##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392406
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.612671569751736	31.0	31.0	34.0	30.0	34.0
2	31.74729999031615	33.0	31.0	34.0	30.0	34.0
3	31.696378240903552	33.0	31.0	34.0	30.0	34.0
4	35.370532050988004	37.0	35.0	37.0	33.0	37.0
5	33.362565811939675	37.0	35.0	37.0	30.0	37.0
6	34.2880129253885	37.0	35.0	37.0	30.0	37.0
7	35.399119789197925	37.0	35.0	37.0	32.0	37.0
8	35.40066665647314	37.0	35.0	37.0	33.0	37.0
9	37.376423907891315	39.0	37.0	39.0	34.0	39.0
10	36.76831903691585	39.0	35.0	39.0	32.0	39.0
11	36.79064540297549	39.0	37.0	39.0	33.0	39.0
12	36.76367588670917	39.0	35.0	39.0	33.0	39.0
13	36.88612814279089	39.0	37.0	39.0	33.0	39.0
14	37.92297518386569	40.0	37.0	41.0	33.0	41.0
15	38.06428545944761	40.0	37.0	41.0	33.0	41.0
16	38.108683353465544	40.0	37.0	41.0	34.0	41.0
17	37.95628252371269	40.0	37.0	41.0	33.0	41.0
18	37.92608930546424	39.0	37.0	41.0	33.0	41.0
19	37.82463825731513	39.0	37.0	41.0	33.0	41.0
20	38.007510078846906	40.0	36.0	41.0	34.0	41.0
21	37.85535899043338	40.0	36.0	41.0	33.0	41.0
22	37.998544874441265	40.0	36.0	41.0	34.0	41.0
23	38.108196612691955	40.0	37.0	41.0	34.0	41.0
24	37.99917687293262	40.0	36.0	41.0	34.0	41.0
25	37.64578268426069	39.0	36.0	41.0	33.0	41.0
26	37.787572055473156	40.0	36.0	41.0	33.0	41.0
27	37.43080381033929	40.0	36.0	41.0	33.0	41.0
28	37.423497601973466	40.0	36.0	41.0	33.0	41.0
29	37.574083984444684	40.0	36.0	41.0	33.0	41.0
30	37.32932472999903	40.0	36.0	41.0	32.0	41.0
31	37.311254160232004	39.0	35.0	41.0	33.0	41.0
32	37.45291866077481	40.0	36.0	41.0	33.0	41.0
33	37.48298700835359	40.0	36.0	41.0	33.0	41.0
34	37.357953242305165	40.0	36.0	41.0	33.0	41.0
35	37.34054779998267	40.0	36.0	41.0	33.0	41.0
36	37.135145741910165	40.0	35.0	41.0	32.0	41.0
37	37.12259496541847	40.0	35.0	41.0	32.0	41.0
38	36.94317874854105	39.0	35.0	41.0	31.0	41.0
39	36.90980260240669	39.0	35.0	41.0	31.0	41.0
40	36.85114141985596	39.0	35.0	41.0	31.0	41.0
41	36.68436007604369	39.0	35.0	41.0	31.0	41.0
42	36.864385355983345	39.0	35.0	41.0	31.0	41.0
43	36.751209206790925	39.0	35.0	41.0	31.0	41.0
44	36.809679260765634	39.0	35.0	41.0	31.0	41.0
45	36.83305046304083	39.0	35.0	41.0	31.0	41.0
46	36.73381140961148	39.0	35.0	41.0	31.0	41.0
47	36.716314225572496	39.0	35.0	41.0	31.0	41.0
48	36.66909782215359	39.0	35.0	41.0	31.0	41.0
49	36.57596468963268	39.0	35.0	41.0	31.0	41.0
50	36.480543110961605	39.0	35.0	41.0	31.0	41.0
51	35.59737109014643	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	21.0
10	33.0
11	32.0
12	22.0
13	21.0
14	29.0
15	30.0
16	49.0
17	74.0
18	126.0
19	244.0
20	421.0
21	681.0
22	940.0
23	1302.0
24	1819.0
25	2632.0
26	3367.0
27	3916.0
28	4276.0
29	4792.0
30	5790.0
31	7425.0
32	9841.0
33	14116.0
34	24645.0
35	30863.0
36	31025.0
37	46256.0
38	80188.0
39	117387.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.49810655290694	20.86206632926102	24.501409254700487	14.138417863131552
2	31.58922136766512	25.0589950204635	26.788071538151815	16.563712073719568
