##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551327_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	990064
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635153889041515	31.0	31.0	34.0	30.0	34.0
2	31.781034357374878	33.0	31.0	34.0	30.0	34.0
3	31.73408789734805	33.0	31.0	34.0	30.0	34.0
4	35.42454124177831	37.0	35.0	37.0	33.0	37.0
5	33.40128415940788	37.0	35.0	37.0	30.0	37.0
6	34.29295277881026	37.0	35.0	37.0	30.0	37.0
7	35.39362303851064	37.0	35.0	37.0	32.0	37.0
8	35.36330883660046	37.0	35.0	37.0	33.0	37.0
9	37.286341085020766	39.0	37.0	39.0	34.0	39.0
10	36.70590184068909	39.0	35.0	39.0	32.0	39.0
11	36.77480950726418	39.0	37.0	39.0	33.0	39.0
12	36.80013615281436	39.0	35.0	39.0	33.0	39.0
13	36.93828782785759	39.0	37.0	39.0	33.0	39.0
14	37.985766576706155	40.0	37.0	41.0	33.0	41.0
15	38.093787876339306	40.0	37.0	41.0	33.0	41.0
16	38.131126876646356	40.0	37.0	41.0	33.0	41.0
17	37.976741907593855	40.0	37.0	41.0	33.0	41.0
18	37.930192391602965	40.0	37.0	41.0	33.0	41.0
19	37.84538777291165	39.0	37.0	41.0	33.0	41.0
20	37.97437034373535	40.0	37.0	41.0	34.0	41.0
21	37.84984304044991	40.0	36.0	41.0	33.0	41.0
22	37.994605399246915	40.0	37.0	41.0	34.0	41.0
23	38.066374497002215	40.0	37.0	41.0	34.0	41.0
24	37.94863261364922	40.0	36.0	41.0	33.0	41.0
25	37.61841961731767	39.0	36.0	41.0	33.0	41.0
26	37.72443801612825	40.0	36.0	41.0	33.0	41.0
27	37.34326972801758	40.0	36.0	41.0	33.0	41.0
28	37.35112578580779	40.0	36.0	41.0	32.0	41.0
29	37.54260431648863	40.0	36.0	41.0	33.0	41.0
30	37.30286021913735	39.0	35.0	41.0	32.0	41.0
31	37.297564601884325	39.0	35.0	41.0	32.0	41.0
32	37.38925867418672	40.0	36.0	41.0	33.0	41.0
33	37.38613665379208	40.0	36.0	41.0	33.0	41.0
34	37.28129191648217	40.0	36.0	41.0	33.0	41.0
35	37.246253777533575	40.0	36.0	41.0	32.0	41.0
36	37.02479132662131	40.0	35.0	41.0	32.0	41.0
37	37.019134116582364	40.0	35.0	41.0	32.0	41.0
38	36.866994456924	39.0	35.0	41.0	31.0	41.0
39	36.780369753874496	39.0	35.0	41.0	31.0	41.0
40	36.71803034955316	39.0	35.0	41.0	31.0	41.0
41	36.52516200972867	39.0	35.0	41.0	30.0	41.0
42	36.68414163124808	39.0	35.0	41.0	31.0	41.0
43	36.59454035294688	39.0	35.0	41.0	31.0	41.0
44	36.6645984502012	39.0	35.0	41.0	31.0	41.0
45	36.64427653161816	39.0	35.0	41.0	31.0	41.0
46	36.51050437143457	39.0	35.0	41.0	31.0	41.0
47	36.44104926550203	39.0	35.0	41.0	31.0	41.0
48	36.408264516233295	39.0	35.0	41.0	31.0	41.0
49	36.34716442573409	39.0	35.0	41.0	31.0	41.0
50	36.24430844874675	39.0	35.0	41.0	31.0	41.0
51	35.33376024176215	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	29.0
9	60.0
10	71.0
11	56.0
12	54.0
13	60.0
14	68.0
15	94.0
16	154.0
17	212.0
18	373.0
19	595.0
20	1189.0
21	1878.0
22	2793.0
23	3901.0
24	5224.0
25	6892.0
26	8775.0
27	10456.0
28	11137.0
29	12431.0
30	15020.0
31	18920.0
32	25029.0
33	35696.0
34	60777.0
35	75007.0
36	83198.0
37	121532.0
38	201959.0
39	286347.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.44809224454177	20.41807398309604	23.99006528870861	15.143768483653583
2	31.80854975031917	26.62676352235815	24.519930024725674	17.044756702597002
