##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	830220
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.456317602563175	31.0	31.0	34.0	30.0	34.0
2	31.613549420635493	31.0	31.0	34.0	30.0	34.0
3	31.56357591963576	31.0	31.0	34.0	30.0	34.0
4	35.2979800534798	37.0	35.0	37.0	33.0	37.0
5	33.292521259425214	35.0	35.0	37.0	30.0	37.0
6	34.205139601551394	36.0	35.0	37.0	29.0	37.0
7	35.25033485100335	37.0	35.0	37.0	32.0	37.0
8	34.993606513936065	37.0	35.0	37.0	32.0	37.0
9	36.72342391173424	39.0	37.0	39.0	32.0	39.0
10	36.365264628652646	38.0	35.0	39.0	32.0	39.0
11	36.59832333598323	38.0	35.0	39.0	32.0	39.0
12	36.61865890968659	39.0	35.0	39.0	32.0	39.0
13	36.654125412541255	39.0	35.0	39.0	32.0	39.0
14	37.69663462696634	39.0	37.0	41.0	33.0	41.0
15	37.74665148996652	40.0	37.0	41.0	33.0	41.0
16	37.87871287128713	40.0	37.0	41.0	33.0	41.0
17	37.61686540916865	39.0	36.0	41.0	32.0	41.0
18	37.60987087759871	39.0	36.0	41.0	33.0	41.0
19	37.42699886776999	39.0	36.0	40.0	32.0	41.0
20	37.27904169979042	39.0	35.0	41.0	32.0	41.0
21	37.359109633591096	39.0	35.0	41.0	32.0	41.0
22	37.47756377827564	39.0	35.0	41.0	33.0	41.0
23	37.38559056635591	39.0	35.0	41.0	32.0	41.0
24	37.314746693647464	39.0	35.0	41.0	32.0	41.0
25	37.17450796174508	39.0	35.0	41.0	32.0	41.0
26	37.16353858013539	39.0	35.0	41.0	32.0	41.0
27	36.69907735299078	39.0	35.0	41.0	31.0	41.0
28	36.7350593818506	39.0	35.0	41.0	31.0	41.0
29	36.941947917419476	39.0	35.0	41.0	31.0	41.0
30	36.65191274601913	39.0	35.0	41.0	31.0	41.0
31	36.603476187034765	39.0	35.0	40.0	30.0	41.0
32	36.402841415528414	39.0	35.0	41.0	30.0	41.0
33	36.158608561586085	39.0	35.0	41.0	29.0	41.0
34	35.84851364698514	39.0	35.0	41.0	27.0	41.0
35	35.75098407650984	39.0	35.0	41.0	26.0	41.0
36	35.48865963238659	39.0	35.0	41.0	25.0	41.0
37	35.3546903230469	39.0	35.0	41.0	24.0	41.0
38	35.25576594155766	39.0	35.0	40.0	23.0	41.0
39	35.00163932451639	39.0	35.0	40.0	23.0	41.0
40	34.85010840500109	38.0	34.0	40.0	23.0	41.0
41	34.658125557081256	38.0	34.0	40.0	22.0	41.0
42	34.719693575196935	38.0	34.0	40.0	23.0	41.0
43	34.56585483365855	37.0	34.0	40.0	22.0	41.0
44	34.641781696417816	37.0	34.0	40.0	23.0	41.0
45	34.52360338223603	37.0	34.0	40.0	23.0	41.0
46	34.331987906819876	37.0	34.0	40.0	23.0	41.0
47	34.114951458649514	36.0	33.0	40.0	22.0	41.0
48	34.13151815181518	36.0	33.0	40.0	23.0	41.0
49	34.05304858953048	36.0	33.0	40.0	23.0	41.0
50	33.91713883067139	35.0	33.0	40.0	23.0	41.0
51	33.05542265905423	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	37.0
10	39.0
11	37.0
12	34.0
13	36.0
14	51.0
15	69.0
16	94.0
17	183.0
18	408.0
19	893.0
20	2065.0
21	4016.0
22	5946.0
23	7584.0
24	9202.0
25	12125.0
26	14322.0
27	14440.0
28	13813.0
29	14404.0
30	16557.0
31	20338.0
32	25780.0
33	34630.0
34	55318.0
35	67392.0
36	75534.0
37	109730.0
38	156602.0
39	168469.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.939148659391485	18.817060538170605	20.521548505215485	14.722242297222424
2	33.269976632699766	31.47189901471899	20.674158656741586	14.583965695839657
3	27.50102382501024	30.24258630242586	28.33911493339115	13.91727493917275
4	25.11936595119366	21.357230613572305	37.65291127652911	15.870492158704922
5	28.63566283635663	29.319457493194573	26.092361060923608	15.952518609525185
6	22.30372672303727	38.354291633542914	26.8978102189781	12.444171424441715
7	67.62581002625811	5.547445255474453	22.54920382549204	4.277540892775409
8	66.82758786827588	13.299968682999685	14.632747946327479	5.239695502396955
9	61.98658186986582	7.429837874298379	16.182578111825784	14.401002144010022
10	39.416178844161784	21.861554768615548	24.0337500903375	14.688516296885162
11	30.782443207824432	22.14160102141601	28.93654693936547	18.13940883139409
12	24.802221098022212	19.843053648430537	32.2002601720026	23.15446508154465
13	24.80944809809448	22.639541326395413	35.636216906362165	16.914793669147937
14	18.75430608754306	30.082026450820265	30.577919105779188	20.585748355857483
15	17.301197273011972	24.255859892558597	37.577027775770276	20.86591505865915
