##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	687001
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.580572662921888	31.0	31.0	34.0	30.0	34.0
2	31.74611245107358	31.0	31.0	34.0	30.0	34.0
3	31.704578304835074	31.0	31.0	34.0	30.0	34.0
4	35.4029659345474	37.0	35.0	37.0	33.0	37.0
5	33.30993404667533	37.0	35.0	37.0	30.0	37.0
6	34.26526744502555	37.0	35.0	37.0	30.0	37.0
7	35.374377912113665	37.0	35.0	37.0	32.0	37.0
8	35.195548478095375	37.0	35.0	37.0	32.0	37.0
9	37.0265487240921	39.0	37.0	39.0	33.0	39.0
10	36.651066010093146	38.0	35.0	39.0	32.0	39.0
11	36.786921707537545	39.0	37.0	39.0	33.0	39.0
12	36.79383727243483	39.0	35.0	39.0	33.0	39.0
13	36.83724768959579	39.0	37.0	39.0	33.0	39.0
14	37.89571485339905	40.0	37.0	41.0	33.0	41.0
15	37.94991273666268	40.0	37.0	41.0	33.0	41.0
16	38.07283832192384	40.0	37.0	41.0	33.0	41.0
17	37.88829565022467	40.0	37.0	41.0	33.0	41.0
18	37.87998852985658	39.0	37.0	41.0	33.0	41.0
19	37.77857674151857	39.0	37.0	41.0	33.0	41.0
20	37.76215755144461	39.0	36.0	41.0	33.0	41.0
21	37.738045505028374	39.0	36.0	41.0	33.0	41.0
22	37.84245292219371	40.0	36.0	41.0	33.0	41.0
23	37.83248932679865	40.0	36.0	41.0	33.0	41.0
24	37.77152580563929	40.0	36.0	41.0	33.0	41.0
25	37.52906473207462	39.0	35.0	41.0	33.0	41.0
26	37.568987526946835	39.0	36.0	41.0	33.0	41.0
27	37.126462698016454	40.0	35.0	41.0	32.0	41.0
28	37.16628651195558	40.0	35.0	41.0	32.0	41.0
29	37.42305178595082	40.0	36.0	41.0	32.0	41.0
30	37.17036074183298	39.0	35.0	41.0	32.0	41.0
31	37.143184653297446	39.0	35.0	41.0	32.0	41.0
32	37.089279346027155	39.0	35.0	41.0	32.0	41.0
33	37.056491911947724	40.0	35.0	41.0	32.0	41.0
34	36.85776876598433	40.0	35.0	41.0	31.0	41.0
35	36.79940349431806	40.0	35.0	41.0	31.0	41.0
36	36.629769097861576	40.0	35.0	41.0	30.0	41.0
37	36.549761936299944	39.0	35.0	41.0	30.0	41.0
38	36.3599790975559	39.0	35.0	41.0	30.0	41.0
39	36.35896017618606	39.0	35.0	41.0	30.0	41.0
40	36.2711538993393	39.0	35.0	41.0	30.0	41.0
41	36.142579122883376	39.0	35.0	41.0	29.0	41.0
42	36.201180202066666	39.0	35.0	41.0	30.0	41.0
43	36.128074049382754	39.0	35.0	41.0	29.0	41.0
44	36.233022950476055	39.0	35.0	41.0	30.0	41.0
45	36.267330032998494	39.0	35.0	41.0	30.0	41.0
46	36.21744364273123	39.0	35.0	41.0	30.0	41.0
47	36.114240008384265	39.0	35.0	41.0	29.0	41.0
48	36.10905078740788	39.0	35.0	41.0	29.0	41.0
49	36.0811599983115	39.0	35.0	41.0	29.0	41.0
50	35.97625330967495	39.0	35.0	41.0	29.0	41.0
51	35.10378150832386	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	21.0
10	31.0
11	33.0
12	32.0
13	32.0
14	36.0
15	50.0
16	73.0
17	125.0
18	237.0
19	562.0
20	1267.0
21	2112.0
22	3079.0
23	3889.0
24	4868.0
25	6080.0
26	7181.0
27	7964.0
28	8400.0
29	9023.0
30	10878.0
31	14149.0
32	18390.0
33	25349.0
34	42312.0
35	52844.0
36	56105.0
37	81478.0
38	139801.0
39	190518.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88785314722977	20.23126603891406	22.510593143241422	12.370287670614745
2	32.28452360331353	28.867498009464327	23.101421977551706	15.746556409670436
3	29.776812551946797	28.065606891401906	26.54741405034345	15.610166506307852
4	26.597923438248273	23.568670205720224	32.57666291606562	17.25674343996588
5	27.49006187763919	31.970113580620698	23.646544910414978	16.893279631325136
6	24.7797310338704	36.99921834174914	24.94727081911089	13.27377980526957
7	73.48417251212153	5.9388559841979855	16.10070436578695	4.47626713789354
8	73.7699071762632	10.868397571473695	10.730406505958506	4.63128874630459
9	68.29174921142764	8.006393003794754	12.39823522818744	11.303622556590165
10	37.758023641886986	30.964001508003626	18.787454457853773	12.490520392255615
11	29.05032161525238	22.716269699752985	30.588165082729134	17.6452436022655
12	25.108697076132348	20.168238474179805	32.62513446123077	22.097929988457075
13	24.14421521948294	24.14377853889587	34.45133267637165	17.26067356524954
14	18.8903655162074	30.020917000120818	29.972445454955672	21.116272028716114
15	17.30667058708794	25.97186903658073	36.19849170525225	20.52296867107908
16	19.138836770252155	25.663281421715546	31.391220682357083	23.80666112567522
