##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551317_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	986712
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.556807862881975	31.0	31.0	34.0	30.0	34.0
2	31.734472672877192	33.0	31.0	34.0	30.0	34.0
3	31.65842211303805	33.0	31.0	34.0	30.0	34.0
4	35.371027209560644	37.0	35.0	37.0	33.0	37.0
5	33.35683157800858	37.0	35.0	37.0	30.0	37.0
6	34.275031620168804	37.0	35.0	37.0	30.0	37.0
7	35.35527894664299	37.0	35.0	37.0	32.0	37.0
8	35.31297886313332	37.0	35.0	37.0	33.0	37.0
9	37.23286430082942	39.0	37.0	39.0	34.0	39.0
10	36.619304315747655	39.0	35.0	39.0	32.0	39.0
11	36.73962817924582	39.0	37.0	39.0	32.0	39.0
12	36.77203378493421	39.0	35.0	39.0	33.0	39.0
13	36.89843338279052	39.0	37.0	39.0	33.0	39.0
14	37.94297626865793	40.0	37.0	41.0	33.0	41.0
15	38.05192599258953	40.0	37.0	41.0	33.0	41.0
16	38.08035171356992	40.0	37.0	41.0	33.0	41.0
17	37.96525125872595	40.0	37.0	41.0	33.0	41.0
18	37.885344457146566	39.0	37.0	41.0	33.0	41.0
19	37.706073302037474	39.0	37.0	41.0	33.0	41.0
20	37.810522219249386	39.0	36.0	41.0	34.0	41.0
21	37.693514419607745	39.0	36.0	41.0	33.0	41.0
22	37.823736814795	39.0	36.0	41.0	33.0	41.0
23	37.90087685160412	40.0	36.0	41.0	34.0	41.0
24	37.73875355726899	40.0	36.0	41.0	33.0	41.0
25	37.42578685573906	39.0	35.0	41.0	33.0	41.0
26	37.535001094544306	39.0	35.0	41.0	33.0	41.0
27	37.188311280292524	39.0	35.0	41.0	33.0	41.0
28	37.23832688768354	39.0	35.0	41.0	33.0	41.0
29	37.4560945848434	40.0	35.0	41.0	33.0	41.0
30	37.17210695724791	39.0	35.0	41.0	32.0	41.0
31	37.10988414045841	39.0	35.0	41.0	32.0	41.0
32	37.1003524838048	39.0	35.0	41.0	32.0	41.0
33	37.003384979609045	40.0	35.0	41.0	32.0	41.0
34	36.83081790836637	40.0	35.0	41.0	31.0	41.0
35	36.74233008213136	40.0	35.0	41.0	31.0	41.0
36	36.496855212057824	40.0	35.0	41.0	30.0	41.0
37	36.50121311993773	40.0	35.0	41.0	30.0	41.0
38	36.33261883913442	39.0	35.0	41.0	30.0	41.0
39	36.26538442828303	39.0	35.0	41.0	30.0	41.0
40	36.1827240370037	39.0	35.0	41.0	30.0	41.0
41	36.02786223335685	39.0	35.0	41.0	29.0	41.0
42	36.17351060897202	39.0	35.0	41.0	30.0	41.0
43	36.107949432053125	39.0	35.0	41.0	30.0	41.0
44	36.16229051638168	39.0	35.0	41.0	30.0	41.0
45	36.15611140839475	39.0	35.0	41.0	30.0	41.0
46	36.031368828999746	39.0	35.0	41.0	29.0	41.0
47	35.965257339527646	39.0	35.0	41.0	29.0	41.0
48	35.92634730296176	39.0	35.0	41.0	29.0	41.0
49	35.89798340346525	39.0	35.0	41.0	29.0	41.0
50	35.830489545074954	39.0	35.0	41.0	29.0	41.0
51	34.95978157760319	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	6.0
8	24.0
9	55.0
10	64.0
11	71.0
12	61.0
13	56.0
14	74.0
15	106.0
16	147.0
17	256.0
18	423.0
19	703.0
20	1215.0
21	1894.0
22	2898.0
23	4340.0
24	6411.0
25	9697.0
26	12971.0
27	14144.0
28	13635.0
29	13972.0
30	15560.0
31	19615.0
32	25690.0
33	35944.0
34	61690.0
35	75754.0
36	82845.0
37	118996.0
38	198461.0
39	268864.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.33670615133899	21.517626217173806	23.480002270165965	15.665665361321238
2	35.7558233810879	24.164193807311555	23.465002959323492	16.614979852277056
3	29.803630643997437	23.104107378850163	30.8655413129667	16.226720664185702
4	27.777406173229878	23.862484696649073	30.35688225135602	18.00322687876503
