FastQCFastQC Report
Sat 18 Jun 2016
SRR3551321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830220
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG134161.6159572161595719No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC116961.408783214087832No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC115991.3970995639709958No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106241.279660812796608No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC49880.6008046060080461No Hit
GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT36860.44397870443978704No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT30330.36532485365324857No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT30070.3621931536219315No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA24100.290284502902845No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC22020.26523090265230903No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC21060.25366770253667703No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT16090.1938040519380405No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC10480.12623160126231603No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10220.12309990123099901No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT9530.11478885114788852No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC9130.10997085109970851No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8570.1032256510322565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT302.1647702E-645.00000418
CGTATAT302.1647702E-645.00000422
CGTTCGG207.032153E-445.00000445
CCGTATA302.1647702E-645.00000421
TCCGTAT302.1647702E-645.00000420
CTACCCG207.032153E-445.00000434
CCGCGTA207.032153E-445.00000440
TCGACCG207.032153E-445.00000424
CGTCATA302.1647702E-645.00000438
GATTACG207.032153E-445.0000041
TCGAATA207.032153E-445.00000444
CCCTACG253.889866E-545.032
GTACTAG453.8380676E-1045.01
CGATGCA351.2113742E-745.010
GACGATA253.889866E-545.022
CGATGAA22950.042.94117719
CGTTTTT55400.042.8474731
CCGATGA22450.042.69487418
GATGAAT24950.042.6553120
CGTAAGC800.042.18750443