3	31.669750207693053	23.651269348582847	28.823973129870595	15.855007313853509
4	28.10354581734224	25.221581729127486	28.46184818784626	18.213024265684012
5	24.24657115334628	33.49999745161899	25.29675896902698	16.956672426007756
6	25.667548406497353	34.90721344729693	24.627044438668115	14.798193707537601
7	79.9297156516465	5.824834482653171	9.756476710345916	4.488973155354403
8	81.39936698215624	5.957095457256005	8.546760243217484	4.096777317370274
9	75.47285209706274	8.517199023460394	10.57756507290918	5.432383806567687
10	38.24941514655739	34.91562310464162	16.53924761598956	10.295714132811424
11	27.991671890847748	24.707573278696042	29.81172561071951	17.4890292197367
12	27.54519553727517	21.955066945969225	31.860878783708714	18.638858733046895
13	25.04192086767277	24.520267274200698	31.516592508779173	18.92121934934736
14	18.83635826159641	28.894053607743004	31.444473326095927	20.82511480456466
15	17.89090890557229	27.496266621815163	35.96326253930878	18.649561933303772
16	20.835053490517474	26.494752883493117	33.12793382364184	19.54225980234757
17	20.47012532937825	26.910138988700478	29.53395208024342	23.085783601677853
18	22.66784911545695	26.4664658542428	30.131037751716335	20.734647278583914
19	22.73691024092394	27.59132123362028	29.128504660988874	20.543263864466905
20	24.74962156541949	26.914980912626206	30.24342135441354	18.09197616754076
21	24.39386757592901	27.36731854252993	30.140721599567794	18.098092281973262
22	21.977492698888394	25.460619868197732	30.23246331605531	22.329424116858558
23	21.716028806898976	26.176969771104417	30.17639893375738	21.93060248823922
24	21.238207366859836	25.6978741405585	31.1335708424438	21.93034765013787
25	19.986952289210663	28.407567672257816	29.02758877285261	22.577891265678915
26	20.064423072022343	29.07422414540043	29.23477214925358	21.62658063332365
27	21.206352604190556	28.98732435283864	29.690167836373554	20.11615520659725
28	20.450757633675327	28.348190394642288	31.138412766369527	20.062639205312866
29	21.599567794580103	25.93971549874365	31.823417582809643	20.637299123866608
30	23.322528197835915	27.08648695483759	29.46667482148591	20.124310025840582
31	23.101328725860462	28.590541429030136	27.358399208982533	20.94973063612687
32	23.784294837489742	29.15347879492159	27.910123698414395	19.152102669174273
33	24.17215842775085	27.862978649663866	28.033975015672542	19.93088790691274
34	22.661987839125803	26.968496913910595	29.34664607574808	21.02286917121553
35	22.1472148743903	26.822474681834628	29.62824217774448	21.40206826603059
36	24.17394229446033	27.514614965112667	28.749560404275165	19.561882336151843
37	22.581713837199228	28.972798581061454	29.34256866612641	19.10291891561291
38	21.59574522305979	28.658073525889005	27.816852953318755	21.92932829773245
39	24.055187739229268	27.200399586142925	27.816852953318755	20.927559721309052
40	23.114835145232234	25.794712619073103	30.003873539140585	21.086578696554078