16	19.097347690973475	24.070607790706077	31.237262412372623	25.59478210594782
17	19.050974440509744	28.92076798920768	31.801570668015707	20.22668690226687
18	18.87258798872588	25.297993302979933	32.68976897689769	23.139649731396496
19	19.608898846088987	27.14557587145576	29.55445544554455	23.691069836910696
20	22.419358724193586	26.116451061164508	34.92905494929055	16.535135265351354
21	20.216448652164487	31.11187396111874	31.33109296331093	17.340584423405843
22	18.67264098672641	23.434270434342704	34.904844499048444	22.988244079882442
23	21.42913926429139	26.9245501192455	32.00561297005613	19.640697646406977
24	20.40097805400978	26.556936715569368	31.175712461757126	21.866372768663727
25	17.624364626243647	32.64496157644962	28.846329888463302	20.88434390884344
26	18.316229433162295	25.713425357134252	33.952928139529284	22.01741707017417
27	23.192647731926478	26.822408518224083	29.701043097010434	20.28390065283901
28	16.98850906988509	27.849124328491243	33.35453253354533	21.807834068078343
29	23.313820433138204	22.623641926236417	32.46308207463082	21.599455565994553
30	20.93975090939751	30.242345402423453	30.03565320035653	18.782250487822505
31	21.64859916648599	25.79063380790634	26.473344414733447	26.087422610874228
32	26.683891016838913	28.03244923032449	26.055262460552626	19.228397292283972
33	22.822384428223845	28.212401532124016	25.997446459974466	22.967767579677677
34	21.76555611765556	27.980173929801737	26.900821469008214	23.353448483534482
35	25.330153453301534	27.415865674158656	26.33265881332659	20.92132205921322
36	22.067403820674038	31.700031317000317	25.140565151405653	21.09199971092
37	23.118691431186914	28.019681530196817	28.47775288477753	20.38387415383874
38	19.129748741297487	30.492038254920384	25.214039652140396	25.164173351641733
39	22.997277829972777	30.79412685794127	26.809881718098815	19.398713593987136
40	19.665148996651492	30.833273108332733	28.110500831105007	21.39107706391077
41	18.4180096841801	32.13919202139192	26.131266411312666	23.311531883115318
42	19.94326804943268	29.235624292356242	29.658524246585245	21.162583411625835
43	22.099443520994434	28.33875358338754	26.8603502686035	22.701452627014525
44	21.650887716508876	25.594059405940595	28.73154103731541	24.023511840235116
45	20.447471754474716	25.867842258678426	27.427790224277903	26.25689576256896
46	24.923875599238755	29.06217629062176	26.026474910264746	19.98747319987473
47	19.058201440582014	28.105923731059235	32.67916937679169	20.156705451567056
48	20.176941051769408	27.67856712678567	28.9106501891065	23.233841632338418
49	20.376044903760448	25.28558695285587	32.67025607670256	21.668112066681118
50	20.476018404760186	25.530100455301003	29.798125797981257	24.195755341957554
51	19.914841849148416	24.323552793235528	27.986798679867984	27.774806677748064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	345.0
1	1021.0
2	1697.0
3	9446.5
4	17196.0
5	11460.5
6	5725.0
7	5498.5
8	5272.0
9	5211.0
10	5150.0
11	5041.0
12	4932.0
13	4839.0
14	4746.0
15	4540.0
16	4334.0
17	4230.0
18	4126.0
19	4151.0
20	4176.0
21	4826.0
22	5476.0
23	5310.5
24	5145.0
25	5762.5
26	7385.5
27	8391.0
28	9591.0
29	10791.0
30	13164.5
31	15538.0
32	16658.0
33	17778.0
34	20219.0
35	22660.0
36	23831.5
37	25003.0
38	26257.0
39	27511.0
40	31398.5
41	35286.0
42	38836.0
43	42386.0
44	47337.0
45	52288.0
46	82934.5
47	113581.0
48	95408.0
49	77235.0
50	75760.0
51	74285.0
52	64831.0
53	55377.0
54	48633.5
55	41890.0
56	37125.0
57	32360.0
58	29865.5
59	27371.0
60	25161.0
61	22951.0
62	19947.5
63	16944.0
64	14657.0
65	12370.0
66	10420.0
67	8470.0
68	7258.0
69	6046.0
70	4952.0
71	3858.0
72	3123.5
73	2389.0
74	1927.0
75	1051.5
76	638.0
77	502.5
78	367.0
79	271.0
80	175.0
81	137.5
82	100.0
83	54.0
84	8.0
85	5.0
86	2.0
87	3.5
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	830220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.54424517893208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5094364645307	27.55014413376138
2	9.831973100388792	6.989401249457686
3	3.41823935102704	3.644962127195364
4	1.7053043095278584	2.4245501793019075
