009E-4	0.0	0.0	13.704042445138754	0.0
24	2.3786784775982009E-4	0.0	0.0	13.948689526559729	0.0
25	2.3786784775982009E-4	0.0	0.0	14.178469867495716	0.0
26	2.3786784775982009E-4	0.0	0.0	14.414910508168976	0.0
27	2.3786784775982009E-4	0.0	0.0	14.683582242213694	0.0
28	2.3786784775982009E-4	0.0	0.0	14.91217324391088	0.0
29	2.3786784775982009E-4	0.0	0.0	15.15134936483338	0.0
30	2.3786784775982009E-4	0.0	0.0	15.44166707302424	0.0
31	2.3786784775982009E-4	0.0	0.0	15.682151467109419	0.0
32	2.3786784775982009E-4	0.0	0.0	15.94249782648254	0.0
33	2.3786784775982009E-4	0.0	0.0	16.173229638809566	0.0
34	2.3786784775982009E-4	0.0	0.0	16.42013646478426	0.0
35	3.568017716397301E-4	0.0	0.0	16.68298043655886	0.0
36	3.568017716397301E-4	0.0	0.0	16.93904517467231	0.0
37	3.568017716397301E-4	0.0	0.0	17.18690347203804	0.0
38	3.568017716397301E-4	0.0	0.0	17.440470597750007	0.0
39	3.568017716397301E-4	0.0	0.0	17.698914014341053	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	20	7.0322043E-4	45.000004	41
CGTCTAC	20	7.0322043E-4	45.000004	42
CCTCGTA	65	0.0	45.000004	40
CGTAATC	20	7.0322043E-4	45.000004	11
CTCGACG	20	7.0322043E-4	45.000004	1
TTACGCG	20	7.0322043E-4	45.000004	1
ATCGCGT	20	7.0322043E-4	45.000004	15
TCGGTAA	20	7.0322043E-4	45.000004	22
ATGACCG	25	3.88991E-5	45.0	18
TCGACAC	35	1.2114106E-7	45.0	34
CCGACCG	30	2.1648048E-6	44.999996	40
TCGAATA	60	0.0	44.999996	44
GCGTACG	30	2.1648048E-6	44.999996	1
CGATCGA	835	0.0	44.191616	41
CGATGAA	4390	0.0	43.769928	19
CCGATCG	905	0.0	43.756905	40
CCGATGA	4300	0.0	43.48256	18
ATCGAAT	970	0.0	43.37629	43
CCGCGCC	120	0.0	43.124996	40
CGTAAGC	105	0.0	42.857143	43
>>END_MODULE