5	23.727896285846324	31.8468813595051	27.09706581048979	17.328156544158784
6	25.15202004232238	32.923385952537316	27.31749487185724	14.607099133283066
7	75.11553523216502	5.899897842531559	14.135026228524636	4.849540696778797
8	76.19801928019524	6.324743187475169	12.692964107054541	4.784273425275055
9	70.30764802698253	7.680964658380561	15.081300318634009	6.930086996002887
10	38.89301032114741	24.59572803411735	22.374917909177146	14.136343735558096
11	28.244918476718638	24.597754968014986	27.911386503863334	19.245940051403043
12	25.68409019045071	21.03308766894494	32.47695376158393	20.805868379020424
13	23.127113078588284	23.49226522024664	33.813108586902764	19.567513114262315
14	19.738687681917316	26.728467881205457	32.36385085009608	21.16899358678115
15	19.402115308215567	26.005156519835577	34.773267174210915	19.81946099773794
16	21.83149693122208	25.162965485369593	32.357567355013416	20.647970228394914
17	21.790046133015508	26.48462773332036	29.63032779574992	22.094998337914205
18	21.905784058570283	25.724122134928933	31.771175378428556	20.598918428072224
19	22.712301056437948	26.97960499112203	29.338956047965365	20.96913790447466
20	23.47007029406757	26.14562303894146	31.12823194609977	19.256074720891203
21	23.248019685582015	26.812078904482767	30.944997121753865	18.994904288181356
22	21.466648829648367	24.446748392641418	31.074315504422767	23.012287273287445
23	21.64055975806517	25.55639335489991	31.444636327520087	21.358410559514834
24	21.770080834123835	24.702648797217424	32.07602623663237	21.451244132026368
25	20.766444514711484	27.727239559263495	29.425404778699356	22.08091114732566
26	19.896788525932593	27.091086355491772	30.410190612863737	22.6019345057119
27	21.192404673298796	27.617582435401616	30.492788169192224	20.697224722107364
28	19.462416591670113	26.123934846236796	32.61458257323312	21.799065988859972
29	19.999655421237403	24.277094025409642	31.31896642586692	24.404284127486033
30	19.711324074299288	26.27848855593121	31.792863571133218	22.21732379863628
31	20.694184321260913	25.419879356894416	31.164514062867383	22.721422258977288
32	20.927079026098802	25.787869206009457	30.20526759581317	23.07978417207858
33	20.35913214798239	25.17056648748571	30.579338246621102	23.8909631179108
34	19.198408451503578	24.779165551853023	31.895021039573855	24.12740495706954
35	21.357903826040424	24.455970941875645	30.093482191358778	24.092643040725157
36	20.956570914309342	26.207545869514103	30.126217173805525	22.709666042371026
37	20.544089866141285	25.850400116751395	31.220964171916428	22.3845458451909
38	20.123095695603173	25.927220911471633	29.495942078336938	24.45374131458825
39	20.62222816789499	24.75403157152239	30.829765929673503	23.79397433090912
40	22.28502339081718	24.31580846285441	31.24964528656791	22.1495228597605
41	20.037052351648708	24.443707991794973	30.139392244140133	25.379847412416183
42	20.31190458816757	24.964731350181207	30.08213136153204	24.641232700119183
43	21.106158635954564	24.21942775602202	29.838696600426466	24.835717007596948
44	20.052963782745117	24.934428688411614	30.048180218746705	24.964427310096564
45	20.89525616390598	24.42384403959818	28.888774029301356	25.79212576719448