5	1.0192532362167288	1.8114293463753695
6	0.7337056153819947	1.5647407369378117
7	0.5361545777661771	1.3340046836149466
8	0.4300605523533045	1.222894217170625
9	0.34385351659252983	1.0999812329462582
>10	4.16984585011641	33.67841916051502
>50	0.24682653359283163	5.468992939600219
>100	0.04434626790828569	2.9070289649481214
>500	0.006187851336039825	1.4512539622835827
>1k	0.0034376951866887913	3.0683201352953646
>5k	0.0	0.0
>10k+	0.0013750780746755165	5.783876930596396
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	13416	1.6159572161595719	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	11696	1.408783214087832	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	11599	1.3970995639709958	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10624	1.279660812796608	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	4988	0.6008046060080461	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT	3686	0.44397870443978704	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT	3033	0.36532485365324857	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT	3007	0.3621931536219315	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA	2410	0.290284502902845	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC	2202	0.26523090265230903	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC	2106	0.25366770253667703	No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	1609	0.1938040519380405	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	1048	0.12623160126231603	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1022	0.12309990123099901	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT	953	0.11478885114788852	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	913	0.10997085109970851	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	857	0.1032256510322565	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20464455204644552	0.0
2	0.0	0.0	0.0	1.1412637614126375	0.0
3	0.0	0.0	0.0	1.442027414420274	0.0
4	0.0	0.0	0.0	2.2314567223145674	0.0
5	0.0	0.0	0.0	4.615041796150418	0.0
6	0.0	0.0	0.0	5.424224904242249	0.0
7	0.0	0.0	0.0	6.4857507648575075	0.0
8	0.0	0.0	0.0	7.742646527426465	0.0
9	0.0	0.0	0.0	8.147358531473586	0.0
10	0.0	0.0	0.0	10.855556358555564	0.0
11	0.0	0.0	0.0	12.413938474139385	0.0
12	0.0	0.0	0.0	14.998675049986751	0.0
13	0.0	0.0	0.0	15.534316205343162	0.0
14	0.0	0.0	0.0	15.779070607790706	0.0
15	0.0	0.0	0.0	16.41143311411433	0.0
16	0.0	0.0	0.0	17.13148322131483	0.0
17	0.0	0.0	0.0	17.985714629857146	0.0
18	0.0	0.0	0.0	18.815976488159766	0.0
19	0.0	0.0	0.0	19.804027848040278	0.0
20	0.0	0.0	0.0	20.387126303871263	0.0
21	0.0	0.0	0.0	21.065741610657415	0.0
22	0.0	0.0	0.0	21.796511767965118	0.0
23	0.0	0.0	0.0	22.511623425116234	0.0
24	0.0	0.0	0.0	23.068102430681023	0.0
25	0.0	0.0	0.0	23.593264435932646	0.0
26	0.0	0.0	0.0	24.071089590710894	0.0
27	0.0	0.0	0.0	24.59119269591193	0.0
28	0.0	0.0	0.0	25.121413601214137	0.0
29	0.0	0.0	0.0	25.6236901062369	0.0
30	0.0	0.0	0.0	26.14692491146925	0.0
31	0.0	0.0	0.0	26.66546216665462	0.0
32	0.0	0.0	0.0	27.186649321866494	0.0
33	0.0	0.0	0.0	27.684830526848305	0.0
34	0.0	0.0	0.0	28.144226831442268	0.0
35	0.0	0.0	0.0	28.650598636505986	0.0
36	0.0	0.0	0.0	29.136373491363734	0.0
37	0.0	0.0	0.0	29.63973404639734	0.0
38	0.0	0.0	0.0	30.127074751270747	0.0
39	0.0	0.0	0.0	30.603936306039362	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	30	2.1647702E-6	45.000004	18
CGTATAT	30	2.1647702E-6	45.000004	22
CGTTCGG	20	7.032153E-4	45.000004	45
CCGTATA	30	2.1647702E-6	45.000004	21
TCCGTAT	30	2.1647702E-6	45.000004	20
CTACCCG	20	7.032153E-4	45.000004	34
CCGCGTA	20	7.032153E-4	45.000004	40
TCGACCG	20	7.032153E-4	45.000004	24
CGTCATA	30	2.1647702E-6	45.000004	38
GATTACG	20	7.032153E-4	45.000004	1
TCGAATA	20	7.032153E-4	45.000004	44
CCCTACG	25	3.889866E-5	45.0	32
GTACTAG	45	3.8380676E-10	45.0	1
CGATGCA	35	1.2113742E-7	45.0	10
GACGATA	25	3.889866E-5	45.0	22
CGATGAA	2295	0.0	42.941177	19
CGTTTTT	5540	0.0	42.847473	1
CCGATGA	2245	0.0	42.694874	18
GATGAAT	2495	0.0	42.65531	20
CGTAAGC	80	0.0	42.187504	43
>>END_MODULE