FastQCFastQC Report
Sat 18 Jun 2016
SRR3551317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986712
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107961.0941389179416081No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC27400.277689943975547No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC25730.2607650459303221Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG23620.23938089331030737No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC20960.21242267247180538Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT20700.2097876584048841No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC18640.18891023925927727No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT18290.18536310493842176TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC17760.17999173010969766No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT13220.13398033063345738No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCC11770.11928505987562733No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG11560.11715677928311402TruSeq Adapter, Index 27 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11160.11310291148785055No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT10350.10489382920244204TruSeq Adapter, Index 27 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10140.10276554860992873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCG302.1651886E-645.00000410
CGCGTAA253.890402E-545.00000431
CGATACG302.1651886E-645.00000410
AAATGCG253.890402E-545.0000041
GCTATCG207.032802E-445.01
CGGATCG207.032802E-445.02
CGTACGA207.032802E-445.013
TTACGAT207.032802E-445.013
CGACTTA207.032802E-445.024
CGTTTTT57600.043.242191
CGAATAT1150.041.08695614
TAGACGG950.040.2631572
ACTACGG750.039.02
CGATGAA4500.039.019
CGTTCAC356.247392E-638.57142620
ACGTGCG356.247392E-638.5714261
TGTCGCG356.247392E-638.5714261
TACGGCT2750.038.4545447
ACGGGAT3900.038.0769235
ACGGGTA950.037.8947375
Produced by FastQC (version 0.11.5)