##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	938091
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623799823258086	31.0	31.0	34.0	30.0	34.0
2	31.757577889565084	33.0	31.0	34.0	30.0	34.0
3	31.694158669041702	33.0	31.0	34.0	30.0	34.0
4	35.38411518711937	37.0	35.0	37.0	33.0	37.0
5	33.41684868525548	37.0	35.0	37.0	30.0	37.0
6	34.33617527510657	37.0	35.0	37.0	30.0	37.0
7	35.43103174425509	37.0	35.0	37.0	32.0	37.0
8	35.3743453460272	37.0	35.0	37.0	33.0	37.0
9	37.26616714156729	39.0	37.0	39.0	34.0	39.0
10	36.620591179320556	38.0	35.0	39.0	32.0	39.0
11	36.770595816397346	39.0	37.0	39.0	33.0	39.0
12	36.85223181972751	39.0	37.0	39.0	33.0	39.0
13	36.99708130661098	39.0	37.0	39.0	33.0	39.0
14	38.043701517230204	40.0	37.0	41.0	33.0	41.0
15	38.110769637487195	40.0	37.0	41.0	33.0	41.0
16	38.16829177553137	40.0	37.0	41.0	34.0	41.0
17	38.05254927293834	40.0	37.0	41.0	33.0	41.0
18	37.999915786421575	39.0	37.0	41.0	33.0	41.0
19	37.88409013624478	39.0	37.0	41.0	33.0	41.0
20	38.02351157830104	40.0	37.0	41.0	34.0	41.0
21	37.907339479858564	40.0	36.0	41.0	33.0	41.0
22	38.067456142314555	40.0	37.0	41.0	34.0	41.0
23	38.13659016022966	40.0	37.0	41.0	34.0	41.0
24	38.007461962645415	40.0	37.0	41.0	34.0	41.0
25	37.722955448885024	39.0	36.0	41.0	33.0	41.0
26	37.831172029152825	40.0	36.0	41.0	33.0	41.0
27	37.483974369224306	40.0	36.0	41.0	33.0	41.0
28	37.52105605959336	40.0	36.0	41.0	33.0	41.0
29	37.737048964332885	40.0	36.0	41.0	33.0	41.0
30	37.44386312202121	40.0	36.0	41.0	33.0	41.0
31	37.361761279023035	39.0	36.0	41.0	33.0	41.0
32	37.43405064114249	40.0	36.0	41.0	33.0	41.0
33	37.412549528777056	40.0	36.0	41.0	33.0	41.0
34	37.270047362142904	40.0	36.0	41.0	32.0	41.0
35	37.26678008849888	40.0	36.0	41.0	32.0	41.0
36	37.02136359905382	40.0	36.0	41.0	31.0	41.0
37	36.97974183741236	40.0	36.0	41.0	31.0	41.0
38	36.796805427192034	40.0	35.0	41.0	31.0	41.0
39	36.80191154163082	39.0	35.0	41.0	31.0	41.0
40	36.76586173409616	39.0	35.0	41.0	31.0	41.0
41	36.633089966751626	39.0	35.0	41.0	30.0	41.0
42	36.79097443638197	39.0	35.0	41.0	31.0	41.0
43	36.69480892578652	39.0	35.0	41.0	31.0	41.0
44	36.74830480198616	39.0	35.0	41.0	31.0	41.0
45	36.72948146821577	39.0	35.0	41.0	31.0	41.0
46	36.6114097672827	39.0	35.0	41.0	31.0	41.0
47	36.532930174151545	39.0	35.0	41.0	31.0	41.0
48	36.50962433282059	39.0	35.0	41.0	31.0	41.0
49	36.46733419252503	39.0	35.0	41.0	31.0	41.0
50	36.39543605044713	39.0	35.0	41.0	31.0	41.0
51	35.401129527945585	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	17.0
9	48.0
10	47.0
11	42.0
12	31.0
13	32.0
14	55.0
15	66.0
16	98.0
17	141.0
18	281.0
19	465.0
20	923.0
21	1508.0
22	2480.0
23	3348.0
24	5174.0
25	7179.0
26	9193.0
27	10268.0
28	10423.0
29	11613.0
30	14015.0
31	17440.0
32	23001.0
33	32564.0
34	53945.0
35	66821.0
36	76899.0
37	115133.0
38	201899.0
39	272855.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.970012504117406	20.73551499801192	25.339972348098428	12.95450014977225
2	33.64129919165625	26.024554121082073	24.003748037237326	16.330398650024357
3	30.73294595087257	24.659974352168394	28.48806778873265	16.119011908226387