17	19.12384407009597	27.476670339635607	31.484670327990788	21.91481526227764
18	19.739272577478054	25.96764779090569	32.00155458288998	22.291525048726278
19	20.876388826217138	25.71058848531516	30.074774272526533	23.33824841594117
20	23.646690470610668	25.871286941358164	33.304318334325565	17.1777042537056
21	22.014378436130368	29.14173341814641	31.218586290267407	17.625301855455813
22	20.267364967445463	23.370271658993218	33.48277513424289	22.87958823931843
23	20.581774990138296	27.29544789600015	31.324554112730553	20.798223001131003
24	20.737815519919184	26.294430430232268	31.61582006430849	21.35193398554005
25	18.852228744936326	31.090493318059213	28.769535997764194	21.287741939240263
26	18.664601652690465	27.201561569779376	32.17229669243567	21.96154008509449
27	21.709284265961767	28.766188113263297	29.00781803811057	20.516709582664365
28	16.997791851831366	28.969681266839498	32.900388791282694	21.13213809004645
29	21.192400011062578	24.77026962115048	31.724844650881153	22.312485716905798
30	19.605502757637908	30.037947543016674	29.265605144679558	21.090944554665857
31	21.890506709597222	28.381909196638723	26.199525182641654	23.5280589111224
32	22.884537285971927	30.69660742851903	26.52965570646913	19.889199579039914
33	22.73533808538852	28.09588341210566	26.163426254110256	23.005352248395564
34	23.012339137788736	27.578562476619396	27.525724125583512	21.883374260008356
35	22.03999703057201	27.94071624349892	26.87667121299678	23.142615512932295
36	24.451783912978293	29.526303455162367	26.11277130600974	19.9091413258496
37	19.790364206165638	29.653377506000716	28.804324884534378	21.75193340329927
38	19.200554293225192	32.25089919810888	25.853383037288157	22.69516347137777
39	22.86168433524842	29.98918487746015	27.271576023906807	19.877554763384627
40	21.133011451220597	29.004615713805364	28.61262210680916	21.24975072816488
41	21.872311685135827	26.773323474056077	27.705345407066368	23.649019433741728
42	21.4577562478075	27.555272845308814	29.208399987772943	21.77857091911074
43	24.160517961400345	26.293993749645196	27.21844655247955	22.32704173647491
44	21.169110379751995	26.1430478267135	29.33561959880699	23.352222194727517
45	20.80943113619922	27.05643805467532	27.52368628284384	24.610444526281622
46	24.127039116391387	28.646974312992267	26.765317663293064	20.460668907323278
47	19.39749723799529	27.661240667771953	32.21028790351106	20.730974190721703
48	22.001132458322477	26.774487955621606	28.95963761333681	22.264741972719108
49	20.383522003606984	25.162263228146685	32.800825617429965	21.653389150816373
50	21.300114555874007	24.976819538836185	29.913202455309378	23.809863449980423
51	20.562706604502758	24.430095443820317	28.340278980671062	26.666918971005867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	384.0
1	824.0
2	1264.0
3	4911.0
4	8558.0
5	5673.0
6	2788.0
7	2715.5
8	2643.0
9	2572.5
10	2502.0
11	2485.5
12	2469.0
13	2456.0
14	2443.0
15	2206.5
16	1970.0
17	2027.5
18	2085.0
19	2284.0
20	2483.0
21	2628.0
22	2773.0
23	3561.5
24	4350.0
25	5142.0
26	7192.5
27	8451.0
28	9564.0
29	10677.0
30	12754.5
31	14832.0
32	17469.0
33	20106.0
34	21613.5
35	23121.0
36	24055.0
37	24989.0
38	26380.5
39	27772.0
40	31193.5
41	34615.0
42	37547.0
43	40479.0
44	43952.5
45	47426.0
46	64475.0
47	81524.0
48	73591.0
49	65658.0
50	61792.0
51	57926.0
52	50555.0
53	43184.0
54	38479.5
55	33775.0
56	29899.5
57	26024.0
58	24551.0
59	23078.0
60	20552.5
61	18027.0
62	16803.0
63	15579.0
64	12946.5
65	10314.0
66	8206.0
67	6098.0
68	5286.5
69	4475.0
70	3608.5
71	2742.0
72	2086.5
73	1431.0
74	1276.5
75	823.5
76	525.0
77	422.5
78	320.0
79	178.0
80	36.0
81	25.0
82	14.0
83	12.0
84	10.0
85	16.5
86	23.0
87	12.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	687001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.326976980895623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99073562193435	20.219431291401335
2	10.949258059977343	5.984202459257734
3	4.0652912809737884	3.3327636376741916
4	2.000377315230129	2.186570593855981