3	31.338378125050504	24.424986667528565	28.048287787456168	16.188347419964767
4	28.507248016289854	25.036361285735065	28.457049241261174	17.99934145671391
5	25.685612243248922	32.81989851161137	23.72149679212657	17.77299245301314
6	25.87307487192747	35.07843937361625	24.177830928101617	14.87065482635466
7	78.78500783787715	6.167480082095703	10.819704584754117	4.227807495273033
8	81.04910389631378	6.702192989544111	8.362792708350167	3.885910405791949
9	74.59224858190986	8.316937086895393	10.440941191680537	6.649873139514213
10	39.55623070831785	31.707748185975852	16.52953748444545	12.206483621260848
11	28.359479791205416	24.891825174938187	28.431091323389197	18.317603710467203
12	26.37839574007337	22.09988445191422	30.87143861406939	20.65028119394302
13	25.090903214337658	23.45898850983371	31.382718692932983	20.06738958289565
14	19.904470822088268	27.752448326572825	30.3874294995071	21.9556513518318
15	19.56408878617948	26.419908207954236	33.43763635482151	20.57836665104478
16	22.600963170057696	26.062759579178717	30.440557378108892	20.8957198726547
17	22.172304012669887	26.494044829425167	28.798340309313335	22.535310848591607
18	22.831655327332374	26.079021154188016	29.232352655989917	21.856970862489696
19	24.401553838943745	26.39061717222321	27.84304853019603	21.364780458637018
20	25.42583105738619	26.330015029331438	28.718648491410658	19.525505421871717
21	25.07706572504404	26.877959404644546	28.80036038074306	19.244614489568352
22	23.15577578823187	24.217323324552755	29.608590959776336	23.018309927439034
23	23.14900854894229	25.7896459218798	29.346688698912395	21.71465683026552
24	22.078269687616153	25.662583428950047	29.867362109924205	22.391784773509592
25	22.3151230627515	28.095557458911745	27.767598862295774	21.82172061604098
26	21.46618804440925	27.840624444480355	28.4774519627014	22.21573554840899
27	22.93760807382149	27.423580697813478	28.548457473456264	21.090353754908776
28	21.10843339420482	27.725682380128962	30.67185555681249	20.49402866885373
29	22.667524523667158	25.339473003765413	30.405610142374634	21.587392330192795
30	22.465113366408637	26.239111814993777	29.486780652563876	21.80899416603371
31	22.9368000452496	26.96421645469384	27.911528951663733	22.187454548392832
32	23.662611709950063	27.784365455162497	27.998493026713422	20.554529808174017
33	22.847209877341264	26.6764572795294	27.40661209780378	23.069720745325554
34	22.440367491394493	27.08925887619386	27.862441215921397	22.607932416490247
35	23.64362303851064	26.349003700770858	27.975565215986038	22.03180804473246
36	22.90963008451979	27.246622440569503	27.71790510512452	22.125842369786195
37	22.233613180562063	28.26706152329546	28.42695017695826	21.072375119184215
38	22.083420869761955	26.779985940302847	27.5316545193038	23.60493867063139
39	22.520968341440554	26.783319058161897	27.590135587194364	23.105577013203188
40	23.449797184828455	25.246751725141003	28.486643287706652	22.81680780232389
41	20.424134197385218	26.274564068585466	29.070140920182936	24.23116081384638