4	28.201528423148716	24.611684793905923	29.44938177639483	17.73740500655054
5	24.618080761887708	32.74959465552915	25.167387812056614	17.464936770526528
6	25.274307076818776	34.49249593056537	26.488155200295065	13.745041792320785
7	76.7957479604857	6.026387631903515	12.900347620859808	4.277516786750966
8	77.52137052801913	6.8811021532026215	11.05532405704777	4.542203261730472
9	71.38113466603986	7.894543279916341	13.100861217088747	7.62346083695505
10	36.701130274141846	28.1614470237962	21.666341538294258	13.471081163767696
11	26.65711535448054	25.189667100526496	28.77940413030292	19.373813414690048
12	24.369810604728112	22.226628333498564	32.9448848779063	20.458676183867023
13	23.517867669554445	23.769762208570384	34.85695950606071	17.855410615814456
14	20.203690260326557	27.469403288167136	31.876012028683785	20.45089442282252
15	18.679424490801	27.45757074740084	35.09989968990215	18.76310507189601
16	20.88656644184839	26.61980554125346	31.923128992816263	20.570499024081883
17	21.40250785904566	27.02008653744679	30.267959078596853	21.309446524910697
18	20.98538414716696	26.364713018246633	32.579568506680054	20.070334327906355
19	21.07226271225286	28.39543285246314	30.377969727883542	20.154334707400455
20	22.432685101978382	27.354702262360476	32.11916541145795	18.093447224203196
21	21.90277915468755	28.349808280859744	31.936560525578013	17.810852038874696
22	20.881023269597513	24.898117560023493	31.543954690962817	22.676904479416176
23	19.439585285436063	27.44925598902452	31.49214735031036	21.619011375229054
24	20.488310835515957	26.297875152836987	31.515172835044787	21.69864117660227
25	19.55268731924728	29.420599920476796	29.46046812089659	21.566244639379335
26	19.685083856470214	28.41941773239483	30.980043513902167	20.915454897232785
27	21.099871974040898	28.980450723863676	30.103156303599544	19.816520998495882
28	18.758734493775126	27.647530996459828	32.470304053657905	21.123430456107137
29	21.750555116721085	25.872010284716513	30.941454507078735	21.43598009148366
30	22.833712294436253	26.9022941271156	30.069790670627906	20.194202907820245
31	21.83338290208519	27.358539843149543	29.10240051338303	21.70567674138223
32	22.12312025166002	29.31815783330189	28.48113882341905	20.077583091619044
33	22.899697364114996	27.329651387765153	28.244488008093033	21.526163240026822
34	21.01597819401316	26.466622108089727	29.4768844387165	23.040515259180612
35	22.808128422509117	26.36780440277116	29.031511868251584	21.792555306468138
36	23.595578680533123	27.970847177939028	28.51898163397794	19.91459250754991
37	21.026211742784014	28.241503223034865	30.666321284395647	20.065963749785467
38	20.487244840852327	28.84208461652441	29.178832330765353	21.49183821185791
39	21.767184633473725	27.83280086899885	28.801257020907357	21.598757476620072
40	22.30369974767906	25.396789863669945	29.969267373847526	22.330243014803468
41	20.342376166064913	26.829593291055986	29.451940163587544	23.37609037929156
42	20.947114938742615	26.87308587333212	31.12821677214684	21.051582415778427
43	22.149876717717152	26.790471286900736	29.832926656369157	21.22672533901295
