FastQCFastQC Report
Sat 18 Jun 2016
SRR3551319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888647
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.5887602163738808No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC47210.5312570683297192TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG42280.4757794714886789No Hit
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC39800.4478718771345653No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC37290.4196266909132647No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT26770.30124447615307315TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC22880.25747006404117717TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC22510.2533064310125393No Hit
TCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC17350.19524062985639967TruSeq Adapter, Index 22 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13670.15382936081481174No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC12970.14595221724711838No Hit
ACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC11440.12873503202058859TruSeq Adapter, Index 23 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT10790.12142054156487334No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT9470.10656649940865158No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCG302.164943E-645.00000417
CGACGTT351.2115197E-745.00000427
CGATTGC351.2115197E-745.00000410
CCTGTCG351.2115197E-745.00000440
TATAGCG302.164943E-645.0000041
TCGACGT351.2115197E-745.00000426
CGAGTAT207.032422E-445.032
ATTACGT253.8900893E-545.013
TAGCCGT253.8900893E-545.044
CGTACTC207.032422E-445.022
ACGTCCA253.8900893E-545.016
ACGTACG207.032422E-445.01
CCACGCG207.032422E-445.01
TAATGCG207.032422E-445.01
ATACCGA253.8900893E-545.025
TACGTCC253.8900893E-545.015
AATCGCT207.032422E-445.032
GTACGAG406.8121153E-945.01
CGTTAGG1000.042.752
CGCACGG900.042.52