42	22.357746569918714	26.73241325813281	28.377155416215516	22.532684755732962
43	22.539148984308085	25.282405985875663	28.60764556634723	23.57079946346903
44	21.98322532684756	26.29779489002731	28.22383199469933	23.4951477884258
45	21.82131660175504	25.567741075324424	27.97687826241536	24.634064060505178
46	21.992012637566866	26.98482118327704	27.781335348017905	23.241830831138188
47	21.271756169298147	25.703489875401996	30.097246238626994	22.927507716672864
48	21.689708948108404	25.199078039399474	29.445672198968953	23.665540813523165
49	21.378213933644695	25.633292393219026	29.80413387417379	23.184359798962493
50	20.557862926033067	25.73348793613342	30.20410801725949	23.504541120574025
51	20.71825659755329	25.354522535916868	27.880419851645954	26.046801014883886
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	479.0
1	1237.5
2	1996.0
3	6366.0
4	10736.0
5	7028.5
6	3321.0
7	3151.0
8	2981.0
9	2926.0
10	2871.0
11	2745.5
12	2620.0
13	2622.0
14	2624.0
15	2644.5
16	2665.0
17	2632.5
18	2600.0
19	2707.0
20	2814.0
21	3334.5
22	3855.0
23	4388.0
24	4921.0
25	6349.0
26	9470.0
27	11163.0
28	12507.0
29	13851.0
30	16943.5
31	20036.0
32	22146.5
33	24257.0
34	27445.5
35	30634.0
36	33072.0
37	35510.0
38	37291.0
39	39072.0
40	43852.5
41	48633.0
42	52431.0
43	56229.0
44	60219.5
45	64210.0
46	74210.5
47	84211.0
48	82445.0
49	80679.0
50	79006.5
51	77334.0
52	68276.5
53	59219.0
54	56108.5
55	52998.0
56	49083.5
57	45169.0
58	44434.5
59	43700.0
60	42393.0
61	41086.0
62	37687.5
63	34289.0
64	30111.5
65	25934.0
66	21474.0
67	17014.0
68	14279.5
69	11545.0
70	9724.5
71	7904.0
72	6857.0
73	5810.0
74	4591.0
75	2715.5
76	2059.0
77	1564.0
78	1069.0
79	769.5
80	470.0
81	331.5
82	193.0
83	133.5
84	74.0
85	66.0
86	58.0
87	36.5
88	15.0
89	9.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	990064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.819263035996727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19819717003769	20.167602297359856
2	8.954162331194588	4.802880696546427
3	3.0732106205958694	2.472637319963381
4	1.6126136013616048	1.729964334013714
5	0.9584579858772247	1.2852568416096464
6	0.705356245716567	1.1350280812773448
7	0.5385222576102842	1.0109939054312302
8	0.45998500808400306	0.9869167139535968
9	0.4116078729733328	0.9935117831663047
>10	7.395249246289675	49.91963332095986
>50	0.6439844630579011	10.37946038516369
>100	0.03984198825931841	1.862329313673657
>500	0.005363344573369778	0.9874681139643331
>1k	0.003064768327639873	1.6021826832962032
>5k	3.830960409549841E-4	0.6641342096207938
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6464	0.652887086087364	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	3788	0.382601528790058	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	2540	0.2565490715751709	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	2414	0.24382262156789863	No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1848	0.18665460010665977	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1491	0.15059632508605503	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1229	0.12413338935664765	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1146	0.11575009292328577	Illumina PCR Primer Index 11 (95% over 24bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1138	0.11494206435139548	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0301071445886326E-4	0.0	0.0	0.09756945005575397	0.0