44	21.143151357384305	26.914553065747356	29.035136250107936	22.90715932676041
45	22.054896593187653	25.835233468821254	28.209843181525034	23.90002675646606
46	23.091363204635798	26.918177447603696	28.413661361211222	21.57679798654928
47	20.42552374982811	26.244895218054538	31.615696131825167	21.71388490029219
48	20.744256154253694	25.943431927179773	30.61771192773409	22.694599990832444
49	21.591508712907384	24.843325434312877	31.802458396893265	21.762707455886478
50	20.81184021592788	24.585461325180606	30.499493119537448	24.103205339354073
51	20.47893008247601	24.2812264481804	28.93439975439483	26.305443714948762
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	473.0
1	1032.0
2	1591.0
3	6075.0
4	10559.0
5	7180.0
6	3801.0
7	3754.5
8	3708.0
9	3677.5
10	3647.0
11	3666.5
12	3686.0
13	3686.5
14	3687.0
15	3597.5
16	3508.0
17	3524.0
18	3540.0
19	3751.5
20	3963.0
21	4373.5
22	4784.0
23	5286.5
24	5789.0
25	7174.0
26	9749.5
27	10940.0
28	12265.0
29	13590.0
30	16278.0
31	18966.0
32	20449.5
33	21933.0
34	26128.0
35	30323.0
36	32815.5
37	35308.0
38	36418.5
39	37529.0
40	42071.5
41	46614.0
42	51830.5
43	57047.0
44	61965.0
45	66883.0
46	81692.5
47	96502.0
48	96538.0
49	96574.0
50	94493.5
51	92413.0
52	80142.0
53	67871.0
54	57984.5
55	48098.0
56	42238.5
57	36379.0
58	32592.0
59	28805.0
60	26605.5
61	24406.0
62	21295.5
63	18185.0
64	14783.0
65	11381.0
66	9152.5
67	6924.0
68	5650.0
69	4376.0
70	3512.0
71	2648.0
72	2083.5
73	1519.0
74	1116.0
75	620.5
76	528.0
77	380.0
78	232.0
79	154.5
80	77.0
81	45.5
82	14.0
83	10.5
84	7.0
85	5.0
86	3.0
87	5.0
88	7.0
89	4.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	938091.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.64834459464869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03467909562367	24.911443941637792
2	10.995554409745923	7.399644075766795
3	4.000656684754926	4.038464242005557
4	2.004336236596823	2.697703854902048
5	1.205454110714247	2.0280767655174388
6	0.7819805989308747	1.5787411595493506
7	0.6022431454962016	1.4185139422594937
8	0.4574582235730916	1.2314169555554584
9	0.4186284451493629	1.2677538761556977
>10	5.081295733860784	39.11084173078116
>50	0.36745972564074747	7.606481593672076
>100	0.0438518587142051	2.735569545041596
>500	0.0035209521595314765	0.9153748400841176
>1k	0.0025606924796592557	2.379823798858532
>5k	3.2008655995740697E-4	0.6801496782128724
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6315	0.6731756300827958	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	4099	0.43695121262222963	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	4017	0.4282100563804577	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	3608	0.38461087463796156	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	2984	0.318092807627405	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC	2560	0.2728946338894628	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	1908	0.20339178182074022	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	1793	0.191132843188987	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	1127	0.12013759859118146	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	974	0.10382788023763152	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	972	0.10361468130490538	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0659946636307138E-4	0.0	0.0	0.19838160690167586	0.0
