Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551327_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 990064 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 6464 | 0.652887086087364 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC | 3788 | 0.382601528790058 | Illumina PCR Primer Index 11 (95% over 21bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG | 2540 | 0.2565490715751709 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC | 2414 | 0.24382262156789863 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC | 1848 | 0.18665460010665977 | Illumina PCR Primer Index 11 (95% over 24bp) |
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT | 1491 | 0.15059632508605503 | No Hit |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 1229 | 0.12413338935664765 | No Hit |
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC | 1146 | 0.11575009292328577 | Illumina PCR Primer Index 11 (95% over 24bp) |
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 1138 | 0.11494206435139548 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGTCGTA | 25 | 3.890413E-5 | 45.0 | 11 |
TCTAGCG | 70 | 0.0 | 41.785717 | 1 |
CGTTTTT | 2535 | 0.0 | 40.650887 | 1 |
CTCGAAT | 50 | 1.0804797E-9 | 40.5 | 43 |
CGCTCGA | 45 | 1.927765E-8 | 40.0 | 41 |
ACGGGCT | 215 | 0.0 | 39.76744 | 5 |
TAATACG | 40 | 3.4575714E-7 | 39.375 | 4 |
TACGAAT | 195 | 0.0 | 39.23077 | 12 |
TTACGGG | 370 | 0.0 | 38.91892 | 3 |
CGTAAGG | 110 | 0.0 | 38.863636 | 2 |
ATTAGCG | 35 | 6.24741E-6 | 38.57143 | 1 |
GCGAAAT | 35 | 6.24741E-6 | 38.57143 | 19 |
GATCACG | 35 | 6.24741E-6 | 38.57143 | 1 |
ACTACGG | 70 | 0.0 | 38.57143 | 2 |
TTAGCGG | 170 | 0.0 | 38.382355 | 2 |
CCGATGA | 750 | 0.0 | 38.100002 | 18 |
GCGTTAG | 125 | 0.0 | 37.800003 | 1 |
CGATGAA | 790 | 0.0 | 37.594936 | 19 |
CCCCGCA | 30 | 1.1398078E-4 | 37.499996 | 42 |
TGCGTAG | 55 | 2.748493E-9 | 36.81818 | 1 |