FastQCFastQC Report
Sat 18 Jun 2016
SRR3551315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811768
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG76650.9442352987553094No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC60070.7399897507662287No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC52690.6490770762089662No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC34070.4197011954154389TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.4078751564486405No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC20100.2476076908673414TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT18430.22703531058134835TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT18030.22210779434518238No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT14810.18244128864404607No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC14540.17911521518463402No Hit
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT12560.15472400981561235No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA12450.15336894285066668No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC12420.15299937913295425No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT10480.12910092538754914No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC8380.10323146514767767TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG302.1647102E-645.0000041
GTACGAG302.1647102E-645.0000041
CTAGACG253.8897902E-545.01
CGAAACG207.0320605E-445.012
TAGACGG750.045.02
ACCGCAT207.0320605E-445.014
ACGGGTC453.8380676E-1045.05
AGTATCG207.0320605E-445.01
TTGCGAC253.8897902E-545.029
CGTGTTA207.0320605E-445.031
CGTAAGC502.1827873E-1145.043
CCGGTTG207.0320605E-445.019
CGATCGA453.8380676E-1045.041
CGATACC207.0320605E-445.044
AGTACGG551.8189894E-1245.02
CGATGAA11300.041.61504719
CCGATGA11150.041.36771418
ACACGAC603.6379788E-1241.25000426
ACTACGG556.002665E-1140.9090922
GATGAAT12550.040.5179320