41	20.873279205720603	26.71595235546857	29.501077965168726	22.909690473642094
42	22.59343638986152	26.193789085793796	29.31504615118016	21.89772837316453
43	23.417582809641036	25.787577152235187	29.305871979531407	21.488968058592377
44	21.851857514920773	26.984806552397263	28.70802179375443	22.45531413892754
45	21.239481557366606	27.045712858620917	28.08494263594338	23.629862948069093
46	22.297059677986574	28.14100701824131	28.170058561795692	21.391874741976423
47	21.92499605000943	26.212647105294007	30.3649791287595	21.497377715937066
48	21.334281331070372	24.910424407373995	30.881026284001774	22.87426797755386
49	21.970866908253186	25.352823351324904	31.34992839049352	21.32638134992839
50	20.395457765681464	25.956025137230316	31.163132062200884	22.485385034887337
51	21.209665499508162	24.74172158427751	29.42539104906653	24.6232218671478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	163.0
1	496.5
2	830.0
3	2089.5
4	3349.0
5	2286.0
6	1223.0
7	1230.5
8	1238.0
9	1228.5
10	1219.0
11	1160.5
12	1102.0
13	1100.0
14	1098.0
15	1122.0
16	1146.0
17	1191.5
18	1237.0
19	1298.5
20	1360.0
21	1483.0
22	1606.0
23	1922.5
24	2239.0
25	2492.5
26	3754.0
27	4762.0
28	5540.5
29	6319.0
30	7682.0
31	9045.0
32	10247.0
33	11449.0
34	12648.5
35	13848.0
36	15080.5
37	16313.0
38	16762.0
39	17211.0
40	18994.5
41	20778.0
42	23087.0
43	25396.0
44	27021.5
45	28647.0
46	31263.0
47	33879.0
48	34472.0
49	35065.0
50	34403.5
51	33742.0
52	28901.5
53	24061.0
54	21500.0
55	18939.0
56	17844.0
57	16749.0
58	15474.0
59	14199.0
60	14096.0
61	13993.0
62	12329.0
63	10665.0
64	8585.0
65	6505.0
66	5007.0
67	3509.0
68	3044.5
69	2580.0
70	2052.5
71	1525.0
72	1275.5
73	1026.0
74	954.0
75	651.0
76	420.0
77	333.0
78	246.0
79	152.0
80	58.0
81	45.0
82	32.0
83	19.5
84	7.0
85	3.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	392406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.91477501577507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36189772141879	24.37072785556708
2	9.159216927828018	5.846286951446195
3	3.182089765167567	3.046670368059703
4	1.6005525406805678	2.0432509694699
5	0.8742697296025023	1.3951060861683182
6	0.6536420257046084	1.2516502914730827
7	0.4931708464219209	1.1017605625526476
8	0.41015285737637175	1.0471948932195347
9	0.3714706000087942	1.0669860561830078
>10	6.582501903782631	50.272904059916
>50	0.27316293471708825	5.133551345307876
>100	0.033037405083777636	1.9848086004952505
>500	0.004028951839485077	0.9623158568350902
>1k	8.057903678970155E-4	0.47678610330632215
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1854	0.47246983991070474	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	990	0.25228972034066754	RNA PCR Primer, Index 47 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	797	0.2031059667793051	RNA PCR Primer, Index 17 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	701	0.17864150904930098	RNA PCR Primer, Index 17 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	700	0.17838667094794677	RNA PCR Primer, Index 17 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC	554	0.14118030815023214	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG	496	0.126399698271688	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC	446	0.11365779320397752	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT	404	0.10295459294710071	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18628665208992726	0.0