5	1.1442557917762115	1.5634525841062514
6	0.7101778965498254	1.1644209018814773
7	0.5126128442175645	0.9805711575831372
8	0.39596618023315344	0.865644695395564
9	0.3151510051820377	0.7750911837704096
>10	4.726805962371521	33.8405500476957
>50	1.111835674879673	19.23405220067755
>100	0.06837924005313077	3.3537654301949535
>500	0.0021536768520671106	0.48627685999799597
>1k	0.004845772917151	2.123286343912581
>5k	0.0021536768520671106	3.8899206125951644
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG	7444	1.08355009672475	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC	7272	1.0585137430658762	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC	6635	0.9657918984106282	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5087	0.7404647154807635	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	3313	0.482240928324704	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAAGAGT	2045	0.2976706001883549	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT	1728	0.2515280181542676	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	1316	0.19155721752952326	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	1253	0.18238692520098224	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTA	1247	0.1815135640268355	No Hit
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	1205	0.17540003580780814	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTC	1177	0.17132435032845658	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTT	1147	0.16695754445772276	No Hit
CCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	937	0.13638990336258608	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	901	0.13114973631770552	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	777	0.11310027205200576	No Hit
TCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC	690	0.1004365350268777	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18748153205017168	0.0
2	0.0	0.0	0.0	1.028528342753504	0.0
3	0.0	0.0	0.0	1.2876254910837102	0.0
4	0.0	0.0	0.0	1.8077120702881073	0.0
5	0.0	0.0	0.0	3.658364398305097	0.0
6	0.0	0.0	0.0	4.336092669442984	0.0
7	0.0	0.0	0.0	5.119497642652631	0.0
8	0.0	0.0	0.0	6.069277919537235	0.0
9	0.0	0.0	0.0	6.360543871115181	0.0
10	0.0	0.0	0.0	8.237251474160882	0.0
11	0.0	0.0	0.0	9.43681304685146	0.0
12	0.0	0.0	0.0	11.524291813257914	0.0
13	0.0	0.0	0.0	11.98615431418586	0.0
14	0.0	0.0	0.0	12.201437843613038	0.0
15	0.0	0.0	0.0	12.685279934090344	0.0
16	0.0	0.0	0.0	13.274507606248026	0.0
17	0.0	0.0	0.0	13.999834061376912	0.0
18	0.0	0.0	0.0	14.703908727934893	0.0
19	0.0	0.0	0.0	15.520501425762117	0.0
20	0.0	0.0	0.0	16.04670153318554	0.0
21	0.0	0.0	0.0	16.593571188397107	0.0
22	0.0	0.0	0.0	17.21948002986895	0.0
23	0.0	0.0	0.0	17.779741223084102	0.0
24	0.0	0.0	0.0	18.224718741311875	0.0
25	0.0	0.0	0.0	18.617440149286537	0.0
26	0.0	0.0	0.0	19.044659323639994	0.0
27	0.0	0.0	0.0	19.455430195880357	0.0
28	0.0	0.0	0.0	19.866783308903482	0.0
29	0.0	0.0	0.0	20.280756505449045	0.0
30	0.0	0.0	0.0	20.73068307033032	0.0
31	0.0	0.0	0.0	21.159940087423454	0.0
32	0.0	0.0	0.0	21.579153451013898	0.0
33	0.0	0.0	0.0	21.99778457382158	0.0
34	0.0	0.0	0.0	22.411757770367146	0.0
35	0.0	0.0	0.0	22.807972623038395	0.0
36	0.0	0.0	0.0	23.21408556901664	0.0
37	0.0	0.0	0.0	23.60811701875252	0.0
38	0.0	0.0	0.0	24.011609881208326	0.0
39	0.0	0.0	0.0	24.4204884709047	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	20	7.031302E-4	45.000004	40
ATTCGCG	35	1.210974E-7	45.000004	1
ACGATTT	35	1.210974E-7	45.000004	38
CATAGCG	20	7.031302E-4	45.000004	1
CGAATTG	20	7.031302E-4	45.000004	44
TTTGCCG	20	7.031302E-4	45.000004	1
GGTCGAA	25	3.8891616E-5	45.0	42
CGTTAAG	25	3.8891616E-5	45.0	22
TACGAGA	25	3.8891616E-5	45.0	35
CGGTCGA	25	3.8891616E-5	45.0	41
AACACGT	25	3.8891616E-5	45.0	41
TCGTTAG	30	2.1642227E-6	44.999996	1
CCACGCT	30	2.1642227E-6	44.999996	43
CGATGAA	1130	0.0	43.00885	19
CCGATGA	1115	0.0	42.17489	18
TACGGCT	895	0.0	41.98324	7
ACGGCTG	915	0.0	41.557377	8
GTCGAAT	125	0.0	41.4	43
CGTATGG	60	3.6379788E-12	41.249996	2
TCGACGG	60	3.6379788E-12	41.249996	2
>>END_MODULE