FastQCFastQC Report
Sat 18 Jun 2016
SRR3551320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938091
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.6731756300827958No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG40990.43695121262222963No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC40170.4282100563804577No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC36080.38461087463796156No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC29840.318092807627405TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC25600.2728946338894628No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC19080.20339178182074022TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT17930.191132843188987TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC11270.12013759859118146TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT9740.10382788023763152No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT9720.10361468130490538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG302.165074E-645.0000041
TTCGGTA253.8902544E-545.00000425
CCAACGG253.8902544E-545.0000042
TATCCGA253.8902544E-545.0000042
GTACCCG207.0326234E-445.022
TTTCGGC207.0326234E-445.024
GTTCGAC207.0326234E-445.012
CAATGCG351.2116107E-745.01
CGACTAA207.0326234E-445.029
CGTTATT2050.042.804881
TAGTGCG650.041.5384641
CGTTTTT34100.041.3709681
CCCCGAT501.0804797E-940.50000440
CGTAAGG1300.039.8076932
CGTTGAT403.4573168E-739.37525
TGCGTAG800.039.3751
CGGGATC2250.039.0000046
CGGTCTA356.247061E-638.57142631
CGCCACG356.247061E-638.57142616
ATCGAAT659.094947E-1238.07692743
Produced by FastQC (version 0.11.5)