2	0.0	0.0	0.0	0.8233819054754514	0.0
3	0.0	0.0	0.0	1.0942748072149764	0.0
4	0.0	0.0	0.0	1.4459513870837857	0.0
5	0.0	0.0	0.0	2.40872973399999	0.0
6	0.0	0.0	0.0	3.0241637487704063	0.0
7	0.0	0.0	0.0	3.546581856546536	0.0
8	0.0	0.0	0.0	4.34473478998792	0.0
9	0.0	0.0	0.0	4.6204696156531755	0.0
10	0.0	0.0	0.0	5.441048302013731	0.0
11	0.0	0.0	0.0	6.573803662533193	0.0
12	0.0	0.0	0.0	7.593920582254094	0.0
13	0.0	0.0	0.0	8.135196709530435	0.0
14	2.5483810135420965E-4	0.0	0.0	8.405834773168605	0.0
15	2.5483810135420965E-4	0.0	0.0	8.673924455793234	0.0
16	2.5483810135420965E-4	0.0	0.0	9.226158621427807	0.0
17	5.096762027084193E-4	0.0	0.0	9.925434371543759	0.0
18	5.096762027084193E-4	0.0	0.0	10.6998873615592	0.0
19	5.096762027084193E-4	0.0	0.0	11.203447449835119	0.0
20	5.096762027084193E-4	0.0	0.0	11.644572203279257	0.0
21	5.096762027084193E-4	0.0	0.0	12.140232310413195	0.0
22	5.096762027084193E-4	0.0	0.0	12.727888972136002	0.0
23	5.096762027084193E-4	0.0	0.0	13.240368393959317	0.0
24	5.096762027084193E-4	0.0	0.0	13.646835165619281	0.0
25	5.096762027084193E-4	0.0	0.0	14.001569802704342	0.0
26	5.096762027084193E-4	0.0	0.0	14.334898039275648	0.0
27	5.096762027084193E-4	0.0	0.0	14.67765528559706	0.0
28	5.096762027084193E-4	0.0	0.0	15.018883503310347	0.0
29	5.096762027084193E-4	0.0	0.0	15.399356788632183	0.0
30	5.096762027084193E-4	0.0	0.0	15.800726798265062	0.0
31	5.096762027084193E-4	0.0	0.0	16.18145492168825	0.0
32	5.096762027084193E-4	0.0	0.0	16.550970168651855	0.0
33	5.096762027084193E-4	0.0	0.0	16.941891816129214	0.0
34	5.096762027084193E-4	0.0	0.0	17.326952187275424	0.0
35	5.096762027084193E-4	0.0	0.0	17.741573778178722	0.0
36	5.096762027084193E-4	0.0	0.0	18.14753087363598	0.0
37	5.096762027084193E-4	0.0	0.0	18.52902351136323	0.0
38	5.096762027084193E-4	0.0	0.0	18.918670968333817	0.0
39	5.096762027084193E-4	0.0	0.0	19.326666768601907	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	35	1.2091914E-7	45.000004	1
GCATATC	35	1.2091914E-7	45.000004	28
ACAACGT	20	7.0276024E-4	45.0	23
AGGTACG	20	7.0276024E-4	45.0	1
AGCCGGT	20	7.0276024E-4	45.0	32
CTCCGAG	25	3.8860937E-5	45.0	22
ATTAACG	25	3.8860937E-5	45.0	1
TGTGCCG	20	7.0276024E-4	45.0	1
CGCCTTG	20	7.0276024E-4	45.0	40
TCGTGCG	20	7.0276024E-4	45.0	1
TTGACGT	20	7.0276024E-4	45.0	20
TCGTCAG	20	7.0276024E-4	45.0	1
CACACCG	25	3.8860937E-5	45.0	17
CGCACGG	25	3.8860937E-5	45.0	2
TCGGTTA	25	3.8860937E-5	45.0	41
TCAACCC	20	7.0276024E-4	45.0	42
GATAGCG	20	7.0276024E-4	45.0	1
CGCCACT	20	7.0276024E-4	45.0	21
GCGTTAG	30	2.1618343E-6	44.999996	1
CTCGTAA	95	0.0	42.63158	33
>>END_MODULE