FastQCFastQC Report
Sat 18 Jun 2016
SRR3551327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990064
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64640.652887086087364No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC37880.382601528790058Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG25400.2565490715751709No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC24140.24382262156789863No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC18480.18665460010665977Illumina PCR Primer Index 11 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT14910.15059632508605503No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12290.12413338935664765No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC11460.11575009292328577Illumina PCR Primer Index 11 (95% over 24bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11380.11494206435139548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTA253.890413E-545.011
TCTAGCG700.041.7857171
CGTTTTT25350.040.6508871
CTCGAAT501.0804797E-940.543
CGCTCGA451.927765E-840.041
ACGGGCT2150.039.767445
TAATACG403.4575714E-739.3754
TACGAAT1950.039.2307712
TTACGGG3700.038.918923
CGTAAGG1100.038.8636362
ATTAGCG356.24741E-638.571431
GCGAAAT356.24741E-638.5714319
GATCACG356.24741E-638.571431
ACTACGG700.038.571432
TTAGCGG1700.038.3823552
CCGATGA7500.038.10000218
GCGTTAG1250.037.8000031
CGATGAA7900.037.59493619
CCCCGCA301.1398078E-437.49999642
TGCGTAG552.748493E-936.818181