46	21.18074980338741	25.55933240905148	29.42753305929187	23.832384728269243
47	19.884728269241684	24.172200196207204	32.373580132804705	23.569491401746408
48	19.822400051889506	23.83299280843853	31.83846958382993	24.506137555842027
49	20.271264563520056	23.31683409140661	32.36040506247011	24.05149628260323
50	19.189996675828407	23.28490988251891	32.44786726015291	25.077226181499768
51	19.787840828934886	22.831484769618694	30.156013102100715	27.224661299345705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	344.0
1	858.0
2	1372.0
3	9321.0
4	17270.0
5	11489.5
6	5709.0
7	5505.0
8	5301.0
9	5324.5
10	5348.0
11	5310.5
12	5273.0
13	5181.5
14	5090.0
15	5036.5
16	4983.0
17	4878.0
18	4773.0
19	4632.5
20	4492.0
21	5015.0
22	5538.0
23	5149.0
24	4760.0
25	5370.0
26	7622.5
27	9265.0
28	11160.5
29	13056.0
30	14271.5
31	15487.0
32	16991.5
33	18496.0
34	22007.5
35	25519.0
36	26837.0
37	28155.0
38	30786.5
39	33418.0
40	38256.0
41	43094.0
42	48961.5
43	54829.0
44	60713.0
45	66597.0
46	79096.5
47	91596.0
48	94362.0
49	97128.0
50	94232.0
51	91336.0
52	79818.0
53	68300.0
54	60490.0
55	52680.0
56	48163.0
57	43646.0
58	41134.0
59	38622.0
60	36355.0
61	34088.0
62	30311.0
63	26534.0
64	23492.0
65	20450.0
66	17319.0
67	14188.0
68	11727.0
69	9266.0
70	7698.5
71	6131.0
72	5185.5
73	4240.0
74	3112.0
75	1576.5
76	1169.0
77	963.0
78	757.0
79	492.5
80	228.0
81	178.0
82	128.0
83	96.0
84	64.0
85	38.0
86	12.0
87	7.5
88	3.0
89	5.0
90	7.0
91	4.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	986712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.198554726584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10563584465618	25.265971165902908
2	9.867039880340572	6.551429269137467
3	3.539724958538093	3.5254125825924647
4	1.823850138020731	2.4219675448067606
5	1.1640078110135164	1.9321688508051735
6	0.8169193501499642	1.6272325051895415
7	0.6136442877207261	1.426047242798653
8	0.49975382796458745	1.3272883846001762
9	0.40736592751739925	1.2171564034588827
>10	4.473189327929024	34.44571573765126
>50	0.6363304889386938	13.29146511849041
>100	0.04450290963714926	2.6057185862908003
>500	0.003399527819497889	0.7790377298768949
>1k	0.00432667177027004	2.475725065445736
>5k	0.0	0.0
>10k+	3.090479835907172E-4	1.1076638129528458
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10796	1.0941389179416081	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	2740	0.277689943975547	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2573	0.2607650459303221	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	2362	0.23938089331030737	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	2096	0.21242267247180538	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTT	2070	0.2097876584048841	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	1864	0.18891023925927727	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	1829	0.18536310493842176	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	1776	0.17999173010969766	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	1322	0.13398033063345738	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCC	1177	0.11928505987562733	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTG	1156	0.11715677928311402	TruSeq Adapter, Index 27 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1116	0.11310291148785055	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	1035	0.10489382920244204	TruSeq Adapter, Index 27 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1014	0.10276554860992873	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0134669488158652E-4	0.0	0.0	0.19205198680060645	0.0