2	3.0301071445886326E-4	0.0	0.0	0.49633155028361803	0.0
3	3.0301071445886326E-4	0.0	0.0	0.6892483718224276	0.0
4	3.0301071445886326E-4	0.0	0.0	0.9907440327089966	0.0
5	3.0301071445886326E-4	0.0	0.0	1.9201788975258165	0.0
6	3.0301071445886326E-4	0.0	0.0	2.6157904943518804	0.0
7	3.0301071445886326E-4	0.0	0.0	3.1157581732090045	0.0
8	3.0301071445886326E-4	0.0	0.0	3.8373276879070444	0.0
9	3.0301071445886326E-4	0.0	0.0	4.1268039237867455	0.0
10	3.0301071445886326E-4	0.0	0.0	4.9366505599638	0.0
11	3.0301071445886326E-4	0.0	0.0	5.707509817547148	0.0
12	3.0301071445886326E-4	0.0	0.0	6.588664975193523	0.0
13	3.0301071445886326E-4	0.0	0.0	6.915108518237205	0.0
14	3.0301071445886326E-4	0.0	0.0	7.083683479047819	0.0
15	3.0301071445886326E-4	0.0	0.0	7.300942161314824	0.0
16	3.0301071445886326E-4	0.0	0.0	7.652333586515619	0.0
17	3.0301071445886326E-4	0.0	0.0	8.070387368897364	0.0
18	4.0401428594515103E-4	0.0	0.0	8.551568383458038	0.0
19	4.0401428594515103E-4	0.0	0.0	8.891849415795342	0.0
20	4.0401428594515103E-4	0.0	0.0	9.189607944536919	0.0
21	4.0401428594515103E-4	0.0	0.0	9.525141809014366	0.0
22	4.0401428594515103E-4	0.0	0.0	9.888956673507975	0.0
23	4.0401428594515103E-4	0.0	0.0	10.228429677273388	0.0
24	4.0401428594515103E-4	0.0	0.0	10.50376541314501	0.0
25	4.0401428594515103E-4	0.0	0.0	10.748194056141825	0.0
26	4.0401428594515103E-4	0.0	0.0	11.007874238433072	0.0
27	6.060214289177265E-4	0.0	0.0	11.260484170720277	0.0
28	6.060214289177265E-4	0.0	0.0	11.508548942290599	0.0
29	6.060214289177265E-4	0.0	0.0	11.768532135296304	0.0
30	6.060214289177265E-4	0.0	0.0	12.086188367620679	0.0
31	6.060214289177265E-4	0.0	0.0	12.380714782074694	0.0
32	6.060214289177265E-4	0.0	0.0	12.654333457231047	0.0
33	6.060214289177265E-4	0.0	0.0	12.943102668110344	0.0
34	6.060214289177265E-4	0.0	0.0	13.238639118279222	0.0
35	7.070250004040143E-4	0.0	0.0	13.544477932739701	0.0
36	7.070250004040143E-4	0.0	0.0	13.825166857900095	0.0
37	7.070250004040143E-4	0.0	0.0	14.10585578306049	0.0
38	7.070250004040143E-4	0.0	0.0	14.3871507296498	0.0
39	7.070250004040143E-4	0.0	0.0	14.692585529824335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGTA	25	3.890413E-5	45.0	11
TCTAGCG	70	0.0	41.785717	1
CGTTTTT	2535	0.0	40.650887	1
CTCGAAT	50	1.0804797E-9	40.5	43
CGCTCGA	45	1.927765E-8	40.0	41
ACGGGCT	215	0.0	39.76744	5
TAATACG	40	3.4575714E-7	39.375	4
TACGAAT	195	0.0	39.23077	12
TTACGGG	370	0.0	38.91892	3
CGTAAGG	110	0.0	38.863636	2
ATTAGCG	35	6.24741E-6	38.57143	1
GCGAAAT	35	6.24741E-6	38.57143	19
GATCACG	35	6.24741E-6	38.57143	1
ACTACGG	70	0.0	38.57143	2
TTAGCGG	170	0.0	38.382355	2
CCGATGA	750	0.0	38.100002	18
GCGTTAG	125	0.0	37.800003	1
CGATGAA	790	0.0	37.594936	19
CCCCGCA	30	1.1398078E-4	37.499996	42
TGCGTAG	55	2.748493E-9	36.81818	1
>>END_MODULE