2	1.0659946636307138E-4	0.0	0.0	0.9468164602368001	0.0
3	1.0659946636307138E-4	0.0	0.0	1.2997672933649294	0.0
4	1.0659946636307138E-4	0.0	0.0	1.8266884555975913	0.0
5	1.0659946636307138E-4	0.0	0.0	3.4069189449637616	0.0
6	1.0659946636307138E-4	0.0	0.0	4.218780480784913	0.0
7	1.0659946636307138E-4	0.0	0.0	5.073068604218567	0.0
8	1.0659946636307138E-4	0.0	0.0	6.239799763562384	0.0
9	1.0659946636307138E-4	0.0	0.0	6.688903315350004	0.0
10	1.0659946636307138E-4	0.0	0.0	8.237900161071794	0.0
11	1.0659946636307138E-4	0.0	0.0	10.065228213467563	0.0
12	1.0659946636307138E-4	0.0	0.0	11.751311972932264	0.0
13	1.0659946636307138E-4	0.0	0.0	12.32140591904197	0.0
14	1.0659946636307138E-4	0.0	0.0	12.56157451675797	0.0
15	1.0659946636307138E-4	0.0	0.0	13.014728848267385	0.0
16	1.0659946636307138E-4	0.0	0.0	13.801326310560489	0.0
17	1.0659946636307138E-4	0.0	0.0	14.787691172817988	0.0
18	1.0659946636307138E-4	0.0	0.0	15.823837985867042	0.0
19	1.0659946636307138E-4	0.0	0.0	16.59146074314752	0.0
20	1.0659946636307138E-4	0.0	0.0	17.23734690984137	0.0
21	2.1319893272614277E-4	0.0	0.0	18.02138598494176	0.0
22	2.1319893272614277E-4	0.0	0.0	18.86277557294548	0.0
23	2.1319893272614277E-4	0.0	0.0	19.661098976538522	0.0
24	2.1319893272614277E-4	0.0	0.0	20.28992922861428	0.0
25	2.1319893272614277E-4	0.0	0.0	20.84691144036133	0.0
26	2.1319893272614277E-4	0.0	0.0	21.371167615934915	0.0
27	2.1319893272614277E-4	0.0	0.0	21.88636283686764	0.0
28	2.1319893272614277E-4	0.0	0.0	22.429380518521125	0.0
29	2.1319893272614277E-4	0.0	0.0	22.98273834841183	0.0
30	2.1319893272614277E-4	0.0	0.0	23.568822214475993	0.0
31	2.1319893272614277E-4	0.0	0.0	24.14659132216384	0.0
32	2.1319893272614277E-4	0.0	0.0	24.688116611288244	0.0
33	2.1319893272614277E-4	0.0	0.0	25.22910890308083	0.0
34	2.1319893272614277E-4	0.0	0.0	25.75762905730894	0.0
35	2.1319893272614277E-4	0.0	0.0	26.30437772028513	0.0
36	2.1319893272614277E-4	0.0	0.0	26.841212632889558	0.0
37	2.1319893272614277E-4	0.0	0.0	27.36941298871858	0.0
38	3.1979839908921414E-4	0.0	0.0	27.897293546148507	0.0
39	3.1979839908921414E-4	0.0	0.0	28.43679344541201	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	30	2.165074E-6	45.000004	1
TTCGGTA	25	3.8902544E-5	45.000004	25
CCAACGG	25	3.8902544E-5	45.000004	2
TATCCGA	25	3.8902544E-5	45.000004	2
GTACCCG	20	7.0326234E-4	45.0	22
TTTCGGC	20	7.0326234E-4	45.0	24
GTTCGAC	20	7.0326234E-4	45.0	12
CAATGCG	35	1.2116107E-7	45.0	1
CGACTAA	20	7.0326234E-4	45.0	29
CGTTATT	205	0.0	42.80488	1
TAGTGCG	65	0.0	41.538464	1
CGTTTTT	3410	0.0	41.370968	1
CCCCGAT	50	1.0804797E-9	40.500004	40
CGTAAGG	130	0.0	39.807693	2
CGTTGAT	40	3.4573168E-7	39.375	25
TGCGTAG	80	0.0	39.375	1
CGGGATC	225	0.0	39.000004	6
CGGTCTA	35	6.247061E-6	38.571426	31
CGCCACG	35	6.247061E-6	38.571426	16
ATCGAAT	65	9.094947E-12	38.076927	43
>>END_MODULE