2	1.0134669488158652E-4	0.0	0.0	0.8551634114108271	0.0
3	1.0134669488158652E-4	0.0	0.0	1.1752162738468772	0.0
4	1.0134669488158652E-4	0.0	0.0	1.6409043368277674	0.0
5	1.0134669488158652E-4	0.0	0.0	3.072426402030177	0.0
6	1.0134669488158652E-4	0.0	0.0	4.130384549899059	0.0
7	1.0134669488158652E-4	0.0	0.0	5.021221997908204	0.0
8	1.0134669488158652E-4	0.0	0.0	6.091240402467995	0.0
9	1.0134669488158652E-4	0.0	0.0	6.5106130258879995	0.0
10	1.0134669488158652E-4	0.0	0.0	7.627149563398438	0.0
11	1.0134669488158652E-4	0.0	0.0	8.914962015258759	0.0
12	1.0134669488158652E-4	0.0	0.0	9.994304315747655	0.0
13	1.0134669488158652E-4	0.0	0.0	10.405670550272015	0.0
14	1.0134669488158652E-4	0.0	0.0	10.57978417207858	0.0
15	1.0134669488158652E-4	0.0	0.0	10.884229643502866	0.0
16	1.0134669488158652E-4	0.0	0.0	11.468087952715686	0.0
17	1.0134669488158652E-4	0.0	0.0	12.185217165697793	0.0
18	1.0134669488158652E-4	0.0	0.0	12.963559782388376	0.0
19	1.0134669488158652E-4	0.0	0.0	13.50292689254818	0.0
20	1.0134669488158652E-4	0.0	0.0	13.985742546964058	0.0
21	1.0134669488158652E-4	0.0	0.0	14.586829794306748	0.0
22	1.0134669488158652E-4	0.0	0.0	15.21801701002927	0.0
23	1.0134669488158652E-4	0.0	0.0	15.883560755316648	0.0
24	1.0134669488158652E-4	0.0	0.0	16.392523857011977	0.0
25	1.0134669488158652E-4	0.0	0.0	16.867130429142446	0.0
26	1.0134669488158652E-4	0.0	0.0	17.29288789433999	0.0
27	1.0134669488158652E-4	0.0	0.0	17.71894939962218	0.0
28	1.0134669488158652E-4	0.0	0.0	18.157577895069686	0.0
29	1.0134669488158652E-4	0.0	0.0	18.604212779412837	0.0
30	1.0134669488158652E-4	0.0	0.0	19.129796739068745	0.0
31	1.0134669488158652E-4	0.0	0.0	19.59771442933703	0.0
32	1.0134669488158652E-4	0.0	0.0	20.059044584438013	0.0
33	1.0134669488158652E-4	0.0	0.0	20.496254226157177	0.0
34	1.0134669488158652E-4	0.0	0.0	20.93143693397871	0.0
35	1.0134669488158652E-4	0.0	0.0	21.396111530010785	0.0
36	1.0134669488158652E-4	0.0	0.0	21.831294237832317	0.0
37	2.0269338976317304E-4	0.0	0.0	22.290597459035666	0.0
38	2.0269338976317304E-4	0.0	0.0	22.732469048719384	0.0
39	2.0269338976317304E-4	0.0	0.0	23.18579281492472	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACTCG	30	2.1651886E-6	45.000004	10
CGCGTAA	25	3.890402E-5	45.000004	31
CGATACG	30	2.1651886E-6	45.000004	10
AAATGCG	25	3.890402E-5	45.000004	1
GCTATCG	20	7.032802E-4	45.0	1
CGGATCG	20	7.032802E-4	45.0	2
CGTACGA	20	7.032802E-4	45.0	13
TTACGAT	20	7.032802E-4	45.0	13
CGACTTA	20	7.032802E-4	45.0	24
CGTTTTT	5760	0.0	43.24219	1
CGAATAT	115	0.0	41.086956	14
TAGACGG	95	0.0	40.263157	2
ACTACGG	75	0.0	39.0	2
CGATGAA	450	0.0	39.0	19
CGTTCAC	35	6.247392E-6	38.571426	20
ACGTGCG	35	6.247392E-6	38.571426	1
TGTCGCG	35	6.247392E-6	38.571426	1
TACGGCT	275	0.0	38.454544	7
ACGGGAT	390	0.0	38.076923	5
ACGGGTA	95	0.0	37.894737	5
>>END_MODULE